MICROCHIMICA ACTA
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2021
·Vol. 188
·期 2
·1-9
·
IF 5.833
Li Wenjun,Chen Siyu,Yang Yue,Song Yiju,Ma Chaoyun,Qiao Xiuwen,Hong Chenglin
A GOx/HRP@ZIF-90 nanomaterial is proposed by coating GOx and HRP in ZIF-90 using a bio-simulated mineralization method to improve the tolerance of the enzyme to the external environment. In the detection process, the ZIF-90 is turned on under mild conditions by the competitive reaction of ATP with Zn 2+ and imidazole-2-carboxaldehyde (2-ICA), and the electrical signal of the system is amplified by the enzyme cascade reaction of GOx and HRP. Finally, based on the signal amplification strategy of the competitive reaction between Zn 2+ and ATP to construct a “signal on” mode, electrochemical immunosensor of GOx-HRP enzyme–linked cascade reaction was prepared. The proposed electrochemical immunosensor shows an excellent analytical performance when detecting CA-125, with good selectivity and stability, with a detection range of 0.1 pg mL −1 –40 ng mL −1 and a detection limit of 0.05 pg mL −1 . The test has been performed using chronoamperometry under a constant voltage of −0.4 V. The immunosensor also shows an excellent performance when analysing human blood samples. The recovery of the immunosensor is 97.94–101.8%, with a relative standard deviation of 3.7–6.1%. The proposed sensor provides a novel idea for clinical use of GOx and HRP enzymes and a new method for the clinical detection of tumor markers. Graphical abstract
研究领域:
生物医学工程
·
分析化学
·
肿瘤标志物检测
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2026
·
IF 3
Jiajia Wang,Chun Kan,Demeng Qian,Mingping Gao,Ayang Zhou,Bao-Kang Jin
CA-125, as a tumor marker for ovarian cancer, plays an importance role for rapid and sensitive detection in early diagnosis. Herein, a label-free electrochemical immunosensor based on COF/PTh/MB nanocomposite was constructed for the detection of CA-125.The TAPB-DMTP-COF material, with its large surface area and stable porous framework, was used as the substrate. However, its poor conductivity hindered electron transfer. To address this, conductive polymer PTh was combined with the COF to enhance the composite's conductivity. Meanwhile, MB was used as a signal probe, and differential pulse voltammetry (DPV) was adopted to measure the electrochemical signal. Under optimized experimental conditions, the proposed immunosensor exhibited excellent performance for the CA-125 detection, with a detection range of 1 mU/mL-10000 mU/mL, and a low detection limit of 0.34 mU/mL (S/N = 3), providing a potential platform for clinical application.
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Shuang Yin,Zhanfang Ma
Sensitivity amplification strategies in electrochemical immunoassays are mainly limited by redox signal leaking, degradation of catalytic activity caused by layers of decoration and the large hindrance effect caused by immunoprobes. Herein, we developed an innovative sensitivity amplification strategy based on the self-sacrificial label-assisted electroactivity conversion of a sensing interface, utilizing Fe 3+ -loaded polydopamine (Fe 3+ -PDA) nanoparticles as the self-sacrificial labels, which can be decomposed under acidic conditions and release Fe 3+ . When the assembled sensing interface was immersed in a prussian blue (PB) precursor solution (a mixed solution of 0.1 M KCl, 0.1M HCl and 1 mM K 3 Fe(CN) 6 ), the as-formed sandwich-type structure was destroyed due to the decomposition of Fe 3+ -PDA caused by HCl in PB precursor solution, resulting in the reduce of interface resistance. The released Fe 3+ reacted with the PB precursor solution and triggered the growth of electroactive PB nanoparticles (PB NPs) on the sensing interface. Assisted by self-sacrificial Fe 3+ -PDA, the sensing interface was converted from electrochemically inactive to electroactive with a strong redox signal, high catalytic activity, and decreased interface resistance. The PB NPs can catalyse H 2 O 2 to amplify the redox signal, thus improving sensitivity. The redox signal and catalysts were generated in the final assembly step, which can avoid signal leaking and decreases of catalytic activity caused by layers of decoration. The decomposition of Fe 3+ -PDA can eliminate the large hindrance effect of the immunoprobe. Ultrasensitive quantification of carbohydrate antigen 125 (CA 125) was realized with a detection range from 0.00001 to 1000 U mL −1 and detection limit of 0.25 × 10 −6 U mL −1 .
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Hongji Li,Junqing Qin,Mingji Li,Cuiping Li,Sheng Xu,Lirong Qian,Baohe Yang
A boron-doped graphene (BG)/boron-doped diamond (BDD) composite film was obtained by continuously growing BDD and BG by chemical vapor deposition. In this process, the sp 3 C C bonds in the diamond phase structure are directly converted into sp 2 C C bonds in graphene to combine the two carbon materials, while BG nanosheets are vertically grown on the exposed planes of BDD crystals. These process features impart the vertical BG (VBG)/BDD film a large specific surface area and high electrocatalytic activity, resulting in an electroactive surface area of 2.35 cm 2 . Electrodeposition can be used to load a large number of Au nanoparticles (NPs) on the VBG/BDD electrode. The Au-VBG/BDD electrode was used to construct an immunosensor capable of simultaneously quantifying carcinoembryonic antigen (CEA) and carcinoma antigen 125 (CA 125) at concentrations of 0.5–100 pg mL –1 and 0.5–100 mU mL –1 , respectively, with corresponding detection limits of 0.15 pg mL –1 and 0.09 mU mL –1 . This method is suitable for the practical detection of CEA, CA125, and other tumor markers in human body fluids, allowing early cancer diagnostics and timely treatment.
研究领域:
材料科学
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分析化学
·
生物医学工程
·
癌症诊断
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DOI
高影响因子
危险品化学品经营许可证（带存储）