DYES AND PIGMENTS
·
2020
·
IF 4.613
Kang-Nan Wang,Xiang-Jun Peng,Yi Li,Hai-Tao Tang,Ying-Ming Pan,Liang He
In addition to being associated with energy supply, lipid droplets (LDs) play vital roles in many other important physiological and some pathological processes. Fluorescent probes are useful tools for studying the biological functions of LDs. In this study, we develop three new highly fluorescent LD-specific bioprobes, which can be excited by one- and two-photon, with favorable biocompatibility. They have little crosstalk with some commonly used commercially-available green- and red-emissive probes, such as BODIPY 493/503, MitoTracker Red CMXRos (MTR) and LysoTracker Red DND-99 (LTR), thus allowing the simultaneous multicolor imaging. Notably, one of the probes, BIP-1 , has superior photostability and allows excellent 3D imaging of cellular LDs. BIP-1 can be used to monitor the number and morphological changes of LDs, and the interactions of LDs with other organelles (e.g. mitochondria) under specific physiological conditions (e.g. starvation). Overall, BIP-1 is a promising alternative fluorescent bioprobe for live-cell LD-specific imaging and long-term tracking during LD-related research.
研究领域:
细胞生物学
·
生物成像
·
分子生物学
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DOI
高影响因子
Chinese Journal of Natural Medicines
·
2026
·Vol. 24
·期 4
·427-439
·
IF 7.5
Weidan Tan,Xinyu Wei,Changsheng Li,Huilan Wei,Fei Yu,Xiaoju Shen,Xiaoxiang Mo,Meifen Wei,Shangping Xing,Zhuo Luo,Jie Yang
Lung squamous cell carcinoma (LUSC) is a prevalent and aggressive form of lung cancer with limited therapeutic options. Sanguinarine (SAG), a prominent benzophenanthridine alkaloid derived from Zanthoxylum nitidum (Roxb.) DC, exhibits established anti-tumor activity; however, its molecular mechanisms in LUSC remain incompletely defined. In this study, the anti-cancer effects and underlying mechanisms of SAG were systematically investigated in vitro and in vivo . Cell viability and death were evaluated using methyl thiazolyl tetrazolium (MTT) assays, colony formation assays, flow cytometry, transmission electron microscopy (TEM), and Western blotting (WB). Drug affinity responsive target stability (DARTS) combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS), molecular docking, cellular thermal shift assay (CETSA), and surface plasmon resonance (SPR) were employed to identify and validate molecular targets of SAG. The results demonstrated that SAG simultaneously induces apoptosis and ferroptosis in LUSC cells by directly targeting the endoplasmic reticulum (ER) chaperone binding immunoglobulin protein (BiP). Silencing of BiP markedly attenuated SAG-induced apoptosis and ferroptosis, confirming its essential role in this process. Mechanistically, SAG up-regulates BiP expression and activates the protein kinase R-like endoplasmic reticulum kinase (PERK)/eIF2α/C/EBP homologous protein (CHOP)/GADD34 signaling axis of ER stress (ERS), ultimately leading to dual induction of apoptosis and ferroptosis in vitro and in vivo .
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DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2026
·
IF 2.5
Shoukang Du,Yuhan Wang,Ting Gang Chew
Cells maintain proteostasis by sequestering misfolded proteins into deposition sites. Aggregation-prone endoplasmic reticulum (ER) proteins form membrane-bound nuclear compartments that are cleared during cell division, yet the mechanisms underlying their spatial organization remain unclear. Here, using transcriptomic and proteomic analyses, we identified the ER-localized Hsp70 chaperone BiP as a key player. Genetic depletion or chemical inhibition of BiP prevented nuclear aggregate formation, while manipulating BiP regulators perturbed the aggregate formation. BiP-driven aggregation precedes the inner nuclear membrane synthesis that encapsulated the aggregates. Under proteostatic stress, nuclear aggregates localized adjacent to ER-derived aggregates. Our findings demonstrate that BiP is essential for organizing ER-derived aggregates in the nucleus, which further regulate nuclear proteostasis through spatial interactions with nuclear aggregates.
