Nanotoxicology
·
2021
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IF 5.913
Chao Wang,Jiansheng Zhu,Xing Gong,Yinyin Liang,Shuyu Xu,Yongquan Yu,Liu Yang,Jiayi Xu,Shou-Lin Wang
This study attempts to explore the potential impact of titanium dioxide nanoparticles (n-TiO2) on bioconcentration and reproductive impairments of male zebrafish in the presence of 2,2′,4,4′-tetrabromodiphenyl ether (BDE47), the congener of PBDEs predominant in environment and most abundant in biosamples. n-TiO2 nanoparticles strongly adsorbed BDE47 to form BDE47/TiO2 complex, which was taken up into the testes of zebrafish, and increased the tissue burdens of both BDE47 and n-TiO2. Correspondingly, no observed toxic dose of n-TiO2 (100 μg/L) was found to aggravate the abnormal histological morphology of the testes and the decrease in egg production, gonadosomatic index, sexual hormone levels and related gene expression in zebrafish in the presence of BDE47 at 5 or 50 μg/L. In addition, n-TiO2 exacerbated the destruction resulting from the ultrastructural disassembly of intercellular connectivity of germ cells in zebrafish and the decrease in transepithelial electrical resistance in TM4 cells induced by BDE47. Furthermore, n-TiO2 enhanced BDE47 to initially activate p-JNK MAPK signaling pathway and subsequently triggered the downregulation of junction proteins (i.e., ZO-1, Connexin-43 and N-cadherin), leading to impaired cell-cell junctions in vivo and in vitro. Our results demonstrated that n-TiO2 should act as a carrier to facilitate the accumulation of BDE47 in zebrafish testes and result in a synergistic effect on BDE47-induced adverse reproductive outcomes via disruption of intercellular connectivity of zebrafish testes. This study is beneficial in providing a scientific basis for improving the health risk assessment of environmental pollutants, particularly those that coexist with nanoparticle contamination in realistic environments.
研究领域:
环境毒理学
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生殖生物学
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纳米技术
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Cheng-Qiang Wang,Zou Su,Chun-Guang Dai,Jia-Le Song,Bo Qian
2,2′,4,4′-tetrabromodiphenyl ether (BDE47) is a foodborne environmental risk factor for depression, but the pathogenic mechanism has yet to be fully characterized. In this study, we clarified the effect of BDE47 on depression in mice. The abnormal regulation of the microbiome-gut-brain axis is evidenced closely associated with the development of depression. Using RNA sequencing, metabolomics, and 16s rDNA amplicon sequencing, the role of the microbiome-gut-brain axis in depression was also explored. The results showed that BDE47 exposure increased depression-like behaviors in mice but inhibited the learning memory ability of mice. The RNA sequencing analysis showed that BDE47 exposure disrupted dopamine transmission in the brain of mice. Meanwhile, BDE47 exposure reduced protein levels of tyrosine hydroxylase (TH) and dopamine transporter (DAT), activated astrocytes and microglia cells, and increased protein levels of NLRP3, IL-6, IL-1β, and TNF-α in the brain of mice. The 16 s rDNA sequencing analysis showed that BDE47 exposure disrupted microbiota communities in the intestinal contents of mice, and faecalibaculum was the most increased genus. Moreover, BDE47 exposure increased the levels of IL-6, IL-1β, and TNF-α in the colon and serum of mice but decreased the levels of tight junction protein ZO-1 and Occludin in the colon and brain of mice. In addition, the metabolomic analysis revealed that BDE47 exposure induced metabolic disorders of arachidonic acid and neurotransmitter 2-Arachidonoyl glycerol (2-AG) was one of the most decreased metabolites. Correlation analysis further revealed gut microbial dysbiosis, particularly faecalibaculum , is associated with altered gut metabolites and serum cytokines in response to BDE47 exposure. Our results suggest that BDE47 might induce depression-like behavior in mice through gut microbial dysbiosis. The mechanism might be associated with the inhibited 2-AG signaling and increased inflammatory signaling in the gut-brain axis.
研究领域:
神经科学
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药理学
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分子生物学
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微生物学
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免疫学
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DOI
PubMed
高影响因子
PLoS One
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2017
·Vol. 12
·期 3
·e0174589
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IF 2.806
Guofu Huang,Mianmian Wang,Yongyou Hu,Sihao Lv,Changfang Li
In this study, two kinds of cellulose derivatives (polyanionic cellulose (PAC) and hydroxypropylmethyl cellulose (HPMC)) were selected as stabilizers of Pd/Fe nanoparticles (NPs) to investigate their influences on the debromination performances of 2,2',4,4'-tretrabromodiphenyl ether (BDE47). Field emission scanning electron microscope (FE-SEM) images revealed that the cellulose-stabilized Pd/Fe NPs were smaller and more uniform than the bare-Pd/Fe NPs. X-ray diffractometer (XRD) and X-ray photoelectron spectroscopy (XPS) results suggested that cellulose coatings found on Pd/Fe NPs surfaces featured some antioxidation abilities, which followed the order of HPMC < PAC. Sedimentation tests demonstrated that the stabilizing power of PAC for Pd/Fe NPs was higher than that of HPMC. Fourier transfer infrared spectrometer (FTIR) results indicated that PAC molecules were bound to the Pd/Fe NPs surfaces by polar covalent bonds and hydrogen bonds, while HPMC molecules interacted with the nanoparticles by hydrogen bonds. Batch debromination test for BDE47 demonstrated that the catalytic debromination rate with cellulose-stabilized Pd/Fe NPs was higher than that with bare-Pd/Fe NPs during reaction period of 15 min. Overall, this study indicated that both celluloses are beneficial to forming smaller, more regular, stable and antioxidative Pd/Fe NPs, leading to higher debromination reactivity for BDE47 compared with the bare-Pd/Fe NPs. Therefore Pd/Fe NPs can be utilized as a promising remediation technology for the contaminated groundwater and soils.
