TALANTA
·
2024
·
IF 5.6
Hang Chen,Xuelin Dong,Kaide Ou,Xin Cong,Yonggui Liao,Yajiang Yang,Hong Wang
Sensing biogenic amine (BAs) content is very important for assessing food freshness. To address the limitations such as small color difference values (ΔE) and complex preparation of probes for visualizing the freshness of seafood, a pH-responsive ratiometric fluorescent probe (EnEB) was prepared by Eu(NO 3 ) 3 , trimeric acid (BTC), and hydrochloric acid norepinephrine (Enr). EnEB emitted blue (446 nm) and red fluorescence (616 nm) originating from Enr and Eu 3+ , respectively, and exhibiting a fluorescence wavelength difference up to 170 nm. The ratiometric fluorescent signals of EnEB showed a linear correlation with pH in the range of 5.5–8.0. Thus, EnEB can rapidly and precisely detect BAs, such as histamine, tyramine, and spermine, with detection limits and response times of 1.14 μmol/L (3 s), 1.04 μmol/L (8 s), and 0.41 μmol/L (2 s), respectively. Furthermore, an EnEB aerogel was prepared by loading EnEB in a matrix formed by polyvinyl alcohol (PVA) and agarose (AG). EnEB aerogel exhibited excellent acid-base gas-sensing properties. The fluorescence color of EnEB aerogel can change significantly with the deterioration of seafood. When seafood changed from fresh to decayed, the ΔE value of EnEB aerogel was as high as 80.9. Importantly, the results of seafood freshness by naked eye using EnEB aerogel was consistent well with the TVB-N content and the freshness standard stipulated by national food standard, indicating EnEB aerogel can accurately visually and real-time monitor seafood freshness. Furthermore, the strategy for sensing food freshness based on EnEB aerogel also offered multiple color variations to indicate fine freshness levels of seafood. This work provided a convenient, efficient, and accurate approach to assessing the freshness of seafood. Additionally, EnEB also has promising applications in security and anti-counterfeiting.
研究领域:
食品科学
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分析化学
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材料科学
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传感器
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 9
·3715-3725
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IF 3.67
Wang Chengcheng,Zheng Pu,Chen Pengcheng
Raspberry ketone is an important ingredient in the flavor and fragrance industries. Due to its low content in fruits and vegetables, the production of natural raspberry ketone using heterologous synthesis in microbial strains is recently attracting increased attention. In this work, a heterologous pathway to produce raspberry ketone from p -coumaric acid, including 4-coumarate: CoA ligase (4CL), benzalacetone synthase (BAS), and raspberry ketone/zingerone synthase (RZS1) from plants, was successfully assembled in Escherichia coli . When the RZS1 gene was introduced into E. coli and co-expressed with two other genes, the intermediate 4-hydroxybenzylidene acetone in the pathway was almost completely transformed into a raspberry ketone. Substituting TB medium for M9 medium increased raspberry ketone titers by 3–4 times. Furthermore, the heterologous pathway was partitioned into two modules; module one produced p -coumaroyl-CoA from p -coumaric acid by 4CL, and module two produced raspberry ketone from coumaroyl-CoA by the action of BAS and RZS1. Optimizing the balanced expression of the two modules, it was shown that moderate expression of module one and high expression of module two was the best combination to enhance raspberry ketone production. The engineered strain CZ-8 reached 90.97 mg/l of raspberry ketone, which was 12 times higher than previously reported. In addition, the preferred approach of the heterologous pathway was related to the heterologous genes from different sources; for example, 4CL from Arabidopsis thaliana seemed to be more suitable for raspberry ketone production than that from Petroselinum crispum . This work paves an alternative way for future economic production of natural raspberry ketone.
研究领域:
生物化学
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合成生物学
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2019
·
IF 4.029
Qian Li,Zhihua Yang,Xingqi Liao,Daxin Li,Delong Cai,Bo Niu,Peigang He,Xiaoming Duan,Dechang Jia,Yu Zhou
This study aims to elucidate the phase transformation of hexacelsian-to-celsian in 10 wt.% h -BN reinforced BaAl 2 Si 2 O 8 (BN/BAS) composites fabricated by hot-pressed at 1300 °C for different holding time. Results showed that the relationship between the transformation fraction of celsian and the holding time displays a typical “S” curve according to Johnson-Mehl-Avrami equation. During the hexacelsian-to-celsian transformation, a transitional phase of paracelsian was observed. Celsian tended to nucleate and grow up along the (110) P habit plane of paracelsian by a non-diffusive transition behavior. During transformation, celsian and paracelsian present an oriented intergrowth with orientation relations of (110) P //(020) M , [001] P //[101] M and [ 1 1 ¯ 0 ] P // [ 10 1 ¯ ] M .
