ENVIRONMENTAL AND EXPERIMENTAL BOTANY
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2025
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IF 4.7
Qi Liang Zhu,Nian Zhao Wang,Wen Hao Han,Shu Qi Ma,You Lv,Yan Ping Wang
Root-microbe transboundary communication is important to dominate the interaction between plant and microorganism. However, the influence of quorum sensing signals among bacteria community on the root growth and development is still obscure. The study focused on one quorum sensing signal isolated from poplar rhizosphere, N -octanoyl-DL-homoserine lactone (C8-HSL), and examined its influence on the morphological structure, ultrastructure, and subcellular structure of poplar roots. Transcriptome and metabolome sequencing were further performed to reveal the potential mechanisms of C8-HSL regulating the root morphological development. The results showed C8-HSL demonstrated dual regulatory effects on the growth of poplar fine roots. At high concentrations (more than 100 μM), root growth of poplar seedlings was suppressed by C8-HSL, and the primary and lateral roots appeared short and thick. Conversely, at low concentrations (10 nM), root growth was promoted by C8-HSL, and the primary and lateral roots were elongated. Fluorescent probes indicated that Ca 2 + , reactive oxygen species (ROS), and nitric oxide (NO) in root tip cells were significantly up-regulated at concentrations more than 1 μM of C8-HSL, while only Ca 2+ and NO concentration were significantly up-regulated at concentrations less than 1 μM of C8-HSL. Transcriptome and metabolome analysis further revealed that high concentration C8-HSL significantly upregulated the expression of poplar genes related to secondary wall biogenesis (e.g., MYB46 , MYB83 , CESA ) and auxin transport (e.g., PIN6 , AUX22B , GH3.6 ). In contrast, low concentration C8-HSL upregulated calmodulin expression and increased indoleacrylic acid and 3-indolebutyric acid content. Taken together, these findings provide a deeper insight to the interaction between root and microbes, and imply the potential utilization of quorum sensing signals in regulating root growth.
研究领域:
植物生物学
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微生物学
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分子生物学
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生物信息学
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信号转导
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Shumin Qin,Bixiang Nie,Fu Wei,Yan Lin,Lang Jiang,Qian Zhang
The symbiotic system of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria and Chlorella shows significant nitrogen and phosphorus removal in biogas slurry treatment. It uses quorum sensing signaling molecules to form symbiotic biofilms, significantly boosting nitrogen and phosphorus removal efficiency. This study systematically explored the effect of exogenous N-octanoyl-DL-homoserine lactone (C8-HSL) concentrations on pollutant removal efficiency, extracellular polymeric substance (EPS) production, and microbial dynamics in the HN-AD bacteria- Chlorella symbiotic system. Results showed that the pollutant removal of the symbiotic system is maximized when the C8-HSL concentration is 0.8 μM. At concentrations below 0.8 μM, the system significantly enhances pollutant removal through increased EPS secretion. However, when the concentration exceeds 0.8 μM, both EPS production and pollutant removal are inhibited. Metagenomic analysis shows that C8-HSL at 0.8 μM can enrich key bacterial genera such as Halomonas and Sphingobium . By regulating key genes like narGHI , nirKS , phnX , and ppa , it adjusts their metabolic pathways, thereby influencing nitrogen and phosphorus removal. This highlights the crucial role of C8-HSL concentration in pollutant removal within HN-AD- Chlorella symbiotic systems.
研究领域:
环境微生物学
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水处理技术
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生物修复
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分子生态学
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环境工程
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微藻生物技术
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信号分子调控
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产品关联
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2025
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IF 8.4
Shumin Qin,Lin Deng,Yan Lin,Lang Jiang,Xilong Liu,Qian Zhang
To enhance the stability of membrane aerated biofilm reactor (MABR) and accelerate the formation of biofilm, this study introduced a symbiotic system composed of heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria and Chlorella into the MABR, and compared it with a control system inoculated with HN-AD bacteria and activated sludge. The effects of adding 0.8 μM exogenous C8-HSL on biofilm formation and the efficiency of nitrogen and phosphorus removal were investigated by comparing systems with and without the signaling molecule. Results showed that 0.8 μM C8-HSL enhanced the removal efficiencies of ammonia nitrogen, total nitrogen (TN), and total phosphorus (TP) by 25.17 %, 36.22 %, and 38.74 %, respectively, and increased extracellular polymeric substances (EPS) production by 68.62 %. High-throughput sequencing identified Desulfomicrobium and Aliidiomarina as the dominant microorganisms involved in nitrogen and phosphorus removal. The nirB gene played a key role in the denitrification process, and phoBR gene was essential for phosphorus removal. This study provides theoretical support for the application of quorum sensing in MABR systems, offering a novel strategy to improve the efficiency and stability of high ammonia wastewater treatment.
研究领域:
环境工程
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微生物学
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生物技术
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废水处理
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Lingjie Liu,Shaoqing Mo,Shaopo Wang,Jing Chang,Jianhui Zhao,Jingjie Yu
Low temperatures were a critical factor that inhibited anaerobic ammonium oxidizing bacteria (AnAOB) activity. This study demonstrated the effect of exogenous zero-valent iron (ZVI) on Anammox-hydroxyapatite (HAP) system at 17°C. The results showed that long-term addition of ZVI increased the system nitrogen removal efficiency by 8.59% and alleviated the inhibition of AnAOB by low temperature. After addition of ZVI, the activity of AnAOB and the heme c content increased by 58.20% and 4.83%, respectively. Moreover, the functional enzyme activities of NIR, HZS, and HZO increased by 94.12%, 36.36%, and 16.67%, respectively. The concentrations of quorum sensing molecules, i.e., C 6 -HSL and C 8 -HSL, increased by 191.17% and 41.54%, respectively, after ZVI addition. Metagenomic analysis revealed that the addition of 5 g/L ZVI stimulated the relative abundance relative abundance of Fe(II) metabolism-related genes in AnAOB at 17 °C, thereby contributing to the uptake, transport, utilization, and storage of Fe(II). The addition of ZVI could improve the nitrogen removal performance of the anammox-HAP system at the low temperature.
研究领域:
环境工程
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
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