研究领域:
环境科学
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纳米技术
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化学工程
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2019
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IF 8.355
Tao Pan,Yu Wang,Xin Yang,Xiong-fei Huang,Rong-liang Qiu
Fe(II)-catalyzed peroxymonosulfate (PMS) activation process was able to degrade persistent organic pollutants, such as polybrominated diphenyl ethers (PBDEs), but the slow transformation rate from the generated Fe(III) to Fe(II) restricted the efficiency of this process. In this study, we found that the addition of small quantities of gallic acid (GA), a model compound of natural polyphenols, in the Fe(III)/PMS process (namely GA/Fe(III)/PMS process) could exert a long-term influence on Fe(II) recycle and accelerated the degradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE47) over 72 h. Under conditions of 20.3 µM GA, 13.6 µM Fe(III) and 400 µM PMS, the degradation efficiency of BDE47 reached 85%, which was 9.4 times higher than that in the Fe(III)/PMS process. The degradation kinetics can be divided into an initial “fast stage” (k obs1 = 0.298 h −1 ) and a second “slow stage” (k obs2 = 0.021 h −1 ). Aromatic radicals such as hydroxycyclohexadienyl radical (poly-HCD ) produced by the attack of SO 4 − /HO on GA was proposed to be responsible for Fe(II) recycle in the first stage, while ring-opened products following SO 4 − /HO attack of GA-quinone mainly initiated Fe(III) reduction in the second stage. Owing to the multiple Fe(III) reduction pathways, PMS would be continuously activated by Fe(II) to form SO 4 − and HO , which were the dominant reactive species for BDE47 degradation. Finally, natural polyphenols extracted from green tea were proven effective in enhancing BDE47 degradation in Fe(III)/PMS process. This study not only provides a new way to propagate Fe(II)-activated persulfate chain reactions for the degradation of refractory organic contaminants, like PBDEs, but also sheds new insight into the reactivity of organic byproducts toward Fe in PMS-based oxidation system.
研究领域:
环境科学
·
化学
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DOI
高影响因子
APPLIED SURFACE SCIENCE
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2022
·
IF 7.392
Huajiang Huang,Zhen Wan,Didi Li,Jingjing Xiang
Recently, the hydrogenation reactions of heterogeneous frustrated Lewis pairs (FLP) catalysis based on metal oxides and corresponding reaction mechanisms were extensively studied. Generally, the hydrogenation performance of metal oxides in FLP catalysis was considered to be mainly related to the physicochemical properties of oxygen defects while the effect of solvents was ignored. Thus, the development of strategies for increasing the performances of catalysts is restricted to the regulation of oxygen defects. Herein, we reported different mechanisms for the FLP-catalyzed photocatalytic dehalogenation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE47) by In 2 O 3 in polar/non-polar solvents. Namely, in polar solvents , the Lewis basic sites of FLP transferred to the solvent molecules bonded with oxygen defects while it remains at the In 2 O 3 surface in non-polar solvents. Thus, in different non-polar solvents, the photocatalytic activities for the dehalogenation of BDE47 remained almost unchanged. On the contrary, in reactions with polar solvents, because of the inactivation of original Lewis basic sites, the photocatalytic performances were largely determined by charge densities of the Lewis basic sites in solvent molecules. The above fundamental insight into the different mechanisms of FLP-catalyzed dehalogenation in polar/non-polar solvents could provide a new strategy for the improvement of heterogeneous FLP catalysts for hydrogenation reactions.
研究领域:
环境科学
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化学
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DOI
高影响因子
Journal of Environmental Sciences
·
2025
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IF 6.3
Yinan Zhang,Bingyi Wang,Wenhui Sun,Lilai Shen,Zhiquan Liu,Binhao Wang,Jiafeng Ding,Yu Han,Hangjun Zhang
Emerging pollutants such as polystyrene-nanoplastics (PS-NPs) and polybrominated diphenyl ethers (PBDEs) are a threat to human health and ecological safety. However, NP-mediated PBDE exposure and toxicity remain poorly understood. Herein, male frogs ( Pelophylax nigromaculatus ) were exposed to 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) and 6-hydroxy-2,2′,4,4′-tetrabromodiphenyl ether (6-OH-BDE-47) alone or in combination with PS-NPs for 30 days to explore the carrier toxicity effect and mechanism of co-exposure. Transcriptome data verified that both BDE-47 and 6-OH-BDE-47 could mediate the steroid hormone synthesis signaling pathway to induce reproductive toxicity. The presence of PS-NPs increased the bioconcentration of BDE-47 and 6-OH-BDE-47 by 54.36 % and 51.49 %, respectively, and aggravate the damage of the testes. To investigate the mechanism underlying the disrupted steroidogenesis, changes in hormones of the hypothalamic-pituitary-gonadal (HPG) axis and gene expression involved in synthesis and transformation of sex hormones were assessed. AR, StAR, CYP11A1 and HSD3B1 were all downregulated, while mRNA expression levels of CYP19A1 and ESR1 were upregulated, accompanied by a decrease in testosterone and an increase in oestradiol. The results demonstrated that PS-NPs can exacerbate the estrogen-like effects of BDE-47 and 6-OH-BDE-47 in male frogs, highlighting the potential risks posed by these pollutants in aquatic ecosystems.
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高影响因子
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