Caifeng Ding,Yujuan Xu,Yanan Zhao,Hua Zhong,Xiliang Luo
Drug delivery which can offer efficient and localized drug transportation together with imaging capabilities is highly demanded in the development of cancer theranostic approaches. Herein, we report the construction of bovine serum albumin (BSA) gold nanoclusters (BSA@AuNCs) for cell fluoresce imaging and target drug delivery. BSA@AuNCs were modified with cyclic arginine-glycine-aspartate with the product RGD–BSA@AuNCs to enhance cell internalization of the nanoclusters. Furthermore, doxorubicin hydrochloride or doxorubicin (DOX), a widely used chemotherapy drug, was also used to modify RGD–BSA@AuNCs. The final design of the DOX/RGD–BSA@AuNC system was constructed through the disulfide bond. The physical microstructure and biological characterization of the BSA@AuNCs were realized through high-resolution transmission electron microscopy and confocal laser fluorescence microscopy. As the disulfide bonds were cleaved by glutathione in cancer cells, DOX-SH molecules were released from the nanosystem to inhibit the growth of cancer cells. The as-prepared DOX/RGD–BSA@AuNC system can be used not only to deliver drug but also to achieve the antitumor effect by in vivo imaging, demonstrating its promising applications in cancer treatment.
Herein we present the development of a bovine serum albumin (BSA)-coated fluorescent gold nanocluster (BSA-AuNC)/graphene oxide (GO) hybrid nanocomposite-based photoelectrochemical (PEC) biosensor, which can be used for multichannel detection of both hydrogen peroxide (H 2 O 2 ) and dopamine (DA) within one chip at negative and positive potentials, respectively. BSA-AuNC-based biosensors generally exhibit poor PEC sensing performance owing to their poor separation of photoexcited electron-hole pairs and low electrode coverage. Consequently, improved AuNC/GO hybrid nanocomposites and a layer-by-layer (LBL) method are introduced in this study. The enhancement was investigated by exploring the optimum concentration of GO (0.12 mg mL −1 ) and LBL number (four layers) for the preparation of the BSA-AuNC materials, revealing that GO plays different enhancement roles in the detection of H 2 O 2 and DA. Compared to the results from other gold nanoclusters with different ligands, the photoelectrical enhancement of BSA-AuNCs can be achieved for both negative and positive potentials simultaneously with the same optimum GO concentration and LBL number. Thus, the detection of H 2 O 2 and DA was achieved by BSA-AuNC/GO multilayers with enhanced sensing properties and easier fabrication processes. The two enhancement methods acting together led to an improvement in the limits of detection (LODs) from 325 μM to 23 μM (H 2 O 2 ) and from 7.45 μM to 1.5 μM (DA). Thus, our strategy shows great potential for the improvement of PEC sensing structure, and provides a method for multichannel detection.
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