SENSORS
·
2025
·Vol. 25
·期 11
·3347
·
IF 3.5
Bei Wang,Gengyu Liang,Lingqin Meng,Han Li,Zishuo Song,Yang Xu,Yonghong He,Deling Duan,Qiuxia Shi,Tian Guan,Ya Gong
Simultaneous detection of multiple biomarkers is essential for effective cancer screening. Taking ovarian cancer as an example, the combined detection of CA125 and HE4 has proven to be the most efficient and accurate among multiple biomarker combinations. In this study, we proposed a dual immunosensor based on weak value amplification (WVA) to detect ovarian cancer. By modifying the sensor surface through a self-assembled monolayer technique and utilizing recombinant protein G for antibody enrichment and directional capture, the sensor enables high-precision, simultaneous detection of CA125 and HE4, with detection limits of 5.39 U/mL and 1.79 ng/mL, respectively. Furthermore, the sensor demonstrates excellent specificity, effectively distinguishing target analytes from non-target molecules. This study provides a novel approach for early cancer screening and clinical diagnosis, highlighting the potential of WVA-based immunosensors in ovarian cancer detection.
研究领域:
生物医学工程
·
肿瘤学
·
诊断技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Carbon Letters
·
2023
·Vol. 33
·期 6
·1811-1817
·
IF 4.5
Chen Junnan,Yu Lingmin,Xu Wenzhen,Lin Tao,Jiang Sicong,Jin Caijin
Graphene-based sensors have emerged as significant tools for biosensing applications due to their unique electrical, mechanical, and thermal properties. In this study, we have developed an innovative and sensitive aptasensor based on the surface-modified graphene for the detection of lung cancer biomarker CA125. The sensor leverages the combination of graphene surface and gold nanoparticles (AuNPs) electrodeposition to achieve a high level of sensitivity and selectivity for the biomarker detection. A noticeable decrease in electron transfer resistance was observed upon the AuNPs deposition, demonstrating the enhancement of electrochemical performance. Our experimental findings showed a strong linear relationship between the sensor response and CA125 concentrations, ranging from 0.2 to 15.0 ng/mL, with a detection limit of 0.085 ng/mL. This study presents a novel approach to lung cancer detection, surpassing the traditional methods in terms of invasiveness, cost, and accuracy. The results from this work could pave the way for the development of graphene-based sensors in various other biosensing applications.
研究领域:
生物医学工程
·
癌症检测
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2024
·Vol. 40
·期 6
·1081-1087
·
IF 1.8
Mei Xuqiao,Zeng Zhenhua,Xu Wenxin,Yang Huicong,Zheng Yuanhai,Gao Haimin,Wu Chuncai,Zheng Yanping,Xu Qiaoli,Wang Guowei,Xu Yuhuang,Wu Ayang
CA125 (carbohydrate antigen 125) is an important biomarker of ovarian cancer, so developing effective method for its detection is of great significance. In the present work, a novel sandwich-like electrochemical immunosensor (STEM) of CA125 was constructed by preparing nanoribbon-like Ti 3 C 2 T x MXenes (Ti 3 C 2 T x NR) to immobilize primary antibody (PAb) of CA125 and UIO-66-NH 2 MOFs structure to immobilize second antibody (SAb) and electroactive toluidine blue (Tb) probe. In this designed STEM assay, the as-prepared Ti 3 C 2 T x NR nanohybrid offers the advantages in large surface area and conductivity as carrier, and UIO-66-NH 2 provided an ideal platform to accommodate SAb and a large number of Tb molecules as signal amplifier. In the presence of CA125, the peak currents of Tb from the formed STEM structure increase with the increase of CA125 level. After optimizing the related control conditions, a wide linear range (0.2–150.0 U mL −1 ) and a very low detection limit (0.05 U mL −1 ) of CA125 were achieved. It’s thus expected the developed STEM strategy has important applications for the detection of CA125. Graphical abstract
研究领域:
生物医学工程
·
分析化学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advances in Materials Science and Engineering
·
2021
·
IF 1.726
Yi Shi,Xianhui Gao,Wei Wei,Youguo Chen
Here, we reported a simple method for recognizing CA125 overexpressed serum samples using array-based fluorescent gold nanoclusters (AuNCs). The sensing array was fabricated by the combination of various CA125 aptamer functionalized AuNCs. The fluorescence of different aptamer-capped AuNCs was quenched to a different extent in the presence of CA125. By comprehensive analysis of these fluorescence changes, unique patterns were formed when CA125 was overexpressed in serum samples. This strategy is successfully used for discriminating CA125 overexpressed human serum samples, which have great importance for the diagnosis of ovarian cancer in the early stage.
