PLoS One
·
2014
·Vol. 9
·期 6
·e100731
·
IF 3.534
Ke Xu,Ping Xu
Lactic acid is an important chemical with various industrial applications, and it can be efficiently produced by fermentation, in which Bacillus coagulans strains present excellent performance. Betaine can promote lactic acid fermentation as an effective osmoprotectant. Here, positive effect of betaine on fermentation by B. coagulans is revealed. Betaine could enhance lactic acid production by protecting l -LDH activity and cell growth from osmotic inhibition, especially under high glucose concentrations and with poor organic nitrogen nutrients. The fermentation with 0.05 g/L betaine could produce 17.9% more lactic acid compared to the fermentation without betaine. Beet molasses, which is rich in sucrose and betaine, was utilized in a co-feeding fermentation and raised the productivity by 22%. The efficient lactic acid fermentation by B. coagulans is thus developed by using betaine and beet molasses.
研究领域:
微生物学
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生物工程
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Peng Shudong,Chaoqun Guo,Songjie Wu,Hongchang Cui,Huayi Suo,Zhi Duan
The purpose of this paper was to evaluate the potential of plant-based drink based on soy protein and fructooligosaccharide in the development of probiotic beverages after fermentation by Bacillus coagulans VHProbi C08. B. coagulans VHProbi C08 was isolated from sour cabbage, and the sporulation rate was 90.03%. After being treated at 95 °C for 15 min, the survival rate was 6.86 log CFU/mL. The whole gene of VHProbi C08 contains only one chromosome, 4123 protein-coding genes are predicted, and 824 genes are unique. Plant-based drink fermented by VHProbi C08 showed the highest antibacterial activity against B. cereus ATCC 14579, followed by S. aureus ATCC 29213, L. monocytogenes ATCC 19115, S. enteritidis ATCC 14028, V. parahaemolyticus ATCC 17802, and E. coli ATCC 25922. Its DPPH radical scavenging activity, hydroxyl radical scavenging activity and anti-lipid peroxidation activity were 0.39, 0.25 and 0.35 mmol Trolox/L, respectively. After UPLC-MS/MS detection and analysis; 17 major metabolites with significant changes in fermented and unfermented plant-based drink were identified, including 2-Hydroxycinnamic acid, d -Glucarate, L-Tryptophan, Deoxycytidine, Imidazole propionic acid, Hypoxanthine, etc. In addition, the properties of plant-based drink fermented by VHProbi C08 was relatively stable during storage.
研究领域:
食品科学
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微生物学
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生物化学
✓ 已验证
DOI
高影响因子
Fermentation-Basel
·
2023
·Vol. 9
·期 9
·789
·
IF 3.7
William Rodrigues Alves,Thiago Alessandre da Silva,Arion Zandoná Filho,Luiz Pereira Ramos
This work aimed at producing lactic acid (LA) from sugarcane bagasse after steam explosion at 195 °C for 7.5 and 15 min. Enzymatic hydrolysis was carried out with Cellic CTec3 and Cellic HTec3 (Novozymes), whereas fermentation was performed withBacillus coagulansDSM2314. Water washing of pretreated solids before enzymatic hydrolysis improved both hydrolysis and fermentation yields. The presence of xylo-oligosaccharides (XOS) in substrate hydrolysates reduced hydrolysis efficiency, but their effect on fermentation was negligible. The presence of fermentation inhibitors in C5 streams was circumvented by adsorption on activated carbon powder with no detectable sugar losses. High carbohydrates-to-LA conversions (Yp/s) of 0.88 g·g−1were obtained from enzymatic hydrolysates of water-washed steam-exploded materials that were produced at 195 °C, in 7.5 min, and the use of centrifuged-but-never-washed pretreated solids decreased Yp/sby 16%. However, when the detoxified C5 stream was added at a 10% ratio, Yp/swas raised to 0.93 g·g−1for an LA productivity of 2.55 g·L−1·h−1. Doubling the pretreatment time caused a decrease in Yp/sto 0.78 g·g−1, but LA productivity was the highest (3.20 g·L−1·h−1). For pretreatment at 195 °C for 7.5 min, the elimination of water washing seemed feasible, but the use of longer pretreatment times made it mandatory to eliminate fermentation inhibitors.Keywords:sugarcane bagasse;steam explosion;enzymatic hydrolysis;fermentation;lactic acid
研究领域:
生物技术
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化学工程
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2020
·
IF 3.067
Lin Sui,Xuemei Zhu,Danyang Wu,Tian Ma,Yanfeng Tuo,Shujuan Jiang,Fang Qian,Guangqing Mu
