Journal of Functional Foods
·
2020
·
IF 3.701
Xing Zhang,Wen Shi,Hui He,Ruge Cao,Tao Hou
Val-Phe-Val-Arg-Asn (VFVRN) has been identified and screened from lipid-lowering chickpea peptides (ChPs) previously. This work explored its lipid-lowering mechanisms by using a high-fat diet C57BL/6J mice model and 3T3-L1 preadipocyte cell model. Administration of VFVRN to hyperlipidemic mice significantly decreased the levels of total cholesterol (TC) and total triglyceride (TG) in liver and serum (P < 0.05), and increased the fecal triglyceride excretions. The expressions of sterol regulatory element-binding protein (SREBP)-2, liver X receptor (LXR)α, peroxisome proliferator-activated receptors (PPAR)α and their related target genes were regulated via AMP-activated protein kinase (p-AMPK) pathway in liver. Compared with model group, the levels of PPARα and PPARγ in 10 mg/kg VFVRN group were up-regulated by 36.96% and 36.49% in adipose tissues, while LXRα and ATP binding cassette G5/8 transporters (ABGC5/8) were lowered by 87.26% and 145% in small intestine respectively. Moreover, VFVRN promoted 3T3-L1 preadipocyte apoptosis by regulating the expressions of BaX, Caspase-3 and Bcl-2.
研究领域:
营养学
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分子生物学
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药理学
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产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Weiwei Wang,Yiou Pan,Hang Zhou,Li Wang,Xi Chen,Ge Song,Jianxue Liu,Aike Li
This study aimed to determine whether mice fed ferulic acid (FA) would alleviate high-fat diet-induced obesity and obesity-related metabolic syndromes. Mice were divided into the following four groups and fed for 6 wk (n = 8): control diet (NC), high-fat diet (HF; 60% energy from fat), NC and HF supplemented with 0.5% (w/w) FA respectively. Mice fed HF-FA diet consumed same amount of energy, but had lower daily weight gain, white adipose tissue weight, body fat accumulation, blood glucose and had suppressed hepatic lipid accumulation and inflammatory cytokines (IL-6 and TNF-α) release (P < 0.05), compared with those in HF-fed mice. In conclusion, our findings indicate that dietary FA supplementation suppress body weight gain, body and hepatic fat accumulation, blood glucose elevation and inflammation in high fat diet-induced obese mice, suggesting that FA could be effective in lowering the risk of high fat-diet induced obesity and obesity-related metabolic syndromes.
研究领域:
营养学
·
药理学
·
代谢
✓ 已验证
产品关联
DOI
高影响因子
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
·
2020
·
IF 2.506
Xingchen Liu,Yihan Liu,Qi Cao,Ying Wu,Xueyun Huo,Xiaoyan Du,Zhenwen Chen
Age is an important factor in the evaluation of chemical toxicology. Chemical carcinogenic compounds can induce genomic mutations. However, few studies have been conducted on the association between genomic mutation frequency, such as microsatellite instability (MSI), and the age of mice treated with a nitrosourea mutagen. In the current work, we treated young (6 weeks) and old (10 months) mice with N -methyl- N -nitrosourea (MNU) for 4 months; the MSI frequency was then measured using polymerase chain reaction (PCR) and short tandem repeat (STR) scanning. The percentage of animals with MSI in the old group was significantly higher than that in the young group (100% and 75%). The frequency of MSI events was significantly different between the two groups as well (15.8% for old and 9.4% for young). The ratio of MSI loci displayed no obvious difference between the two groups. In addition, a few loci, including D15Mit5 and D8Mit14 exhibited the highest frequency of MSI events. Since specific loci showed increased MSI in the present study and a higher frequency in previous studies, these loci could be regarded as “hot spot”. These results suggested that old mice would be more susceptible to this mutagen, and prone to accrue MSI. The hot spot microsatellite loci are potentially useful markers for genomic instability analysis.
