INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Chaoyue Zhao,Long Huang,Jie Tang,Linlin Lv,Xinying Wang,Xiyao Dong,Fengrui Yang,Qingxiang Guan
The considerable challenge of wound healing remains. In this study, we fabricated a novel multifunctional core-shell nanofibrous scaffold named EGF@BSP-CeO 2 /PLGA (EBCP), which is composed of Bletilla striata polysaccharide (BSP), Ceria nanozyme (CeO 2 ) and epidermal growth factor (EGF) as the co re and poly(lactic-co-glycolic acid) (PLGA) as the shell via an emulsion electrospinning technique. An increase in the BSP content within the scaffolds corresponded to improved wound healing performance. These scaffolds exhibited increased hydrophilicity and porosity and improved mechanical properties and anti-UV properties. EBCP exhibited sustained release, and the degradation rate was <4 % in PBS for 30 days. The superior biocompatibility was confirmed by the MTT assay, hemolysis, and H&E staining. In addition, the in vitro results revealed that, compared with the other groups, the EBCP group presented excellent antioxidant and antibacterial effects. More importantly, the in vivo results indicated that the wound closure rate of the EBCP group reached 94.0 % on day 10 in the presence of H 2 O 2 . The results demonstrated that EBCP could comprehensively regulate the wound microenvironment, possess hemostatic abilities, and significantly promote wound healing. In conclusion, the EBCP is promising for facilitating the treatment of infected wounds and represents a potential material for clinical applications.
研究领域:
生物材料学
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医学
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组织工程
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
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2014
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IF 2.524
Jian-Wei Dong,Le Cai,Jie Xiong,Xiu-Hua Chen,Wei-Ying Wang,Ni Shen,Bei-Lei Liu,Zhong-Tao Ding
The tubers of Bletilla striata were fermented with Fusarium avenaceum and Fusarium oxysporum . The total phenolic contents (TPCs) and antioxidant and antibacterial activities of the extracts from the fermented products were measured. Additionally, the extracts of both F. avenaceum and F. oxysporum fermented B. striata (FBS) were characterized by UV–vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. The results showed that the F. avenaceum and F. oxysporum FBSs possessed higher TPCs and exhibited stronger antioxidant and antibacterial activities ( Bacillus subtilis ) in comparison with original material not co-fermented with microorganisms. Chemical characterization of the two extracts with UV–vis, FT-IR, and 1 H NMR implied that some of the phenolic glycosides may be deglycosylated and that bibenzyl compounds and saccharides may be metabolized in both FBS systems. Microbial fermentation enhances the TPC as well as the antioxidant and antibacterial activities of B. striata . The present study suggests that F. avenaceum and F. oxysporum fermentations were effective in B. striata processing.
研究领域:
植物学
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药理学
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微生物学
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DOI
高影响因子
BMC Complementary Medicine and Therapies
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2022
·Vol. 22
·期 1
·1-14
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IF 2.838
Luo Yiyuan,Wang Juan,Li Shuo,Wu Yue,Wang Zhirui,Chen Shaojun,Chen Hongjiang
Background Bletilla striata is the main medicine of many skin whitening classic formulas in traditional Chinese medicine (TCM) and is widely used in cosmetic industry recently. However, its active ingredients are still unclear and its fibrous roots are not used effectively. The aim of the present study is to discover and identify its potential anti-melanogenic active constituents by zebrafish model and molecular docking. Methods The antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2′-azino-bis-(3-ethylbenthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay . The anti-melanogenic activity was assessed by tyrosinase inhibitory activity in vitro and melanin inhibitory in zebrafish. The chemical profiles were performed by ultra-high-performance liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Meanwhile, the potential anti-melanogenic active constituents were temporary identified by molecular docking. Results The 95% ethanol extract of B. striata fibrous roots (EFB) possessed the strongest DPPH, ABTS, FRAP and tyrosinase inhibitory activities, with IC 50 5.94 mg/L, 11.69 mg/L, 6.92 mmol FeSO 4 /g, and 58.92 mg/L, respectively. In addition, EFB and 95% ethanol extract of B. striata tuber (ETB) significantly reduced the melanin synthesis of zebrafish embryos in a dose-dependent manner. 39 chemical compositions, including 24 stilbenoids were tentatively identified from EFB and ETB. Molecular docking indicated that there were 83 (including 60 stilbenoids) and 85 (including 70 stilbenoids) compounds exhibited stronger binding affinities toward tyrosinase and adenylate cyclase. Conclusion The present findings supported the rationale for the use of EFB and ETB as natural skin-whitening agents in pharmaceutical and cosmetic industries.
