CLAYS AND CLAY MINERALS
·
2019
·Vol. 67
·期 4
·275-282
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IF 1.835
Liu Xiaonan,Zhang Youkui,Bian Liang,Li Jinshan
The adsorption of bovine serum albumin (BSA) protein is important for protein research but remains a great challenge. Here, the hydrothermal method and calcination in a hydrogen-argon mixed atmosphere were used to obtain ordered mono-crystalline magnetic Fe 3 O 4 nanorings with controllable size and uniform structure, which were applied to the adsorption of BSA protein. The results showed that the magnetic Fe 3 O 4 nanorings prepared have the advantages of good crystallinity, controllable morphology, and excellent magnetic response. The Fe 3 O 4 nanorings had an outer diameter of ~160–180 nm, inner diameter of ~80–110 nm, and height of 70 to ~110 nm. The amount of BSA adsorbed by magnetic Fe 3 O 4 nanorings increased with increasing protein concentration; when the concentration of BSA was ~2.75 mg·mL -1 the adsorption amount approached saturation. When the pH value of the solution was ~5, the Fe 3 O 4 nanorings absorption of BSA was greatest. With increasing adsorption time, the amount adsorbed increased gradually. Adsorption equilibrium was reached after 3 h. According to the formula, the saturated adsorption capacity of BSA per gram of magnetic Fe 3 O 4 nanorings was 325.2 mg.
.
研究领域:
生物化学
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材料科学
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2011
·Vol. 121
·期 1
·352-358
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IF 1.24
Yang Yu,Tingli Lu,Wen Zhao,Weiguang Sun,Tao Chen
A series of biodegradable PEG-containing polyanhydrides composed of sebacic acid, 1, 6-bis( p -carboxyphenoxy) hexane, and poly (ethylene glycol) (PEG) were used as matrix material for BSA-loaded microspheres. The effects of polymer composition on the microsphere size, entrapment efficiency, in vitro degradation, and in vitro protein release were studied. Microspheres in the size range of 0.8–10 μm were fabricated via a modified double emulsion method and were characterized using scanning electron microscopy. As the content or the molecular weight of PEG was increased in the copolymer, a proportional increase was found in the particle size and the efficiency of BSA entrapment. The in vitro degradation rate of particles could be controlled by varying the polymer composition, increasing as the PEG proportion increased. In vitro release studies of BSA from polyanhydride microspheres revealed that the increased amounts of PEG within microspheres could accelerate the release rate of protein. These studies indicate that the PEG-containing polyanhydrides hold potential for protein delivery applications. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
研究领域:
生物材料
·
药物递送
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DOI
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Ming Ying,Qin Li,Jingbo Wu,Yihang Jiang,Zhourui Xu,Mingze Ma,Gaixia Xu
Breast cancer is characterized by the uncontrolled proliferation of breast epithelial cells under the action of a variety of carcinogens. Although HER2-inhibitors were currently applied for HER2-positive breast cancer patients, they didn’t work for patients with resistance to HER2-targeted anti-cancer drugs. In this work, we prepared novel CuS@BSA-NB2 nanoparticles (NPs) for breast cancer photothermal therapy (PTT). The NPs had good biocompatibility due to the Bovine Serum Albumin (BSA) encapsulating and excellent targeting to HER2 because of nanobody 2 (NB2). Under 808 nm laser irradiation, CuS@BSA-NB2 NPs had high photothermal conversion efficiency and photothermal stability. Meanwhile, we constructed a stable cell line of MDA-MB-231/HER2 with a high expression of HER2 protein. Immunofluorescence and ICP-MS assays showed that CuS@BSA-NB2 NPs can be specifically enriched and be ingested in MDA-MB-231/HER2 cells. Furthermore, CuS@BSA-NB2 NPs had shown a more significant photothermal treatment effect than CuS@BSA under certain treatment conditions for MDA-MB-231/HER2. In addition, the cytotoxicity assay demonstrated that CuS@BSA-NB2 NPs had a low toxicity for MDA-MB-231/HER2 cells. The above results suggested that CuS@BSA-NB2 NPs were great photothermal therapeutic agents to reduce the malignant proliferation of breast epithelial cells and have potential for breast cancer therapy.Keywords: CuS@BSA-NB2 nanoparticles, photothermal therapy, HER2, NB2, MDA-MB-231/HER2
研究领域:
癌症研究
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纳米技术
