Well-defined compositional assemblies of plant-based yogurt are of fast-growing awareness for world population concerning environmental sustainability, economic burdens and health risks. Soybean is an attractive candidate for plant yogurt, suffering from poor flavor, limited nutrition, and undesired allergens to offer healthy-functional segments. Herein, we deciphered a novel lycopene-soy yogurt by efficient two-stage fermentation of engineered B. subtilis and LAB. The fortified sogurt was ensured with redundant lycopene of 22.67 ± 2.95 mg/g DCW by engineered B. subtilis and enriched soy isoflavone from synergistic effects of engineered B. subtilis and LAB, possessing strong antioxidant capacity for upgrading functionality. Moreover, the desired pH, accelerated protein hydrolysis, enhanced amino acid availability, and expected sensory attributes cooperatively conferred lycopene-soy yogurt as healthy functional food. High potential is firstly ascribed to sequential dual culture of engineered B. subtilis and LAB in lycopene-soy yogurt, in which flavorful, hypoallergenic and antioxidative ingredients enabled functionalities for plant-based yogurt.
Lei Zhang,Yong Shi,Qinyu Xu,Jingbo Yu,Qingya Li,Lirong Huang,Xinxin Kang,Shujun Wang,Jie Qiao
Although a protective shell based on a metal-organic framework (MOF) can effectively improve the remediation of polycyclic aromatic hydrocarbons (PAHs) by microorganisms, the functional characteristics of the shell are often limited by the surface charge, chemical properties and intrinsic strain characteristics. This study explored the potential of mixed organic acids (MA) as an eco-friendly modifier for zeolite imidazolate framework-8 (ZIF-8), facilitating the formation of a biomimetic mineralized shell around Bacillus subtilis ZL09-26. The addition of MA into the ZIF-8 protective shell was found to stimulate growth and bolster cell viability. Notably, adding MA contribute resulted in a 1.98- fold enhancement of the PHE degradation efficiency. After five cycles of reuse, ZIF-8-MA@ B. subtilis ZL09-26 still maintained almost 90 % of the initial PHE degradation ability. Proteomic analysis revealed a coordinated regulation of multiple metabolic pathways that facilitated PHE uptake and degradation, including central carbon metabolism, direct PHE biodegradation, oxidative phosphorylation, aminoacyl-tRNA biosynthesis, fatty acid biosynthesis, ABC transporters, and the biosynthesis of valine, leucine, isoleucine, and lysine. This work broadens the application potential of biomineralized microorganisms, providing novel strategies for the sustainable bioremediation of xenobiotic pollutants in the environment.
The objective of this study was to isolate and characterize antioxidant peptides from mulberry leaves fermented with Bacillus subtilis H4 and Bacillus amyloliquefaciens LFB112. The results indicated that fraction F4 (<1 kDa) exhibited superior DPPH activity compared to the F1 (>10 kDa), F2 (3–10 kDa), and F3 (1–3 kDa) fractions, and cytoprotective effect against lipopolysaccharides (LPS)-induced oxidative stress in RAW264.7 cells. Three novel peptides, FRFDP, RFGG, and GPPLAFGGGP, were identified in the F4 fraction of the mulberry leaf ferment, and docking results showed that these peptides could form stable carbon (covalent) and hydrogen bonds to the active sites of Keap1, thus regulating the Keap1-Nrf2 pathway by blocking the Nrf2 binding sites on Keap1. These peptides significantly upregulated the mRNA expression of Nrf2 , HO-1 , and NQO1 , providing indirect evidence of their potential to enhance cellular antioxidant defense via the possible activation of Keap1-Nrf2 pathway. Furthermore, these peptides showed good DPPH and ABTS scavenging activities, indicating their potential as antioxidant peptides. This study offers valuable insights into the integration of these novel peptides in developing mulberry leaf ferment as a functional food and their potential use as a feed additive.
危险品化学品经营许可证（带存储）