研究领域:
纳米技术
·
癌症诊断
·
生物传感器
·
分析化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Xiaojing Zhang,Yanying Wang,Hongping Deng,Xiaoxing Xiong,Huijuan Zhang,Tao Liang,Chunya Li
CA125 is the most widely used biomarker for the early detection of ovarian cancer. Luminescence resonance energy transfer (LRET)-based probes show pronounced specificity and simplicity for CA125 detection, but they suffer from strong interference from autofluorescence of biosamples. To resolve this problem, near-infrared (NIR)-excitable upconversion nanoparticles (UCNPs) was employed as energy donor to fabricate CA125 biosensor. Carbon dots (CDs) were utilized as energy acceptor for the overlap between the upconversion luminescence (UCL) spectrum of UCNPs and the absorption spectrum of CDs. Aptamer-modified UCNPs were combined with CDs through π-π stacking interaction, which triggered the LRET process and induce UCL quenching with a high quenching efficiency of up to 90%. In presence of CA125, the formation of CA125-aptamer complex blocked the π-π stacking and recovered UCL. The UCL intensity increased linearly with the logarithm of CA125 concentration in the range from 0.01 to 100 U·mL −1 . A detection limit of 9.0 × 10 −3 U·mL −1 was obtained. This probe is also available for CA125 detection in human serum and may be a useful tool for the early detection of ovarian cancer.
研究领域:
生物医学工程
·
肿瘤学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Lin Wu,Yuhong Sha,Wenrou Li,Sui Wang,Zhiyong Guo,Jun Zhou,Xiurong Su,Xiaohua Jiang
A novel, disposable and label-free electrochemiluminescence (ECL) immunosensor, based on multi-functionalized graphitic carbon nitride (g-C 3 N 4 ) coated on the one-off screen-printed carbon electrodes (SPCEs), for the detection of tumor marker carbohydrate antigen 125 (CA125) was developed. The multi-functionalized g-C 3 N 4 was prepared with amino-coated Fe 3 O 4 nanoparticles and CA125 antibody (anti-CA125) chemically bound to the surface of carboxylated g-C 3 N 4 simultaneously. The Fe 3 O 4 nanoparticles ensured the presence of multi-functionalized g-C 3 N 4 in the outer Helmholtz plane (OHP) of the ECL immunosensor and even becoming part of the electrode surface. This assembly promoted the electron transfer between g-C 3 N 4 and the electrode resulting in greatly enhanced ECL intensity. The CA125 was selectively captured by anti-CA125 leading to effective inhibition of the ECL signal. Under optimal conditions, this disposable ECL immunosensor exhibited a wide dynamic linear detection range for CA125 (0.001–5 U/mL) with a lower detection limit of 0.4 mU/mL, providing high sensitivity. In addition, the immunosensor also exhibited high specificity, good reproducibility and long-term stability. All these properties offer great promise for rapid, simple, selective and effective detection of CA125 in clinical applications.
研究领域:
生物医学工程
·
分析化学
·
肿瘤标志物检测
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Guojuan Zhang,Yujie Han,Zhiguang Liu,Lifang Fan,Yujing Guo
A triple-amplified and ratiometric electrochemical aptasensor for CA125 was designed based on hemin–graphene/SH-β-cyclodextrin@PdPt nanoflower (H-Gr/SH-β-CD@PdPtNF) composites and an exonuclease I (Exo I)-assisted strategy. In the nanocomposite, hemin acts as an internal reference signal owing to the reversible heminox/heminred pair. PdPtNFs can significantly improve the electron transfer rate. SH-β-CD can efficiently enrich quercetin probes through host–guest recognition and increase the second indicator signal. In the presence of CA125, due to the specific binding between the aptamer and CA125, the conformational change of dsDNA (designed by the CA125 aptamer and its complementary DNA) results in the release of quercetin embedded in dsDNA. Subsequently, the free quercetin and DNA fragments are enriched on the H-Gr/SH-β-CD@PdPtNF-modified electrode. Thus, an enhanced oxidation peak from quercetin (IQ) and a reduced peak from hemin (Ihemin) can indicate the same biological identification event. In addition, the recycling amplification of CA125 by Exo I can effectively assist the increase of the quercetin signal. The value of IQ/Ihemin is linear with the concentration of CA125 in the range from 6.0 × 10–4 to 1.0 × 103 ng/mL, and the limit of detection is 1.4 × 10–4 ng/mL. The recovery of CA125 in human blood serum samples was from 99.2 to 104.4%. The proposed sensor is sensitive and reliable, which provides an avenue for the development of triple amplification and ratiometric signal strategies for detecting tumor markers in clinical diagnostics.
