INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Song Li,Xiaomao Peng,Zengbo Wang,Chunhua Chen,Xin Li,Qixing Nie,Xiaojun Huang,Shuigen Bian,Junyi Yin,Steve W. Cui,Huizi Tan,Shaoping Nie
Pectins, a complex class of polysaccharides, are prominently represented by HG-chains, which are both abundant and extensively studied. Bacteroides species exhibit a remarkable ability to metabolize plant- and animal-derived polysaccharides, including the degradation of HG-type pectins through polysaccharide utilization locus (PUL). However, the effects of structurally diverse HG-type pectins on PUL expression, metabolite production, and metabolic pathways of Bacteroides remain unclear. Addressing this, this study chose HG-type pectins with different molecular weights from citrus (CP) and pomelo (PP), identified Bacteroides species capable of utilizing these pectins, studied the resulting metabolites through non-targeted metabolomics coupled with short-chain fatty acids analysis, and examined the activation of PUL and metabolic pathways by transcriptomic studies. The results showed that Bacteroides thetaiotaomicron A4 and Bacteroides caccae K9 could utilize HG-type pectins with different molecular weights. The production of propionic acid by Bacteroides thetaiotaomicron A4 was significantly affected by the molecular weight of pectins. Utilizing CP, B. thetaiotaomicron A4 enriched metabolites such as carbohydrates, amino acids, peptides, amines, and significantly enhanced pathways such as sphingolipid metabolism and drug metabolism (other enzymes) through PUL75, PUL63, and PUL55. In contrast, when using PP, B. thetaiotaomicron A4 enriched similar metabolites and further upregulated pathways related to sphingolipid metabolism and pyrimidine metabolism. The molecular weight of HG-type pectins differentially affected the expression of carbohydrate-active enzymes and metabolic pathways, resulting in different metabolite profiles. This study aims to contribute to the understanding of structure-activity relationship between pectins and gut microbiota and to inform precision nutrition strategies.
研究领域:
微生物学
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营养学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2024
·
IF 4
Shuo Xie,Junze Ma,Zheng Lu
This study probes into the unique metabolic responses of Bacteroides thetaiotaomicron (B. thetaiotaomicron), a key player in the gut microbiota, when it metabolizes rhamnose rather than typical carbohydrates. Known for its predominant role in the Bacteroidetes phylum, B. thetaiotaomicron efficiently breaks down poly- and mono-saccharides into beneficial short-chain fatty acids (SCFAs), crucial for both host health and microbial ecology balance. Our research focused on how this bacterium’s SCFA production differ when utilizing various monosaccharides, with an emphasis on the oxidative stress responses triggered by rhamnose consumption. Notably, rhamnose use results in unique metabolic byproducts, including substantial quantities of 1,2-propanediol, which differs significantly from those produced during glucose metabolism. Our research reveals that rhamnose consumption is associated with a reduction in reactive oxygen species (ROS), signifying improved resistance to oxidative stress compared to other sugars. This effect is attributed to specific gene expressions within the rhamnose metabolic pathway. Notably, overexpression of the rhamnose metabolism regulator RhaR in B. thetaiotaomicron enhances its survival in oxygen-rich conditions by reducing hydrogen peroxide production. This reduction is linked to decreased expression of pyruvate:ferredoxin oxidoreductase (PFOR). In contrast, experiments with a rhaR-deficient strain demonstrated that the absence of RhaR causes B. thetaiotaomicron cells growing on rhamnose to produce ROS at rates comparable to cells grown on glucose, therefore, losing their advantage in oxidative resistance. Concurrently, the expression of PFOR is no longer suppressed. These results indicate that when B. thetaiotaomicron is cultured in a rhamnose-based medium, RhaR can restrain the expression of PFOR. Although PFOR is not a primary contributor to intracellular ROS production, its sufficient inhibition does reduce ROS levels to certain extent, consequently improving the bacterium’s resistance to oxidative stress. It highlights the metabolic flexibility and robustness of microbes in handling diverse metabolic challenges and oxidative stress in gut niches through the consumption of alternative carbohydrates.
