FOOD SCIENCE AND BIOTECHNOLOGY
·
2017
·Vol. 26
·期 1
·143-152
·
IF 0.699
Chen Peng,Yan Lei,Zhang Shuang,Wu Zhengrong,Li Suyue,Yan Xiaojuan,Wang Ningbo,Liang Ning,Li Hongyu
A stirring bioreactor packed with a carbon fiber textiles (FT) biofilm formed by Bacillus subtilis was used to produce vanillin from ferulic acid. Biofilm formation was characterized by scanning electron microscopy. The interactive effects of three variables on vanillin molar yield (M) and conversion efficiency of ferulic acid (E) were evaluated by response surface methodology (RSM) with a Box-Behnken design (BBD). The optimal conversion conditions with a maximum overall desirability D of 0.983 were obtained by a desirability function. Considering the actual operation, the confirmation tests were performed using the slightly modified optimal conditions (initial ferulic acid concentration 1.55 g/L, temperature 35°C, stirring speed 220 rpm). The results showed that M and E were 57.42 and 93.53%, respectively. This was only 1.03% and 1.87%, respectively, different from the predicted values, confirming the validity of the predicted models. These revealed that the stirred packed reactor could be successfully used in vanillin bioconversion from ferulic acid.
研究领域:
生物工程
·
微生物学
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DOI
PubMed
高影响因子
Scientific Reports
·
2023
·Vol. 13
·期 1
·1-14
·
IF 4.6
Alishiri Mahsa,Gonbadi Maryam,Narimani Mehdi,Abdollahi Seyyed Amirreza,Shahsavaripour Negin
The contamination of the aquatic environment with antibiotics is among the major and developing problems worldwide. The present study investigates the potential of adsorbent magnetite-chitosan nanoparticles (Fe 3 O 4 /CS NPs) for removing trimethoprim (TMP) and sulfamethoxazole (SMX). For this purpose, Fe 3 O 4 /CS NPs were synthesized by the co-precipitation method, and the adsorbent characteristics were investigated using XRD, SEM, TEM, pH zpc , FTIR, and VSM. The effect of independent variables (pH, sonication time, adsorbent amount, and analyte concentration) on removal performance was modeled and evaluated by Box–Behnken design (BBD). The SEM image of the Fe 3 O 4 /CS adsorbent showed that the adsorbent had a rough and irregular surface. The size of Fe 3 O 4 /CS crystals was about 70 nm. XRD analysis confirmed the purity and absence of impurities in the adsorbent. TEM image analysis showed that the adsorbent had a porous structure, and the particle size was in the range of nanometers. In VSM, the saturation magnetization of Fe 3 O 4 /CS adsorbent was 25 emu g −1 and the magnet could easily separate the adsorbent from the solution. The results revealed that the optimum condition was achieved at a concentration of 22 mg L −1 , a sonication time of 15 min, an adsorbent amount of 0.13 g/100 mL, and a pH of 6. Among different solvents (i.e., ethanol, acetone, nitric acid, and acetonitrile), significant desorption of TMP and SMX was achieved using ethanol. Also, results confirmed that Fe 3 O 4 /CS NPs can be used for up to six adsorption/desorption cycles. In addition, applying the Fe 3 O 4 /CS NPs on real water samples revealed that Fe 3 O 4 /CS NPs could remove TMP and SMX in the 91.23–95.95% range with RSD (n = 3) < 4. Overall, the Fe 3 O 4 /CS NPs exhibit great potential for removing TMP and SMX antibiotics from real water samples.
研究领域:
环境科学
·
材料科学
·
药学
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产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
·
2016
·
IF 1.566
Wenqiang Ba,Zhou Li,Lisheng Wang,Ding Wang,Weiguo Liao,Wentao Fan,Yinai Wu,Fengyun Liao,Jianye Yu
The purpose of the present study was to prepare and optimize sinomenine (SIN) pluronic lecithin organogels system (PLO), and to evaluate the permeability of the optimized PLO in vitro and in vivo. Box–Behnken design was used to optimize the PLO and the optimized formulation was pluronic F127 of 19.61%, lecithin of 3.60% and SIN of 1.27%. The formulation was evaluated its skin permeation and drug deposition both in vitro and in vivo compared with gel. Permeation and deposition studies of PLO were carried out with Franz diffusion cells in vitro and with microdialysis in vivo. In vitro studies, permeation rate (Jss) of SIN from PLO was 146.55 ± 2.93 μg/cm2/h, significantly higher than that of gel (120.39 μg/cm2/h) and the amount of SIN deposited in skin from the PLO was 10.08 ± 0.86 μg/cm2, significantly larger than that from gel (6.01 ± 0.04 μg/cm2). In vivo skin microdialysis studies showed that the maximum concentration (Cmax) of SIN from PLO in “permeation study” and “drug-deposition study” were 150.27 ± 20.85 μg/ml and 67.95 μg/ml, respectively, both significantly higher than that of SIN from gel (29.66 and 6.73 μg/ml). The results recommend that PLO can be used as an advantageous transdermal delivery vehicle to enhance the permeation and skin deposition of SIN.
