CATALYSIS COMMUNICATIONS
·
2014
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IF 3.32
Jian-yong Zheng,Yu-qiang Wang,Wei-feng Luo,Sha-sha Zhou,Qing Zhu,Xiang-xian Ying,Zhao Wang
A new esterase-producing strain ( Bacillus amyloliquefaciens WZZ002) that exhibits high hydrolytic activity, excellent enantioselectivity, and high substrate tolerance on Boc- dl -Alanine methyl ester was isolated from soil samples. The reaction temperature, pH, and neutralizer optima of the cell-mediated biocatalysis were 35 °C, pH 8.0, and NH 3 ·H 2 O, respectively. The optimal substrate concentration was 2 M, with a biocatalyst loading of 50 g/L. Results showed that the enantiomeric excess values of substrate and product were both greater than 99%. Thus, bioprocessing with the use of the isolated strain is a promising route for the commercial production of Boc- d -Ala-OMe.
研究领域:
生物化学
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微生物学
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制药工程
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产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2020
·Vol. 44
·期 4
·727-735
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IF 2.419
Wang Hongrao,Han Shuran,Wang Jiayi,Yu Shuyu,Li Xiaoyan,Lu Lei
Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during the synthesis of M-CLEAs. With ammonium sulfate as precipitant, maximum activity was recovered by cross-linking with 0.16% glutaraldehyde for 1 h. The prepared M-CLEAs exhibited improved activity under alkaline conditions. It remained 74% activity after incubation at 60 °C for 5 h. Enhanced tolerance towards NaCl was also observed for the M-CLEAs, with 68% activity remaining in the presence of 1 M NaCl. The immobilized laccase could rapidly decolorize more than 93% of reactive black 5 and indigo carmine in 1 h, while its catalytic efficiency towards reactive blue 19 was relatively low. After four cycles of consecutive reuse, the M-CLEAs could decolorize 92% of indigo carmine. The easy recovery and reusability of M-CLEAs facilitate the potential application of bacterial laccase in dye decolorization.
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Hongxuan Li,Yubo Zhang,Yaping Zheng,Xuejian Li,Zimu Li,Chaoxin Man,Yu Zhang,Yujun Jiang
The probiotic strain Bacillus amyloliquefaciens JM033 ( B. amyloliquefaciens JM033), isolated from the traditional Chinese fermented food Sufu (also known as Fu-ru or fermented bean curd), is distinguished by its high production of exopolysaccharides (EPS). The EPS (BAP-1) produced by this strain was purified and its structure analyzed. BAP-1 is a novel hybrid fructan with a molecular weight of 17.6 kDa. It is composed of →6)- β -D-Fru f -(2 → and →1,6)- β -D-Fru f -(2→, which form the backbone, with a branched chain of β -D-Fru f -(2 → attached at the 1-position of residue B. In vivo studies on mice indicated that BAP-1 improves immunity in immunosuppressed mice by enhancing humoral immunity ( P < 0.01), monocyte-macrophage phagocytosis ( P < 0.01), and NK cell killing activity ( P < 0.05). Additionally, BAP-1 was found to improve the composition of the intestinal microbiota and stimulate the production of short-chain fatty acids. Notably, BAP-1 exhibited a significant effect on the proliferation of Akkermansia . Therefore, BAP-1 shows promise as a prebiotic and may contribute to the development of new immunomodulatory agents.
研究领域:
微生物学
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免疫学
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营养学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2016
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IF 2.667
Waseem Raza,Zhong Wei,Ning Ling,Qiwei Huang,Qirong Shen
Three organic fertilizers made of different animal and plant waste materials (BOFs) were evaluated for their effects on the production of antibacterial volatile organic compounds (VOCs) by two Bacillus amyloliquefaciens strains SQR-9 and T-5 against the tomato wilt pathogen Ralstonia solanacearum (RS). Both strains could produce VOCs that inhibited the growth and virulence traits of RS; however, in the presence of BOFs, the production of antibacterial VOCs was significantly increased. The maximum inhibition of growth and virulence traits of RS by VOCs of T-5 and SQR-9 was determined at 1.5% BOF2 and 2% BOF3, respectively. In case of strain T-5, 2-nonanone, nonanal, xylene, benzothiazole, and butylated hydroxy toluene and in case of strain SQR-9, 2-nonanone, nonanal, xylene and 2-undecanone were the main antibacterial VOCs whose production was increased in the presence of BOFs. The results of this study reveal another significance of using organic fertilizers to improve the antagonistic activity of biocontrol agents against phytopathogens.
