Journal of Cleaner Production
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2022
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IF 11.072
Yuan Chen,Yuancai Chen,Boxi Wang,Chenchen Mao,Chao Liu,Rongrong Liu,Bing Yan
Microbial reduction is a sustainable approach for both environmental remediation and recycling of precious metals from waste. Bacteria-mediated reduction of palladium ions (Pd 2+ ) in wastewater under mild conditions is such a strategy. However, external electron donors are usually required for palladium bioreduction. Herein, a novel gram-positive bacterium, Bacillus thuringiensis Y9 ( B. thuringiensis Y9), was found to reduce Pd 2+ to palladium nanoparticles (Pd-NPs) with endogenous electron donors. Transcriptomic analyses revealed 443 and 439 differentially expressed genes response to palladium treatment under anaerobic and aerobic conditions , respectively. It was found the genes encoding NADH-quinone oxidoreductase, dehydrogenases , cytochrome c reductase, cytochrome c oxidase , quinone cycle and ribE in B. thuringiensis Y9 have strong positive relationship to palladium reduction. The removal efficiencies of palladium by B. thuringiensis Y9 are as high as 93 mg g −1 under anaerobic conditions and 60 mg g −1 under aerobic conditions. Hydrogenase also plays vital role in the bioreduction process. The maximum Pd removal ratio of 99.18% was obtained with the primary influencing factors using response surface methodology (RSM). Our study provides a novel and sustainable strategy for Pd recovery from wastewater. The transcriptome data are valuable for understanding the global bioreduction mechanism of the microbial conversion of palladium.
研究领域:
环境科学
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微生物学
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材料科学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Long Wu,Xiaoyan Xiao,Kun Chen,Wenmin Yin,Qin Li,Pan Wang,Zhicheng Lu,Jing Ma,Heyou Han
Abstrcact Highly sensitive and selective detection of specific DNA sequences is of great importance in clinical diagnosis, environmental and food monitoring, but it still remains challenges to develop a facile method for real sample detection in aqueous solution. Here, a simple and recyclable surface enhanced Raman scattering (SERS) sensor was constructed for Bacillus thuringiensis (Bt) special gene fragment detection by Fe 3 O 4 magnetic beads (MBs) and Au–Ag core-shell nanorods (Au@Ag NRs). A hairpin DNA with sulfhydryl and biotin was attached to Au@Ag NRs as indicator, and MBs with streptavidin (SA) were acted as the capture probe. On the basis of the biotin–SA specific interaction, target sequences were first hybridized with the hairpin DNA and exposed the biotin. Subsequently, the Au@Ag NRs were captured by the streptavidin modified MBs, which reduced the suspended NRs and led to the change of Raman intensity . Under the optimal conditions, the SERS intensity revealed a good linearity with Bt transgene fragment ranging from 0.1 pM to 1 nM with a detection limit of 0.14 pM (S/N=3). To demonstrate the specificity of the strategy, the single-base mismatch in DNA was discussed in the SERS assay. The results showed that the sensitivity and accuracy of the proposed method was acceptable in DNA detection, revealing a great potential in special gene detection.
研究领域:
生物技术
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分析化学
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环境科学
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DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
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2015
·Vol. 26
·期 2
·451-457
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IF 1.927
Liu Cui,Zhou Zhen,Zou Linling,Cao Yuan-Cheng,Liu Jun’An,Lin Yongjun
A highly sensitive transgenic protein analysis method was proposed here based on fluorescein diacetate (FDA). First, FDA was prepared by the ball mill to harvest the nano-sized organic particles. Further examines showed that the FDA size can be controlled by the speed of centrifugation which can obtain FDA in well-distributed size. Cy3 antibody immobilization tests showed that the proteins can attach onto the FDA particles while keep bioactivities. FDA and Cry1Ac antibody immunoassay tests showed that when the FDA particle was in 150 nm, the linear range was 0.01 ng/L-30 μg/mL. And it has the lower detection limitation of 0.01 ng/L, which is 100 times more sensitive than the ELISA methods. These results indicate that the FDA related immunoassays are the promising approach in the transgenic analysis.
