Marked improvement in the asymmetric reduction of 2-hydroxyacetophenone with mut-AcCR in a biphasic system
( S )-1-Phenyl-1,2-ethanediol (PED) is a vital chiral block in specialty chemical industries. The biotransformation of 2-hydroxyacetophenone (2-HAP) to ( S )-PED was conducted successfully catalyzed with BL21(DE3)(pETDuet- gst -mut- accr - gdh ) which harboring a carbonyl reductase mutant (mut-AcCR). The catalytic activity of the biocatalyst was 15.9 times higher than the recombinant cells harboring original AcCR. 250 mM 2-HAP was reduced, under optimal conditions, and the yield and space-time yield were 95.2 % and 1.89 M/d in the buffer system. Then C 4 MIMI·PF 6 was chosen to be the second phase to improve the catalytic efficiency, and the substrate concentration was increased to 450 mM in the C 4 MIMI·PF 6 /buffer system. After a reaction duration of 3 h, the product yield was over 92 %, and the space-time yield increased to 2.88 M/d which was 1.52 folds higher than that in the buffer system. Also, the product e.e. was over 99 % consistently. Overall, the preparative scale reduction of 2-HAP (450 mM) in C 4 MIMI·PF 6 /buffer system was conducted with promising result. The developed effective C 4 MIMI·PF 6 /buffer system of asymmetric reduction catalyzed by BL21(DE3)(pETDuet- gst -mut- accr - gdh ) will promote the preparation of the enantiomeric pureity chiral alcohol at industrial scale.
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