SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jintao Yan,Liangqiang Chen,Mengjing Teng,Mengdi Hao,Bin Feng,Fan Yang,Hao Shen,Shaoning Yu,Li Wang
Bacillus cereus is one of the most common foodborne pathogens and is widely distributed in foods, soil, water, plants and fodder. As an aerobic Gram-positive bacteria, B. cereus also plays an important role in liquor production, generating flavor enhancers and antibacterial substances. Therefore, a rapid and precise analysis strategy for this microbe is highly desired in food safety and sustainable food production. In this study, we propose an effective approach for B. cereus quantification using a dual recognition biosensor. B. cereus was specifically enriched with aptamer-functionalized magnetic beads, and then detected using the broad-spectrum fluorescent material, fragmented crystalline mannose-conjugated lectin-modified nano-MIL-53(Al)-NH 2 ( [email protected] ). The dual recognition method was applied for specific quantification of B. cereus in the range of 20–2 × 10 8 CFU/mL with a limit of detection of 4 CFU/mL within 60 min. Recoveries ranged from 93.8%−100.8% in milk, rice, chicken meat, eggs, and distilled yeast as real samples were also obtained.
研究领域:
微生物学
·
食品科学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Zhang Sinan,Wang Jihong,Wang Shuhua,Leng Su
Contamination of chlortetracycline (CTC) in water is a global problem that needs to be addressed urgently. Biochar immobilization of microorganisms is an effective way to remove CTC contamination from sewage. In this study, the CTC removal effect of free bacteria LZ01 and erding medicine residues biochar immobilized LZ01 was compared by shake flask batch experiments. The results showed that immobilized LZ01 significantly improved the CTC removal rate by 15.27% compared to free LZ01. The immobilization of LZ01 also significantly improved the environmental tolerance and the reusability and storage stability of strain LZ01. The optimal conditions for CTC removal (83.83%) by immobilized LZ01 were determined by response surface methodology: temperature of 33.98 °C, pH of 7.84, initial CTC concentration of 73.75 mg/L, and inoculation dose of 0.51% (w/v). The experimental results of treating CTC from simulated sewage showed that the immobilized LZ01 could remove more than 64% of CTC from the sewage. The ion of Cu 2+ accelerated the removal of CTC by immobilized LZ01, and the removal rates could reach 81.07%, 85.07%, and 87.50% in three sewages, i.e., river sewage, pond water, and farming wastewater, respectively. This study provides a feasible, effective, and rapid biological removal technique for the treatment of CTC pollution in sewage.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Hongyun Zhang,Ying Xia,Mingpeng Zhou,Jianyong Zheng,Zhao Wang,Yinjun Zhang
An intracellular esterase (BCE) from Bacillus cereus WZZ006 was purified to homogeneity with an 89.5-fold purification , specific activity of 1.79 U/mg, and 26.7% recovery. The estimated molecular weight of BCE was 96 kDa which was analyzed by SDS-PAGE and MALDI-TOF-MS. Activity staining denotes that BCE has an unexplored new carboxyl esterase characteristic. BCE enzyme activity was maximum at pH 8.5 and also at 50 °C with p NP-caproate as a substrate. This indicates that the studied BCE as a thermoalkaliphilic esterase. The kinetic properties like K m , V max , k cat and k cat/ K m value for BCE was found to be 0.98 mM, 0.03 mM/min, 69.47 min −1 and 70.89 mM −1 min −1 , respectively. Synthesis of ( S )-5-chloro-1-oxo-2,3-dihydro-2-hydroxy-1H-indole-2-carboxylic acid methyl ester (( S )-CODHCM) by BCE can be shortened to 3 h compared to 36 h with whole-cell catalysis. The e.e. s achieved was 93.83%, and conversion around 52.78% with E being 39.95. These features render BCE as a promising biocatalyst for the synthesis of a key chiral intermediate for indoxacarb.
