Arabian Journal of Chemistry
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2022
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IF 6.212
Ihsan Safwan Kamarazaman,Nor Azah Mohamad Ali,Fauziah Abdullah,Nazrin Che Saad,Aida Azlina Ali,Salfarina Ramli,Pornchai Rojsitthisak,Hasseri Halim
Occurrence of the wound or chronic wound, which results to disability, amputation and diminish quality of life, are leading to increased healthcare expenditure around the globe. Despite effective conventional wound healing medicines, the exploration of alternative medicines are continuing process as researchers seek for the approach to reduce the cost of wound healing management. In present study, wound healing properties of ethanolic extract of Baeckea frutescens leaves were determined by evaluating their cytotoxicity, proliferation and migration rate on two types of cells, keratinocytes (HaCaT) and fibroblasts (BJ). Furthermore, the antioxidant properties of this plant were determined by DPPH scavenging, ferric reducing antioxidant power (FRAP) and total phenolics content (TPC) assays. The phytochemistry of the extract was evaluated by phytochemicals screening and liquid chromatography mass spectrometry (LCMS) analysis. Results of this study indicated Baeckea frutescens extract increased the rate of proliferation and migration on both HaCat and BJ cells within their nontoxic doses. The extract also possessed a very good antioxidant property as demonstrated with high DPPH radical scavenging, FRAP and TPC values, comparable to that of Green tea extract, a widely known antioxidant. The phytochemistry analyses and LCMS exhibited the presence saponins, flavonoids, tannins and steroids in Baeckea frutescens extract possibly responsible to their antioxidant and wound healing properties.
研究领域:
药理学
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植物化学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2021
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IF 5.645
Ning Wan,Ping Kou,Hong-yi Pang,Yuan-hang Chang,Liu Cao,Chang Liu,Chun-jian Zhao,Cheng-bo Gu,Yu-jie Fu
This work was designed to develop an integrated method of ultrasonic-microwave assisted extraction with enzyme pre-treatment and surfactant aqueous (ES-UME) for the simultaneous extraction of essential oil and flavonoids from Baeckea frutescens . The optimal parameters confirmed by single factor optimization combined with Box-Behnken Design were as follows: Cellulase and Hemicellulase concentration 10% (w/v), Triton X-114 concentration 2% (w/v), extraction time 1.5 h, ultrasonic power 478 W, microwave power 535 W, and liquid/solid ratio 16 mL/g. Under the optimal parameters, the extraction yields of essential oil and main five representative flavonoids epicatechin , rutin , isoquercitrin , myricetin , and quercetin reached 6.38, 0.53, 18.58, 10.72, 1.68, and 2.11 mg/g, respectively. Compositional analysis revealed α -Pinene (16.13%), Linalool (15.00%), 4-Carvomenthenol (10.98%), β -Caryophyllene (6.52%), and α -Terpineol (5.43%) were the most prominent components of essential oil. Furthermore, the essential oil exhibited broad-spectrum antimicrobial activity against 7 tested strains and excellent antioxidant activity through DPPH (2, 2-diphenyl-1-picrylhydrazyl), ABTS (2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and antibacterial assays. Moreover, flavonoids extracts were preconcentrated by 9.70-fold compared with the original extracts using the cloud-point property of surfactants. Overall, the developed method was a potential and desirable alternative that can simultaneously extract volatile and non-volatile bioactive constituents from plant materials in food , healthy, and pharmaceutical industries.
研究领域:
食品科学
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药学
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化学工程
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植物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）