Bacillus coagulans T242 has high lactase-producing activity and the lactase produced is thermally stable. However, the probiotic and other functional properties of the bacterium had not been studied and the bacterium needed further investigation for potential use in food products . This study aimed to evaluate the probiotic and functional characteristics of B. coagulans T242 in vitro . B. coagulans T242 has a high resistance to simulated gastric juice (pH 3.0) and bile salt (0.3%), indicating that it may have a good tolerance of gastrointestinal conditions. The adhesion of B. coagulans T242 to HT-29 cells was calculated as 58%. Additionally, B. coagulans T242 reduced the adhesion of Escherichia coli to HT-29 cells by up to 62%. B. coagulans T242 is sensitive to amoxicillin, amikacin , tetracycline, chloramphenicol, and rifampin, and is moderately sensitive to ciprofloxacin and erythromycin. These characteristics showed that B. coagulans T242 is a potential probiotic bacterium . B. coagulans T242 showed good cholesterol assimilation potential (19.9%) in vitro . In addition, B. coagulans T242 showed a high capacity for scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals (35.0%), hydroxyl radicals (39.0%), and superoxide anion radicals (14.8%), and good reducing power (58.5 μmol/L ascorbic acid equivalent). It was speculated that B. coagulans T242 might have the potential to exert antioxidant activity in vitro . These preliminary results suggested that B. coagulans T242 has the potential to be used as a probiotic.
研究领域:
微生物学
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食品科学
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营养学
✓ 已验证
DOI
高影响因子
Probiotics and Antimicrobial Proteins
·
2022
·Vol. 14
·期 4
·741-750
·
IF 5.265
Gao Xiaoxi,Zhang Yuhong,Mu Guangqing,Xu Yunpeng,Wang Xinmiao,Tuo Yanfeng,Qian Fang
The aim of the present study was to investigate the in vitro antioxidant potential of Bacillus coagulans T242. B. coagulans T242 showed better antioxidant activities, including the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging ability, lipid peroxidation inhibiting ability and reducing ability, than those exerted by Lactobacillus rhamnosus GG (LGG). B. coagulans T242 positively regulated the expression of the nuclear factor erythroid 2-relatedfactor 2/Kelch-like ECH-associated protein-1 (Nrf2/Keap1) pathway-related proteins (Nrf2, Keap1, heine oxygenase-1 (HO-1)); increased antioxidant enzymes (glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD)); reduced the content of malondialdehyde (MDA) level; decreased the expression of inflammatory-related cytokines interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α); and thus increased the survival rate in 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH)-damaged HT-29 cells. This study proved that B. coagulans T242 exerted antioxidative effects by quenching oxygen free radicals and activating the Nrf2 signaling pathway in HT-29 cells.
研究领域:
微生物学
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细胞生物学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
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2021
·
IF 3.352
Bing Zhang,Haoran Zhang,Yang Yu,Ruiqiang Zhang,Yanping Wu,Min Yue,Caimei Yang
ABSTRACT This study evaluated the effects of Bacillus coagulans ( B. coagulans ) as an alternative to antibiotics on growth performance, antioxidant capacity, immunity function and gut health in broilers. A total of 480 one-day-old broilers were randomly divided into 3 treatments with 8 replicates comprising 20 broilers each. The experiment lasted 42 d. Treatments included: basal diet without antibiotics (NCO); basal diet supplemented with 75 mg/kg chlortetracycline (ANT) ; basal diet supplemented with 5 × 10 9 CFU/kg B. coagulans (BC) . The B. coagulans enhanced body weight ( BW ) and average daily gain compared with the NCO group ( P < 0.05). However, there were no significant differences in average daily feed intake and feed: gain ratio ( F: G ) among three groups ( P > 0.05). The B. coagulans significantly increased catalase, superoxide dismutase, and glutathione peroxidase levels and reduced malondialdehyde levels ( P < 0.05). The serum immunoglobulins (IgA, IgM, and IgY) were significantly higher in the BC group when compared to the NCO and ANT groups ( P < 0.05). The B. coagulans also markedly reduced serum levels of proinflammatory factors (IL-1β, IL-6, and TNF-α) and enhanced anti-inflammatory factor (IL-10) concentrations compared with control group ( P < 0.05). Moreover, compared with the control group, BC significantly inhibited serum xanthine oxidase activity ( P < 0.05). The levels of acetic acid, propionic acid, butyrate, isobutyric acid and valerate in BC group were significantly increased on d 42 compared with the NCO and ANT groups ( P < 0.05). Furthermore, BC significantly altered cecal microbiota by reducing Desulfovibrio and Parasutterella , and by increasing Alistipes and Odoribacter ( P < 0.05, P < 0.05, P < 0.001, P < 0.01, respectively). In conclusion, dietary B. coagulans , when used as an alternative to antibiotics, improved body weight, average daily gain, antioxidant capacity, immunity function and gut health in broilers.