研究领域:
生物化学
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分子生物学
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食品科学
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DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
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2014
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IF 2.305
Huami Ming,Manli Wang,Hongzong Yin
Two novel surface plasmon resonance immunosensors were fabricated for detection of the Bacillus thuringiensis Cry1Ab protein and to demonstrate their performance in analyzing Cry1Ab protein in crop samples. Sensor 2 was modified by 1,6-hexanedithiol, Au/Ag alloy nanoparticles , 3-mercaptopropionic acid, and protein A (or not [sensor 1]), with Cry1Ab monoclonal antibody . As a result, both of the immunosensors exhibited satisfactory linear responses in the Cry1Ab protein concentration ranges of 10 to 500 ng ml −1 and 8 to 1000 ng ml −1 , and the detection limits were 5.0 and 4.8 ng ml −1 , respectively. The immunosensors possessed good specificity and acceptable reproducibility. In addition, crop samples could be analyzed after a simple treatment. The transgenic crops could be easily identified from the conventional ones by the two immunosensors.
研究领域:
生物技术
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分析化学
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农业科学
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DOI
PubMed
高影响因子
Advanced Science
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2024
·
IF 14.3
Meng Chen,Bingbing Guo,Hui Cheng,Weiyi Wang,Junyi Jin,Yingyi Zhang,Xiaolian Deng,Wenjun Yang,Chenyao Wu,Xiang Gao,Dehong Yu,Wei Feng,Yu Chen
Collaboration between cancer treatment and inflammation management has emerged as an integral facet of comprehensive cancer care. Nevertheless, the development of interventions concurrently targeting both inflammation and cancer has encountered significant challenges stemming from various external factors. Herein, a bioactive agent synthesized by genetically engineering melanin-producing Bacillus thuringiensis ( B. thuringiensis ) bacteria, simultaneously achieves eco-friendly photothermal agent and efficient reactive oxygen/nitrogen species (RONS) scavenger benefits, perfectly tackling present toughies from inflammation to cancer therapies. The biologically derived melanin exhibits exceptional photothermal-conversion performance, facilitating potent photonic hyperthermia that effectively eradicates tumor cells and tissues, thereby impeding tumor growth. Additionally, the RONS-scavenging properties of melanin produced by B. thuringiensis bacteria contribute to inflammation reduction, augmenting the efficacy of photothermal tumor repression. This study presents a representative paradigm of genetic engineering in B. thuringiensis bacteria to produce functional agents tailored for diverse biomedical applications, encompassing inflammation and cancer therapy.
研究领域:
生物技术
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基因工程
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癌症生物学
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炎症研究
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药学科学
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DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2022
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IF 8.91
Miaomiao Zhou,Jiyu Feng,Yuancai Chen,Yongyou Hu,Song Song
Doxycycline hydrochloride (DCH) could be continuously removed by Bacillus thuringiensis S622 with the in-situ biogenic manganese oxide (BioMnOx) via oxidizing/regenerating. The DCH removal rate was significantly increased by 3.01-fold/1.47-fold at high/low Mn loaded via the integration of biological (intracellular/extracellular electron transfer (IET/EET)) and abiotic process (BioMnOx, Mn(III) and •OH). BioMnOx accelerated IET via activating coenzyme Q to enhance electrons transfer (ET) from complex I to complex III, and as an alternative electron acceptor for respiration and provide another electron transfer transmission channel. Additionally, EET was also accelerated by stimulating to secrete flavins, cytochrome c (c-Cyt) and flavin bounded with c-Cyt (Flavins & Cyts). To our best knowledge, this is the first report about the role of BioMnOx on IET/EET during antibiotic biodegradation. These results suggested that Bacillus thuringiensis S622 incorporated with BioMnOx could adopt an alternative strategy to enhance DCH degradation, which may be of biogeochemical and technological significance.
