Toxics
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2025
·Vol. 13
·期 11
·946
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IF 4.1
Jin Liu,Yankun Zhang,Fengyuan Chen,Dandan Duan,Xiaoping Diao
In the environment, the coexistence of microplastics (MPs) with other pollutants may either enhance or reduce the toxicity of MPs themselves or the co-occurring pollutants toward microalgae. This phenomenon is particularly notable when MPs interact with emerging pollutants, such as ultraviolet absorbers. This study investigates the single and combined exposure effects of ultraviolet absorber (Butyl methoxydibenzoylmethane, BMDM, 50 μg/L) and MPs (Polystyrene, PS, 10 mg/L, d = 1 μm) onChlorellasp. with a stress duration of 7 days. The results showed that cell density, chlorophyll a (Chla) concentration, and physical properties of cell surface integrity were higher in the combined stress group compared to the BMDM single stress group. Furthermore, transcriptome sequencing analysis revealed that the number of differentially expressed genes (DEGs) in the combined exposure group (885 DEGs) was lower than in the single exposure groups (BMDM: 1870 DEGs and PS: 9109 DEGs). Transcriptomic profiling indicated that individual stressors of BMDM and PS disrupted 113 and 123 pathways, respectively, predominantly associated with protein synthesis and energy metabolism. Conversely, combined exposure significantly enriched 86 pathways, including ribosome function and oxidative phosphorylation, thereby manifesting an antagonistic effect. This study provides new insights into the effects of BMDM and PS onChlorellasp. and offers valuable information for the risk assessment of multiple pollutants.
研究领域:
环境毒理学
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藻类学
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生态毒理学
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转录组学
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海洋污染
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Tian Liu,Yun Zhang,Ze Wu,Chen-jing Zhao,Xi Dong,He-xin Gong,Bing Jin,Miao-miao Han,Jin-jia Wu,Yi-kai Fan,Nan Li,Ying-xia Xiong,Zi-qian Zhang,Zheng-qi Dong
The macrophage to myofibroblasts transition (MMT) has been reported as a newly key target in renal fibrosis. Lycium barbarum L. is a traditional Chinese medicine for improving renal function, in which its polysaccharides (LBPs) are the mainly active components. However, whether the role of LBPs in treating renal fibrosis is related to MMT process remain unclear. The purpose of this study was to explore the relationship between the regulating effect on MMT process and the anti-fibrotic effect of LBPs. Initially, small molecular weight LBPs fractions (LBP-S) were firstly isolated via Sephadex G-100 column. Then, the potent inhibitory effect of LBP-S on MMT process was revealed on bone marrow-derived macrophages (BMDM) model induced by TGF-β. Subsequently, the chemical structure of LBP-S was elucidated through monosaccharide, methylation and NMR spectrum analysis. In vivo biodistribution characteristics studies demonstrated that LBP-S exhibited effectively accumulation in kidney via intraperitoneal administration. Finally, LBP-S showed a satisfactory anti-renal fibrotic effect on unilateral ureteral obstruction operation (UUO) mice, which was significantly reduced following macrophage depletion. Overall, our findings indicated that LPB-S could alleviate renal fibrosis through regulating MMT process and providing new candidate agents for chronic kidney disease (CKD) related fibrosis treatment.
研究领域:
药理学
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肾病学
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分子生物学
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中医药学
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Xiaoliang Dong,Lu Qu,Bingxin Wang,Xiaowei Sha,Bo Wu,Yudong Sun,Xiaohua Pan,Juan Xiong,Jia Sun,Li-Long Pan
ABSTRACT Background Cardiovascular disease is one of the main causes of global mortality, and there is an urgent need for effective treatment strategies. Gut microbiota-dependent metabolite trimethylamine-N-oxide (TMAO) promotes the development of cardiovascular diseases, and shizukaol C, a natural sesquiterpene isolated from Chloranthus multistachys with various biological activities, might exhibit beneficial role in preventing TMAO-induced vascular inflammation. Purpose The purpose of this study was to investigate the anti-inflammatory effects and the underlying mechanisms of shizukaol C on TMAO-induced vascular inflammation. Methods The effect and underlying mechanism of shizukaol C on TMAO-induced adhesion molecules expression, bone marrow-derived macrophages (BMDM) adhesion to VSMC were evaluated by western blot, cell adhesion assay, co-immunoprecipitation, immunofluorescence assay, and quantitative Real-Time PCR, respectively. To verify the role of shizukaol C in vivo , TMAO-induced vascular inflammation model were established using guidewire-induced injury on mice carotid artery. Changes in the intima area and the expression of GSTpi, VCAM-1, CD68 were examined using haematoxylin-eosin staining, and immunofluorescence assay. Results Our data demonstrated that shizukaol C significantly suppressed TMAO-induced adhesion molecule expression and the bone marrow-derived macrophages (BMDM) adhesion in vascular smooth muscle cells (VSMC). Mechanically, shizukaol C inhibited TMAO-induced c-Jun N-terminal kinase (JNK)-nuclear factor-kappa B (NF-κB)/p65 activation, and the JNK inhibition was dependent on the shizukaol C-mediated glutathione-S-transferase pi (GSTpi) expression. By further molecular docking and protein-binding analysis, we demonstrated that shizukaol C directly binds to Keap1 to induce Nrf2 nuclear translocation and upregulated GSTpi expression. Consistently, our in vivo experiment showed that shizukaol C elevated the expression level of GSTpi in carotid arteries and alleviates TMAO-induced vascular inflammation. Conclusion : Shizukaol C exerts anti-inflammatory effects in TMAO-treated VSMC by targeting Keap1 and activating Nrf2-GSTpi signaling and resultantly inhibits the downstream JNK-NF-κB p65 activation and VSMC adhesion, and alleviates TMAO-induced vascular inflammation in vivo , suggesting that shizukaol C may be a potential drug for treating TMAO-induced vascular diseases.
研究领域:
药理学
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心血管疾病
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炎症
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）