研究领域:
微生物学
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代谢组学
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肠道健康
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DOI
PubMed
高影响因子
Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Zhao Yi,Dai Zhengqun,Lang Yanmei,Li Rui,Zheng Hongyu,Mi Jiajia,He Xiu,Liu Jin,Xiang Rong,Mei Xueran,Liu Yang,Wang Yantang,Guo Huijie,Yang Qian,Ren Ke,Yang Tai
Chemotherapy-induced diarrhea (CID) is a frequent gastrointestinal side effect in cancer patients, particularly associated with the use of 5-fluorouracil (5-FU). This study aimed to isolate multiple Bacteroides strains from the feces of healthy individuals and identify Bacteroides eggerthii ( B. eggerthii ) S13-F8 as the optimal candidate for alleviating CID. Whole-genome sequencing of B. eggerthii S13-F8 was conducted to uncover its functional characteristics and explore the potential mechanisms underlying its protective effects against CID. The anti-CID efficacy of B. eggerthii S13-F8 was assessed using multiple parameters, including diarrhea severity, food intake, and body weight changes. Comprehensive analyses, including blood tests, intestinal histopathology, colon transcriptomics, and fecal metagenomics, were performed to elucidate its underlying mechanisms. In a 5-FU-induced mouse model, B. eggerthii S13-F8 significantly alleviated weight loss and diarrhea. Histological examination revealed that B. eggerthii S13-F8 preserved the villus height-to-crypt depth (V/C) ratio and protected goblet cells in colonic tissues. Gene expression analysis showed that B. eggerthii S13-F8 upregulated protective markers, such as Aqp8 , Slc26a3 , and mucin-related genes ( TFF3 , FCGBP , and Muc2 ), while downregulating pro-inflammatory mediators, including IL-1α , IL-22 , and Cxcl2 . Furthermore, B. eggerthii S13-F8 modulated gut microbiota composition by suppressing pathogenic bacteria ( Pseudomonas aeruginosa , Salmonella , γ-Proteobacteria, and Shigella ) and enriching beneficial taxa, such as Lactobacillus and Akkermansia muciniphila . In conclusion, B. eggerthii S13-F8 demonstrates significant potential in mitigating severe diarrhea caused by 5-FU chemotherapy, providing a strong foundation for its development as a live biotherapeutic for CID treatment.
研究领域:
医学
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微生物学
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基因组学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
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IF 8.2
Xiaoxiang Gao,FuSheng Xu,Tangjun Li,Pan Huang,Leilei Yu,Fengwei Tian,Jianxin Zhao,Wei Chen,Qixiao Zhai
Improving Bacteroides cellulosilyticus abundance is a feasible approach to treating inflammatory bowel disease (IBD). Although B. cellulosilyticus is responsive to dietary components, untargeted manipulation cannot focus on target microbe and lead to an increase in harmful bacteria in the microbiota . Breakthroughs in methods for regulating specific microbes, but the protocols are expensive, time-consuming, and difficult to follow. Glycans based on microbial-carbohydrate-active enzymes (CAZymes) would provide a potential solution. We propose a method based on CAZymes to explore polysaccharides that target specific gut microbes and alleviate diseases. The designed polysaccharides (Arabinogalactan, AG) enrich the abundance of B. cellulosilyticus in single-strain co-cultures, fermentation in vitro , and mouse models in vivo . Supplementation with AG relieved mice from colitis and clinical symptoms. We reveal that AG directly alters B. cellulosilyticus level and cooperative microbes, resulting in remission of colitis. Our glycan design pipeline is a promising way to improve disease through the targeted enhancement of specific microbes.