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DOI
PubMed
高影响因子
BIOLOGICAL RESEARCH
·
2024
·Vol. 57
·期 1
·1-12
·
IF 4.3
Ma Lin-Wei,Liu Yu-Fan,Zhang Hui,Huang Chang-Jun,Li Ang,Qu Xin-Zhe,Lin Jia-Piao,Yang Yan,Yao Yong-Xing
Studies have suggested that endoplasmic reticulum stress (ERS) is involved in neurological dysfunction and that electroacupuncture (EA) attenuates neuropathic pain (NP) via undefined pathways. However, the role of ERS in the anterior cingulate cortex (ACC) in NP and the effect of EA on ERS in the ACC have not yet been investigated. In this study, an NP model was established by chronic constriction injury (CCI) of the left sciatic nerve in rats, and mechanical and cold tests were used to evaluate behavioral hyperalgesia. The protein expression and distribution were evaluated using western blotting and immunofluorescence. The results showed that glucose-regulated protein 78 (BIP) and inositol-requiring enzyme 1α (IRE-1α) were co-localized in neurons in the ACC. After CCI, BIP, IRE-1α, and phosphorylation of IRE-1α were upregulated in the ACC. Intra-ACC administration of 4-PBA and Kira-6 attenuated pain hypersensitivity and downregulated phosphorylation of IRE-1α, while intraperitoneal injection of 4-PBA attenuated hyperalgesia and inhibited the activation of P38 and JNK in ACC. In contrast, ERS activation by intraperitoneal injection of tunicamycin induced behavioral hyperalgesia in naive rats. Furthermore, EA attenuated pain hypersensitivity and inhibited the CCI-induced overexpression of BIP and pIRE-1α. Taken together, these results demonstrate that EA attenuates NP by suppressing BIP- and IRE-1α-mediated ERS in the ACC. Our study presents novel evidence that ERS in the ACC is implicated in the development of NP and provides insights into the molecular mechanisms involved in the analgesic effect of EA.
研究领域:
神经科学
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疼痛管理
·
分子生物学
·
针灸
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2026
·Vol. 117
·期 5
·e70121
·
IF 2.9
Bangqi Fu,Guoqiang Chen,Xuedong Chen,Linyuan Chen,Yanjun Jiang,Lei Wang,Chengbo Gu,Haibo Zhang
Due to its dense crystalline structure and extensive hydrogen bonding network, cellulose exhibits limited reactive accessibility and reactivity, which severely restricts the reaction efficiency, degree of substitution, and uniformity of cellulose modifications such as carboxymethylation. To overcome these limitations, this study developed a novel glycerol-based pretreatment strategy to disrupt the hydrogen bonding network of bacterial cellulose (BC), thereby improving its reactive accessibility. The results confirmed that glycerol pretreatment significantly enhanced the reactivity of BC. The reaction time for preparing carboxymethyl cellulose (CMC) from glycerol-pretreated powdered BC was reduced from 2 to 1 h, representing a 100% improvement in reaction efficiency. In addition, glycerol pretreatment facilitated the exposure of hydroxyl groups in BC, thereby increasing the degree of substitution of CMC from 0.297 to 0.433 and from 0.427 to a peak value of 0.626, an approximately 46% enhancement. In addition, the CMC films prepared from glycerol-pretreated BC exhibited significantly improved light transmittance, reaching over 95% in the wavelength range of 300–1000 nm. This study provides theoretical support and technical guidance for the efficient production of CMC, expands its application potential in high-end fields, and promotes the high-value utilization of cellulose resources. Graphical Enhanced carboxymethylation of bacterial cellulose via glycerol pretreatment.