研究领域:
药理学
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化妆品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS RESEARCH
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2023
·Vol. 38
·期 9
·2495-2505
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IF 2.7
Hu Zhengbo,Zhao Kai,Zhou Fangmei,Ye Xiaoqing,Chen Yuchi,Jin Lixia,Ruan Xinming,Ding Zhishan,Zhu Bingqi
The purpose of this study is to utilize the active ingredients of Bletilla striata ( B. striata ) to prepare the hydrogel dressing for accelerated wound healing. To this end, a series of composite hydrogels were prepared by blending different proportions of B. striata polysaccharide (BSP) and ethanol extract of B. striata (EEB) with Carbomer 940 (CBM940). These hydrogels showed porous three-dimensional structures with good water retention properties. The cytotoxicity test demonstrated the high cytocompatibility of the EEB/BSP hydrogels. Additionally, the inclusion of EEB enhanced the antibacterial and anti-inflammatory properties of EEB/BSP hydrogel. The animal experiment illustrated the accelerated wound healing effect using the hydrogels and the highest healing rate was obtained in EEB1/BSP7 (m EEB :m BSP = 1:7) group with the 82.00 ± 4.25% and 93.30 ± 0.97% on 7th day and 10th day, respectively. Histological observations further confirmed the excellent healing effect of EEB/BSP hydrogel. Therefore, EEB/BSP hydrogel had potential to becoming a suitable wound dressing.
Graphical abstract
研究领域:
药剂学
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材料科学
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生物医学工程
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伤口愈合
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Guanghui Jiang,Haoyue Kong,Yuwen Wang,Yufei Wang,Junjie Zhou,Zhezhi Wang,Junfeng Niu
Liver fibrosis is a significant precursor to cirrhosis and liver cancer, yet effective treatments remain elusive. Our previous studies demonstrated that Bletilla striata polysaccharide (BSP) has therapeutic effects, though it performed poorly at medium concentrations. To address this limitation, BSP underwent selenization modification. In this study, selenized BSP (Se-BSP) was structurally characterized, and its in vivo activity in alleviating liver fibrosis was evaluated. The results showed that the molecular weight of Se-BSP increased, its in vitro antioxidant activity improved, and it exhibited enhanced efficacy in alleviating liver fibrosis at medium concentrations (150 mg/kg) compared to BSP. These findings provide a theoretical basis for the potential application of Se-BSP as an anti-liver fibrosis agent.
研究领域:
药理学
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生物化学
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肝脏疾病研究
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产品关联
DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
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2025
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IF 2.6
Yilong Wei,Yanan Jiang,Doudou Ma,Li Luo,Jiandong Ma,Xiaoliang Zhao,Yonggang Wang,Jing Zhang,Cunjin Wang,Weijie Zhang
Photocrosslinkable injectable hydrogels dressings prepared based on natural polymer materials not only have good biocompatibility and bioactivity, but also possess the ability to fill tissue defects of irregular shapes and the characteristics of non - invasive drug delivery, showing great research value in the biomedical field. However, it remains a challenge to prepare photocrosslinkable injectable hydrogels dressings with better performance by using natural materials. Therefore, the effects of both radical photo polymerization and photo-click chemistry method on the properties of hydrogels were explored in depth in this paper. A series of radical photo polymerization hydrogels and photo - click chemistry hydrogels were prepared under visible light, with natural Bletilla striata polysaccharide and hyaluronic acid as base material. Comparison of the two types of hydrogels revealed that photo - click chemistry hydrogels have more uniform and dense pore sizes, more superior mechanical properties, better cell compatibility, stronger antioxidant properties, as well as good injectability, drug - sustained - release performance and degradability. The results show that photocrosslinked hydrogels dressings prepared by photo - click chemistry method have better properties than radical photo polymerization method, and exhibit greater potential for application in biomedical fields such as wound healing.
