JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Fan Hou,Peipei Teng,Fengmao Liu,Wenzhuo Wang
Residue behaviors of tebuconazole and azoxystrobin in field condition and the variation of their residue levels during the boiling process were evaluated. The terminal residues of peanut kernels were determined by using a modified QuEChERS method (quick, easy, cheap, effective, rugged, and safe) by means of the optimization of the novel purification procedure with multiwalled carbon nanotubes (MWCNTs) and Fe3O4-magnetic nanoparticle (Fe3O4-MNP) in the presence of an external magnetic field, and the terminal residues were all at trace level at harvest time. The residues in shells were detected as well to investigate the distribution in peanuts. Tebuconazole and azoxystrobin residue levels varied before/after boiling in kernels and shells to different degrees due to various factors, such as the modes of action and physicochemical properties of pesticides. The residues have been transferred from peanut into the infusion during boiling with the higher percentage of azoxystrobin as its lower logKow. The processing factors (PFs) for tebuconazole and azoxystrobin after processing were <1, indicating that home cooking in this study could reduce the residue levels in peanut. Risk assessment showed there was no health risk for consumers.
研究领域:
食品科学
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农业化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Lei Liu,Huan Wang,Meng Xu,Tian-Xiu Qiu,Jiong Chen
Azoxystrobin (AZ) has entered aquatic ecosystems and produced serious damages to fish associated with potentially increasing the susceptibility to pathogens . This study characterized the defense abilities of fish by exposed to AZ on challenging with the infection of spring viraemia of carp virus (SVCV). The results showed that SVCV replication increased significantly in EPC cells and zebrafish that were exposed to up to 50 μg/L of AZ at 3, 5, 7, and 14 d. Intracellular biochemical assays indicated that AZ at 5 and 50 μg/L inhibited the activation of Nrf2-ARE pathway including a decrease in Nrf2 expression, Nrf2 phosphorylation, HO-1 content, and three antioxidant activities. While no significant difference in ERK1/2 and JNK MAPKs in zebrafish was observed, P38 phosphorylation was significantly decreased at 7 and 14 d, and the changes in MAPKs were more evident in EPC cells previously exposed to AZ at 7 d. These results revealed that AZ initially induced low phosphorylation of MAPKs, triggering the attenuation of Nrf2 phosphorylation to weaken Nrf2 translocation into the nucleus in a longer exposure period (more than 5 d). The data in the cells and fish also showed that antioxidant activities were decreased to some extent at 5–7 d for the cells and 7–14 d for the fish. Furthermore, interferon-related factors were decreased in AZ-exposed zebrafish, explaining the reason that fish can't resist the virus infection. Overall, the present study provided a new adverse threat of AZ by amplifying the viral outbreak to endanger ecological safety in aquatic environment.
研究领域:
环境科学
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鱼类病理学
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毒理学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2017
·
IF 5.099
Tao Lu,Youchao Zhu,Jiahui Xu,Mingjing Ke,Meng Zhang,Chengxia Tan,Zhengwei Fu,Haifeng Qian
The top-selling strobilurin, azoxystrobin (AZ), is a broad-spectrum fungicide that protects against many kinds of pathogenic fungi by preventing their ATP production. The extensive use of AZ can have negative consequences on non-target species and its effects and toxic mechanisms on algae are still poorly understood. In this work, Chlorella pyrenoidosa that had been grown in BG-11 medium was exposed to AZ (0.5–10 mg L −1 ) for 10 d. The physiological and molecular responses of the algae to AZ treatment, including photosynthetic efficiency, lipid peroxidation level, antioxidant enzyme activities , as well as transcriptome-based analysis of gene expression, were examined to investigate the potential toxic mechanism. Results shows that the photosynthetic pigment (per cell) increased slightly after AZ treatments, indicating that the photosystem of C. pyrenoidosa may have been strengthened. Glutathione and ascorbate contents were increased, and antioxidant enzyme activities were induced to relieve oxidative damage (e.g., from lipid peroxidation) in algae after AZ treatment. Transcriptome-based analysis of gene expression combined with physiological verification suggested that the 5 mg L −1 AZ treatment did not inhibit ATP generation in C. pyrenoidosa , but did significantly alter amino acid metabolism, especially in aspartate- and glutamine-related reactions. Moreover, perturbation of ascorbate synthesis, fat acid metabolism, and RNA translation was also observed, suggesting that AZ inhibits algal cell growth through multiple pathways. The identification of AZ-responsive genes in the eukaryotic alga C. pyrenoidosa provides new insight into AZ stress responses in a non-target organism.