研究领域:
生物化学
·
酶工程
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 104
·期 2
·765-774
·
IF 3.67
Tang Dandan,Liu Wei,Huang Lei,Cheng Leming,Xu Zhinan
25-hydroxyvitamin D 3 has attracted considerable attention due to its great medical value and huge market demand in animal husbandry. Microbial production of 25-hydroxyvitamin D 3 has been recognized as an alternative superior to traditional chemical synthesis. In this study, a Gram-positive bacteria zju 4-2 (CCTCC M 2019385) was isolated from the soil using vitamin D 3 as the sole carbon source and was identified as Bacillus cereus according to its physiological characteristics and 16S rRNA analysis, which also showed a relatively high capacity for 25-hydroxyvitamin D 3 production. Through systematic optimization of different catalytic conditions, the optimal solvent system of vitamin D 3 , vitamin D 3 addition time and concentration, temperature, and pH were shown to be propylene glycol/ethanol (v/v = 9:1), early stationary phase, 2 g/L, 37 °C, and pH 7.2, respectively. With these optimal conditions, 796 mg/L of 25-hydroxyvitamin D 3 was achieved after 48 h bioconversion with zju 4-2 at the shake flask level. Finally, up to 830 mg/L 25-hydroxyvitamin D 3 with a yield of 41.5% was obtained in a 5 L fermentation tank. Our developed biotransformation process with this newly isolated strain provides a platform to produce 25-hydroxyvitamin D 3 efficiently at industrialization scale.
研究领域:
微生物学
·
生物技术
·
医药科学
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xiaoting Song,Changzheng Shi,Huan Chen,Fanqiang Meng,Zhaoxin Lu,Xiaomei Bie
Detection of foodborne pathogens is of great significance for ensuring food safety and human health. Therefore, we developed a new detection method, which combined strand exchange isothermal amplification (SEA) with lateral flow assay strip (LFS), for the rapid and simple detection of Bacillus cereus in food. SEA technique is an isothermal nucleic acid amplification method based on the respiration of DNA denatured bubble to achieve a dynamic dissociation state of DNA double strand. The reaction requires only a pair of primers and DNA polymerase. The primers involved in the reaction were modified with biotin and 6-carboxyfluorescein (6-FAM) respectively, which could combine the reaction system with lateral flow assay strip to achieve the result of visualization of reaction results. In this reaction system, the detection sensitivity of B . cereus pure bacterial solution could reach 7.5×10 2 CFU/mL, and the specificity of the reaction system was good. Without enrichment treatment, the detection sensitivity of artificially contaminated rice cake samples and milk samples could reach 8.3×10 3 CFU/mL and 8.3×10 2 CFU/mL, respectively. The results of test strip and amplification were identical, which proved that the system was suitable for the detection of real samples. In addition, the established detection system has great potential as a preliminary detection tool in resource-limited areas.
研究领域:
微生物学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2025
·Vol. 78
·期 2
·e70015
·
IF 2.8
Yuxin Sun,Hongchao Dai,Wenwen Ge,Dan Xiong,Zhenquan Yang,Zhenbo Xu,Lei Yuan
Foodborne pathogen Bacillus cereus is widely distributed in dairy products and the processing environment. Beyond its threat to human health, the production of thermostable protease also poses a significant challenge to the dairy industry, particularly in pasteurised or ultra-high-temperature (UHT) processed milk products. Despite this, our understanding of dairy spoilage caused by B. cereus is very limited. This study aimed to evaluate the spoilage potential of a thermostable protease produced by B. cereus 12–1 in fluid milk. Specifically, crude protease characterisation, including optimum pH and temperature, the influence of Ca 2+ on enzyme activity and heat stability of protease, was studied. Moreover, the effects of this protease on the quality of both whole and skim UHT milk were evaluated, including zeta potential and particle size of milk, hydrolysis of milk protein, release of free amino acids and change of milk microstructure. This crude protease has relatively good stability at wide-ranging pH values from 5.0 to 10.0 (79.67–166.67 U/mL) and temperatures from 20°C to 70°C (92.67–165 U/mL), and maintained stability of 46.04% and 38.31% after heat treatment by 65°C/30 min or 100°C/10 min. Spoilage patterns in skim and whole UHT milk differ significantly due to variations in fat content. The protease caused protein hydrolysis in whole milk (15.25%) and skim milk (17.09%), effectively hydrolysed κ-casein and β-casein, caused the release of free amino acids (Lys, Val, Tyr, Phe, Ile, Met and Leu) in UHT milk and significantly increased particle size (increase by 940 nm for skim milk and 1319 nm for whole milk), and reduced zeta potential (reduction by 4 mV for skim milk and 3.83 mV for whole milk) of UHT milk. Results indicate the spoilage potential of crude protease from B. cereus and highlight the need to prevent dairy spoilage caused by B. cereus .