研究领域:
家禽科学
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微生物学
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免疫学
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营养学
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动物科学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2022
·
IF 6.796
Fang Ai,Xuedi Huang,Yalan Wu,Chen Ji,Yufang Gao,Ting Yu,Fujie Yan
Copper (Cu) is a kind of micronutrient element that is essential for human metabolism. However, it is also considered as an environmental pollutant which is toxic to organisms at a high concentration level. Probiotics , regarded as beneficial microorganisms for promoting human health, have functions of antioxidant capacity, immune-enhancing properties, intestinal barrier protection and regulation. Several studies have reported that probiotics show positive effects on alleviating and intervening heavy metals toxicity. However, evidence for relieving copper-induced toxicity by probiotics is still limited. In this study, we firstly conducted a zebrafish larvae model to screen out microorganisms which are helpful for CuSO 4 toxicity resistance and one novel strain named as Bacillus coagulans XY2 was discovered with the best protective activity. B. coagulans XY2 significantly reduced the mortality of zebrafish larvae exposed to 10 µmol/L CuSO 4 for 96 hr, as well as alleviated the neutrophils infiltration in the larvae lateral line under a 2 hr exposure. B. coagulans XY2 exhibited a high in vitro antioxidant activity and against CuSO 4 -induced oxidative stress in zebrafish larvae by up-regulating sod1, gstp1 and cat gene transcriptional levels and relevant enzymatic activities. CuSO 4 stimulated the inflammation process resulting in obvious increases of gene il-1β and il-10 transcription, which were suppressed by B. coagulans XY2 intervention. Overall, our results underline the bio-function of B. coagulans XY2 on protecting zebrafish larvae from copper toxicity, suggesting the potential application values of probiotics in copper toxicity alleviation on human and the environment.
研究领域:
环境毒理学
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微生物学
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水生生物学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2018
·
IF 5.807
Yujue Wang,Weifeng Cao,Jianquan Luo,Benkun Qi,Yinhua Wan
The aim of this study was to establish a simplified operational process for lactic acid (LA) production by Bacillus coagulans IPE22 from inedible starchy biomass with open fermentation method. First, 29.47 mU/mg specific amylase activity was detected in direct batch fermentation from soluble starch, but the activity of the produced amylase was too low for effective production of LA. Then seven batches from 72 g/L soluble starch were conducted without sterilization. It was found that one step simultaneous liquefaction, saccharification and fermentation (SLSF) with the addition of mesothermal α-amylase and glucoamylase was the optimal mode with LA concentration, yield and productivity of 68.72 g/L, 0.99 g/g and 1.72 g/L h respectively. Finally, inedible starchy biomass, cassava and sorghum flours, were proved to be alternatives to refined soluble starch. For the first time, one step open SLSF of inedible starchy biomass was reported for LA production by B. coagulans .
研究领域:
生物技术
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微生物学
·
发酵工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Clinical Nutrition ESPEN
·
2025
·
IF 2.6
Chunbo Zhang,Zequn Li,Shougen Cao,Xiaodong Liu,Yulong Tian,Jianfei Xu,Cheng Meng,Gan Liu,Hao Zhong,Peng Zhang,Yanbing Zhou
Background & Aims Intestinal barrier injury is widespread after treatment with antitumour drugs such as 5-fluorouracil (5-FU), but there is still a lack of prevention and effective management strategies based on fundamental research evidence. The aim of this study is to explore the effects and potential mechanisms of probiotic complexes on 5-fluorouracil (5-FU)-induced intestinal barrier damage in rats. METHODS An intestinal barrier injury model was established in male SD rats, aged 2 months, with a body weight ranging from 240 g to 260 g. via the intraperitoneal injection of 5-FU (70 mg/kg/d), and the probiotic complex (1 × 10 9 CFU/ml or 1 × 10 8 CFU/ml) was administered via gavage. The body weight, food intake and diarrhoea of the animals were monitored, euthanasia on the 9th day.Male Sprague-Dawley (SD) rats at 2 months of age and weighing 240 to 260 grams were selected. The animals were divided into an experimental group and a control group, with 6 rats in each group. A diarrhea and intestinal barrier dysfunction model was established by intraperitoneal injection of 5-fluorouracil. Specifically, the control group (n = 6) was intraperitoneally injected with normal saline (70 