研究领域:
材料科学
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无机化学
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DOI
高影响因子
CERAMICS INTERNATIONAL
·
2026
·
IF 6
Qian Li,Zhenwei Yang,Yangyang Feng,Daxin Li,Zhaoxue Deng,Su Cheng
(SiC-BN)/BAS composite ceramics were prepared via reactive hot-press sintering at 1500 °C/10 MPa for 1 h. The effects of BN contents (0-14 wt.%) on the phase composition, density, microstructure, and mechanical properties of the composites were systematically investigated. The research showed that the incorporation of coal fly ash (CFA) and BN promoted a partial hexacelsian-to-celsian transformation. The BAS in composite ceramics primarily consists of hexacelsian and minor celsian. The celsian content reaches a maximum at 7 wt.% BN, corresponding to the peak residual compressive stress. The addition of BN leads to a gradual decrease in density and flexural strength, while fracture toughness first increases and then declines. At 7 wt.% BN, the ceramics achieve optimal comprehensive performance, with flexural strength and fracture toughness reaching 194.9 MPa and 3.74 MPa· m 1/2 , respectively. The enhancement is mainly attributed to the synergistic effects of weak interfaces introduced by BN (platelet pull-out, crack deflection, and crack bridging) and residual compressive stress induced by thermal expansion mismatch among the constituent phases.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Li Xia,Zurong Shi,Guangxin Ren,Wenya Wei,Mingjuan Zhang,Yue Wang,Yun Wang
Ethylene vinyl alcohol-bayberry anthocyanins-carvacrol (EVOH-BAs-CA) membrane is developed for fruit freshness monitoring and maintaining. • BAs endow the membrane antioxidant activity. • Antibacterial activity of the membrane is from the control released CA. • EVOH-BAs-CA membrane has a reversible transparency response to ethanol exudate. In this study, a visual freshness indicator based on electrospun fibers membrane was fabricated, which relies on ethanol-responsive light transmittance to evaluate fruit freshness. Carvacrol (CA) and bayberry anthocyanins (BAs) were incorporated into ethylene-vinyl alcohol (EVOH) matrix to prepare the composite membrane for bayberry freshness monitoring and preservation. The EVOH-BAs-CA membrane was initially opaque and turned transparent, as ethanol (EtOH) exudates released from spoiled bayberry induced a rapid increase in light transmittance during storage. The membrane showed high sensitivity to ethanol exudates, retaining a transmittance above 60% after 10 reuse cycles. Additionally, the EVOH-BAs-CA membrane exhibits strong antibacterial and antioxidant properties and can approximately prolong the freshness of bayberry for 4 days, which resulted from the effective release of CA and BAs. The implementation of the EVOH-BAs-CA membrane in visual freshness monitoring for bayberry highlights its significant potential within intelligent packaging systems. Download: Download high-res image (127KB) Download: Download full-size image
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Li Xia,Linlin Li,Yewen Xiao,Feng Xiao,Lanhua Liu,Xiangying Chen,Xingjiang Li,Hualin Wang
Currently, designing smart membranes with multifunctional effectiveness is crucial to food freshness monitoring and retention. Herein, an active colorimetric Janus bilayer membrane with directional water transport (DWT) performance is constructed by electrospinning, which comprises a hydrophilic layer of silk fibroin-bayberry anthocyanins (SF-BAs) and a hydrophobic layer of polycaprolactone-eucalyptus oil (PCL-EO). The entities of BAs and EO are well dispersed in the fiber matrix by hydrogen bonds and physical interactions, respectively. BAs endow the membrane colorimetric response and antioxidant activity, and EO contributes to the antibacterial activity while DWT performance is generated from the asymmetric wettability of the two layers. The bilayer membrane has an accumulative one-way transport index of 1077%, an overall moisture management capacity of 0.76 and a water evaporation rate of 0.48 g h −1 . Moreover, the release of BAs and EO was predominantly controlled by Fickian diffusion. As a pH-sensing indicator, PCL-EO@SF-BAs is highly sensitive to external pH stimuli and the response is reversible. In addition to freshness monitoring, PCL-EO@SF-BAs can extend the shelf-life of pork beyond 100% at 4 °C. Also, it can extend the shelf life of shrimp by approximately 70% at 25 °C with the synergistic effect of antibacterial activity and the DWT performance.