研究领域:
微生物学
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植物病理学
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DOI
PubMed
高影响因子
Systems Microbiology and Biomanufacturing
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2023
·
IF 0
Li Chang-Long,Li Meng,Zhang Wei-Guo,Xu Jian-Zhong
Menaquinone-7 (MK-7) has an important role in preventing diseases such as cardiovascular disease and osteoporosis. In this study, a combination strategy of strain improvement and medium optimization is investigated to increase MK-7 production in Bacillus amyloliquefaciens . Conventional breeding method was first used to modify the biosynthetic pathway to construct a MK-7 high-producing strain by atmospheric and room temperature plasma mutagenesis and protoplast fusion. The resulted strain Ba-4 with resistance to sulfaguanidine, 1-hydroxy-2-naphthoic acid, menadione, 2-deoxy- d -glucose and rifampicin as well as sensitive to β-fluoropyruvate produced 73.57 ± 1.61 mg/L of MK-7, which was 1.36 times more than that of the parent strain H.β.D.R.-5 (i.e., 31.12 ± 1.40 mg/L). Subsequently, single-factor optimization and response surface methodology (RSM) were used to optimize the medium components for increasing MK-7 production by strain Ba-4. Strain Ba-4 produced 90.43 ± 1.32 mg/L of MK-7 under the single-factor optimized medium. Moreover, the results of response surface methodology indicated that glycerol, soy peptone and Tween-80 had significant effects on MK-7 production, and the highest MK-7 production (i.e., 95.03 ± 1.01 mg/L) was obtained under the optimized medium, which was 0.29 times higher than that of the initial medium. These results confirmed that the conventional breeding methods and fermenter control system are effective strategies in improving MK-7 production by B. amyloliquefaciens .
研究领域:
微生物学
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生物技术
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发酵工程
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DOI
Scientific Reports
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2016
·Vol. 6
·期 1
·1-13
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IF 5.228
Raza Waseem,Ling Ning,Yang Liudong,Huang Qiwei,Shen Qirong
It is important to study the response of plant pathogens to the antibiosis traits of biocontrol microbes to design the efficient biocontrol strategies. In this study, we evaluated the role of volatile organic compounds (VOCs) produced by a biocontrol strain Bacillus amyloliquefaciens SQR-9 on the growth and virulence traits of tomato wilt pathogen Ralstonia solanacearum (RS). The VOCs of SQR-9 significantly inhibited the growth of RS on agar medium and in soil. In addition, the VOCs significantly inhibited the motility traits, production of antioxidant enzymes and exopolysaccharides, biofilm formation and tomato root colonization by RS. The strain SQR-9 produced 22 VOCs, but only nine VOCs showed 1–11% antibacterial activity against RS in their corresponding amounts; however, the consortium of all VOCs showed 70% growth inhibition of RS. The proteomics analysis showed that the VOCs of SQR-9 downregulated RS proteins related to the antioxidant activity, virulence, carbohydrate and amino acid metabolism, protein folding and translation, while the proteins involved in the ABC transporter system, amino acid synthesis, detoxification of aldehydes and ketones, methylation, protein translation and folding, and energy transfer were upregulated. This study describes the significance and effectiveness of VOCs produced by a biocontrol strain against tomato wilt pathogen.