研究领域:
微生物学
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环境科学
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
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2022
·
IF 6.796
Shunzhang Chen,Junyuan Gong,Yanxia Cheng,Yuqi Guo,Feize Li,Tu Lan,Yuanyou Yang,Jijun Yang,Ning Liu,Jiali Liao
For a broader understanding of uranium migration affected by microorganisms in natural anaerobic environment, the bioreduction of uranium(Ⅵ) (U(Ⅵ)) was revealed in Bacillus thuringiensis , a dominant bacterium strain with potential of uranium-tolerant isolated from uranium contaminated soil. The reduction behavior was systematically investigated by the quantitative analysis of U(Ⅳ) in bacteria, and mechanism was inferred from the pathway of electron transmission. Under anaerobic conditions, appropriate biomass and sodium lactate as electron donor, reduction behavior of U(Ⅵ) induced by B. thuringiensis was restricted by the activity of lactate dehydrogenase, which was directly affected by the initial pH, temperature and initial U(Ⅵ) concentration of bioreduction system. Bioreduction of U(Ⅵ) was driven by the generation of nicotinamide adenine dinucleotide (NADH) from enzymatic reaction of sodium lactate with various dehydrogenase. The transmission of the electrons from bacteria to U(Ⅵ) was mainly supported by the intracellular NADH dehydrogenase-ubiquinone system, this process could maintain the biological activity of cells.
研究领域:
环境微生物学
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生物地球化学
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放射化学
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DOI
PubMed
高影响因子
Biocatalysis and Agricultural Biotechnology
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2019
·
IF 0
Feng-Qin Gong,Qi-Shun Liu,Hai-Dong Tan,Tang Li,Cheng-Yu Tan,Heng Yin
Acetoin (3-hydroxy-2-butanone) is an important four-carbon compound widely used in the food industry and other industrial applications. This study aimed to identify a novel butanediol dehydrogenase that can efficiently catalyze the formation of acetoin. A novel butanediol dehydrogenase, Bt BDH, was identified from Bacillus thuringiensis subsp. Kurstaki ACCC 10066, and its enzymatic properties were characterized. The optimum pH and temperature for the oxidation activity of Bt BDH were 10.0 and 50 °C, respectively, and those for the reduction activities were 7.5 and 35 °C, respectively. In addition, it exhibits stability over a wide pH range (6–10) and temperatures up to 70 °C. Bt BDH showed good stability after storage for 3 months at 4 °C. Moreover, ethylenediaminetetracetic acid (EDTA) inhibits the enzymatic activity of Bt BDH, indicating that the enzyme is metal-dependent. This study characterized a novel (2R,3R) −2,3-butanediol dehydrogenase. Its excellent oxidation activity and stability ensure its great industrial application potential in the production of acetoin.
研究领域:
生物化学
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微生物学
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酶学
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DOI
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Jiyuan Liang,Yuxiang Wu,Cui Liu,Yuan-Cheng Cao,Jun’An Liu,Yongjun Lin
The silicon coated Magnetic nanoparticles (MNps) were prepared and applied to the genetically modified detection for Cry1Ac protein. TEM observations showed the bare MNps were in the size of 3–5 nm and the silicon coated core/shell MNps were in the range of 30–60 nm 1 M HCl acidic solution was used to test the chemical stability of the resultant MNps and results showed the coated MNps were very stable in the acid environment even after 12 h. Cy3 labelled IgG tests showed that the probe on the MNps was kept the bioactivity in the immunoassays. MNps based detection results showed that this analytical strategy had similar linear relation which R = 0.995 with the commercial ELISA kit. The MNps based detections were reliable for the Cry1 Ac protein in the linear range from 0.1 pg/L to 1.0 mg/L, and showed better performance in the low concentration of analyte. This study provides a promising technique to detect the Cry1Ac protein with high sensitivity in the examination of the GM products which is of importance in food safety.