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治疗学
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2014
·Vol. 43
·期 26
·10057-10063
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IF 4.097
Xin Yang,Junhai Wang,Dongyue Su,Qingdong Xia,Fang Chai,Chungang Wang,Fengyu Qu
Rapid and sensitive detection of 2,4,6-trinitrotoluene (TNT) and 4-nitrophenol (4-NP) has attracted considerable attention due to their wide applications as nitroaromatic explosive materials. A novel fluorescence method for TNT and 4-NP based on bovine serum albumin functionalized fluorescent gold nanoclusters (BSA Au-NCs) has been developed. The detection probe BSA Au-NCs can be used as a fluorescent probe for the sensitive and selective detection of TNT and 4-NP simultaneously. A good linearity of fluorescence detection using BSA Au-NCs as a fluorescent probe was observed for TNT and 4-NP concentrations in the range of 10−8–5 × 10−5 M and 10−9–5 × 10−5 M, with a detection limit of 10 nM and 1 nM, respectively. The high specificity of TNT and 4-NP with BSA Au-NCs interactions provided excellent selectivity towards detecting TNT and 4-NP over other relevant nitroaromatic compounds. This system can be applied to test strips to detect TNT and 4-NP with high sensitivity and selectivity. The vapour of TNT and 4-NP can be detected using BSA Au-NCs test paper within 1 min with a detection limit of 10 pM and 1 pM.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2022
·Vol. 20
·期 1
·1-15
·
IF 9.429
Zhang Ruiyi,Liu Teng,Li Wanzhen,Ma Zhiyuan,Pei Pei,Zhang Weiwei,Yang Kai,Tao Yugui
Tumor microenvironment (TME), characterized by high glutathione (GSH), high hydrogen peroxide (H2O2) and acidic pH levels, is favorable for the growth, invasion and metastasis of cancer cells. Taking advantage of the specific characteristics of tumors, TME-responsive GCBD NPs are designed to deliver nanoscale coordination polymers (NCPs, GA-Cu) and chemotherapy drugs (doxorubicin, DOX) based on bovine serum albumin (BSA) nanocarriers into cancer cells for combined chemodynamic therapy (CDT) and chemotherapy. In an acidic environment, GCBD NPs could release approximately 90% copper ions, which can not only consume overexpressed GSH to modulate the TME but can also react with endogenous H2O2 in a Fenton-like reaction to achieve the CDT effect. Meanwhile, the released DOX could enter the nucleus of tumor cells and affect their proliferation to achieve efficient chemotherapy. Both in vitro and in vivo experiments showed that GCBD NPs had good biosafety and could effectively inhibit the growth of cancer cells. GCBD NPs are promising as a biocompatible nanoplatform to exploit TME characteristics for combined chemo and chemodynamic therapy, providing a novel strategy to eradicate tumors with high efficiency and specificity.Graphical Abstract
研究领域:
肿瘤学
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纳米医学
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生物材料学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Ying-Ying Pei,Jin-Tao Wang,Lin Yuan,Yi Luo,Xin-Yue Niu,Xing Rong,Lin Jin,Qing-Feng Li
Protein hydrogels have attracted increasing attention because of their excellent biodegradability and biocompatibility, but frequently suffer from the single structures and functions. As a combination of luminescent materials and biomaterials, multifunctional protein luminescent hydrogels can exhibit wider applications in various fields. Herein, we report a novel, multicolor tunable, injectable, and biodegradable protein-based lanthanide luminescent hydrogel. In this work, urea was utilized to denature BSA to expose disulfide bonds , and tris(2-carboxyethyl)phosphine (TCEP) was employed to break the disulfide bonds in BSA to generate free thiols. A part of free thiols in BSA rearranged into disulfide bonds to form a crosslinked network. In addition, lanthanide complexes (Ln(4-VDPA) 3 ), containing multiple active reaction sites, could react with the remaining thiols in BSA to form the second crosslinked network. The whole process avoids the use of nonenvironmentally friendly photoinitiators and free radical initiators. The rheological properties and structure of hydrogels were investigated, and the luminescent performances of hydrogels were studied in detail. Finally, the injectability and biodegradability of hydrogels were verified. This work will provide a feasible strategy for the design and fabrication of multifunctional protein luminescent hydrogels, which may have further applications in biomedicine , optoelectronics, and information technology.