研究领域:
药剂学
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药物递送系统
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Baogui Ye,Zhongwei Zhao,Hongmei Liu
Construction of multi-interface contact step-scheme photocatalyst is a promising pathway to achieve high-electron transfer efficiency for photodegradation Estrone and Congo red (CR). In this work, g-C 3 N 4 was selected as the main component, CeO 2 nanosheets with unique Ce 4+ →Ce 3+ conversion property and M-rGO were loaded on the surface of g-C 3 N 4 to construct magnetic photocatalyst, whose structure imparted reusability by magnetic retrieval and at the same time M-rGO acted as an efficient host for g-C 3 N 4 /CeO 2 composite photocatalyst. The photocatalytic performances were investigated with Estrone and CR, and the results showed that the composite photocatalyst g-C 3 N 4 /CeO 2 /M-rGO could degrade 71% of Estrone and 92% of CR. Free radical scavenger test showed that superoxide and hydroxyl radical species played a major role in the degradation. Furthermore, it was demonstrated that the magnetic photocatalyst g-C 3 N 4 /CeO 2 /M-rGO possessed strong antibacterial properties against Proteus mirabilis. A three-factor-three-level Box-Behnken design (BBD) was selected to optimize three greatly influential parameters: light irradiation time (min), the mass of photocatalyst (mg) and pollution concentration (ppm) by responding surface methodology. It was expected that the g-C 3 N 4 /CeO 2 /M-rGO can be used as a magnetic photocatalyst to achieve its valorization, which has very broad development prospects in the field of environmental remediation or catalysis.
研究领域:
环境科学
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材料科学
·
生物学
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产品关联
DOI
高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 7
·1330
·
IF 6.525
Yan Zhang,Runan Zuo,Xinhao Song,Jiahao Gong,Junqi Wang,Mengjuan Lin,Fengzhu Yang,Xingxing Cheng,Xiuge Gao,Lin Peng,Hui Ji,Xia Chen,Shanxiang Jiang,Dawei Guo
Maduramicin ammonium (MAD) is one of the most frequently used anticoccidial agents in broiler chickens. However, the high toxicity and low solubility of MAD limit its clinical application. In this study, MAD-loaded nanostructured lipid carriers (MAD–NLCs) were prepared to overcome the defects of MAD by using highly soluble nanostructured lipid carriers (NLCs). The formulation was optimized via a three-level, three-factor Box–Behnken response surface method. Then, the optimal MAD–NLCs were evaluated according to their hydrodynamic diameter (HD), zeta potential (ZP), crystal structure, encapsulation efficiency (EE), drug loading (DL), in vitro release, and anticoccidial effect. The optimal MAD–NLCs had an HD of 153.6 ± 3.044 nm and a ZP of −41.4 ± 1.10 mV. The X-ray diffraction and Fourier-transform infrared spectroscopy results indicated that the MAD was encapsulated in the NLCs in an amorphous state. The EE and DL were 90.49 ± 1.05% and 2.34 ± 0.04%, respectively, which indicated that the MAD was efficiently encapsulated in the NLCs. In the in vitro study, the MAD–NLCs demonstrated a slow and sustained drug release behavior. Notably, MAD–NLCs had an excellent anticoccidial effect againstEimeria tenellain broiler chickens. In summary, MAD–NLCs have huge potential to form a new preparation administered via drinking water with a powerful anticoccidial effect.Keywords:maduramicin ammonium;nanostructured lipid carriers;Box–Behnken design;anticoccidial effect
研究领域:
兽医学
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药剂技术
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动物健康
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2016
·
IF 4.556
Fengli Chen,Qiang Zhang,Shimin Fei,Huiyan Gu,Lei Yang
In this study, ultrasonic circulating extraction (UCE) technique was firstly and successfully applied for extraction of samara oil from Acer saccharum . The extraction kinetics were fitted and described, and the extraction mechanism was discussed. Through comparison, n -hexane was selected as the extraction solvent, the influence of solvent type on the responses was detailedly interpreted based on the influence of their properties on the occurrence and intensity of cavitation. Seven parameters potentially influencing the extraction yield of samara oil and content of nervonic acid, including ultrasound irradiation time, ultrasound irradiation power, ultrasound temperature, liquid-solid ratio, soaking time, particle size and stirring rate, were screened through Plackett–Burman design to determine the significant variables. Then, three parameters performed statistically significant, including liquid-solid ratio, ultrasound irradiation time and ultrasound irradiation power, were further optimized using Box–Behnken design to predict optimum extraction conditions. Satisfactory yield of samara oil (11.72 ± 0.38%) and content of nervonic acid (5.28 ± 0.18%) were achieved using the optimal conditions. 1% proportion of ethanol in extraction solvent, 120 °C of drying temperature and 6.4% moisture were selected and applied for effective extraction. There were no distinct differences in the physicochemical properties of samara oil obtained by UCE and Soxhlet extraction, and the samara oil obtained by UCE exhibited better antioxidant activities. Therefore, UCE method has enormous potential for efficient extraction of edible oil with high quality from plant materials.