研究领域:
生物医学工程
·
临床诊断
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Danli Hu,Hongfeng Liang,Xinyang Wang,Fang Luo,Bin Qiu,Zhenyu Lin,Jian Wang
A gold nanoparticle (AuNPs)/gallium nitride (GaN) Schottky junction was fabricated by growing AuNPs in situ on the surface of GaN and then etched by H2O2 to appropriate diameter. The photogenerated electrons of GaN can be captured and transferred by the AuNPs to increase the migration efficiency, and the electron–hole pairs were separated, which results in the enhancement of the photoelectric performance of the system. The Fermi energy level of AuNPs and the charge transfer efficiency of the AuNPs/GaN can be adjusted by controlling the size of the AuNPs. Then the AuNPs/GaN Schottky photoelectrode had been applied to develop a novel photoelectrochemical (PEC) aptasensor for the epithelial ovarian cancer marker-CA125 detection. The DNA aptamer of CA125 was modified on the surface of the AuNPs via Au–S bonds. The aptamer can bind with the target with high selectivity, and the photoelectron transfer process of the system can be blocked by the protein, which results in the decrease of the photocurrent of the system. The ratio of photocurrent before and after incubation with CA125 (I1/I0) has a linear with the concentration of CA125 in the range of 1–100 U/mL with a detection limit of 0.3 U/mL. The standard addition recovery rates were between 86.01% and 90.09%. This method showed good sensitivity, selectivity, and reliability in detecting CA125 in serum.
研究领域:
生物化学
·
纳米技术
·
癌症研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-10
·
IF 5.3
Zou Ziwei,Chen Yi,Sun Rengang,Shi Boya,Jiang Li,Huang Feixiang
A surface plasmon resonance imaging (SPRi) biosensor is presented which facilitates rapid and sensitive detection of the ovarian cancer biomarker carbohydrate antigens 125 (CA125). The CA125 protein was specifically captured and directly recognized by polyadenylic (polyA)-modified DNA aptamer on the surface of a Au chip. The biosensor demonstrated a linear range of direct detection of CA125 based on SPRi from 20 nM to 0.5 nM, with a detection limit of 0.47 nM. Furthermore, Fe 3 O 4 nanoparticles were synthesized in situ on the carboxyl-functionalized two-dimensional (2D) material Ti 3 C 2 to obtain MXC/Fe 3 O 4 nanocomposites. The large surface area of Ti 3 C 2 provides ample binding sites for the in situ generation of Fe 3 O 4 and this facilitates subsequent immobilization of DNA aptamers. The amino-modified DNA aptamer interacted with carboxylate sites activated on the surface of Ti 3 C 2 , and the aptamer was immobilized on the MXC/Fe 3 O 4 nanocomposite surface. Due to the magnetic properties of Fe 3 O 4 nanoparticles within the composite material, CA125 protein in serum can be effectively captured and separated under an external magnetic field. The captured protein forms a sandwich amplification structure with the DNA adapter on the surface of the Au chip, thereby enhancing the detection signal of CA125. The biosensor demonstrates a linear detection range from 0.5 pM to 1000 pM, with a detection limit as low as 81 fM. The unique separation capability of the composite nanomaterials enables the biosensor to detect CA125 protein as low as 1 pM in complex liquid matrices such as serum, achieving exceptional selectivity, accuracy, and sensitivity in detecting the ovarian cancer marker. This capability significantly supports the early diagnosis of ovarian cancer. Graphical abstract
研究领域:
生物传感器技术
·
医学诊断
·
纳米材料科学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF IMMUNOLOGICAL METHODS
·
2022
·
IF 2.287
Ren Qiu,Wendi Mu,Chunyan Wu,Mengdie Wu,Jing Feng,Shengzhong Rong,Hongkun Ma,Dong Chang,Hongzhi Pan
CA125 is a tumor marker which mainly exists in ovarian, the detection for it with high sensitivity is conducive to improve the effectiveness of tumor prevention and control at early state. Multi-layer graphene oxide derivatives from graphene, and has poor conductivity and high stacked properties that limit its further application. Multi-layer reduced graphene oxide frame (MrGOF) was composed of single-layer graphene sheet and exhibited 3D structure with good dispersion, better conductivity and electrochemical properties after multi-layer graphene oxide underwent alkaline peeling and thermally reduction, the modified graphene are easy to load and combine functional groups and metal nanoparticles. Covalent organic frameworks (COFs) presents a stable frame and through covalent bond connection and has porous properties to adsorb biomolecules , which allows the immobilization of antibody molecules by the porosity and improve the sensitivity of the detection in sensing field. Through the adsorption of COFs for antibody and the probe labeled with functional graphene, we constructed a sandwich type immunosensor with the new material COF-LZU1 as the platform to anchor the CA125 first-antibody and MrGOF combined with amino group and loaded with silver nanoparticles (AgNPs) as the probe to detect tumor marker CA125. The linear range of detection was from 0.001 U/mL to 40 U/mL, with the detection limit was calculated to be 0.00023 U/mL (S/N =3). The prepared immunosensor showed a good application ability for real human serum, which can be attributed to the adsorption of COF-LZU1 for the CA125 first-antibody, and ability to deliver electrons and signal amplification of AgNPs anchored on the sheet structure of MrGOF.