研究领域:
食品科学
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材料科学
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高影响因子
POLYMER BULLETIN
·
2017
·Vol. 74
·期 12
·5231-5247
·
IF 1.43
Yang Dongcheng,Yu Lili,Chen Hou,Yu Yanbo,Xu Yuanyuan,Sun Jinming,Wang Yating
Bromoactylated apple seeds (BAS) was used as new source for the synthesis of environmental adsorbent. Apple seeds were modified by bromoacetyl bromide to obtain a macroinitiator for single electron transfer-living radical polymerization (SET-LRP) of acrylonitrile (AN). The amidoxime (AO) adsorbent was prepared by the reaction of the graft copolymer bromoactylated apple seeds (BAS)/polyacrylonitrile (BAS- g -PAN) with hydroxylamine. Amidoxime BAS- g -PAN (AO BAS- g -PAN) was used to remove Pb(II) and the maximum adsorption for Pb(II) was 1.58 mmol/g. AO BAS- g -PAN could also selectively adsorb Pb(II) from binary ion systems in the existence of Ag(I), Ni(II) and Zn(II). This efficient modifying approach provides a new powerful mean toward filling the gap on apple waste as a potential source for controlling heavy metal pollution.
研究领域:
环境科学
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高分子化学
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废物管理
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Enming Miao,Nazhen Zhang,Shiwei Lu,Yufeng Hu,Lin Fu,Hao Zhou,Jingjing Zhan,Minghuo Wu
A simple magnetization method was developed for the preparation of magnetic materials from conventional solid phase packing though coprecipitation and solvothermal approaches. And the prepared magnetic materials were used for magnetic solid phase extraction (MSPE) of biogenic amines (BAs) from beers. Furthermore, to improve the analytical throughput, a solid phase “on-situ” quadraplex isotope dimethyl labeling method was developed for the quantification of BAs by liquid chromatography-high resolution mass spectrometry (LC HRMS). Compared to conventional in-solution labeling, the “on-situ” labeling could simplify the sample preparation procedure and efficiently remove the residuals such as inorganic salts and excessive labeling reagents. The quadraplex labeling, which enabled three real samples and one internal standard sample to be analyzed simultaneously in a single LC HRMS run. For the tested 8 BAs (cadaverine, phenethylamine, spermine, spermidine, tyramine, histamine, putrescine and tryptamine), LODs of 0.02–0.05 μg/L and LOQs of 0.05–0.1 μg/L were achieved at good reproducibility (RSD of 0.5–4.6% and 2.2–7.0% for intra- and inter-day reproducibility, respectively). With this method, six beer samples were analyzed, and these 8 BAs were all detected in the range of low μg/L to 2.9 mg/L, which were lower than maximal residual level (MRL) required in the regulations of China and EU.
研究领域:
分析化学
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食品科学
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质谱分析
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高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Chuyao Liao,Di Wang,Siyuan Qin,Ying Zhang,Jie Chen,Ruijie Xu,Fengguo Xu,Pei Zhang
Irinotecan (CPT-11) in combination with 5-fluorouracil and leucovorin is a first-line chemotherapy regimen for the treatment of colorectal cancer; however, its clinical application is limited by the dose-limiting gastrointestinal toxicity of colitis. In our previous studies, several bile acids (BAs) were found significantly elevated in the colon of the CPT-11-induced rat colitis model. On the other hand, NLRP3 inflammasome has been reported to play important roles in mediating colitis. Interestingly, BA was stated to activate the NLRP3 inflammasome in some studies, while in some other reports, it showed an inhibitory effect. We assumed that the inflammatory status in different circumstances might have contributed to the controversial findings. In this study, we first discovered, under non-inflammatory conditions, that supplementing BA could activate the NLRP3 inflammasome in THP-1-differentiated macrophages and promote inflammation. In lipopolysaccharide (LPS)-induced inflammatory macrophages, however, BA inhibited the NLRP3 inflammasome and reduced inflammation. Further experiments demonstrated that Takeda G protein-coupled receptor 5 (TGR5) is essential in mediating the inhibitory effect of BA, while phospho-SP1 (p-SP1) is key to the activation. Furthermore, we applied the above findings to ameliorate CPT-11-caused colitis in rats by inhibiting SP1 with mithramycin A (MitA) or activating TGR5 using oleanolic acid (OA). Our findings may shed light on the discovery of effective interventions for reducing dose-limiting chemotherapy-induced colitis.
研究领域:
肿瘤学
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胃肠病学
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免疫学
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药理学
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高影响因子
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