研究领域:
环境科学
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毒理学
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植物生理学
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产品关联
DOI
PubMed
高影响因子
Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2019
·
IF 1.163
Xinxin Liu,Yan Wang,Ling Li,Ranhong Li
In this research, hydrophilic molecularly imprinted microspheres (HMIPs) for azoxystrobin were successfully synthesized through precipitation polymerization. The adsorption capacities of HMIPs for azoxystrobin in water medium were higher than ordinary molecularly imprinted microspheres (MIPs), and HMIPs exhibited good hydrophilic properties. HMIPs and non-imprinted microspheres (HNIPs) were characterized by FT-IR, SEM, laser particle size analyzer and TGA. Comparing with HNIPs, azoxystrobin had a significant influence on morphologys and sizes of HMIPs. The Langmuir adsorption isotherm illustrated each binding site of HMIPs had the same adsorption capacity. The Lagergran pseudo-second-order kinetic model indicated the adsorption process between azoxystrobin and HMIPs was chemical adsorption. BET test illustrated HMIPs had bigger specific surface areas than HNIPs. Selective adsorption indicated that HMIPs had highly specific recognition of azoxystrobin. HMIPs successfully exhibited high selectivity and high hydrophilicity in water medium.
研究领域:
环境化学
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高分子科学
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分析化学
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分子印迹技术
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
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IF 3.974
Benben Du,Zhenyan Zhang,Wanyue Liu,Yizhi Ye,Tao Lu,Zhigao Zhou,Yan Li,Zhanyu Fu,Haifeng Qian
Azoxystrobin (AZ) is an effective broad-spectrum fungicide . Due to its extensive application, AZ is detectable in aquatic ecosystems and thus influences aquatic organisms. In this study, the acute toxicity (96 h) of AZ at concentrations of 1.0 mg/L and 5.0 mg/L on the diatom Phaeodactylum tricornutum were examined. At the tested concentrations, AZ significantly inhibited P. tricornutum growth and destroyed its cellular structure. Furthermore, the mechanisms of AZ-induced toxicity on P. tricornutum changed as the exposure time extended. Forty-eight hours after exposure, AZ inhibited P. tricornutum growth primarily via inducing oxidative stress , which increased the activity of two main antioxidant enzymes, superoxide dismutase and peroxidase, and inhibited energy metabolism. However, after 96 h of treatment, the decline in the photosynthetic capacity of P. tricornutum demonstrated that the photosystem was the main AZ target. The pigment content and expression levels of genes related to photosynthetic electron transfer reactions were also significantly decreased. The present study describes AZ toxicity in P. tricornutum and is very valuable for assessing the environmental risk of AZ.
研究领域:
环境科学
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毒理学
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水生生态学
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
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2020
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IF 4.302
Mengwei Zhang,Wanyue Liu,Qian Qu,Mingjing Ke,Zhenyan Zhang,Zhigao Zhou,Tao Lu,Haifeng Qian
An effective broad-spectrum fungicide , azoxystrobin (AZ), has been widely detected in aquatic ecosystems , potentially affecting the growth of aquatic microorganisms . In the present study, the eukaryotic alga Monoraphidium sp. and the cyanobacterium Pseudanabaena sp. were exposed to AZ for 7 days. Our results showed that 0.2–0.5 mg/L concentrations of AZ slightly inhibited the growth of Monoraphidium sp. but stimulated Pseudanabaena sp. growth. Meanwhile, AZ treatment effectively increased the secretion of total organic carbon (TOC) in the culture media of the two species, and this phenomenon was also found in a freshwater microcosm experiment (containing the natural microbial community). We attempted to assess the effect of AZ on the function of aquatic microbial communities through metabolomic analysis and further explore the potential risks of this compound. The metabonomic profiles of the microcosm indicated that the most varied metabolites after AZ treatment were related to the citrate cycle (TCA), fatty acid biosynthesis and purine metabolism. We thereby inferred that the microbial community increased extracellular secretions by adjusting metabolic pathways, which might be a stress response to reduce AZ toxicity. Our results provide an important theoretical basis for further study of fungicide stress responses in aquatic microcosm microbial communities, as well as a good start for further explorations of AZ detoxification mechanisms, which will be valuable for the evaluation of AZ environmental risk.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2019
·Vol. 103
·期 5
·683-688
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IF 1.65