研究领域:
生物传感器
·
材料科学
·
分析化学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2025
·
IF 2.4
Wei Zhou,Kun Wang,Lipeng Pei
Early and accurate detection of ovarian cancer is essential for improving patient outcomes, as most cases are diagnosed at an advanced stage. Cancer antigen 125 (CA125) is a clinically established biomarker that plays a critical role in the diagnosis, prognosis, and recurrence monitoring of epithelial ovarian carcinoma. This study presents a dual-channel electrochemical platform employing ZnO@TiO 2 nanotube arrays for the immunoanalysis of CA125 in complex biological matrices. The nanostructured electrode, fabricated through a sequential hydrothermal and annealing process, exhibited vertically aligned tubular morphology with a mean length of ~2.5 µm and shell thickness of ~20 nm. Electrochemical measurements leveraged dopamine and cytosine as reduction and oxidation probes, respectively, enabling orthogonal signal acquisition. Differential pulse voltammetry revealed wide linear response ranges: 0.1–1000 mU∙mL -1 for cytosine (R² = 0.996; LOD = 0.0002 mU∙mL -1 ) and 0.1–100 mU∙mL -1 for dopamine (R² = 0.992; LOD = 0.0025 mU∙mL -1 ). Impedance spectroscopy confirmed systematic resistance increases during antibody immobilization and antigen binding. The sensor demonstrated excellent reproducibility (RSD ≤ 8.9%), signal stability over 30 days (≥89.1% retention), and selectivity against six common interferents with <5% deviation. Application in 0.2% diluted serum showed recoveries from 99.6% to 100.0% and RSDs <2.01%, validating its performance in real matrices. The combination of core–shell architecture, dual-probe mechanism, and surface regeneration capability positions this system as a promising diagnostic platform for clinical biosensing.
研究领域:
临床诊断
·
生物分析化学
·
纳米材料
·
电化学
·
生物传感器
·
癌症标志物
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Wendi Mu,Chunyan Wu,Fangfang Wu,Hongmin Gao,Xinshui Ren,Jing Feng,Meng Miao,Hehua Zhang,Dong Chang,Hongzhi Pan
Cancer antigen 125 (CA125) is pivotal as a tumor marker in early ovarian cancer prevention and diagnosis. In this work, we introduced an ultrasensitive label-free electrochemical immunosensor tailored for CA125 detection, leveraging nanogold-functionalized copper-cobalt oxide nanosheets (CuCo-ONSs@AuNPs) as nanocomposites. For the inaugural application, copper-cobalt oxide nanosheets delivered the requisite DPV electrochemical response for the immunosensors. Their large specific surface area and commendable electrical conductivity amplify electron transfer and enable significant gold nanoparticle loading. Concurrently, AuNPs offer a plethora of active sites, facilitating easy immobilization of biomolecules via the bond between amino groups and AuNPs. We employed scanning electron microscopy, transmission electron microscopy, and x-ray photoelectron spectroscopy to characterize the nanomaterials' surface morphology and elemental composition. The electrochemical sensor response signals were ascertained using differential pulse voltammetry. Under optimal conditions, the immunosensor exhibited a linear detection range from 1×10 −7 U/mL to 1×10 −3 U/mL and a detection limit of 3.9×10 −8 U/mL (S/N=3). The proposed label-free electrochemical immunosensor furnishes a straightforward, dependable, and sensitive approach for CA125 quantification and stands as a promising method for clinical detection of other tumor markers.
研究领域:
医学诊断
·
生物传感
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）