研究领域:
生物技术
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食品安全
·
纳米科技
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2022
·
IF 10.753
Limei Cai,Xiaoming Liu,Zhiqiang Tian,J.P. Michaud,Zhongjian Shen,Zhen Li,Songdou Zhang,Xiaoxia Liu
The widespread adoption of Bt crops expressing insecticidal proteins derived from Bacillus thuringiensis has created a need to assess the potential effects of these toxins on non-target organisms, especially species such as Arma custos , a generalist predator that provides important biological control services in many field crops in Asia. Direct dietary exposure of A. custos to Cry1Ah and Vip3Aa proteins produced no adverse effects on life history traits, despite continuous exposure throughout development and early adult life to concentrations significantly higher than the Bt protein concentration likely encountered by A.custos in the field, even when feeding directly on Bt plants. Enzyme-linked immunosorbent assay confirmed the presence of Bt proteins in A. custos midguts, but quantitative real-time PCR analysis of 12 genes associated with detoxification, antioxidative responses, immune responses, and metabolism revealed no significant changes in expression in adult bugs. Indirect exposure to these toxins via consumption of intoxicated prey, larvae of Helicoverpa armigera (Hübner), likewise produced no negative impacts on survival, development, adult weight, or female fecundity in either the F0 (exposed) or F1 (unexposed) generation, but female fresh weight was reduced in the F0 generation by the Cry1Ah (50 μg/g) treatment. Finally, a competitive binding assay with labelled protein and a ligand blotting assay both demonstrated that the Cry1Ah protein could not bind to receptors on the midgut brush border membrane vesicles (BBMVs) of A. custos adults. Therefore, we conclude that Cry1Ah and Vip3Aa proteins are unlikely to have significant negative effects on A. custos populations if employed as plant-incorporated protectants in field crops.
研究领域:
昆虫学
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环境科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
BIOCONTROL SCIENCE AND TECHNOLOGY
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2017
·
IF 0.919
Xiaohua Hu,Yajie Guo,Songqing Wu,Zhaoxia Liu,Tao Fu,Ensi Shao,Carballar-Lejarazú Rebeca,Guohui Zhao,Zhipeng Huang,Ivan Gelbič,Xiong Guan,Shuangquan Zou,Lei Xu,Lingling Zhang
Bacillus thuringiensis var. israelensis (Bti) is highly pathogenic to mosquito larvae and is widely used for mosquito control. Its mosquitocidal activity however is relatively low compared to many chemical insecticides. The detoxification mechanisms in the mosquito, among other things, might neutralize the Bti activity, resulting in resistance or tolerance. We tested whether or not the detoxification mechanisms against chemical insecticides might also operate against Bti, rendering it less effective. We targeted four enzymes in Aedes aegypti larvae involved in detoxification with inhibitors that have been used in resistance studies in chemical insecticides and assayed their effects on Bti toxicity. Results revealed that phenylmethanesulphonyl fluoride (PMSF), diethyl maleate, phenobarbital (PB), and piperonyl butoxide (PBO) altered Bti toxicity to various degrees. PMSF is a serine protease inhibitor that prevents Bti digestion and improves Bti activity. PB that induces several detoxifying enzymes had two different effects depending on the method of treatment. Mortality was higher when treatment with PB was discontinuous (149%) whereas with continuous treatment it was lower (101%). PBO, a typical cytochrome P450 inhibitor, increased Bti effect (159%). The combination of discontinuous pretreatment of larvae with PB followed by PBO had a synergistic effect and showed increased activity (146%). It appears that the mechanism for Bti resistance in mosquitoes is similar to that of chemical insecticides. Our studies indicate that we may be able to increase Bti activity by inhibiting some of the detoxification systems as active as broad spectrum chemical insecticides.
研究领域:
昆虫学
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毒理学
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杀虫剂抗性
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蚊虫控制
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产品关联
DOI
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2022
·
IF 3.705
Kangli Wu,Meiling Shi,Xiaomei Pan,Jianqiang Zhang,Xiaomeng Zhang,Tong Shen,Yongqiang Tian
In this study, a ligninolytic enzyme-producing strain F5 was isolated and identified as Bacillus thuringiensis , which can efficiently degrade methylene blue (MB) dye. The optimal pH, temperature, rotation speed, NaCl concentration, and inoculum of strain F5 for MB degradation were pH 6.0, 30 °C, 140 rpm, 10 g/L NaCl, 4% inoculum (v/v), and the strain F5 had salt tolerance, the MB decolorization rate reached 95% after 12 h. The degraded products were characterized by UV–vis, FT-IR, and GC-MS. Based on products analysis, four different intermediates were identified, and a new pathway for the degradation of MB was proposed. The degradation of MB by strain F5 was due to the synergistic effects of laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP), and NADH-DCIP reductase ; among them, Lac and MnP were the key enzymes. The phytotoxicity results showed that MB degraded metabolites' toxicity was lower than that of the parent compound, indicating that the strain F5 had a detoxification effect on MB dyes.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
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