研究领域:
微生物学
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肠道健康
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分子生物学
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DOI
高影响因子
Food Frontiers
·
2021
·Vol. 2
·期 2
·218-231
·
IF 0
Song Li,Baojie Zhang,Jielun Hu,Yadong Zhong,Yonggan Sun,Shaoping Nie
The interaction between gut microbiota and polysaccharide is being paid more and more attention. Galactan is a kind of polysaccharide mainly composed of galactose, and it has been shown to play a significant role in regulating gut microbiota. Bacteroides thetaiotaomicron is considered as the best degrader of polysaccharides. The purpose of this paper was to investigate the utilization of four different galactans, including agarose, carrageenan, arabinogalactan, and glucofucogalactan, by our own isolated B. thetaiotaomicron A4. Samples of cultures grown on either four galactans groups or control groups were collected. The change of OD 600 , pH, and short-chain fatty acids (SCFAs) during fermentation were determined, and growth curve and transcriptome of B. thetaiotaomicron A4 were studied. B. thetaiotaomicron A4 could utilize the four galactans and grew well on them, with carrageenan being most utilized, followed by arabinogalactan, glucofucogalactan, and agarose. SCFAs (mainly acetic acid and propionic acid) produced along with the decreased pH during fermentation. A large number of genes of B. thetaiotaomicron A4 were upregulated and functioned in different pathways during the degradation of the four galactans. The carbohydrate metabolism-related pathways of B. thetaiotaomicron A4 were enriched after feeding the four galactans, although the specific pathways were different among four galactans groups. The different structural characteristics of four galactans required that B. thetaiotaomicron A4 could excrete corresponding enzymes to degrade them. These results help to understand the interaction between galactans and gut microbe.
研究领域:
微生物学
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分子生物学
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肠道微生物
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DOI
JOURNAL OF MEDICAL VIROLOGY
·
2025
·Vol. 97
·期 5
·e70395
·
IF 4.6
Qiuhong You,Kaifeng Wang,Zhou Zhao,Heqi Zhou,Zhixian Lan,Hongyan Liang,Rui Deng,Wanying Li,Sheng Shen,Routing Wang,Kaikai Zhang,Dekai Zheng,Jian Sun
In chronic hepatitis B (CHB) patients under antiviral treatment, liver injury, as evidenced by elevated alanine transaminase (ALT), is associated with unfavorable outcomes and needs effective treatment. The interaction between gut microbiota and liver injury in CHB patients remains unclear. Using a case-control design, 28 cases with elevated ALT and 28 matched controls with normal ALT were randomly selected from CHB patients with viral control. Clinical characteristics were comparable between groups. Metagenomic sequencing revealed that Bacteroides fragilis was decreased in cases and exhibited the greatest disparity between cases and controls. Mice colonized by gut microbiota from cases exhibited more severe liver damage in both LPS-induced and MCD diet-induced liver injury models, and had a lower abundance of B. fragilis compared to mice colonized by gut microbiota from controls. Oral gavage of B. fragilis improved both LPS-induced and MCD diet-induced liver injury. Metabolomics analysis revealed that the levels of 7-Ketolithocholic acid (7-Keto-LCA) were positively correlated with B. fragilis and significantly increased in the cultural supernatant of B. fragilis . Consistently, 7-Keto-LCA exerted protective effects against both LPS-induced and MCD diet-induced liver damage. Targeting gut microbiota might be a promising therapeutic treatment for alleviation residual liver inflammation in CHB patients with viral control.
研究领域:
医学
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微生物学
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代谢组学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jinghong Wei,Wangting Zhou,Wei Yi,Jiameng Shi,Yamei Yan,Jia Mi,Lu Lu,Youlong Cao,Xiaoxiong Zeng
Polysaccharides from the fruits of Lycium barbarum L. (LBPs) are the principal active constituents of L. barbarum , and it has been reported that LBPs exert regulatory effects on the gut microbiota, particularly in promoting the growth of Bacteroides . In this study, the in vitro anaerobic fermentation revealed that LBPs-4, a purified fraction of the crude LBPs, increased the relative abundances of 11 Bacteroides species, particularly B. ovatus . Results of the pure cultures indicated B. ovatus , B. thetaiotaomicron , B. fragilis and B. uniformis could effectively utilize LBPs-4 for their own growth. Transcriptome analysis of B. ovatus indicated that the degradation of LBPs-4 involved the up-regulation of three polysaccharide utilization loci (PULs). Among them, Bovatus _00022 in PUL1, which belongs to the GH28 family and is annotated as a polygalacturonase, is one of the major carbohydrate-active enzymes in the metabolism of LBPs-4. BO PG00022 was preliminarily confirmed to be effective in the degradation via the hydrolysis analysis. In conclusion, this study provided significant insights into the degradation mechanism of LBPs-4 employing gut microflora or enzymes as targets, providing a theoretical basis for the precise utilization and structural verification of complex glycans.
