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NEW JOURNAL OF CHEMISTRY · 2013 ·Vol. 37 ·期 7 ·2174-2178 · IF 2.966

Colorimetric detection of glutathione in human blood serum based on the reduction of oxidized TMB

Xing Liu,Qi Wang,Yu Zhang,Lichun Zhang,Yingying Su,Yi Lv

In this paper, a novel and simple colorimetric method for the determination of glutathione (GSH) was developed. The BSA–MnO2 NPs possess oxidase-like activity that can catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB), and the existence of GSH can cause reduction of oxidized TMB along with a visual color variation. A good linear relationship can be obtained from 0.26 to 26 μM with a limit of detection of 0.1 μM. Furthermore, the good recoveries for serum samples indicated that the present colorimetric methodology was feasible, simple and sensitive, with a promising application in routine analysis of biosamples.

CAS / MDL: 69-93-2

研究领域: 分析化学 · 生物化学 · 纳米技术

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ANALYTICA CHIMICA ACTA · 2018 · IF 5.123

A multicolorimetric assay for rapid detection of Listeria monocytogenes based on the etching of gold nanorods

Yushen Liu,Juan Wang,Chao Zhao,Xiaoxiao Guo,Xiuling Song,Wei Zhao,Sijie Liu,Kun Xu,Juan Li

Listeria monocytogenes ( L. monocytogenes ) is one of the most common food-borne pathogens . The authors describe a sensitive and reliable multicolorimetric assay for L. monocytogenes using a sensing system based on TMB 2+ etching of gold nanorods . Apt-MNP was used as the capture probe, and IgY-BSA-MnO 2 NPs was chosen as an oxidase-like nano-artificial enzyme to oxidize TMB to generate TMB 2+ . Under the optimized conditions, the longitudinal shift of localized surface plasmon resonances had a linear correlation with the L. m onoc ytogenes concentration in the range between 10 to 10 6  cfu mL −1 . Meanwhile, the sensing system can generate vivid color responses as colorful as a rainbow, and the limit of detection is as low as 10 cfu mL −1 at a glance. Recoveries ranging from 97.4 to 101.3% are found when analyzing spiked food samples without pre-enrichment. In our perception, it shows promise in rapid instrumental and on-site visual detection of L. monocytogenes .

研究领域: 微生物学 · 纳米技术 · 食品安全

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Colorimetric detection of glutathione in human blood serum based on the reduction of oxidized TMB

A multicolorimetric assay for rapid detection of Listeria monocytogenes based on the etching of gold nanorods