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 53
·34208-34216
·
IF 4.036
Yilan Zhang,Xiaoguang Ying,Bo Liu,Bo Yang,Xiao Li
Nanofiber membranes have recently been considered as promising supports for the immobilization of microorganisms due to the simplicity and cost-effectiveness of electrostatic spinning technology and the ability to control fiber morphology, such as obtaining higher surface area and porosity. In this study, electrospun polyvinyl alcohol/sodium alginate/attapulgite (PVA/SA/ATP) nanofiber membrane was prepared as support for immobilized Bacillus cereus LY-1 for diesel degradation in an aqueous medium and a significant improvement in diesel removal efficiency was realized. The effect of modified ATP concentration on diesel removal was investigated. The results showed that the nanofiber membranes complexed with cetyl trimethyl ammonium bromide (CTAB) and 1% ATP (w/w) had the best capacity for diesel removing. When the initial diesel concentration was 2 g L−1, about 87.8% of diesel was removed by the immobilized LY-1 cells after 72 h. Immobilization of bacteria improves the ability of bacteria to survive in adverse environments. Immobilized LY-1 cells maintain the nature to remove diesel at high salinity or pH range of 6–9. Furthermore, the reusability of the LY-1 cells-immobilized PVA/SA/CTAB–ATP nanofiber membrane was tested. A diesel removal rate of 64.9% could be achieved after 4 times of use. PVA/SA/CTAB–ATP nanofibrous membranes with immobilized LY-1 cells are feasible, economical and environmentally friendly for remediation of diesel contamination in the aqueous medium, and have potential applications in the future.
研究领域:
环境科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2023
·
IF 8.4
Guangzheng Jiang,Wenhui Xu,Xiaoxin Huang,Zhenqing Dai,Chunxia Zhou,Pengzhi Hong,Chengyong Li
The surface-enhanced Raman scattering (SERS) technique has demonstrated great advantages, such as high sensitivity and minimal equipment requirement. However, its application is largely hindered by high-expense SERS substrates. Here, we develop a low-cost and easily accessible SERS platform based on natural cuttlebone-derived organic matrix (CDOM) for rapid and portable detection of the Bacillus cereus spore biomarker, 2,6-pyridinedicarboxylic acid (DPA). The natural porous structure of CDOM helps achieve a significant load of Ag nanoparticles (AgNPs). By adjusting the amount of NaOH in Tollens’ reagent, more uniform AgNPs can be obtained on the surface of CDOM. The pyridine N atom or carboxylic acid O atom of DPA acts on the AgNP surface to produce the characteristic peak of SERS. Meanwhile, internal standard materials are added to resist the instability caused by natural materials. The SERS substrate realized the quantitative detection of DPA within 30 min. The linear range is from 40 to 1000 nM, and the detection limit is 8.62 nM. The sensor can detect bacterial spores in foods, such as milk. This method can be used as a reliable idea and an example for the practical application of biologically derived materials.
研究领域:
生物技术
·
食品安全检测
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2022
·
IF 5.911
Na Li,Xiaoming Yuan,Chun Li,Nuo Chen,Juan Wang,Bo Chen,Shubo Yu,Pengfei Yu,Junhui Zhang,Haiyan Zeng,Shi Wu,Xiaojuan Yang,Meiyan Yang,Jumei Zhang,Qingping Wu,Yu Ding
Bacillus cereus is a common foodborne pathogen that causes vomiting and diarrheal symptoms. Due to its spore-forming ability, B. cereus can resist physical sterilization and possess a relatively high contamination level in dairy products; therefore, it is necessary to develop an efficient strategy to control the growth of B. cereus . In this study, a novel bacteriophage, named DLn1, was isolated and characterized, and its endolysin was expressed. Morphological and genomic analyses revealed that the phage is a new species belonging to the Northropvirinae subfamily of the Salasmaviridae family. The life cycle and stability assays showed that the phage DLn1 exhibited a short latent period (15 min) and high burst size (618 plaque-forming units (PFU)/cell) and was tolerant to a wide range of pH (4–10) and temperature (4–55 °C) conditions. This lytic phage had narrow but specific host range to B. cereus strains, and could effectively reduce the number of B. cereus in milk within 6 h. More interestingly, the purified endolysin of phage DLn1 had a much wider lytic range and the inhibitory effect against B. cereus in milk was more efficient. Taken together, the new phage DLn1 and its endolysin could be promising biocontrol agents against B. cereus in dairy products.