研究领域:
生物材料学
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组织工程
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药物递送
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产品关联
DOI
高影响因子
Inorganics
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2023
·Vol. 11
·期 1
·40
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IF 2.9
Jiahao Zhang,Yunxue Yin,Shuo Hu,Guangyu Wang,Yuxin Tong,Mingyang Zen,Zhile Liang,Minghao Li,Ran Yan,Yue Wang
The silver nanoparticle is a good antibacterial material being used as a broad-spectrum fungicide, including against some multidrug-resistant strains. Compared with the normal chemical and physical preparation methods, green synthesis has attracted wide attention, because of the pharmaceutical activities of the natural product, mild reaction conditions, and environmentally friendly, etc. In this study, the synthesis of silver nanoparticles (Ag NPs) was prepared fromBletilla striatapolysaccharide (BSP) and characterized by UV-vis spectroscopy and Dynamic Light Scattering (DLS). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) indicated the morphology of Ag NPs was subspherical with an average size of 20–35 nm.Bletilla striatapolysaccharide not only can be used as a natural reducing agent, but also has good repairing ability. Moreover, the antibacterial experimental results showed its great antimicrobial activity against Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Escherichia coli) andCandida albicans.Keywords:silver nanoparticles;green synthesis;Bletilla striatapolysaccharide;antibacterial
研究领域:
纳米技术
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药学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Junjie Qiu,Xiao Xu,Jingyan Guo,Zhenyu Wang,Jinjin Wu,Huiqin Ding,Yuchen Xu,Yili Wu,Qianyi Ying,Jiawei Qiu,Suxiang Wu,Senlin Shi
We optimized the extraction process of Bletilla striata polysaccharides using orthogonal design, Box-Behnken design (BBD), and genetic algorithm-back propagation (GA-BP), then compared and evaluated them to confirm that the combination of BBD and GA-BP neural networks was capable of increasing polysaccharide yields and antioxidant activity. The optimal extraction parameters were as follows: liquid-to-solid ratio of 15 mL/g, extraction power of 450 W, and extraction time of 34 min. Under these conditions, the polysaccharide yield and antioxidant activity were 8.29 ± 0.50 % and 26.20 ± 0.28 (mM FE/mg). Subsequently, the polysaccharide was purified to obtain purified Bletilla striata polysaccharides 1 (pBSP1) with a Mw of 255.172 kDa. Scanning electron microscope (SEM), ultraviolet-visible detector (UV), fourier transform infrared spectrometer (FTIR), high performance liquid chromatography (HPLC), X-ray diffraction (XRD), nuclear magnetic resonance (NMR) and periodate oxidation were used to analyze the structure of pBSP1. The results showed pBSP1 had a smooth surface and a rough interior, with a composition of α-D conformation glucose (18.23 %) and β-D conformation mannose (53.77 %), and an amorphous crystal structure. According to the results of thermogravimetric and rheological tests, pBSP1 exhibits good thermal stability and viscoelastic behavior. Furthermore, pBSP1 protected lipopolysaccharide (LPS)-induced GES − 1 and Caco2 cells, the results showed pBSP1(400 μg/mL) lowered TEER synthesis in Caco2 cells as well as apoptosis and reactive oxygen species (ROS) production in both cells, indicating that pBSP1 may have an intestine protective effect.
研究领域:
药理学
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生物化学
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食品科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Natural Products and Bioprospecting
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2026
·Vol. 16
·期 1
·73
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IF 5.8
Li Xuan,Gu Qinghui,Hu Qile,Deng Tuo,Luo Lan,Cai Jianping,Dong Xudong,Wu Chunhua,Wu Mingyi
Bletilla striata is highly regarded in traditional Chinese medicine and has also gained widespread attention as a valuable medicinal plant resource. However, the molecular composition and bioactive potential of its polysaccharides present a significant knowledge gap. Herein, a specific glucomannan fraction, designated as BSP60, was purified from Bletilla striata tubers and subsequently subjected to structural, rheological, and biological evaluations. Comprehensive characterization via methylation-GC–MS, monosaccharide composition analysis, and 1D and 2D NMR techniques demonstrated that BSP60 possesses a β-(1 → 4)-linked backbone featuring alternating mannosyl and glucosyl units, with the mannose moieties exhibiting partial O -acetylation at their C-2 or C-3 positions (with an acetylation rate of 23.54%). Rheological studies showed that BSP60 aqueous solutions exhibit shear-thinning behavior and form an elastic-dominated viscoelastic network, with higher viscosity and storage modulus than dextran of comparable molecular weight, indicating enhanced intermolecular interactions. Biological assays revealed that the BSP60 effectively decreased the level of NO, IL-6, and TNF-α in LPS-triggered macrophages (RAW264.7). Furthermore, the scavenge ability of BSP60 to superoxide anion (O 2 •− ) and ABTS ⁺• radicals are significantly higher than that of hyaluronic acid. This study systematically characterizes the structural features of glucomannan derived from Bletilla striata , thereby establishing a robust scientific basis for its expanded utilization in the development of biomedical materials and functional cosmetic ingredients.
