Science Advances
·
2023
·Vol. 9
·期 1
·
IF 13.6
Zipeng Yu,Jinxin Ma,Mengyue Zhang,Xiaoxuan Li,Yi Sun,Mengxin Zhang,Zhaojun Ding
Auxin and brassinosteroids (BRs) are two major growth-promoting phytohormones that shape hypocotyl elongation; however, the cross-talk between auxin and BR in this process is not fully understood. In this study, we found that auxin-induced hypocotyl elongation is dependent on brassinazole-resistant 1 (BZR1), a core BR signaling component. Auxin promotes BZR1 nuclear accumulation in hypocotyl cells, a process dependent on mitogen-activated protein kinase 3 (MPK3) and MPK6, which are both activated by auxin and whose encoding genes are highly expressed in hypocotyls. We determined that MPK3/MPK6 phosphorylate and reduce the protein stability of general regulatory factor 4 (GRF4), a member of the 14-3-3 family of proteins that retain BZR1 in the cytoplasm. In summary, this study reveals the molecular mechanism by which auxin promotes hypocotyl elongation by enhancing BZR1 nuclear accumulation via MPK3/MPK6-regulated GRF4 protein stability.
研究领域:
植物生物学
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分子生物学
·
激素信号
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DOI
PubMed
高影响因子
PLANT CELL TISSUE AND ORGAN CULTURE
·
2023
·
IF 3
Zhang Ting,Zhao Yichen
Tea plant ( Camellia sinensis (L.) O. Kuntze) is an important woody economic crop that has been cultivated worldwide. Adversity stress acts as a significant factor affecting the growth and development of tea plants. Brassinazole-resistant (BZR) family protein is an essential factor in regulating plant stress resistance and plays a key role in cold stress response, heat, drought and salt resistances. In this study, the promoter region upstream of CsBZR1-2 was cloned. The promoter sequence and its 5′-end truncated segment were inserted upstream of β-glucuronidase ( GUS ) gene in the pCambia1391Z plasmid. Stably expressed transgenic tobacco lines were obtained by Agrobacterium tumefaciens -mediated genetic transformation. The results showed that except for promoter lacking the core element, both full-length and truncated promoters could initiate the expression of GUS , but the level of expression was affected by the degree of deletion at the 5′-end. The core region of the promoter was in a 311 bp region including the transcription start site (TSS). In addition, the regulatory mechanism of the promoter was explored. It was found that the genes regulated by the promoter were responsive to stress such as cis-acting elements (MeJA, ABRE and LTR), BR and light. This paper provides a theoretical basis to further explore the regulatory mode of the promoter and to achieve efficient gene expression.
研究领域:
植物生物学
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分子遗传学
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逆境生理学
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产品关联
DOI
高影响因子
PLANT JOURNAL
·
2020
·Vol. 102
·期 5
·931-947
·
IF 6.141
Meng-Yu Yan,Dong-Ling Xie,Jia-Jian Cao,Xiao-Jian Xia,Kai Shi,Yan-Hong Zhou,Jie Zhou,Christine H. Foyer,Jing-Quan Yu
Summary Phytohormone brassinosteroids (BRs) are essential for plant growth and development, but the mechanisms of BR-mediated pollen development remain largely unknown. In this study, we show that pollen viability, pollen germination and seed number decreased in the BR-deficient mutant d ^im , which has a lesion in the BR biosynthetic gene DWARF ( DWF ), and in the bzr1 mutant, which is deficient in BR signaling regulator BRASSINAZOLE RESISTANT 1 ( BZR1 ), compared with those in wild-type plants, whereas plants overexpressing DWF or BZR1 exhibited the opposite effects. Loss or gain of function in the DWF or BZR1 genes altered the timing of reactive oxygen species (ROS) production and programmed cell death (PCD) in tapetal cells, resulting in delayed or premature tapetal degeneration, respectively. Further analysis revealed that BZR1 could directly bind to the promoter of RESPIRATORY BURST OXIDASE HOMOLOG 1 ( RBOH1 ), and that RBOH1 -mediated ROS promote pollen and seed development by triggering PCD and tapetal cell degradation. In contrast, the suppression of RBOH1 compromised BR signaling-mediated ROS production and pollen development. These findings provide strong evidence that BZR1-dependent ROS production plays a critical role in the BR-mediated regulation of tapetal cell degeneration and pollen development in Solanum lycopersicum (tomato) plants.
研究领域:
植物生物学
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分子生物学
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发育生物学
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激素信号传导
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DOI
PubMed
高影响因子
JOURNAL OF EXPERIMENTAL BOTANY
·
2021
·Vol. 72
·期 13
·4721-4743
·
IF 6.992
Xiao Shenghua,Hu Qin,Zhang Xiaojun,Si Huan,Liu Shiming,Chen Lin,Chen Kun,Berne Sabina,Yuan Daojun,Lindsey Keith,Zhang Xianlong,Zhu Longfu
Salicylic acid (SA) and brassinosteroids (BRs) are well known to regulate diverse processes of plant development and stress responses, but the mechanisms by which these phytohormones mediate the growth and defense trade-off are largely unclear. In addition, little is known about the roles of DEHYDRATION RESPONSIVE ELEMENT BINDING transcription factors, especially in biotic stress and plant growth. Here, we identified a cotton (Gossypium hirsutum) APETALA2/ETHYLENE RESPONSIVE FACTOR gene GhTINY2 that is strongly induced by Verticillium dahliae. Overexpression of GhTINY2 in cotton and Arabidopsis enhanced tolerance to V. dahliae, while knockdown of expression increased the susceptibility of cotton to the pathogen. GhTINY2 was found to promote SA accumulation and SA signaling transduction by directly activating expression of WRKY51. Moreover, GhTINY2-overexpressing cotton and Arabidopsis showed retardation of growth, increased sensitivity to inhibitors of BR biosynthesis, down-regulation of several BR-induced genes, and up-regulation of BR-repressed genes, while GhTINY2-RNAi cotton showed the opposite effects. We further determined that GhTINY2 negatively regulates BR signaling by interacting with BRASSINAZOLE-RESISTANT 1 (BZR1) and restraining its transcriptional activation of the expression of INDOLE-3-ACETIC ACID INDUCIBLE 19 (IAA19). These findings indicate that GhTINY2 fine-tunes the trade-off between immunity and growth via indirect crosstalk between WRKY51-mediated SA biosynthesis and BZR1-IAA19-regulated BR signaling.
研究领域:
植物生物学
·
分子生物学
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植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）