研究领域:
毒理学
·
遗传学
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DOI
PubMed
高影响因子
KIDNEY & BLOOD PRESSURE RESEARCH
·
2023
·Vol. 48
·期 1
·405-413
·
IF 2.8
Peng Zining,Wang Xueyi,Zhu Qing,Wang Huili,Li Bing,Pang Xinxin,Han Jiarui
Introduction: Diabetic nephropathy (DN) is a common complication in diabetic patients. Chemerin, a novel adipokine, has been associated with renal damage in DN. The chemerin chemokine-like receptor 1 (CMKLR1) has been reported to participate in DN. In this study, we aimed to investigate the effect of a CMKLR1 antagonist, 2-(anaphthoyl)ethyltrimethylammonium iodide (α-NETA), on DN. Methods: To induce diabetes, 8-week-old male C57BL/6J mice were given a single intraperitoneal injection of 65 mg/kg streptozotocin (STZ). Diabetic mice were randomly assigned to receive daily doses of 0, 5, or 10 mg/kg α-NETA for 4 weeks. Results: α-NETA dose-dependently induced body weight and reduced fasting blood glucose levels in STZ-induced diabetic mice. Furthermore, α-NETA significantly reduced the expressions of renal injury markers, including serum creatinine, kidney weight/body weight, urine volume, total proteins, and albumin in the urine, and increased creatinine clearance. Periodic acid-Schiff staining also indicated that α-NETA could effectively ameliorate renal injuries in DN mice. In addition, α-NETA inhibited renal inflammation and the expressions of chemerin and CMKLR1 in mice with DN. Conclusion: In summary, our findings suggested that α-NETA has beneficial effects on the management of DN. Specifically, α-NETA effectively ameliorated renal damage and inflammation in a dose-dependent manner in mice with DN. Thus, targeting the chemerin and CMKLR1 axis with α-NETA may be a promising therapeutic strategy for the treatment of DN
研究领域:
药理学
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肾病学
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糖尿病研究
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DOI
PubMed
高影响因子
JOURNAL OF COMPARATIVE NEUROLOGY
·
2022
·Vol. 531
·期 2
·294-313
·
IF 3.028
Xulin Li,Gaoyang Zhao,Hongren Huang,Jialin Ye,Junfeng Xu,Yuan Zhou,Xiaojuan Zhu,Liping Wang,Feng Wang
Many brain functions that underlie behavior, cognition, and emotions vary with age, as does susceptibility to neuropsychological disorders. The expression of specific genes that are involved in these functions, such as the genes encoding for oxytocin, its receptors, and apolipoprotein D, varies with age across different brain regions. The cannabinoid 1 receptor (CB 1 R) is one of the most widely spread G-protein coupled receptors in the central nervous system and is increasingly recognized for its important contribution to various brain functions. Although changes in CB 1 R expression with age have been reported in the male mouse brain, they have not been well investigated in the female brain. Here, we used fluorescence in situ hybridization to target CB 1 R mRNA in the whole brains of female C57BL/6J mice aged 4, 6, 12, 52 (12 months) and 86 weeks (20 months), and quantified CB 1 R-positive cells in 36 brain regions across the whole brain. The results showed that CB 1 R-positive cells number changed with age. Specifically, CB 1 R expression increased with age in some subregions of the cortex, decreased with age in the lateral septal area, and reached its lowest level at 52 weeks in the thalamus, hypothalamus, and hindbrain subregions. Cluster analysis revealed that some brain regions shared similar temporal characteristics in CB 1 R-positive cell number across the lifespan. Our results provide evidence that investigation of the neural basis of age-related characteristics of female brain functions is not only warranted but required.