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2025
·Vol. 116
·期 4
·e70032
·
IF 3.2
Ying Guo,Bei Tian,Yi Xie,Xiuxia Sun,Jianxi Xiao
Skin aging is a multifaceted process marked by the destruction of skin structure and the diminishment of skin function, significantly impacting both physical and mental health. Injectable hydrogels are promising for skin repair, but chemical crosslinking in most hydrogels can cause cytotoxicity, whereas agarose hydrogels avoid crosslinking yet face injectability challenges. We have herein developed green agarose hydrogel implants with high biocompatibility for skin repair and regeneration in photoaged skin. The hydrogel is prepared by utilizing agarose's ability to dissolve at high temperatures and gel at low temperatures. This process yields implants with particle sizes predominantly ranging from 100 to 150 μm. The agarose hydrogel implant exhibits excellent injectability, with a steady injection force curve consistently around 4 N. The agarose hydrogel implant promotes the proliferation of human dermal fibroblasts and does not induce hemolysis or pyrogenic reactions. In a photoaging mouse model, the agarose hydrogel implant improves skin density and hydration, reduces transepidermal water loss, and stimulates collagen regeneration. This agarose hydrogel implant provides a novel approach to repairing aged skin and holds significant potential in the fields of skin health and tissue regeneration. Graphical
研究领域:
生物材料学
·
皮肤科学
·
再生医学
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2019
·Vol. 110
·期 9
·e23318
·
IF 1.845
Yan Xiao,Chenna Tang,Yang Chen,Meidong Lang
Stimuli-responsive polymers that can undergo conformational changes with external triggers have enabled themselves as smart materials for various utilizations, among which biodegradability is of particular importance to be engineered for biomedical application. In this study, a thermo and pH dual responsive polypeptide ( N , N -dimethylaminoethyl acrylate-modified poly( l -cysteine)) (PLC- g -DMAEA) was prepared by the combination of N -carboxyanhydride ring-open polymerization and thiol-ene click chemistry. The biodegradable poly( l -cysteine) (PLC) with pendant thiol groups provided an easily clickable backbone for postmodification, which was demonstrated by reacting with a well-known monomer of N , N -dimethylaminoethyl acrylate (DMAEA) to achieve both temperature and pH responsiveness. The irreversible thermo-response of PLC- g -DMAEA could be attributed to the ordered β-sheets formed upon heating, leading to the trapped side groups with poor water accessibility. Moreover, this copolymer precipitated at pH ranging from 7.5 to 9.7, but protonation of tertiary amine groups (pH < 7.5) and salt forming of masked thiol groups (pH > 9.7) rendered it soluble in water. Our results revealed that a ready available vinyl monomer could be easily clicked onto the biodegradable PLC and its stimuli responsiveness would be reserved. Moreover, the primary and secondary structures of PLC might influence the conformation, thus leading to the unique responsive behavior of the resulted copolymer. Graphical
研究领域:
生物材料
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高分子化学
·
生物医药
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2018
·Vol. 109
·期 6
·e23222
·
IF 1.99
Yuanzi Wu,Zhen Cai,Shuigen Wu,Wenli Xiong,Shanyun Ma
A recoverable and thermoresponsive polymer-protein bioconjugate is synthesized and employed in the purification of protein with free sulfhydryl groups. Initiator with disulphide was modified on the cysteine residue of the target protein. Poly(N-isopropylacrylamide) exhibiting a lower critical solution temperature was grown from the protein. The resulting protein–polymer conjugate was successfully thermoprecipitated and separated from other proteins. The approach was demonstrated with bovine serum albumin with the recycling yield of 76.4%. Enzyme activity test with papain verified the reversible polymer modification protected protein under extreme environments without affecting the functionality of the protein. This study implies the favorable potential of chemo-selective enriching and purification of proteins. Graphical
研究领域:
生物技术
·
化学工程
·
材料科学
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2018
·Vol. 109
·期 7
·e23223
·
IF 1.99
Yue Yang,Zuyuan Luo,Ying Zhao