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2021
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IF 5.811
Zhao Yan,Wang Qibin,Yan Shan,Zhou Jun,Huang Liangyong,Zhu Haitao,Ye Fang,Zhang Yonghong,Chen Lin,Chen Li,Zheng Tao
The aim of this study was to evaluate the therapeutic effects of Bletilla striata polysaccharide (BSP) on wound healing in diabetes mellitus (DM) and to explore the underlying mechanisms. DM mouse models were induced by high fat-diet feeding combined with low-dose streptozocin injection. To establish diabetic foot ulcer (DFU) models, DM mice were wounded on the dorsal surface. Subsequently, mice were treated with vehicle or BSP for 12 days and wound healing was monitored. The effects of BSP on the production of interleukin-1β (IL-1β), tumor necrosis factor-α, macrophages infiltration, angiogenesis, the activation of nucleotide-binding and oligomerization (NACHT) domain, leucine-rich repeat (LRR), and pyrin domain (PYD)-containing protein 3 (NLRP3) inflammasome, and insulin sensitivity in wound tissues were subsequently evaluated. Separated- and cultured- bone marrow-derived macrophages (BMDMs) and cardiac microvascular endothelial cells (CMECs) were isolated from mice and used to investigate the effects of BSP on cell viability, reactive oxygen species (ROS) generation, NLRP3 inflammasome activation and insulin sensitivity in vitro following exposure to high glucose (HG). BSP administration accelerated diabetic wound healing, suppressed macrophage infiltration, promoted angiogenesis, suppressed NLRP3 inflammasome activation, decreased IL-1β secretion, and improved insulin sensitivity in wound tissues in DM mice. In vitro, co-treatment with BSP protected against HG-induced ROS generation, NLRP3 inflammasome activation, and IL-1β secretion in BMDMs, and improved cell viability and decreased ROS levels in CMECs. Moreover, in HG exposed BMDMs-CMECs cultures, BSP treatment suppressed NLRP3 inflammasome activation and IL-1β secretion in BMDMs, and improved cell viability and insulin sensitivity in CMECs. Furthermore, treatment with IL-1β almost completely suppressed the beneficial effects of BSP on the NLRP3 inflammasome, IL-1β secretion, and insulin sensitivity in HG-treated BMDMs-CMECs. BSP promotes DFU healing through inhibition of the HG-activated NLRP3 inflammasome.
研究领域:
药理学
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免疫学
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内分泌学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yirui Wang,Shaowei Han,Ruofei Li,Baosong Cui,Xianjie Ma,Xinzhu Qi,Qi Hou,Mingbao Lin,Jinye Bai,Shuai Li
Two water-soluble polysaccharides , BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata . The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively. The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively. The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d ‑mannosyl residues and β‑1,4‑linked d ‑glucosyl residues. BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
研究领域:
植物化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2025
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IF 4.7
Xinyu Gao,Xinjiani Chen,Suxian Song,Changle Lu,Zhen Zhang,Yu Zhou,Liping Yao,Xiaojun Liu,Rongqing Zhang
Bletilla striata polysaccharide (BSP) is effective at healing wounds and has important application value in the research and development of biomedical materials. In this study, BSP, chitosan, and sodium β-glycerophosphate were used to generate a complex hydrogel with a very small pore size of 10–30 μm without the use of cross-linking agents. By improving the cross-linking density, the mechanical property defects caused by excessive BSP dissolution were overcome without affecting its physiological activity. Moreover, the small molecule Danshen sodium (SDSS) was loaded into the three-dimensional network of this hydrogel to form a hydrogel drug carrier system. SDSS could be released in the long term, and the total amount released after 53 h was 96.26 ± 2.57%. The BSP hydrogel had good water absorption (169.47 ± 4.54%) and bonding properties. In vitro studies confirmed that it has a good antibacterial performance and biocompatibility and the ability to promote cell proliferation (>200%) and migration. Molecular experiments confirmed that the hydrogel promotes collagen expression. In vivo experiments using a mouse wound healing model confirmed that the hydrogel has an excellent ability to promote wound healing, particularly during the first 7 days of the wound. The wound healing rate of the hydrogel group was higher than that of the blank group by 27.61% (p < 0.05), and the effect of the hydrogel to promote wound healing was confirmed by wound tissue staining.
研究领域:
生物医学材料
·
药剂学
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）