研究领域:
微生物学
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分子生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
Cell Host & Microbe
·
2024
·
IF 20.6
Haiyuan Shen,Liangliang Zhou,Hao Zhang,Yuanru Yang,Ling Jiang,Dongqing Wu,Hang Shu,Hejiao Zhang,Linxi Xie,Kaichen Zhou,Chen Cheng,Lei Yang,Jiali Jiang,Siya Wang,Yiran Han,Jiayi Zhu,Long Xu,Zhihua Liu,Hua Wang,Shi Yin
The gut microbiota and diet-induced changes in microbiome composition have been linked to various liver diseases, although the specific microbes and mechanisms remain understudied. Alcohol-related liver disease (ALD) is one such disease with limited therapeutic options due to its complex pathogenesis. We demonstrate that a diet rich in soluble dietary fiber increases the abundance of Bacteroides acidifaciens (B. acidifaciens) and alleviates alcohol-induced liver injury in mice. B. acidifaciens treatment alone ameliorates liver injury through a bile salt hydrolase that generates unconjugated bile acids to activate intestinal farnesoid X receptor (FXR) and its downstream target, fibroblast growth factor-15 (FGF15). FGF15 promotes hepatocyte expression of ornithine aminotransferase (OAT), which facilitates the metabolism of accumulated ornithine in the liver into glutamate, thereby providing sufficient glutamate for ammonia detoxification via the glutamine synthesis pathway. Collectively, these findings uncover a potential therapeutic strategy for ALD involving dietary fiber supplementation and B. acidifaciens.
研究领域:
胃肠病学
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肝病学
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微生物学
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营养学
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DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 10
·期 7
·4304-4314
·
IF 3.241
Chunqing Ai,Pingrui Jiang,Yili Liu,Mengmeng Duan,Xiaona Sun,Tengrui Luo,Guoping Jiang,Shuang Song
Accumulating studies showed that the beneficial actions of polysaccharides were closely associated with an improvement of the gut microbiota, but mechanisms that link polysaccharides and gut microbiota alterations are ill defined. Alginate from Laminaria japonica (LJP-A) can avoid being digested by the upper digestive tract and reach the colon where it can improve the gut microbiota community. LJP-A increased the relative abundance of some Bacteroidaceae members, e.g. Bacteroides species. The distribution of Bacteroides species in the gut is specific to the individual, and the specific use of LJP-A by Bacteroides species can determine LJP-A-induced alterations of the intestinal Bacteroides community, in which carbon source-based syntrophic interactions occur in Bacteroides species of the human gut microbial ecosystem. Compared to other species, Bacteroides finegoldii responded more positively to LJP-A intervention, and its properties may have a close connection with the bioactivities of LJP-A. This study provides a rationale for personalized treatment with dietary polysaccharides that were optimally fermented within a particular individual.
研究领域:
微生物学
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营养学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Gut Microbes
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2025
·
IF 11
Xinwei Li,Xia Xiao,Shengnan Wang,Biyu Wu,Yixuan Zhou,Pan Deng
Polyamines are important gut microbial metabolites known to affect host physiology, yet the mechanisms behind their microbial production remain incompletely understood. In this study, we developed a stable isotope-resolved metabolomic (SIRM) approach to track polyamine biosynthesis in the gut microbiome. Viable microbial cells were extracted from fresh human and mouse feces and incubated anaerobically with [U-13C]-labeled inulin (tracer). Liquid chromatography-high resolution mass spectrometry analysis revealed distinct 13C enrichment profiles for spermidine (SPD) and putrescine (PUT), indicating that the arginine-agmatine-SPD pathway contributes to SPD biosynthesis in addition to the well-known spermidine synthase pathway (PUT aminopropylation). Species differences were observed in the 13C enrichments of polyamines and related metabolites between the human and mouse microbiome. By analyzing the fecal metabolomics and metatranscriptomic data from an inflammatory bowel disease (IBD) cohort, we found significantly higher polyamine levels in IBD patients compared to healthy controls. Further investigations using single-strain SIRM and in silico analyses identified Bacteroides spp. as key contributors to polyamine biosynthesis, harboring essential genes for this process and potentially driving the upregulation of polyamines in IBD. Taken together, this study expands our understanding of polyamine biosynthesis in the gut microbiome and will facilitate the development of precision therapies to target polyamine-associated diseases.
