SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Hongmei Zou,Mingxuan Zhang,Jin Chen,Stanley Aniagu,Yan Jiang,Tao Chen
Benzo[ a ]pyrene (BaP) is a representative polycyclic aromatic hydrocarbon widely present in the environment. We previously reported that the aryl hydrocarbon receptor (AHR) mediates BaP-induced apoptosis and cardiac malformations in zebrafish embryos, but the underlying molecular mechanisms were unclear. Since BaP is a mutagenetic compound, we hypothesize that BaP induces apoptosis and heart defects via AHR-mediated DNA damage. In this study, zebrafish embryos were exposed to BaP at a concentration of 0.1 μM from 2 to 72 h post fertilization, either with or without inhibitors/agonists. AHR activity and levels of reactive oxygen species (ROS) were examined under a fluorescence microscope. mRNA expression levels were quantified by qPCR. DNA damage and apoptosis were detected by immunofluorescence. Our findings revealed that BaP exposure significantly increased BPDE-DNA adducts, mitochondrial damage, apoptosis and heart defects in zebrafish embryos. These effects were counteracted by inhibiting AHR/cyp1a1 using pharmaceutical inhibitors or genetic knockdown. Furthermore, we observed that spironolactone, an antagonist of nucleotide excision repair (NER), significantly enhanced BaP-induced BPDE-DNA adducts, mitochondrial damage, apoptosis and heart malformation rates. Conversely, SRT1720, a SIRT1 agonist, reduced the adverse effects of BaP. Supplementation with spironolactone also enhanced γ-H2AX signals in the heart of zebrafish embryos exposed to BaP. Additional experiments demonstrated that BaP suppressed the expression of SIRT1. We further established that AHR, when activated by BaP, directly inhibited SIRT1 transcription, leading to downregulation of XPC and XPA, which are essential NER genes involved in the recognition and verification steps of the NER process. Taken together, our results indicate that AHR mediates BaP-induced DNA damage in the heart of zebrafish embryos by inducing BPDE-DNA adduct formation via the AHR/Cyp1a1 signalling pathway, as well as suppressing NER via AHR-mediated inhibition of SIRT1.
研究领域:
毒理学
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发育生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Xin-Jun Yu,Hong Chen,Chang-Yi Huang,Xiao-Yu Zhu,Zhi-Peng Wang,Dong-Sheng Wang,Xiao-Yan Liu,Jie Sun,Jian-Yong Zheng,Hui-Juan Li,Zhao Wang
The phytohormone 6-benzylaminopurine (6-BAP) significantly improves lipid synthesis of oleaginous microorganisms with the great potential applied in lipid production. In the current study, the lipid and DHA productions in oleaginous Aurantiochytrium sp. were found to be improved by 48.7% and 55.3%, respectively, induced by 6-BAP treatments. Then, using high-throughput RNA-seq technology, the overall de novo assembly of the cDNA sequence data generated 53871 unigenes, and 15902 of these were annotated in at least one database. The comparative transcriptomic profiles of cells with and without 6-BAP treatments revealed that a total of 717 were differently expressed genes (DE), with 472 upregulated and 245 downregulated. Further annotation and categorization indicated that some DE genes were involved in pathways crucial to lipid and DHA productions, such as fatty acid synthesis, central carbon metabolism, transcriptional factor, signal transduction, and mevalonate pathway. A regulation mode of 6-BAP, in turn, perception and transduction of 6-BAP signal, transcription factor, expression regulations of the downstream genes, and metabolic changes, respectively, was put forward for the first time in the present study. This research illuminates the transcriptomic mechanism of phytohormone stimulation of lipid and DHA production in an oleaginous microorganism and provides the potential targets modified using genetic engineering for improving lipid and DHA productivity.
研究领域:
微生物学
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分子生物学
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生物技术
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转录组学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yinxiu Liang,Min Ji,Hongyan Zhai,Jun Zhao
Bioanodes in a soil microbial fuel cell (SMFC) can serve as sustainable electron acceptors in microbial metabolism processes; thus, SMFCs are considered a promising in situ bioremediation technology . Most related studies have focused on the removal efficiency of contaminants. Relatively few efforts have been made to comprehensively investigate the organic matter composition and biodegradation metabolites of organic contaminants and microbial communities at various distances from the bioanode. In this study, the level and composition of dissolved organic matter (DOM), biodegradation metabolites of benzo[ a ]pyrene (BaP), and microbial communities at two sites with different distances (S 1cm and S 11cm ) to the bioanode were investigated in an SMFC. The consumption efficiency of dissolved organic carbon (R DOC ) and removal efficiency of BaP (R BaP ) at S 1cm were slightly higher than those at S 11cm after 100 days (R DOC 47.82 ± 5.77% at S 1cm and 44.98 ± 10.76% at S 11cm ; R BaP 72.52 ± 1.88% at S 1cm and 68.50 ± 4.34% at S 11cm ). More fulvic acid-like components and more low-molecular-weight metabolites (indicating a higher biodegradation degree) of BaP were generated at S 1cm than at S 11cm . The microbial community structures were similar at the two sites. Electroactive bacteria (EAB) and some polycyclic aromatic hydrocarbon degraders were both enriched at the bioanode. Energy metabolism at the bioanode could be upregulated to generate more adenosine triphosphate (ATP). In conclusion, the bioanode could modulate the metabolic pathways in the adjacent soil by strengthening the contact between the EAB and BaP degraders, and providing more ATP to the BaP degraders.
