JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Xinyu Yao,Jinying Gao,Lanqiao Wang,Xiaoning Hou,Litao Ge,Xinxin Qin,Jiazhang Qiu,Xuming Deng,Wei Li,Jianfeng Wang
Ethnopharmacology relevance Cananga oil (CO) is derived from the flowers of the traditional medicinal plant, the ylang-ylang tree. As a traditional antidepressant, CO is commonly utilized in the treatment of various mental disorders including depression, anxiety, and autism. It is also recognized as an efficient antibacterial insecticide, and has been traditionally utilized to combat malaria and acute inflammatory responses resulting from bacterial infections both in vitro and in vivo . Aim of the study The objective of this study is to comprehensively investigate the anti- Salmonella activity and mechanism of CO both in vitro and in vivo , with the expectation of providing feasible strategies for exploring new antimicrobial strategies and developing novel drugs. Methods The in vitro antibacterial activity of CO was comprehensively analyzed by measuring MIC, MBC, growth curve, time-killing curve, surface motility, biofilm, and Live/dead bacterial staining. The analysis of the chemistry and active ingredients of CO was conducted using GC-MS. To examine the influence of CO on the membrane homeostasis of Salmonella , we conducted utilizing diverse techniques, including ANS, PI, NPN, ONPG, BCECF-AM, DiSC3(5), and scanning electron microscopy (SEM) analysis. In addition, the antibacterial mechanism of CO was analyzed and validated through metabolomics analysis. Finally, a mouse infection model of Salmonella typhimurium was established to evaluate the toxic side effects and therapeutic effects of CO. Results The antibacterial effect of CO is the result of the combined action of the main chemical components within its six (palmitic acid, α-linolenic acid, stearic acid, benzyl benzoate, benzyl acetate, and myristic acid). Furthermore, CO disrupts the balance of purine metabolism and the tricarboxylic acid cycle (TCA cycle) in Salmonella , interfering with redox processes. This leads to energy metabolic disorders and oxidative stress damage within the bacteria, resulting in bacterial shock, enhanced membrane damage, and ultimately bacterial death. It is worth emphasizing that CO exerts an effective protective influence on Salmonella infection in vivo within a non-toxic concentration range. Conclusion The outcomes indicate that CO displays remarkable anti- Salmonella activity both in vitro and in vivo . It triggers bacterial death by disrupting the balance of purine metabolism and the TCA cycle, interfering with the redox process, making it a promising anti- Salmonella medication.
研究领域:
药理学
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微生物学
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代谢组学
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传染病学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Cellular and Infection Microbiology
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2026
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IF 5.5
Yujuan Li,Yi Yang,Ting Zhou,Jing Zhou,Jie Hu,Shuangshuang Liang,Zhenhua Xie,Qing Cheng,Yachao Jiang,Chao Jiang,Qing Liu
Amid the growing antibiotic resistance crisis, colistin remains a last-line therapeutic option for multidrug-resistant (MDR) Gram-negative bacterial infections. However, the emergence and rapid dissemination of plasmid-mediated colistin resistance gene (mcr) have markedly reduced its clinical effectiveness. Utilizing colistin adjuvants to restore its antibacterial potency is a promising strategy to combat this threat. In this study, we aimed to investigate the synergistic effects of α-terpineol in combination with colistin against colistin-resistant MDR Escherichia coli (E. coli) and to elucidate the underlying mechanisms of this synergy. In vitro synergistic activity was evaluated using checkerboard microdilution assays, time-kill curve analyses, and resistance development studies. Mechanistic insights were obtained through transcriptomic analysis and fluorescence-probe based assays. In vivo efficacy was validated using Galleria mellonella and mouse acute peritonitis infection models. The results showed that the combination of α-terpineol and colistin exhibited synergistic bactericidal activity and suppressed the development of colistin resistance. α-Terpineol increased inner- and outer-membrane fluidity in E. coli, thereby enhancing colistin uptake. Furthermore, it inhibited superoxide dismutase (SOD) activity, preventing the conversion of O2·– to H2O2 and causing superoxide accumulation. Elevated O2·– levels damaged iron–sulfur clusters and impaired respiratory function. Together with the proton motive force (PMF) dissipation, these effects severely impaired ATP synthesis, ultimately sensitizing MDR E. coli to colistin. The combination also showed significant therapeutic efficacy in animal models. Our findings identify α-terpineol as a promising colistin adjuvant that restores antibacterial activity by disrupting membrane integrity and impairing antioxidant defenses, offering a viable strategy for treating MDR Gram-negative bacterial infections.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2025
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IF 5.9
Liang Peng,Chao Ma,Xiujuan Huang,Zhang Minrui,Xueni Huang,Lining Qi,Pimin Gong
Dormancy can enhance the spray-drying resistance of lactic acid bacteria(LAB), but it can also prolong their revival time during fermentation. There was a close relationship between dormancy and intracellular protein aggregation, and pH was an important factor that affects protein aggregation. This study innovatively elucidates the molecular mechanism by which extracellular pH coordinates the dormancy-metabolism balance in Lactobacillus bulgaricus ( L. bulgaricus )through regulation of intracellular protein aggregation dynamics. This study revealed that subtle environmental pH variations can induce significant reversible transitions in protein aggregation states, exhibiting remarkable pH sensitivity (with a 40-fold difference in solubility between pH 6.0-6.5) and directly influencing industrial performance characteristics. Significantly, quantitative relationships between pH-regulated protein aggregation levels and both cellular stress resistance and metabolic activity were established, thus providing theoretical foundations and practical guidance for developing high-viability probiotic preparations. Furthermore, this study integrated environmental factor regulation, protein conformational changes, and microbial industrial performance into a unified framework, opening new avenues for the industrial application of probiotics.
研究领域:
微生物学
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生物技术
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食品科学
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分子生物学
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生物物理学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）