Dual-functional C-type fiber SPR biosensor for simultaneous DNA hybridization and temperature sensing
In this work, an integrated temperature compensated surface plasmon resonance (SPR) biosensor based on C-type special fiber is proposed and its application to DNA hybridization is demonstrated. Dual SPR effects were realized on the open microcavity by coating the C-type fiber with a gold film and filling the open microcavity with the temperature-sensitive material polydimethylsiloxane (PDMS). One effect occurs at the gold-analyte interface, where the presence of target DNA molecules interacting with the probe DNA immobilized on the fiber surface modulates the SPR response. The other effect occurs at the PDMS-gold interface, where temperature changes cause the change of PDMS refractive index (RI), altering the SPR response. The proposed sensor demonstrates exceptional capabilities. It can simultaneously detect DNA hybridization and temperature with high sensitivity. Specifically, the sensor exhibits a response of 0.11 nm/nM for DNA molecules and a significant response of −1.97 nm/°C for temperature. Additionally, it boasts a low limit of detection (LOD) of 7.9 nM, making it suitable for applications where early detection is crucial. Beyond its impressive performance, the sensor offers the advantages of compactness and ease of fabrication. This combination makes it a promising candidate for integration into portable devices, enabling on-site and real-time DNA detection with integrated temperature compensation.
危险品化学品经营许可证（带存储）