研究领域:
环境科学
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微生物学
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生物修复
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土壤学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2020
·
IF 5.774
Yinxiu Liang,Min Ji,Hongyan Zhai,Rumeng Wang
A novel closed-circuit permeable electroactive well (CPEW) was constructed to remove benzo[a]pyrene (BaP) from soil. BaP was removed sequentially through interception, adsorption and further biodegradation on a graphite felt, which acted as a filtration layer and also a bioanode. The removal efficiency of absolute BaP amount in CPEW was 52.52 ± 4.23%, higher than 44.94 ± 2.69% in the control (open-circuit PEW, OPEW). Physical adsorption and biodegradation accounted for 66.31 ± 4.15% and 33.51 ± 1.58% of BaP removed from soil in CPEW, and 86.26 ± 5.21% and 13.57 ± 1.29% in OPEW at 50 d. Bioelectrochemical process greatly enhanced the biodegradation efficiency of absolute BaP amount from 6.13 ± 0.49% (OPEW) to 17.63 ± 1.49% (CPEW). High abundances of Gammaproteobacteria and Clostridia on the anode of CPEW could significantly enhance electrons transfer and thus improve BaP biodegradation. Moreover, Vibrionaceae (belonged to class Gammaproteobacteria ) possibly played an important role in electron transfer. Based on the function predication analysis, the bioelectrochemical process could enhance the BaP biodegradation through increasing metabolic rates instead of changing metabolic pathways. Conclusively, CPEW, efficiently integrating filtration, adsorption and bioelectrochemical degradation, is a promising easy-to-implement in situ remediation technology for soil, sediment or groundwater contaminated with refractory organics.
研究领域:
环境科学
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微生物学
·
污染治理
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 36
·85558-85568
·
IF 5.8
Jin
Jingnan,Shi Yahui,Zhang Baozhong,Wan Dongjin,Zhang Qingye
A bacterial strain Citrobacter sp. HJS-1 was discovered from the sludge in a drainage canal of a coal mine. Firstly, its biodegradation capacity for benzo[a]pyrene (BaP) was detected under different concentrations. The results proved that the strain possessed excellent biodegradation capacity for BaP with high-efficiency degradation rates ranging from 78.9 to 86.8%. The highest degradation rate was observed in the low-concentration sample, and the high-concentration BaP had a slight influence on the biodegradation capacity due to the potential toxicity of BaP and its oxygen-containing derivatives. Meanwhile, the degradation test for the other five aromatic hydrocarbons (2- to 4-ring) proved that the strain had a comprehensive degradation potential. To clarify the biodegradation mechanism of BaP, a dioxygenase structure was constructed by homology modeling. Then, the interactions between dioxygenase and BaP were researched by molecular simulation. Combined with the identification of the vital BaP-cis-7,8-dihydrodiol intermediate and the interaction analysis, the initial oxidation mode and the binding site of BaP were revealed in the dioxygenase. Taken together, this study has offered a way to understand the biodegradation process of BaP and its interaction mechanism based on experimental and theoretical analysis.
研究领域:
环境微生物学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2026
·Vol. 237
·期 22
·1293
·
IF 3.5
Li Xi,Zhang Luyao,Liu Ling,Yan Yunxia,Wang Ziqing,Wang Bing
Soil co-contamination by organic pollutants and toxic metals has become an increasingly prominent environmental concern worldwide, largely driven by intensive industrial and anthropogenic activities. In this study, BC-nZVI coupled with three oxidants—persulfate (PS), BS, and hydrogen peroxide (HP)—was evaluated for the simultaneous remediation of BaP and Cr(VI) co-contaminated soil. Among the tested systems, BC-nZVI/BS exhibited the best overall performance, achieving 77.7% BaP removal and 97.6% Cr(VI) reduction, followed by BC-nZVI/HP and BC-nZVI/PS. Among the three systems, the BC-nZVI/BS system maintained comparatively stable remediation performance over the tested pH range. Coexisting Cr(VI) exerted a pronounced synergistic effect on BaP degradation in the BC-nZVI/BS system, likely via promoting oxidant activation. Six classes of intermediates with simpler structures than BaP were tentatively identified, indicating the progressive transformation of BaP during the remediation process. Post-treatment characterization revealed the presence of Cr 2 O 3 , FeCr 2 O 4 , and Cr(OH) 3 , suggesting that Cr(VI) was predominantly removed through reductive precipitation to Cr(OH) 3 . Overall, the BC-nZVI/BS system showed promising potential for the remediation of BaP-Cr(VI) co-contaminated soils.