研究领域:
生物医学
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光学材料
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高分子化学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Miaomiao Ma,Yuhong Qi,Chuanjun Gu,Zhanping Zhang
Revealing the adsorption behavior of proteins on amphiphilic polymers is an interesting study. For the first time, the adsorption behavior of bovine serum albumin (BSA) protein on the surface of polyethylene glycol polystyrene (PEG-PS) amphiphilic polymer membrane is developed by molecular dynamics (MD) simulations and experiments. We found that PEG-PS has the ability to resist BSA protein adsorption. The adsorption of BSA on its surface undergoes a process of "rotation-preadsorption-desorption-final adsorption". After adsorption, the helical structure of BSA underwent "tight-loose-unwinding" changes. The transition from PEG chain helical structure to free coil structure is a key factor leading to the adsorption and desorption of BSA. Furthermore, the hydrophobic force of PS is the main driving force for BSA adsorption. These studies can inspire the design of amphiphilic materials to realize protein antifouling or transportation materials.
研究领域:
生物物理学
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材料科学
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蛋白质工程
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-9
·
IF 5.479
Yu Jiani,Yang Weitao,Xing Shige,Wang Jun,Han Huanxing,Zhang Pengfei,Xiang Chenyang,Zhang Bingbo
A fluorometric and magnetic resonance (MR) dual-modal detection scheme is presented for determination of ascorbic acid (AA). It is based on the use of a blended Au/MnO 2 @BSA mixture that was prepared via a biomimetic strategy, using bovine serum albumin (BSA) as the template at physiological temperature. The MnO 2 @BSA fraction (one part of the composite) is not susceptible to MR but can be degraded to MR-active compounds upon a redox reaction with even ultralow concentrations of AA. In parallel, the blended Au/MnO 2 @BSA recovers its fluorescence because MnO 2 @BSA acts as a quencher of the fluorescence of circumjacent Au@BSA (the other part of the composite). Fluorescence typically is measured at excitation/emission wavelengths of 470/625 nm. Leveraging on this redox reaction between MnO 2 and AA, a dual-mode detection scheme for AA was developed. Both the fluorescence and the MR signal increase with the concentration of AA. The lowest limit for the detection of AA is 0.6 μM in the fluorometric mode and 0.4 μM in the MR mode. Analysis of AA-spiked serum samples showed that the recoveries obtained by either the fluorometric and MR mode can reach 94%. This is the first report of the use of blended nanoparticles with their inherent cross-validation regularity.
Schematic presentation of the biomimetic synthesis of blended Au/MnO 2 @BSA nanoprobes and fluorometric/MR cross-validation dual-modal detection of ascorbic acid.