Lu Tao,Zhou Zhigao,Zhang Qi,Zhang Zhenyan,Qian Haifeng
Extensive use of the fungicides azoxystrobin (AZ) and pyraclostrobin (PYR) can have negative effects on aquatic environments, but comprehensive studies on their effect on aquatic microbial communities are still lacking. We found that AZ and PYR could both inhibit the growth of Chlorella vulgaris , but PYR also inhibited Microcystis aeruginosa more strongly than did AZ. High-throughput sequencing analysis showed that AZ promoted the growth of Cyanobacteria in microcosms, and both PYR and AZ disturbed the ecological balance in the aquatic bacterial community and created distinct ecological risks. Our study suggests that the ecological risk of fungicides is complex, and fungicide use should be better managed to reduce potential risks to the environment.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Hagar M.S. Salman,Tianyang You,Jie Zhu,Mohamed Kahiel,Minghua Wang,Jinsheng Duan,Xiude Hua
Azoxystrobin (AZX) is a widely used fungicide for the prevention and management of agricultural diseases, but its adverse effects on non-target organisms have raised concerns. In this study, monoclonal antibodies (mAbs) against AZX were developed to establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold immunochromatographic assay (GICA) for AZX detection. Eight hybridoma cell lines capable of stably secreting anti-AZX mAbs were prepared, among which the mAb 2A3C6 with the highest sensitivity was used in the immunoassays. The ic-ELISA showed a half-maximal inhibitory concentration (IC 50 ) of 0.61 μg L −1 , and the GICA demonstrated a visual limit of detection (vLOD) of 0.6 μg L −1 . Both assays demonstrated high specificity, with no cross-reactivity observed for AZX analogues. Spiked recovery and blind sample detection confirmed the excellent sensitivity and reliability of these immunoassays for detecting AZX in environmental and agricultural samples.
研究领域:
农业化学
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免疫学
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环境科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD PROTECTION
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2017
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IF 1.417
Dan Hu,Xu Xu,Tian Cai,Wei-Ying Wang,Chun-Jie Wu,Li-Ming Ye
ABSTRACT A rapid and sensitive analytical method based on high-performance liquid chromatography–tandem mass spectrometry was developed and validated for the determination of isopyrazam (IZM) and azoxystrobin (AZT) in cucumbers. A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method was used as the pretreatment procedure. The samples were extracted with acetonitrile and cleaned up with octadecylsilyl silica (C18) and graphite carbon black. The proposed method resulted in satisfactory recovery of IZM and AZT (91.48 to 114.62%), and relative standard deviations were less than 13.1% at fortification concentrations of 1, 20, and 500 μg kg −1 ( n = 3). The limits of quantification for IZM and AZT were 0.498 and 0.499 μg kg −1 , respectively, which are far below the maximum residue level (0.5 mg kg −1 ) established for this type of sample. Matrix effects were also evaluated. This study established a sensitive and fast method for the detection of IZM and AZT in cucumber samples.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2023
·
IF 7.3
Huanbin Shi,Hui Wen,Shuwei Xie,Yuan Li,Ya Chen,Zhiquan Liu,Nan Jiang,Jiehua Qiu,Xueming Zhu,Fucheng Lin,Yanjun Kou
Silver nanoparticles (AgNPs) exhibit a broad spectrum of antimicrobial activity against various fungal pathogens, including the devastating rice blast fungus Magnaporthe oryzae, which threatens rice production worldwide. However, the antifungal molecular mechanisms of AgNPs against M. oryzae are still poorly understood. Here, it was found that the mycelial growth and virulence of M. oryzae strains were significantly reduced by AgNPs. Two nm-AgNPs at an EC50 concentration of 2.0 μg mL−1 almost impaired 100% virulence of M. oryzae. Further studies revealed that AgNP treatment changes cell wall morphology and suppresses appressorium formation. The defects in appressorium development upon AgNP treatment were caused by the reduced phosphorylation level of the MAPK MoPmk1 and impaired conidial autophagy. Importantly, the combination of AgNPs and azoxystrobin at appropriate ratios enhanced fungitoxicity to azoxystrobin-sensitive/resistant M. oryzae strains. Due to the excellent control effect of AgNPs on rice blast, we established a method for the biosynthesis of AgNPs by using the biocontrol Bacillus strain Tu27 to reduce waste environmental pollution and synthesis costs. Infection assays showed that the biosynthesized AgNPs displayed similar fungal toxicity. Taken together, our results revealed that AgNPs display the antifungal activity by a unique mechanism of damaging cell wall integrity, reducing phosphorylation of the MAPK MoPmk1, and impairing autophagy, and have an additive synergy with azoxystrobin on M. oryzae.
