JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2023
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IF 8.7
Tao Zhang,Wanling Cai,Zuliang Chen
Bio-nanocomposites have attracted increasing research attention because they are able to integrate bio- and nano-related functions, and subsequently demonstrate potentially beneficial environmental applications. Here, a functional bionanomaterial based on Burkholderia cepacian (FZ) immobilized on GO/ZIF-8 was developed and used to remove malachite green (MG), with functions based on both biodegradation and adsorption. XRD and FTIR results showed that in situ production of GO/ZIF-8 by combining Zn 2+ in ZIF-8 with the carboxyl group on the GO surface, led to FZ immobilized in GO/ZIF-8 through covalent bonding. Zeta analysis showed that the surface of FZ and GO/ZIF-8 had different charges under pH = 9.12, suggesting immobilization also occurred via electrostatic action. BET results confirmed that the specific surface area of GO/ZIF-8 was much larger than that of GO and ZIF-8, but the reduced specific surface area of FZ@GO/ZIF-8 could be due to FZ loading on its surface. The efficiency of FZ@GO/ZIF-8 in the removal of MG reached 99% and furthermore retained good stability after five cycles. The efficiency in removing multiple ions in river water reached more than 80%, which is evidence strongly suggesting that FZ@GO/ZIF-8 is an environmental bionanomaterial with effective application potential.
研究领域:
环境科学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2018
·Vol. 19
·期 5
·1424
·
IF 3.687
Miaad Adnan,Kai Li,Jianhua Wang,Li Xu,Yunjun Yan
A hierarchical mesoporous zeolitic imidazolate framework (ZIF-8) was processed based on cetyltrimethylammonium bromide (CTAB) as a morphological regulating agent and amino acid (l-histidine) as assisting template agent.Burkholderia cepacialipase (BCL) was successfully immobilized by ZIF-8 as the carrier via an adsorption method (BCL-ZIF-8). The immobilized lipase (BCL) showed utmost activity recovery up to 1279%, a 12-fold boost in its free counterpart. BCL-ZIF-8 was used as a biocatalyst in the transesterification reaction for the production of biodiesel with 93.4% yield. There was no significant lowering of conversion yield relative to original activity for BCL-ZIF-8 when continuously reused for eight cycles. This work provides a new outlook for biotechnological importance by immobilizing lipase on the hybrid catalyst (ZIF-8) and opens the door for its uses in the industrial field.Keywords:adsorption method;biodiesel production;Burkholderia cepacialipase (BCL);hexahedral ZIF-8;hierarchical ZIF-8;surfactant
研究领域:
生物技术
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催化化学
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可再生能源
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DOI
PubMed
高影响因子
Catalysts
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2018
·Vol. 8
·期 1
·13
·
IF 3.465
Li Xu,Guli Cui,Caixia Ke,Yanli Fan,Yunjun Yan
A kind of pH-responsive particle was synthesized using modified pullulan polysaccharide. The synthesized particle possessed a series of merits, such as good dispersity, chemical stability and variability of particle size, making it a suitable carrier for enzyme immobilization. Then,Burkholderia cepacialipase (BCL), a promising biocatalyst in transesterification reaction, was immobilized on the synthesized particle. The highest catalytic activity and immobilization efficiency were achieved at pH 6.5 because the particle size was obviously enlarged and correspondingly the adsorption surface for BCL was significantly increased. The immobilization enzyme loading was further optimized, and the derivative lipase was applied in chiral resolution. Under the optimal reaction conditions, the immobilized BCL showed a very good performance and significantly shortened the reaction equilibrium time from 30 h of the free lipase to 2 h with a conversion rate of 50.0% andeesat 99.2%. The immobilized lipase also exhibited good operational stability; after being used for 10 cycles, it still retained over 80% of its original activity. Moreover, it could keep more than 80% activity after storage for 20 days at room temperature in a dry environment. In addition, to learn the potential mechanism, the morphology of the particles and the immobilized lipase were both characterized with a scanning electron microscope and confocal laser scanning microscopy. It was found that the enlarged spherical surface of the particle in low pH values probably led to high immobilized efficiency, resulting in the improvement of enantioselectivity activity in chiral resolution.Keywords:immobilization;Burkholderia cepacialipase;chiral resolution;1-phenylethanol;pullulan
研究领域:
生物化学
·
酶工程
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产品关联
DOI
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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2013
·Vol. 30
·期 4
·1251-1260
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IF 1.262
Zhou Gang,Shi Qing-shan,Ouyang You-sheng,Chen Yi-ben
Isothiazolones are used as preservatives in various modern industrial products. Although microorganisms that exhibit resistance towards these biocides have been identified, the underlying resistance mechanisms are still unclear. Therefore, we investigated the resistance properties of the following Burkholderia cepacia strains to Kathon (a representative of isothiazolones): a wild-type (WT) strain; a laboratory resistance strain (BC-IR) induced from WT; and an isolated strain (BC-327) screened from industrial contamination samples. The bacterial cell structure was disrupted by 50 μg ml −1 Kathon treatment. BC-IR and BC-327 did not display resistance in the presence of 1 ml L −1 Tween 80, 1 ml L −1 Triton X-100, 0.1 % sodium dodecyl sulfate or 1 mmol L −1 EDTA-2Na. Additionally, BC-IR and BC-327 exhibited lower relative conductivity from 10 to 180 min. The types as well as the levels of outer-membrane proteins (OMPs) were altered among WT, BC-IR and BC-327. Finally, the two Kathon-resistance strains BC-IR and BC-327 presented higher resistance capacity to H 2 O 2 . We measured the levels of peroxide-sensor genes and observed that the transcriptional activator oxyR , superoxide dismutase sod1 , sod2 , catalase cat1 and cat3 were all up-regulated under oxidative conditions for all strains. Taken together, OMPs and peroxide-sensor genes in B. cepacia contributed to isothiazolone resistance; However, the laboratory strain BC-IR exhibited a different resistance mechanism and properties compared to the isolated strain BC-327.