研究领域:
神经科学
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分子生物学
·
衰老
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Yuxiang Gu,Yajie Niu,Yanbo Wang,Baoguo Sun,Shuxing Chen,Lishui Chen,Hao Dong,Yuyu Zhang
This study investigated the DPP-IV inhibitory activity of taste-modulating peptides from porcine bone and elucidated their inhibitory mechanisms. Sensory evaluation identified seven peptides with effects on umami taste or saltiness enhancement. Computational tools predicted that they all possessed DPP-IV inhibitory potential. Post-digestion, GPTAANRM, GPGCKAGL, NLRDNYRF and GVNAMLRK exhibited superior DPP-IV inhibition (IC 50 : 206.16 - 317.59 μmol/L). Molecular docking revealed that all these four peptides interacted with active residues in S1, S2 and S3 pocket of DPP-IV and the Lineweaver-Burk plots indicated their competitive inhibition mechanism. All these peptides alleviated insulin resistance in HepG2 cells to some extent, with GPTAANRM and GPGCKAGL notably reducing the blood glucose curve area in C57BL/6J mice. This research enhances our understanding of the beneficial role of taste-modulating peptides in diabetes management and suggests their potential in developing dietary strategies to optimize glycemic control in diabetic individuals.
研究领域:
生物化学
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食品科学
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药理学
·
分子生物学
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营养学
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糖尿病研究
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产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Yong-Yu Yang,Jing-Jing Qi,Si-Yi Jiang,Ling Ye
Ethnopharmacological relevance Cortex fraxini (also known as qinpi)—the bark of Fraxinus rhynchophylla Hance (Oleaceae)—is widely used as a Chinese traditional medicinal for its anti-inflammatory and anti-hyperuricemic activities. Aim of the study Obesity-induced insulin resistance (IR) is driving the rising incidence of type 2 diabetes mellitus and is related to pathological adipose tissue remodeling. Esculin, a major active component of Cortex fraxini , has anti-diabetic effects. However, whether esculin improves obesity-induced IR by regulating adipose tissue remodeling is unclear. The aims of the present study were to assess the effects of esculin on obesity-induced IR and to explore the underlying mechanisms. Materials and methods Obese IR C57BL/6J mice were treated with esculin (40 or 80 mg/kg/day) for 4 weeks. Oral glucose tolerance tests were used to assess insulin sensitivity. Histological analyses were performed to analyze the number and size distribution of adipocytes. Glucose uptake was assessed using 2-NBDG. Results Esculin had no effect on body weight gain but reduced fasting blood glucose, improved oral glucose tolerance, and increased insulin sensitivity. Esculin reduced adipocyte size and the expression levels of collagen 4A1 and tumor necrosis factor α and increased the number of adipocytes and the expression of vascular endothelial growth factor A. Esculin promoted the differentiation of 3T3-L1 cells and upregulated the mRNA expression of CCAAT/enhancer-binding protein α and peroxisome proliferator-activated receptor-γ, activated the insulin receptor substrate 1 (IRS1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway, and enhanced the translocation of glucose transporter type 4 (GLUT4) and glucose uptake in adipocytes treated with palmitic acid. Conclusions These data suggest that esculin increases insulin sensitivity by improving adipose tissue remodeling and activating the IRS1/PI3K/AKT/GLUT4 pathway.
研究领域:
药理学
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内分泌学
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代谢
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中药
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2024
·
IF 4.4
Xuening Ma,Qianchao Shao,Shuxian Huang,Weigao Zhang,Hu Liu,Xu Jiayi,Xunan Zhao,Peiqi Li,Da Shao,YuanQing Bu,Dan Weng
The increasing incidence of cancer underscore the necessity of investigating contributors such as endocrine-disrupting chemicals (EDCs), including bisphenol A (BPA). Although BPA's risks are well-documented, comprehensive studies on its substitutes, such as bisphenol B (BPB), are limited. Dysregulated lipid metabolism is a hallmark of cancer progression. Our previous work demonstrated that BPA and bisphenol S (BPS) disrupt lipid metabolism via the peroxisome proliferator-activated receptor γ (PPARγ) pathway. We hence hypothesized that BPB might similarly perturb lipid metabolism and promote tumor growth. BPB's impact on lipid metabolism was investigated in vitro and in vivo using B16 melanoma cancer cells. Our findings indicate BPB exposure significantly increased lipid metabolism in B16 cells, enhancing cell proliferation and migration, and promoting tumor development in mice. Utilizing siRNA transfection or chemical inhibitor, we found that stearoyl-CoA desaturase-1 (SCD1), a key enzyme in lipid synthesis pathway, was required for BPB-induced lipid accumulation and cancer cell migration. Docking analysis revealed BPB may activate gene expression related to lipid metabolism and angiogenesis by interacting with PPARγ and hypoxia-inducible factor-1α (HIF-1α). This study illuminates BPB's potential role in advancing melanoma through lipid metabolism manipulation, highlighting the need for further research into the safety of BPA substitutes and their impact on cancer development.