研究领域:
食品科学与技术
·
植物化学工程
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Jixian Zhang,Xinglin Zeng,Guoyan Liu,Chaoting Wen,Xin Xu
Ultrasound extraction (UE) enhanced with deep eutectic solvent (DES) was used to extract Lentinus edodes polysaccharides. Box-Behnken design (BBD) was applied to investigate the influences of water content (10-90%), solid-liquid solvent (1:10-1:50 g/mL), time (4-12 min), temperature (40-80 °C) and ultrasonic power (100-500 W) on the yield of Lentinus edodes polysaccharides. The optimal extraction conditions were ultrasonic power of 300 W, extraction time of 8 min, water content of 80%, a solid-liquid ratio of 1:30 g/mL and a temperature of 60 °C, respectively. The highest extraction yield of Lentinus edodes polysaccharide was 10.17% under optimal conditions. The results of FT-IR, SEM, and monosaccharide composition confirmed that the extracts possessed the characteristics of polysaccharides. In addition, the polysaccharides obtained with the UE enhanced with DES method exhibited higher antioxidant activities than the polysaccharides extracted with the UE method and HWE method. This extraction method can further expand the production efficiency and structural diversity of Lentinus edodes polysaccharides and meet the supply and demand relationship. It can be foreseen that this method can be applied to the extraction of more active substances.
研究领域:
食品科学
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药学
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生物技术
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 79
·42202-42210
·
IF 3.708
Xiao-Ying Qv,Yan-Yun Guo,Jian-Guo Jiang
Bio-energy is arousing the common interest of worldwide researchers to investigate it as a substitute for fossil resources. Compared to other bio-energy sources, algae energy has many advantages. This research was aimed to increase the lipid productivity of Dunaliella tertiolecta, an oleaginous microalgae, by optimizing algal culture medium. Plackett–Burman Design (PBD) was adopted to screen out the critical factors from seven nutritional elements. Box–Behnken design (BBD) was used to seek the optimal nutritional conditions for achieving maximum lipid productivity. Results showed that four nutrients, i.e. NaNO3, NaCl, NaHCO3 and NaH2PO4 2H2O, played a critical role in the accumulation of lipid. GC analysis verified that the four critical nutrients mentioned above had different influences on fatty acid methyl esters, moreover, NaCl had the most prominent effects on fatty acid composition of D. tertiolecta. NaCl 87.69 g L−1, NaNO3 1.77 g L−1, NaH2PO4·2H2O 1.92 g L−1, NaHCO3 5.57 g L−1 were proven to be the optimal culture conditions, under which the lipid productivity in the experiment was 12.576 ± 0.12 mg per L per day, which is approaching the theoretically predicted lipid productivity of 12.925 mg per L per day. The lipid productivity was increased by 31.4% under the optimal culture conditions compared to that under the initial medium composition, indicating that optimization of nutrients could effectively improve the lipid production of D. tertiolecta.
研究领域:
生物能源
·
微藻研究
·
生物技术
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DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2021
·
IF 5.816
Zhang Tianwei,Liu Xiangchen,Wang Guofeng,Liu Hao,Xiao Lei,Zhou Yihao,Liang Qiang,Zhang Cunwei
Combustion characteristics of hydrogen-containing syngas are distinct from those of traditional hydrocarbon fuels. However, the efficiency for any fire extinguishant on syngas/air flame suppression has not been validated up to now. In this study, we developed novel core-shell microstructured nanocomposites (CSMNs) with inner seawater for syngas/air fire extinguishing. To optimize the preparation conditions, we adopted a three-factor three-level Box-Behnken design (BBD). The corrosion rate and minimum extinguishing concentration of CSMNs were determined by corrosion tests and Cup-burner experiments, respectively. Results showed that the BBD method exhibits good agreement between experimental data and fitted models. By applying numerical optimization , we determined that solid mass fraction, rotation speed, and rotation time in a range of 2–10%, 4000–8000 rpm, and 60–180 s, respectively, are the optimal preparation conditions for the CSMN preparation. The degree of corrosion rate and mass concentration in co-flow gaseous fuel/air fire suppression were reduced by more than 50% compared to an aqueous seawater solution. The updated methods can be used to study the fire inhibition efficiency of the emerging class of composite materials with the core-shell structure. This does not only provide solutions for syngas/air flame inhibition, but it also gives new insight into the application of seawater for fire extinguishment.