研究领域:
植物病理学
·
分子生物学
·
纳米技术
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农业科学
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DOI
高影响因子
EUROPEAN JOURNAL OF PLANT PATHOLOGY
·
2023
·
IF 1.8
Xiang Ligang,Yu Zhihe,Wang Hancheng,Cai Liuti,Hsiang Tom,Li Wenhong,Guo Moyan,Li Tong,Sun Meili
Tobacco, as one of the major cash crops in Guizhou province, China, is severely affected by diseases caused by Rhizoctonia solani . Azoxystrobin is a quinone outside inhibitor (QoI) fungicide extensively used to control various crop diseases in China. To evaluate the potential effect of azoxystrobin on the physiology, morphology, and sclerotial formation of Rhizoctonia solani , 104 isolates of R. solani from tobacco collected in Guizhou were evaluated for in vitro sensitivity to azoxystrobin, and scanning electron microscopy was used to observe the effects of azoxystrobin on mycelial morphology. The effects of azoxystrobin on reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), and succinate dehydrogenase (SDH) in hyphae at three time points (at 5, 10 and 15 days) and on sclerotial differentiation were examined. The effective concentration that caused 50% growth inhibition (EC 50 ) ranged from 0.3 to 552 μg/mL with a geometric mean of 8.4 μg/mL, and the frequency distribution of EC 50 values was unimodal. Scanning electron microscopy revealed that azoxystrobin caused folding, collapse and crumpling of hyphae. Under azoxystrobin stress, sclerotial differentiation was inhibited, and the production of hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2− ) as well as activities of SOD, CAT and SDH in hyphae increased significantly compared to the untreated control at day 5; while at day 10 or 15, H 2 O 2 production decreased significantly, CAT activity increased significantly, and O 2− , SOD, and SDH concentrations returned to normal levels. In general, the results provide a better understanding of the physiological effects of azoxystrobin on R. solani .
研究领域:
植物病理学
·
真菌生物学
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农业科学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Zhiyuan Meng,Qinchao Wu,Xinyi Wu,Chunmei Yang,Wangjin Xu,Tingting Lin,You Liang,Xiaojun Chen
The enhancement of pesticide utilization efficiency for improved biological activity, while promoting crop growth to enhance disease resistance, holds significant importance in agricultural production. In this study, we developed an enzyme-responsive azoxystrobin (AZOX) delivery system using iron-based mesoporous materials as porous carriers and pectin (Pec) as an encapsulation agent to manage rice sheath blight. The resultant AZOX-AFS-Pec nanoparticles (NPs) exhibit high loading capacity and could intelligently release AZOX in acidic conditions in the presence of pectinase. Notably, AZOX-AFS-Pec NPs demonstrated improved wetting and adhesion properties on rice blades, thereby augmenting AZOX utilization. Furthermore, AZOX-AFS-Pec NPs enhanced fungicidal activity against rice sheath blight, with pot experiments revealing comparable control efficacy on rice sheath blight to AZOX suspension concentration (SC). Specifically, AZOX-AFS-Pec NPs exhibited superior prevention and prolonged protection against rice sheath blight compared to AZOX SC. Additionally, continuous release of Fe2+ and Fe3+ by AZOX-AFS-Pec NPs increased chlorophyll content and promoted rice growth. Moreover, these NPs increased salicylic acid (SA) levels in rice plants, fortifying disease resistance. Importantly, AZOX-AFS-Pec NPs demonstrated over a 2-fold reduction in acute toxicity to earthworms compared to AZOX SC. Therefore, stimuli-responsive AZOX-AFS-Pec NPs show great promise for enhancing pesticide effectiveness against pathogens, improving crop traits, and fortifying disease resistance.
研究领域:
农业科学
·
植物病理学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）