The scaffolds for stem cell-based bone tissue engineering should hold the ability to guide stem cells osteo-differentiating. Otherwise, stem cells will differentiate into unwanted cell types or will form tumors in vivo. Alginate, a natural polysaccharide with great biocompatibility, was widely used in biomedical applications. However, the limited bioactivity and poor osteogenesis capability of pristine alginate hampered its further application in tissue engineering. In this work, a bone forming peptide-1 (BFP-1), derived from bone morphogenetic protein-7, was grafted to alginate polymer chains to prepare peptide-decorated alginate porous scaffolds (pep-APS) for promoting osteo-differentiation of human mesenchymal stem cells (hMSCs). SEM images of pep-APS exhibited porous structure with about 90% porosity (pore size 100–300 μm), which was appropriate for hMSCs ingrowth. The adhesion, proliferation and aggregation of hMSCs grown on pep-APS were enhanced in vitro. Moreover, pep-APS promoted the alkaline phosphatase (ALP) activity of hMSCs, and the osteo-related genes expression was obviously up-regulated. The immunochemical staining and western blot analysis results showed high expression level of OCN and Col1a1 in the hMSCs grown on pep-APS. This work provided a facile and valid strategy to endow the alginate polymers themselves with specific bioactivity and prepare osteopromoting scaffold with enhanced osteogenesis ability, possessing potential applications in stem cell therapy and regenerative medicine. Graphical
研究领域:
组织工程
·
干细胞生物学
·
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2017
·Vol. 107
·期 11
·e23041
·
IF 1.908
Xiaokai Su,Xinyu Zhou,Zhengzhong Tan,Chuncai Zhou
A series of amphiphilic diblock copolypeptides (K 30 - b -F 15 , K 30 - b -F 30 , and K 30 - b -F 45 ) were synthesized via N -carboxy-α-amino-anhydride ring-opening polymerization. The copolypeptides had excellent antibacterial efficacy to both Gram positive ( S. aureus ) and Gram negative ( E. coli ) bacteria. The minimum inhibitory concentrations (MICs) against E. coli and S. aureus are 8 μg mL −1 and 2 μg mL −1 , respectively, lower than most natural and artificial antimicrobial peptides (AMPs). The morphological changes of the bacteria treated with diblock copolypeptides were investigated by transmission electron microscopy; the results proved that the diblock copolypeptides had a similar antibacterial pore-forming mechanism to natural cationic peptides. This was confirmed by laser scanning confocal microscope images. CCK-8 results and the MICs showed that the diblock copolypeptides have high selectivity to bacteria, which suggested that the diblock copolypeptides could be excellent candidates to replace traditional antibiotics in future. Graphical
研究领域:
生物材料学
·
微生物学
·
聚合物化学
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2020
·Vol. 111
·期 6
·e23354
·
IF 1.854
Xiaolin Xiao,Yuyuan Zhu,Jiexin Liao,Tao Wang,Weixiang Sun,Zhen Tong
Hydrogel dressings have significant advantages such as absorption of tissue exudate, maintenance of proper moist environment, and promotion of cell proliferation. However, facile preparation method and high-efficient antibacterial hydrogel dressings are still a great challenge. In this study, a facile approach to prepare antibacterial nanocomposite hydrogel dressing to accelerate healing was explored. The hydrogels consisted of quaternized chitosan and chemically cross-linked polyacrylamide, as well as silver nanoparticles (AgNPs) stabilized by chitosan. The synthesis of the hydrogels including the formation of AgNPs and polymerization of acrylamide was accomplished simultaneously under UV irradiation in 1 hour without adding initiator. The hydrogels showed favorable tensile strength of ∼100 kPa with elongation at break over 1000% and shear modulus of ∼10 4 Pa as well as suitable swelling ratio, which were appropriate for wound dressing. The combination of quaternized chitosan and AgNPs exhibited high-efficient and synergetic antibacterial performance with low cytotoxicity. In vivo animal experiments showed that the hydrogel can effectively prevent wound infection and promote wound healing. This study provides a facile method to produce antibacterial hydrogel wound dressing materials. Graphical
研究领域:
生物材料学
·
医学工程
·
纳米医学
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DOI
PubMed
高影响因子
BIOPOLYMERS
·
2018
·Vol. 110
·期 4
·e23243
·
IF 1.99
Xiaohui Fu,Jiliang Tian,Zheng Li,Jing Sun,Zhibo Li
A series of pegylated polypeptoids have been readily synthesized by a strategy combining ring-opening polymerization (ROP) and thiol-yne photoaddition. The polypeptoids simultaneously incorporated branched oligo(ethylene glycol) (OEG) units and thioether bonds in the side-chains. All the polypeptoids are readily soluble in aqueous solution and show reversible thermo-responsive properties. The cloud points (CPs) were demonstrated to be readily tunable in the range of ~25 °C-60 °C by varying the chemical composition, OEG chain length and the degree of polymerization. Attractively, the chemical compositions of the side chains are readily tunable via adjusting the molar ratios of a mixture of thiol terminated OEG molecules, which avoid synthesizing new monomers or copolymerization of different monomers. Further, the oxidation/reduction of thioether groups shows significant influence on the CPs, providing a second stimulus to tune the phase transition behaviors. Considering the biocompatibility and degradability, the dual-responsive polypeptoids are potential candidates for various biomedical or biotechnological applications. Graphical
研究领域:
生物材料学
·
高分子化学
·
生物医药工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）