研究领域:
微生物学
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分子生物学
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工业生物技术
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Zhipan Wang,Baokai Ma,Cai Shen,Ling-Zhi Cheong
Methyl parathion is one of the most widely used pesticides in agricultural practices. It caused accumulation of acetylcholine and over-stimulation of receptors in synapses which eventually led to damage of the nervous system. Present study developed a direct, sensitive, rapid and reliable method for methyl parathion residues detection in vegetable samples. MOF nanofibers which demonstrated stable framework structure, good thermal/chemical stability, good electrochemical behavior, high porosity, surface area and pore volume was synthesized and used for fabrication of Burkholderia cepacia lipase (BCL)@MOF nanofibers biosensors. BCL@MOF nanofibers/chitosan/GCE biosensor demonstrated high sensitivity for methyl detection with a wide linear range (0.1–38 µM) and low limit of detection 0.067 µM. During the 3 weeks storage stability test at 4 °C, the fabricated biosensor demonstrated good reusability and excellent stability for methyl parathion detection with retainment of more than 80% of its initial response. When applied for detection of methyl parathion residues in vegetable samples, the BCL@MOF nanofibers/chitosan/GCE biosensors demonstrated good recovery rates.
研究领域:
环境科学
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分析化学
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农业科学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2020
·Vol. 193
·期 3
·717-729
·
IF 2.277
Zhang Jun,Tian Miao,Chen Xiaoyan,Lv Pengmei,Luo Wen,Wang Zhiyuan,Xu Jingliang,Wang Zhongming
Biodiesel biosynthesis with enzymatic transesterification is considered green, sustainable, and environmentally friendly method. Lipase from Burkholderia cepacia G63 has excellent catalytic properties in biodiesel production. Lipase chaperones promote secretion and folding of enzymes, thereby enhancing enzymatic activity. In the current study, heterologous co-expression of lipase (lipA) and chaperone (lipB) was achieved in Escherichia coli through codon optimization. The enzymatic activity of purified and renatured lipAB was 2080.23 ± 19.18 U/g at 50 °C and pH 8.0. Moreover, lipAB showed increased resistance to pH and temperature changes, and lipAB retained stable catalytic properties after treatment with metal ions, organic solvents, and surfactants, namely Mg 2+ , methanol, and Triton-100X. Besides, using recombinant lipase lipAB as catalysts, biodiesel was synthesized using rapeseed oil under 50 °C for 72 h with a yield of 90.23%. Thus, the current study confirmed that co-expression of lipase and its chaperone is an effective strategy to enhance enzyme activity and improve the biochemical profile, meanwhile, showing that lipAB is a promising biocatalyst for biodiesel production.
研究领域:
生物技术
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生物化学
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可再生能源
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 23
·12518
·
IF 4.9
Mingyu Jia,Jueyu Wang,Yuxuan Liu,Daizong Cui,Min Zhao
In this study, Fe3(PO4)2·8H2O magnetic nanoparticles (MNPs) were successfully extracted from the strainBurkholderia cepaciaCG-1. We subsequently characterized their composition, structure, and morphology, revealing that these nanoparticles consisted of Fe3(PO4)2·8H2O with an average diameter of 66.87 ± 0.56 nm. Our measurements indicated magnetic parameters of 151 Oe for coercivity, 2 emu/g for saturation remanence, and 16 emu/g for saturation magnetization. Our findings confirmed that these magnetic nanoparticles exhibited intrinsic peroxidase-like activity, catalyzing the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2. Compared to horseradish peroxidase (HRP), the peroxidase mimic demonstrated greater stability under various physicochemical conditions. To investigate the mechanisms underlying the peroxidase-like catalysis of Fe3(PO4)2·8H2O, we employed fluorescence spectroscopy and electron spin resonance (ESR) analysis. The results revealed that the peroxidase-like activity of Fe3(PO4)2·8H2O stemmed from the generation of hydroxyl radicals (·OH). Furthermore, we established a platform for the colorimetric detection of H2O2and glucose. Our method was capable of detecting H2O2concentrations as low as 1.0 × 10−3mmol/L. Impressively, this sensitive method was successfully applied to determine glucose levels in human serum.
研究领域:
生物化学
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纳米医学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
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