研究领域:
分析化学
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生物医学工程
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纳米材料科学
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DOI
PubMed
高影响因子
INTEGRATED FERROELECTRICS
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2021
·
IF 0.836
Ailing Yang,Zhikuang Dai
A green method was used to synthesize Cu2O@BSA nanoparticles (NPs). The influential factors on the morphologies of NPs were investigated. The result showed that the alkalinity of reactant solution plays an important role. Fourier transform infrared (FTIR) spectroscopy indicated that Cu2+ interacts strongly with O positioned at peptide chain and N in amide of BSA. The formation mechanism of Cu2O@BSA NPs depends on the unfolding of the peptide of BSA proceeds the electrostatic interaction between Cu2+ and BSA. The experimental results are of importance for fabrication of semiconductor NPs by using BSA or other protein as coating agents.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
ChemMedChem
·
2021
·Vol. 17
·期 3
·e202100570
·
IF 3.466
Yan Zhao,Jing-Jing Zhao,Jia-Xin Guo,Shuang-Qing Liu,Yang Li,Xiao-Yi Wang,Rong Li,Hui-Qin Tang,Zhen-Yu Li,Hui-Fen Yang,Bing Chen
Graphical Getting under the skin : BSA-CuS nanoparticles were used as photothermal reagents for depth photothermal therapy (PTT) treatment of subcutaneous abscesses. These nanoparticles exhibited remarkable photothermal conversion and low cytotoxicity combined with tissue-penetrating NIR laser (1064 nm) light. PTT based on BSA-CuS and 1064 nm NIR laser irradiation was found to sterilize drug-resistant pathogens in vitro and to eliminate subcutaneous abscesses in vivo effectively. That is OK, thank you! The treatment of subcutaneous abscess caused by drug-resistant bacteria is facing great difficulties and receiving more attention. In this work, we employed BSA-CuS nanoparticles as a photothermal reagent to apply photothermal therapy (PTT) to combat drug-resistant bacteria in vitro and subcutaneous abscess in vivo . The BSA-CuS nanoparticles were found to be stable and biocompatible without cytotoxicity toward NIH3T3 and 4T1 cells. In vitro experiments showed that three species of drug-resistant pathogens, including Escherichia coli , Staphylococcus aureus , and Candida albicans , could be effectively sterilized under co-incubation with BSA-CuS nanoparticles and then irradiation with 1064 nm NIR laser via tissue penetration. BSA-CuS nanoparticles together with 1064 nm NIR laser irradiation could also effectively diminish subcutaneous abscesses caused by drug-resistant bacteria on mice under PTT and depth PTT without causing any serious side effects and organic damage in vivo .That is OK, thank you!
研究领域:
医学
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生物材料学
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感染性疾病
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DOI
PubMed
高影响因子
CORROSION SCIENCE
·
2024
·
IF 7.4
Youqing Li,Ruiqing Hou,Pingli Jiang,Ke Li,Jun Wang,Di Mei,Lihong Wu,Shijie Zhu,Regine Willumeit-Rőmer,Shaokang Guan
Rapid degradation of magnesium (Mg) alloys and slow osteogenesis surrounding implants greatly limit their clinical applications. In present study, influence of BSA on the characteristics of CaCO 3 coating on MgZnCa alloy was investigated to adjust the degradation behaviors of Mg alloy and cell response to the coating. BSA can regulate the crystalline and the particle size of CaCO 3 in the coating and deteriorate coating stability and thickness, thereby leading to a tunable corrosion resistance for CaCO 3 @BSA coatings. Moreover, the presence of BSA bestows more suitable composition, roughness and wettability on cells with a better osteogenic performance on the coating.
研究领域:
材料科学
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生物医学工程
·
骨科
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DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 27
·19284-19293
·
IF 3.9
Tao Li,Zhuo Li,Fengjiao Chen,Liying Zhu,Hua Tang,Dan Wang,Zhenrong Tang
Bovine serum albumin-stabilized Au nanoclusters (BSA-Au NCs) have emerged as promising contenders for imaging agents and highly sensitive fluorescence sensors due to their biocompatibility and strong photoluminescence. Optimizing the synthesis conditions of BSA-Au NCs is crucial for enhancing fluorescence imaging and other nanocluster applications. In this study, for the first time, we systematically investigated the effects of BSA concentration and Au3+ on both particle size and optical characteristics of BSA-Au NCs. When the two components achieved a suitable concentration ratio, it was beneficial to form BSA-Au NCs with a high quantum yield (QY = 74.30%) and good fluorescence stability. In contrast, an inappropriate concentration ratio would lead to the formation of gold nanoparticles (Au NPs), and their internal filtration effect (IFE) would attenuate the fluorescence emission of BSA-Au NCs. The BSA-Au NCs were then employed as efficient fluorescence sensors for detecting Hg2+. Furthermore, the growth mechanism of BSA-Au NCs was elucidated by monitoring fluorescence changes during different incubation times. The BSA-Au NCs with a high quantum yield introduce a novel synthetic concept for sensitive fluorescent probes and expanding versatile applications of BSA-Au NCs in catalysis, chemical sensing and biomedicine.
研究领域:
纳米技术
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材料科学
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
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