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Dawen Gao,Guanyu Chen,Zelin Hou,Huayu Tao,Xuran Du,Hong Liang
Immobilized crude enzyme with sorption-modified bentonite presents a novel bioremediation strategy for recalcitrant polycyclic aromatic hydrocarbons (PAHs) in contaminated soils. However, the microbial community dynamics under PAH stressors remain unclear. Through a 30-day microcosm experiment using in situ contaminated soil, we systematically evaluated: (i) PAHs removal efficiency, (ii) dissolved organic matter (DOM) fractionation, and (iii) microbiome restructuring across three treatments: natural attenuation with contaminated soil (CK), saponin modified bentonite with contaminated soil (Sap-BT), and immobilized crude enzyme with modified bentonite with contaminated soil (CE-Sap-BT). The results showing that the addition of modified bentonites reached 52.45 % removal rate of Benzo[ a ]pyrene. Increased protein-like component (C1) and two humic-like components (C2 and C3) by 6.69 %, 12.57 % and 18.43 % compared to CK, while immobilized crude enzyme with sorption-modified bentonite enhanced the aerobic function of PAH-degrading microorganisms. Co-occurrence network analysis revealed the strong linkages of soil properties and key bacterial taxa. These results establish that immobilized crude enzyme with sorption-modified bentonite application coupling drives functional microbiome selection, providing novel insights into optimizing in situ PAH remediation protocols.
研究领域:
环境科学
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土壤生物学
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微生物生态学
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Fei Li,Lei Zhu,Ling Chen,Yong Min,Yimin Qiu,Xiaoyan Liu
The two-spotted spider mite ( Tetranychus urticae ) is a major pest that causes severe agricultural losses. Its resistance to chemical acaricides diminishes their effectiveness, exacerbating their misuse and environmental pollution, highlighting the need for alternative solutions. This study presented a biomimetic nano silica acaricide (BNSA) developed using a fusion protein, BAP-R5, which integrated the acaricidal protein BAP from Bacillus vallismortis NBIF-001 with the R5 peptide from Cylindrotheca fusiformis . Through ecofriendly biomimetic silicification, the BAP-R5 protein achieved high encapsulation efficiency (91.2%-98.0%) within nano silica matrices. The resulting BNSAs featured tunable micromorphologies from dispersed nanoparticles to dense wet gels. The formulation sustained protein release in 14 days with release rates ranging from 12.5% to 21.0%, and robust adhesion to both mite cuticles and fava bean leaves. Notably, the BNSAs exhibited 2.45-fold higher contact toxicity against T. urticae compared to free BAP-R5, confirming synergistic effects of nanoencapsulation. Under abiotic stress (pH 2-12, 60-80 °C), the BNSAs retained 67.3%-93.9% residual activity versus 38.2%-71.5% for free protein, validating their environmental resilience. These results establish a novel paradigm for precision-targeted nano pesticide design through biomimetic engineering, addressing critical challenges in sustainable agriculture.
研究领域:
农业科学
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生物技术
·
害虫防治
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Jing Chen,Xiu He,Yawen Song,Ying Tu,Wenyan Chen,Guanghong Yang
Dibutyl phthalate (DBP) and Benzo(a)pyrene (BaP) are ubiquitous contaminants in environment and foodstuffs, which increase the chance of their combined exposure to humans in daily life. However, the combined effects of DBP and BaP on liver and the underlying mechanisms are still unclear. In this study, we explored the combined effects of DBP and BaP on liver and the potential mechanisms in a rat model. We found that DBP and BaP co-exposure activated the MyD88/NF-κB pathway through increasing TLR4 acetylation (TLR4ac) level, leading to the imbalance of pro-inflammatory factors (CXCL-13, IL-6 and TNF-α) and anti-inflammatory factors (IL-10), ultimately resulting in liver tissue damage and functional changes. Sporoderm-broken spores of Ganoderma lucidum (SSGL) had strong alleviating effects on liver injury induced by DBP and BaP co-exposure. Our study found that SSGL suppressed TLR4ac-regulated MyD88/NF-κB signaling to reduce the release of pro-inflammatory factors, and promote the secretion of IL-10, thus alleviating liver injury caused by DBP and BaP co-exposure. In conclusion, SSGL contributed to liver protection against DBP and BaP-induced liver injury in rats via suppressing the TLR4ac-regulated MyD88/NF-κB signaling.
