Flumequine (FLU) is widely used as an antibiotic in the treatment and control of animal diseases, which may result in the presence of residues harmful to humans in food products of animal origin. A novel and fast biomimetic enzyme-linked immunosorbent assay (BELISA) was developed for the determination of FLU based on a molecularly imprinted polymer (MIP) as a selective affinity agent. With an optimized molar ratio of FLU to methacrylic acid (MAA) of 1:2 determined by molecular dynamic simulations, the MIP was directly synthesized on the surface of a 96-well plate (as solid support) with FLU as the template molecule, MAA as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, and azodiisobutyronitrile (AIBN) as the initiator. Under the optimized conditions, the BELISA method was established with a sensitivity (IC 50 ) of 0.141 μg/mL and a detection limit (IC 15 ) of 1.09 × 10 −3 μg/mL. The developed method was applied to determine FLU concentrations in spiked beef and shrimp samples, with satisfactory recovery values ranging from 80.7 to 92.2% with relative standard deviations ( n = 3) between 3.3% and 7.0%. This method can be used for the rapid determination of FLU in animal foods and has promising applications in food safety monitoring.
In this paper, a sensitive biomimetic enzyme-linked immunoassay (BELISA) method based on an Au@Pt@Au composite nanozyme label was developed for histamine (HA) detection. A molecular imprinted polymer prepared directly on the 96-well plate served as a biomimetic antibody. A BELISA method was developed for HA standard solutions at concentrations ranging from 0.01 to 100.00 mg/L. Under optimal conditions, the novel BELISA method had a low limit of detection (0.069 mg/L) and high sensitivity (7.20 mg/L). The HA spiked in yellow rice wine and liqueur samples were detected by this BELISA method with the recoveries ranging from 84.28% to 108.82%. There was no significant difference of the results between the BELISA method and HPLC for the detection of HA in the red and white grape wine samples.
Zhaorui Song,Xinhui Zhai,Chenrui Jiang,Rong Chen,Sijing Ye,Jinzhe Tong,Pierre Dramou,Hua He
A novel bionic enzyme-linked immunosorbent assay (BELISA) based on double-antibody sandwich method is firstly designed for the detection of carbamazepine (CBZ) in human serum samples. In this BELISA system, cucurbit[7]uril (CB[7]) is employed as an artificial capture antibody (cAb), and molecularly imprinted polymers (MIPs) is used as an artificial detection antibody (dAb). Nanozymes (PdNPs) as signal generators are integrated with MIPs. This couple of bionic antibodies exhibits not only the excellent physical and chemical stability, but also the superior molecular recognition ability. Based on two bionic antibodies that can selectively recognize different sites of CBZ molecule, a new BELISA method has been constructed for the first time. The proposed BELISA method displays a good linear relationship ranging from 2 to 20 μg mL -1 . The detection limit is 0.37 μg mL -1 , which can well meet clinical testing demand. It provides a more stable and economical method for clinical therapeutic drug monitoring (TDM).
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