Hydrogen sulfide (H 2 S) is a gaseous signaling molecule that extends the postharvest storage period of fruit. Biaxially oriented polyethylene (BOPE) films are widely used in fruit packaging due to their excellent properties. By analyzing the phenotypic characteristics of strawberry fruits by comparing with the control, PE and BOPE or in combination with H 2 S at 4 ℃, it was shown that the H 2 S+BOPE treatment reduced decay and water loss, and maintained the best lustre and firmness than other groups. A total of 7,258 co-differentially expressed genes (DEGs) were identified by comparing H 2 S, H 2 S+PE and H 2 S+BOPE with Control by transcriptome analysis. GO, WGCNA and KEGG analyses showed that DEGs were mainly enriched in starch and sucrose metabolism, pigment metabolism and antioxidant activity from the MEyellow and MEblack modules. Moreover, these candidate genes were screened, and the expression level of CHS , PAL and DFR were significantly decreased in H 2 S+BOPE group than other groups by RT-qPCR. Physiological assessments indicated that H 2 S+BOPE treatment significantly delayed chlorophyll degradation, anthocyanin accumulation and TA content, increased reducing sugar content, carotenoid and POD activity, and maintained low MDA content in strawberries. PCA and heatmap showed that POD and pigments were main factors and the correlation analysis proved that POD activity exhibited a positive correlation with the expression of DFR (0.57) and bHLH3 (0.56), carotenoid content (0.54), and it showed a negative correlation with chlorophyll contents (-0.37). These results indicated that BOPE could effectively improve the appearance and antioxidant activity of strawberry fruits, thereby prolonging their postharvest storage period.
Biaxially oriented polyethylene (BOPE) is a high-strength, lightweight polyethylene material with superior barrier properties. In this study, peaches were packaged using either polyethylene (PE) or BOPE film. The peaches packaged in BOPE film maintained better firmness, color retention, antioxidant content, and nutritional quality than control peaches and those packaged in PE film. Transcriptome sequencing revealed 1041 differentially expressed genes in BOPE-packaged fruit compared with that in the control, and these were enriched in the pyruvate metabolism, flavonoid biosynthesis, and glutathione metabolism pathways. Physiological analyses demonstrated that BOPE packaging reduced malondialdehyde accumulation and increased peroxidase and phenylalanine ammonia-lyase activity, potentially limiting membrane peroxidation and senescence. BOPE-packaged fruit also retained higher levels of soluble sugars, reducing sugars, free amino acids, and proline, which are critical for fruit flavor and stress adaptation. Together, these results underscore the potential of BOPE as a novel packaging material for the preservation of fruits and vegetables.
危险品化学品经营许可证（带存储）