研究领域:
植物病理学
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微生物学
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农业科学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2017
·
IF 5.651
Cai Hui,Xiaoxiao Guo,Pengfei Sun,Rashid Azim Khan,Qichun Zhang,Yongchao Liang,Yu-Hua Zhao
A newly verified adsorbent biofilm produced by Bacillus amyloliquefaciens DT was investigated for nitrite removal from aqueous solutions. The biofilm’s characteristics and adsorption mechanism were determined, with results indicating that nitrite ions were adsorbed onto the protonated amine sites of biofilm under acidic conditions. Analysis of various factors showed that higher nitrite adsorption capacities occurred at pH < 3.0 and higher temperatures as well as higher initial nitrite concentrations, with a maximum nitrite removal capacity of 116.84 mg/g. Furthermore, nitrite adsorption was well fitted to the pseudo second-order and Weber–Morris kinetic models, and the Freundlich and Sips isotherm models. Simultaneously, thermodynamic analysis demonstrated that nitrite adsorption is a spontaneous endothermic process. In summary, the adsorption of nitrite was complex, and mainly resulted from electrostatic attraction and intraparticle diffusion. Consequently, the B. amyloliquefaciens biofilm can be considered as a promising adsorbent for nitrite removal from wastewater.
研究领域:
环境科学
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微生物学
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水处理
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吸附技术
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DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2020
·
IF 3.448
Zelong Li,Runtao Zhu,Yongqi Liu,Jiaqi Li,Haofeng Gao,Nan Hu
L-theanine, a unique amino acid in green tea with health benefits, can be enzymatically synthesized by γ-glutamyltranspeptidase (γ-GT; EC 2.3.2.2). Here, a salt-tolerant γ-glutamyltranspeptidase from a marine bacterium Bacillus amyloliquefaciens was expressed in Escherichia. coli BL21 (DE3) and was shown to be optimally active at 55 °C, pH 8.5 and alkali stable. A mutant, with higher transpeptidation activity, was obtained following two rounds of directed evolution using error-prone PCR and site-saturation mutagenesis. The mutation increased the ratio of transpeptidation to hydrolysis from 1.6 to 35.6. Additionally, Kinetic analysis exhibited 17.5% decrease of K m , 13.0-fold increase of K cat , and 16.3-fold increase of K cat / K m in mutant V319A/S437 G versus the wild-type. The 3-D modelling analysis revealed a tighter binding pocket in mutant V319A/S437 G. The frequency of hydrogen bond between donor substrate and two residues in the catalytic pocket (Gly437 and Thr375) was enhanced, which stabilized the ligand binding and thus improved the catalytic efficiency. The optimal conditions for the biocatalytic synthesis were determined as pH 10.0, 20 μg mL -1 Ba GT, 200 mM L-glutamine, 2 M ethylamine, and a reaction time of 5 h. The V319A/S437 G mutant was shown to increase the percentage yield of L-theanine from 58% to 83%. These results indicate the great potential of V319A/S437 G in L-theanine production after further study.
研究领域:
生物化学
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生物技术
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酶学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Yali Jiang,Yu Lu,Youyi Huang,Shouwen Chen,Zhixia Ji
Caffeine is a major component of tea and coffee. Apart from the stimulatory effects, cumulative intake of caffeine has caused several diseases. In addition, the presence of caffeine brings out the deterioration of environmental pollution. Therefore, the demand for decaffeinated products, especially decaffeinated coffee and tea, is increasing. However, Pseudomonas putida as the most widely studied bacteria isn't suitable for producing decaffeinated tea and coffee due to the production of an unpleasant odor and endotoxins at the same time. A more appropriate system ( Bacillus amyloliquefaciens HZ-12: ndmABCDE ) is needed to produce decaffeinated products; and HZ-12: ndmABCDE was constructed by orderly integrating N -demethylase genes ( ndmABCDE ) in a safer strain HZ-12. Demethylases (NdmABCDE) in P. putida can degrade caffeine to xanthine, which was further degraded to CO 2 and NH 4 + by the purine metabolic pathway. Finally, HZ-12: ndmABCDE could degrade 52.6% of caffeine in the optimized medium, 31.5% higher than HZ-12; caffeine was demethylated in sequence to form theobromine, paraxanthine, 7-methylxanthine and xanthine; and there are no endotoxins and unpleasant odor. This study provides a safer strain (HZ-12: ndmABCDE ) for producing decaffeinated tea and coffee and a new idea that B. amyloliquefaciens HZ-12 can heterologously express the demethylase genes in P. putida .