研究领域:
毒理学
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药理学
·
分子生物学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Xiao-min Zhang,Chao-yang Xu,Teng Hui,Ke-zhou Cai,Hui Zhou,Cong-gui Chen,Bao-cai Xu
Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that has been found in barbecued meat products and has shown carcinogenic properties in humans. The purpose of this study was to investigate the effect of vinegar (fermented vinegars and distilled vinegar) on the generation of BaP in barbecued pork sausages. The results showed that fermented vinegars and distilled vinegar could significantly ( P < 0.05) inhibit the formation of BaP (26.8%–82.3%) in barbecued pork sausages. Furthermore, fermented vinegar could enhance the sensory evaluation of barbecued pork sausages(tenderness, flavor, juiciness and overall acceptability). Additionally, an intermediate of the Maillard reaction (hydroxymethylfurfural) (0.14–2.34 mg/kg), which is possibly associated with BaP generation, was inhibited because of the decrease in pH. The fermented vinegars, which were rich in phenols (1787.09–3089.60 mg GRE/L) and flavonoid (622.54–2167.97 mg RE/L) compounds, inhibited the formation of BaP by scavenging reactive oxide species (1,1-diphenyl-2-picrylhydrazine (DPPH) radical (85.50–96.73%) and hydroxy free radical (34.56–66.30%) scavenging activities) in pork sausages. Therefore, the vinegars inhibited the content of BaP in barbecued pork sausages by decreasing the pH and scavenging free radicals. In addition, ten phenolic compounds in sun vinegar were confirmed to be critical in inhibiting the content of BaP in barbecued pork sausages. This study provides a more affordable and feasible strategy for the inhibition of BaP in meat processing.
研究领域:
食品科学
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毒理学
·
化学
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产品关联
DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 27
·18878-18887
·
IF 3.9
Jingnan Jin,Yahui Shi,Baozhong Zhang,Dongjin Wan,Qingye Zhang,Ying Li
Owing to its adverse effects on the environment and human health, benzo[a]pyrene (BaP) has attracted considerable attention and has been used as a model compound in ecotoxicology. In this study, Pannonibacter sp. JPA3 as a BaP-degrading strain was isolated from the production water of an oil well. The strain could remove 80% of BaP at an initial concentration of 100 mg L−1 after 35 d culture. The BaP-4,5-dihydrodiol, BaP-4,5-epoxide, 5-hydroxychrysene, and 2-hydroxy-1-naphthoic acid metabolites were identified in the biodegradation process. Simultaneously, the gene sequence coding for dioxygenase in the strain was amplified and a dioxygenase model was built by homology modeling. Combined with the identification of the metabolites, the interaction mechanism of BaP with dioxygenase was investigated using molecular docking. It was assumed that BaP was initially oxidized at the C4–C5 positions in the active cavity of dioxygenase. Moreover, a hypothesis for the progressive degradation mechanism of BaP by this strain was proposed via the identification of the downstream metabolites. In conclusion, our study provided an efficient BaP degrader and a comprehensive reference for the study of the degradation mechanism in terms of the degrading metabolites and theoretical research at the molecular level.
研究领域:
环境科学
·
微生物学
·
生态毒理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
TOXICOLOGY
·
2021
·
IF 4.221
Yujie Huang,Jie Zhang,Yizhou Tao,Cheng Ji,Stanley Aniagu,Yan Jiang,Tao Chen
Benzo[a]pyrene (BaP), a prototypical polycyclic aromatic hydrocarbon, is widely present in the environment. BaP-induced heart defects have been frequently reported, but the underlying molecular mechanisms remain elusive. Here, we found that BaP increased heart malformations in zebrafish embryos in a concentration-dependent manner, which were attenuated by supplementation with either CH223191 (CH), an aryl hydrocarbon receptor (AHR) inhibitor, or N-acetyl- l -cysteine (NAC), a reactive oxygen species (ROS) scavenger. While CH and NAC both inhibited BaP-induced ROS generation, NAC had no effect on BaP-induced AHR activation. We further demonstrated that BaP increased mitochondrial ROS, decreased mitochondrial membrane potential, and caused endogenous apoptosis, with all these effects being counteracted by supplementation with either CH or NAC. Resveratrol (RSV), a natural AHR antagonist and ROS scavenger, also counteracted the heart malformations caused by BaP. Further experiments showed that RSV attenuated BaP-induced oxidative stress, mitochondrial damage and apoptosis, but had no significant effect on AHR activation. In conclusion, our findings show that BaP induces oxidative stress via AHR activation, which causes mitochondria-mediated intrinsic apoptosis, resulting in heart malformations in zebrafish embryos, and that RSV had a protective effect against BaP-induced heart defects mainly by inhibiting oxidative stress rather than through antagonism of AHR activity.
研究领域:
环境毒理学
·
心血管发育
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）