mg/kg), the 5-fluorouracil group (n = 6) was intraperitoneally injected with 5-fluorouracil (70 mg/kg, DBL®, Mayne Pharma Pty Ltd, Victoria, Australia), and the low-dose probiotic (PL) group (1×10 8 colony-forming units/mL, n = 6) and the high-dose probiotic (PH) group (1×10 9 colony-forming units/mL, n = 6) were intraperitoneally injected with 5-fluorouracil (70 mg/kg, DBL®, Mayne Pharma Pty Ltd, Victoria, Australia) from day 1 to day 5. The rats were gavaged once a day with a probiotic mixture suspended in 2 mL of phosphate-buffered saline (PBS) at a colony-forming unit (CFU) of 1×10 9 colony-forming units/mL/kg or 1×10 8 colony-forming units/mL/kg for 8 days. The rats in the control group and the 5-fluorouracil-treated group were gavaged with 2 mL of PBS daily from day 1 to day 8. The body weight, food intake, and diarrhea of the animals were monitored, and the rats were euthanized on day 9. Tissue samples from the colon and different small intestine segments were collected for histopathological examination. The levels of serum inflammatory factors and the expression levels of tight junction proteins and mucin-2 in ileum tissues were evaluated by hematoxylin-eosin staining, electron microscopy, enzyme-linked immunosorbent assay, Western blotting, real-time fluorescence quantitative reverse transcription polymerase chain reaction, and immunofluorescence. Fecal samples were collected for 16S rRNA gene sequencing and short-chain fatty acid detection. Results Compared with the control group, the probiotic complex intervention increased the food intake ( P < 0.05) of the experimental animals, delayed weight loss ( P < 0.05), alleviated diarrhoea ( P < 0.05), and improved the histopathological score of ileal mucosal injury ( P < 0.05). The probiotic complex intervention decreased the levels of IL-1β ( P < 0.05), IL-6 ( P < 0.001), MPO ( P < 0.001), TNF-α ( P < 0.001), and LPS ( P < 0.001) in the jejunum, whereas the level of the anti-inflammatory factor IL-10 increased ( P < 0.05). Additionally, MUC-2 and the mucosal barrier proteins occludin and claudin were upregulated at the gene expression level ( P < 0.05). In addition, the levels of acetic acid, propionic acid, and isobutyric acid in the colon increased, especially the concentration of butyric acid (P<0.01). It significantly reversed the decrease in Firmicutes and the simultaneous increase in Aspergillus ( P < 0.001) and reduced the enrichment of Akkermansiaceae bacteria ( P < 0.001). Furthermore, there was a significant increase in butyrate-producing bacteria, including Lactobacillus, acidophilus, Clostridium, UCG-014, Muridae , and Lachnospiraceae NK4A136 , in the intervention group. Conclusions Administration of probiotic complexes of Bacillus coagulans and Clostridium casei has the ability to improve intestinal mechanical, chemical, immune, and biological barrier functions and plays a protective role against 5-FU-induced intestinal barrier damage in rats. The potential mechanism needs further clarification.
研究领域:
药理学
·
胃肠病学
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肿瘤学
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微生物学
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免疫学
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营养科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2023
·
IF 3.5
Jianxin Zhang,Mengyuan Huang,Junchang Feng,Yongyan Chen,Meng Li,Xiaolin Meng,Xulu Chang
This study is aimed at exploring the effects of Bacillus coagulans NRS 609 on the growth performance, intestinal health, antioxidant activity, and immune response in common carp (Cyprinus carpio L.) by colonizing the intestinal tract with the beneficial strain. Gavage B. coagulans with GFP-tag at 109 CFU/mL into common carp for 7 days. Results indicated that B. coagulans were maintained in the intestine for at least 14 days. Four groups fed B. coagulans at different levels of inclusion, including 0 (CK), (A), (B), and (C) CFU/g of feed for 8 weeks. Experimental groups showed significantly better growth performance than the control (). The treatment groups had higher digestive enzyme activity, villus height, and muscle thickness than the control (). Supplementation with B. coagulans also altered the composition of the intestinal flora. In addition, an enhancement of the antioxidant enzyme and a reduction of malondialdehyde in the liver were observed with the addition of B. coagulans (). Moreover, the cytokines of IL-1β and IL-12, IL-10, and TGF-β, significant upregulation in experimental groups (). Based on the results, the optimal level of B. coagulans was 107 CFU/g in diets for common carp.
研究领域:
水产养殖
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微生物学
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免疫学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）