研究领域:
微生物学
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生物技术
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环境科学
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DOI
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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2017
·
IF 2.24
Jie Sun,Liang Chen,Bo Lou,Yanbing Bai,Xinjun Yu,Man Zhao,Zhao Wang
Sucralose is a non-nutritive artificial sweetener used in a broad range of foods and beverages. In the present study, Bacillus amyloliquefaciens WZS01 was isolated, identified, and used as a catalyst both in regioselective acylation and deacetylation for sucralose preparation. Bacterial cells were immobilized on polyurethane foam and utilized to synthesize sucrose-6-acetate regioselectively. The yield of sucrose-6-acetate was >95% with 60 mM sucrose after 22 h of reaction. Free cells could hydrolyze 75 mM sucralose-6-acetate to produce sucralose with >99% yield after 24 h of reaction. B. amyloliquefaciens WZS01 could be considered a potential biocatalyst for sucralose preparation.
研究领域:
微生物学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Jialin Wu,Guangqun Lan,Na He,Laping He,Cuiqin Li,Xiao Wang,Xuefeng Zeng
A producing-fibrinolytic enzyme strain was isolated with high yield. The strain was identified as Bacillus amyloliquefaciens . B. amyloliquefaciens GUTU06 fibrinolytic enzyme was purified by acetone precipitation and reverse micelle. Acetone precipitation condition and reverse micelle condition were examined. Results showed that the total reverse micelle extraction efficiency was 64.49 % ± 1.6 %. The purification fold of the entire process reached 13.38. The optimum pH of purified enzyme is 5, and the optimum temperature is 45 °C. Fe 3+ and K + can enhance the fibrinolytic activity of the enzyme. Compared to commercial fibrinolytic enzymes such as urokinase and lumbrukinase, GUTU06 fibrinolytic enzymes have a lower pH optimal range and higher temperature stability. The molecular weight of the enzyme was approximately 28 kDa. Reverse micelle extraction with cetyl trimethylammonium bromide as a surfactant combined with acetone precipitation is suitable for separating and purifying fibrinolytic enzymes and a promising technique for obtaining active proteins.
研究领域:
生物化学
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酶学
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微生物学
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生物技术
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 103
·期 13
·5367-5377
·
IF 3.67
Ding Lianshuai,Guo Wenbin,Chen Xinhua
The bacterium, Bacillus amyloliquefaciens Pc3, was previously isolated from Antarctic seawater and has been found to show antagonistic activity against the fungus, Rhizoctonia solani ACCC 36316, which causes a severe disease known as Sclerotinia sclerotiorum in rapeseed plants. Bacillus lipopeptides had been widely used as biocontrol agents for plant diseases. In this study, we isolated 11 lipopeptide compounds from B. amyloliquefaciens Pc3 culture broth via reversed-phase high-performance liquid chromatography (RP-HPLC) and used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to identify these as iturin A (C 14 , C 15 , C 16 , C 17 ), fengycin B (C 14 , C 15 , C 16 , C 17 ), and surfactin (C 14 , C 15 , C 16 ). We further found that the addition of exogenous alkanoic acids, including myristic acid, pentadecanoic acid, palmitic acid, heptadecanoic acid, octadecanoic acid, and nonadecanoic acid, to the bacterial growth media could promote lipopeptide production and enhance the antifungal activities of crude lipopeptide extracts from B. amyloliquefaciens Pc3. In addition, the transcriptional levels of three lipopeptide synthesis genes, ituD , fenA , and srfA-A , and two fatty acid metabolism–related genes, FabI , which encodes enoyl-ACP reductase, and FadB , which encodes enoyl-CoA hydratase, were found to be upregulated in cells grown with exogenous alkanoic acids. Among the six alkanoic acids tested, those with odd carbon chain lengths had a greater effect on lipopeptide production, antifungal activity, and target gene upregulation than those with even carbon chain lengths. These results provide a practical approach for the efficient production of lipopeptides in Bacillus amyloliquefaciens Pc3.
研究领域:
微生物学
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植物病理学
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生物技术
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DOI
高影响因子
危险品化学品经营许可证（带存储）