研究领域:
微生物学
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代谢组学
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医学
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产品关联
DOI
PubMed
高影响因子
Gut Microbes
·
2025
·
IF 11
Tong Jin,Si-Yuan Li,Hong-Li Zheng,Xiao-Dan Liu,Yue Huang,Gan Ma,Ya-Xuan Zhao,Xiao-Tian Zhao,Li Yang,Qi-Hui Wang,Hong-Jun Wang,Chengyong Gu,Zhiqiang Pan,Fuqing Lin
The gut microbiota has been linked to a number of neurological disorders. However, it is unclear whether the gut microbiota is involved in the genesis of chronic itch, a refractory condition that afflicts patients both physically and mentally. Here, we report that depletion of gut microbiota enhances tolerance to itch in mice orally administered with antibiotics (ABX) and mice free of germ. Of note, oral gavage with Bacteroides fragilis (B. fragilis), a prominent species of the genus Bacteroides with most differential change, corrected the ABX-induced itch dysfunction through its driven metabolite acetyl-l-carnitine (ALC). Mechanistically, gut microbiota or B. fragilis depletion caused a decrease in RNA N6-methyladenosine (m6A) demethylase FTO expression in the dorsal horn and a consequent increase in RNA m6A sites in Mas-related G protein-coupled receptor F (MrgprF) mRNA, leading to decreased MRGPRF protein. The downregulation of FTO was triggered by inactivation of ETS proto-oncogene 1 (ETS1), a transcription factor that binds to the Fto promoter. These findings support a gut microbe – spinal connection in modulation of itch sensation in RNA m6A epigenetic-dependent manner and highlight a critical role of ALC in linking the altered B. fragilis and itch dysfunction.
研究领域:
神经科学
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微生物学
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表观遗传学
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产品关联
DOI
PubMed
高影响因子
NEURON
·
2026
·
IF 15
Yicong Jia,Hong Chen,Qianhui Zou,Sijing Chen,Jiahao Li,Yiming Chen,Liming Lu,Feng Hong,Shuhui Jia,Xiaoyuan Jing,Jiayan Ren,Fahim Muhammad,JiaYu Mi,Jing Duan,Jianxiang Liao,Qing Liu,Fuqiang Xu,Paul J. Kenny,Ming-Hu Han,Liping Wang,Zuxin Chen,Dezhi Cao,Xin-an Liu
Gut dysbiosis has been implicated in epilepsy, yet probiotic efficacy and mechanisms remain unclear. Here, we identify that Bacteroides fragilis ( B. fragilis ) is markedly reduced in children with epilepsy and show that oral B. fragilis administration suppresses seizures in both pentylenetetrazole- and kainic-acid-induced mouse models. Mechanistically, B. fragilis activates colonic choline acetyltransferase-positive (ChAT + ) cells and enhances gut-vagus-brain cholinergic signaling, as demonstrated by vagal recordings, pharmacological blockade, and chemogenetic manipulation, identifying a colonic ChAT + -nodose ganglion circuit mediating seizure suppression. Its antiseizure effects associate with enriched intestinal Lactobacillus colonization. A randomized clinical trial (CHiCTR2100042203) further confirms the therapeutic efficacy of B. fragilis in pediatric refractory epilepsy. These findings define a gut-brain cholinergic pathway through which B. fragilis exerts antiseizure effects and establish a mechanistic basis for microbiota-targeted therapies in epilepsy.
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DOI
PubMed
高影响因子
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