研究领域:
肿瘤学
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分子生物学
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环境健康
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 2
·722
·
IF 4.6
Jinyuan Shi,Qianqian Wang,Chuang Li,Mengyu Yang,Muhammad Hussain,Junhui Zhang,Fengqin Feng,Hao Zhong
The roles of medium- and long-chain triacylglycerols (MLCT) on health benefits under high fat diet (HFD) conditions remain in dispute. This study was conducted to investigate the effects of novel LaPLa-rich MLCT on the glycolipid metabolism and gut microbiota in HFD-fed mice when pork fat is half replaced with MLCT and palm stearin (PS). The results showed that although MLCT could increase the body weight in the mouse model, it can improve the energy utilization, regulate the glucose and lipid metabolism, and inhibit the occurrence of inflammation. Furthermore, 16S rRNA gene sequencing of gut microbiota indicated that PS and MLCT affected the overall structure of the gut microbiota to a varying extent and specifically changed the abundance of some operational taxonomic units (OTUs). Moreover, several OTUs belonging to the generaDorea, Streptococcus, andg_Eryipelotrichaceaehad a high correlation with obesity and obesity-related metabolic disorders of the host. Therefore, it can be seen that this new MLCT has different properties and functions from the previous traditional MLCT, and it can better combine the advantages of MLCT, lauric acid, and sn-2 palmitate, as well as the advantages of health function and metabolism. In summary, this study explored the effects of LaPLa-enriched lipids on glycolipid metabolism in mice, providing theoretical support for future studies on the efficacy of different types of conjugated lipids, intending to apply them to industrial production and subsequent development of related products.Keywords:MLCT;lipid metabolism;glucose homeostasis;gut microbiota;inflammation
研究领域:
营养学
·
代谢学
·
微生物学
·
动物科学
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DOI
PubMed
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Mengyao Wu,Xiaole Chen,Lei Zhang,Yingbin Shen,Tak-Ho Lo,Xinquan Yang
Termitomyces species are edible mushrooms that have been documented to possess beneficial medicinal properties. Termitomyces intermedius is a recently identified species that has not yet been fully investigated for its potential effects on obesity. This study aimed to evaluate the anti-obesity effects of Termitomyces intermedius methanolic extracts (TIE) in C57BL/6J mice subjected to a high-fat diet (HFD). Over a 12-week period, the mice were treated with TIE at daily doses of 75 and 150 mg/kg. A comprehensive analysis of obesity-related indicators, serum metabolomics, gut microbiota composition, and flavonoid profiling was conducted. Our results indicated that high dosage of TIE intervention significantly prevented weight gain by nearly 60% when compared to control group, along with improved glucose homeostasis, lowered circulating triglycerides, cholesterols, low-density lipoprotein-cholesterols, alanine aminotransferase, aspartate aminotransferase levels and increased high-density lipoprotein cholesterols. TIE supplementation reduced hypertrophy of hepatocytes and epididymal adipocytes. Metabolomic analysis identified 2,788 metabolites in total and TIE enriched 30 of them, including 7 metabolic pathways associated with lipid and amino acid metabolism. Furthermore, the intervention was associated with an enrichment of beneficial bacterial taxa including Muribaculaceae, Blautia and a reduction in potentially harmful microbes including Alistipes and Bacteroides , suggesting a positive modulation of the gut microbiome. Flavonoid profiling revealed the rich abundance of apigenin, luteolin and quercetin which are believed to contribute to its anti-obesity properties. As the first study to investigate the pharmaceutical properties of Termitomyces intermedius , this work underscores the multifaceted anti-obesity pathways of TIE, including the synergistic impact of its flavonoid constituents. These findings provide a robust theoretical basis for further exploration of TIE's therapeutic potential in personalized nutrition and dietary interventions for obesity management.