研究领域:
微生物学
·
食品安全
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2023
·Vol. 195
·期 12
·7553-7567
·
IF 3
Li Xiaozheng,Jiang Junpo,Li Xinyue,Liu Dexu,Han Mengnan,Li Wei,Zhang Honglei
An efficient cofactor regeneration system has been developed to provide a hydride source for the preparation of optically pure alcohols by carbonyl reductase-catalyzed asymmetric reduction. This system employed a novel glucose dehydrogenase (BcGDH90) from Bacillus cereus HBL-AI. The gene encoding BcGDH90 was found through the genome-wide functional annotation. Homology-built model study revealed that BcGDH90 was a homo-tetramer, and each subunit was composed of βD-αE-αF-αG-βG motif, which was responsible for substrate binding and tetramer formation. The gene of BcGDH90 was cloned and expressed in Escherichia coli. The recombinant BcGDH90 exhibited maximum activity of 45.3 U/mg at pH 9.0 and 40 °C. BcGDH90 showed high stability in a wide pH range of 4.0–10.0 and was stable after the incubation at 55 °C for 5 h. BcGDH90 was not a metal ion-dependent enzyme, but Zn 2+ could seriously inhibit its activity. BcGDH90 displayed excellent tolerance to 90% of acetone, methanol, ethanol, n-propanol, and isopropanol. Furthermore, BcGDH90 was applied to regenerate NADPH for the asymmetric biosynthesis of ( S )-(+)-1-phenyl-1,2-ethanediol (( S )-PED) from hydroxyacetophenone (2-HAP) with high concentration, which increased the final efficiency by 59.4%. These results suggest that BcGDH90 is potentially useful for coenzyme regeneration in the biological reduction.
研究领域:
生物化学
·
酶工程
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROTECTION
·
2026
·Vol. 89
·期 11
·100927
·
IF 3.7
Qianhong Li,Jifeng Pu,Kanghui Li,Lingge Gao,Ruifen Wang,Yating Tang,Yanna Dai,Jing Zhao,Ting Zhou,Xin Wang,Hu Pan
Heat-resistant spore-forming bacteria are a primary cause of spoilage in ready-to-eat (RTE) foods, and effective control of spore contamination is critical for food preservation. Nisin, a GRAS antimicrobial peptide, exhibits potent activity against spore-forming bacteria, but its stage-specific effects during spore germination and outgrowth remain unclear. Heat-resistant Bacillus species were identified as the dominant spoilage bacteria in spoiled cooked beef, and B. cereus was selected to evaluate the inhibitory effects of nisin via phenotypic and transcriptomic assays. The minimum inhibitory concentration (MIC) of nisin against B. cereus spores was 400 µg/mL. Nisin slightly promoted spore germination, but significantly inhibited subsequent outgrowth of germinated spores. Time‑kill assays showed that 1 MIC nisin reduced viable counts to 3.09 log CFU/mL at 6 h compared with 8.05 log CFU/mL in controls; 2 MIC treatment for 2 h achieved a 0.65‑log reduction for intact spores but a 1.49‑log reduction for germinated spores. Nisin disrupted membrane permeability and integrity, triggered intracellular oxidative stress, leading to genomic DNA damage and reduced ATP synthesis. Transcriptomic analysis revealed 3049 differentially expressed genes, with disturbed energy metabolism and amino acid biosynthesis pathways. In cooked beef, nisin effectively inhibited B. cereus outgrowth and delayed product spoilage during storage. This study provides practical data support for nisin application to control spore-forming bacteria and extend the shelf life of RTE meat products.
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2024
·
IF 2.5
Hongchao Dai,Lei Yuan,Luyao Fan,Jia Yang,Xinan Jiao
This work assessed the prevalence and risk-related phenotypes of Bacillus cereus in milk products. Bacillus was the most dominant genus of 113 collected strains from 41 milk samples, and B. cereus (19.27%) was the most abundant species showing a high genetic diversity with seven random amplified polymorphic DNA patterns. Most B. cereus isolates were strong biofilm formers with good motility, extracellular polymeric substance-producing ability, high proteolytic and lipolytic activities. Antibiotic resistance analysis suggested the majority B. cereus strains were resistant to oxacillin (90.48%), tetracycline (95.24%), and penicillin G sodium (47.62%). Results revealed the genotypic diversity of B. cereus , and their potential risk in dairy industry.
研究领域:
微生物学
·
食品安全
·
乳品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）