研究领域:
燃烧学
·
材料科学
✓ 已验证
DOI
高影响因子
Results in Chemistry
·
2025
·
IF 4.2
Lifeng Yang,Pinjin Zheng,Zexin Chen
The aim of this research is to explore new techniques for preparing a cross-linked three-dimensional network nanogel structure (CS-NPS) loaded with bifendate (DDB). The ionic cross-linking method was used to prepare CS-NPS from tripolyphosphate (TPP) and chitosan (CS), and DDB was then encapsulated in the nanogel structure to obtain DDB-CS-NPS. The preparation conditions were optimized through single-variable experiments and the Box–Behnken response surface method to obtain DDB-CS-NPS with good system stability, moderate particle size, and good encapsulation efficiency. It was found that when the TPP concentration was 1.5 mg/ml, the feed ratio was 3.8:1, and the ultrasound treatment time was 10 min and 53 s, the encapsulation efficiency predicted by the Box–Behnken response surface method could reach 89.38 %. A verification test showed that the actual average encapsulation efficiency of the DDB-CS-NPS reached 88.64 %, and the deviation between the predicted and the actual values was −0.8220 %. The average particle size of the samples was 644.8 nm, the polydispersity index was 0.301, and the zeta potential was 6.68 ± 1.29 mV. The results indicate that the proposed preparation process is feasible, the response surface test model can accurately predict the preparation condition area of DDB-CS-NPS, the deviation between the predicted encapsulation efficiency and the actual value is small, and the encapsulation efficiency meets the expected requirements. The DDB-CS-NPS obtained has a moderate particle size, a uniform distribution, and a relatively low potential. However, it may be prone to aggregation or coagulation, and so should be stored in a refrigerated condition.
研究领域:
药剂学
·
生物材料
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产品关联
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Saige Meng,Shuangqing Wang,Ming Guan Piao
The synergistic gastroretentive drug delivery systems are capable of delivering drugs with a narrow absorption window. The purpose of this study is to prepare furosemide hollow-bioadhesive microspheres to improve bioavailability. The emulsion solvent volatilization method was adopted, through Box-Behnken design, with polymer ratio (X 1 ), dosage (X 2 ), organic solvent volume (X 3 ) and PVA concentration (X 4 ) as independent variables, and drug loading (Y 1 ), encapsulation rate (Y 2 ), yield rate (Y 3 ) and 8 h floating rate (Y 4 ) were response variables. The interaction effects of the independent and response variables were explored. The optimized hollow-bioadhesive microspheres showed the in vitro floating rate of 93.33 ± 5.24% for 8 h, the in vitro retention rate of 90.67 ± 3.21%, and the sustained drug release of 93.79 ± 4.90% for 12 h. Compared with the hollow microspheres, the hollow-bioadhesive microspheres significantly increase the retention time in the stomach. The results of Differential Scanning Calorimetry , Fourier Transform InfraRed, and X-ray diffraction showed that furosemide had good biocompatibility with excipients. Compared with commercial tablets, the relative bioavailability of hollow-bioadhesive microspheres was increased by 163.84%, and the long-term therapeutic concentration was maintained. The safety evaluation proved the oral safety of microspheres. In conclusion, the safe and economical furosemide hollow-bioadhesive microsphere is a valuable preparation for development, which can improve clinical application.
研究领域:
药剂学
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药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 5
·923
·
IF 6.525
Ting Zhang,Xixi Li,Juewen Xu,Jingbao Shao,Meihong Ding,Senlin Shi
As a biopharmaceutics classification system (BCS) class IV drug, breviscapine (Bre) has low solubility in water, poor chemical stability, a short biological half-life and rapid removal from plasma. This paper prepared a Bre nanosuspension (Bre-NS) by an ultrasound-assisted anti-solvent precipitation method. Characterization of Bre-NS was studied using a Box–Behnken design concerning drug concentration in DMSO, an anti-solvent-to-solvent ratio, and sonication time. Under the optimized conditions of 170 mg/mL for the drug concentration, a 1:60 solvent-to-anti-solvent ratio, and a 9 min sonication time, the particle size of Bre-NS was 303.7 ± 7.3 nm, the polydispersity index was 0.178 ± 0.015, and the zeta potential was −31.10 ± 0.26 mV. Combined with the results from differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), and Fourier transform-infrared spectroscopy (FT-IR), the findings indicated that the crystal form and chemical structure of Bre-NS did not change during the entire process. The optimized formulation displayed good stability, increased solubility, and better in vitro release. Therefore, the results of this study can be a reference for the delivery system design of insoluble active components and effective parts in traditional Chinese medicine.Keywords:breviscapine;nanosuspension;Box–Behnken design
研究领域:
药学
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药物递送系统
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纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）