JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
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2024
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IF 1.1
Zhenni Ji,Chang Liu,Fa Xu,Yi Wang,Dongyu Gu,Yun Huang,Yi Yang
Blaps rynchopetera Fairmaire is a medicinal insect with a wide range of biological activities. Some of its activities, including anti-cancer and anti-inflammatory activities, are closely related to antioxidant capacity. In this study, a method was established to investigate components and theoretical mechanism of antioxidant from B. rynchopetera . As a result, two antioxidants were obtained by bioassay-guided separation and identified as 4-(2-hydroxyethyl)-1,2-benzenediol and 4-ethylbenzol-1,3-diol. Their EC 50 were 20.20 ± 0.30 μM and 22.90 ± 1.30 μM for ABTS· + scavenging, and 153.00 ± 2.00 μM and 301.00 ± 3.00 μM for DPPH· scavenging, respectively. The thermodynamic investigation indicated that the 2-OH of 4-(2-hydroxyethyl)-1,2-benzenediol and the 1-OH of 4-ethylbenzol-1,3-diol were more susceptible to be attacked by free radicals. They tended to exert antioxidant effects via the sequential proton loss electron transfer mechanism in polar media, whereas the hydrogen atom transfer mechanism was more likely to occur in non-polar media. In addition, frontier molecular orbital theory and energy barrier analysis suggested that 4-(2-hydroxyethyl)-1,2-benzenediol was more likely to react with DPPH·. These results confirmed that 4-(2-hydroxyethyl)-1,2-benzenediol had better antioxidant potential.
研究领域:
昆虫学
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药理学
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生物化学
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产品关联
DOI
高影响因子
Chinese Journal of Integrative Medicine
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2023
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IF 2.9
Qian Xiao-li,Meng Di,Liu Heng,Liu Chao-he,Zhou Ping,Yang Yin-he,Wang Jia-peng,Xiao Huai,Ding Zhong-tao
Objective To explore the proliferation inhibitory effect of quinones from Blaps rynchopetera defense secretion on colorectal tumor cell lines. Methods Human colorectal cancer cell HT-29, human colorectal adenocarcinoma cell Caco-2 and normal human colon epithelial cell CCD841 were chosen for the evaluation of inhibitory activity of the main quinones of B. rynchopetera defense secretion, including methyl p-benzoquinone (MBQ), ethyl p-benzoquinone (EBQ), and methyl hydroquinone (MHQ), through methyl thiazolyl tetrazolium assay. The tumor-related factors, cell cycles, related gene expressions and protein levels were detected by enzyme-linked immunosorbent assy, flow cytometry, RT-polymerase chain reaction and Western blot, respectively. Results MBQ, EBQ, and MHQ could significantly inhibit the proliferation of Caco-2, with half maximal inhibitory concentration (IC 50 ) values of 7.04 ± 0.88, 10.92 ± 0.32, 9.35 ± 0.83, HT-29, with IC 50 values of 14.90 ± 2.71, 20.50 ± 6.37, 13.90 ± 1.30, and CCD841, with IC 50 values of 11.40 ± 0.68, 7.02 ± 0.44 and 7.83 ± 0.05 µg/mL, respectively. Tested quinones can reduce the expression of tumor-related factors tumor necrosis factor α, interleukin (IL)-10, and IL-6 in HT-29 cells, selectively promote apoptosis, and regulate the cell cycle which can reduce the proportion of cells in the G 1 phase and increase the proportion of the S phase. Meanwhile, tested quinones could up-regulate mRNA and protein expression of GSK-3β and APC, while down-regulate that of β-catenin, Frizzled1, c-Myc, and CyclinD1 in the Wnt/β-catenin pathway of HT-29 cells. Conclusion Quinones from B. rynchopetera defense secretion could inhibit the proliferation of colorectal tumor cells and reduce the expression of related factors, which would be functioned by regulating cell cycle, selectively promoting apoptosis, and affecting Wnt/β-catenin pathway-related mRNA and protein expressions.
研究领域:
肿瘤学
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药理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
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