研究领域:
药理学
·
营养学
·
微生物学
·
代谢组学
·
民族植物学
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生物医学研究
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Shun Zhong,Yao Yao Qi,Yuan Yuan,Li Lian,Zeyuan Deng,Feng Pan,Junfu Zhou,Zhiyu Wang,Hongyan Li
Background There are few studies about the differences in the composition of moisture, ash, crude protein, crude fat, crude polysaccharide and ergothioneine in Ganoderma lucidum spore powder (GLSP) from different origins. As for GLSP after oil extraction (OE-GLSP), there are still lots of bioactive substance in it. It can be seen that OE-GLSP has certain biological activity. The effect of OE-GLSP on the improvement of intestinal barrier function has been less studied. Results The results showed that there were significant differences for GLSP from five different origins (Anhui, Jilin, Jiangxi, Shandong and Zhejiang) in moisture (0.065–0.113%), ash (0.603–0.955%), crude fat (42.444–44.773%), crude polysaccharide (2.977–4.127%), crude protein (14.761–17.639%) and ergothioneine (0.552–1.816 mg g −1 ) ( P < 0.05). The monosaccharides of GLSP polysaccharide mainly consist of glucose, galactose, mannose, rhamnose, etc. Moreover, the effects of OE-GLSP supplementation on the regulation of organ index, colonic tissue and intestinal microbiota in C57BL/6J mice were investigated. The supplement of OE-GLSP could restore the organ index and weight loss of antibiotic-treated mice. Moreover, OE-GLSP led to the improvement of intestinal dysbiosis by enriching Bacteroidetes, Firmicutes, Lactobacillus and Roseburia , and increasing the Firmicutes/Bacteroidetes ratio. In addition, OE-GLSP intervention repaired intestinal barrier dysfunction by increasing the expression of tight junction proteins (Occludin, Claudin-1 and E-cadherin). Conclusion Different GLSP from five origins exhibited significant differences in microstructure and contents of crude polysaccharide, crude protein, crude fat, water, ash and ergothioneine. Moreover, it was found that OE-GLSP could improve the intestinal barrier function and induce potentially beneficial changes in intestinal flora. © 2024 Society of Chemical Industry.
研究领域:
药理学
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微生物学
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Weiqi Xu,Yujie Huang,Sainan Tao,Wangting Zhou,Yujia Peng,Wei Dong,Xuhui Kan,Guijie Chen,Xiaoxiong Zeng,Zhonghua Liu
The effects of theasinensin A (TSA) on healthy C57BL/6J mice were investigated. Results showed that long-term administration of 100 mg/kg body weight/day TSA might be non-toxic to healthy mice based on the unaltered basal biochemical indicators related to glucose and lipid metabolism, inflammatory factors and hepatic injury. On the contrary, TSA stimulated the rate of lipid turnover and browning of white adipose tissues, accelerated the adipocytic energy mobilization, and then reduced the white adipocytic size, ultimately enhancing resistance to obesity in healthy mice. Furthermore, TSA not only up-regulated the expression of mucin, tight junction protein, and short-chain fatty acids receptor, but also regulated the intestinal microbiota by enhancing the typical beneficial microbe Akkermansia muciniphila , thereby modulating the colonic microenvironment. These results suggested that TSA had a potential strengthening effect on the resistance of healthy mice to metabolic disorders, which provides a theoretical basis for the utilization of TSA.
研究领域:
药理学
·
营养学
·
微生物学
·
代谢学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）