PROTEIN AND PEPTIDE LETTERS
·
2018
·Vol. 25
·期 8
·776-782
·
IF 1.039
Huang Xiaoting,Liu Haiyang,Fang Wei,Lin Yan,Tan Ying
Background: Carbohydrate antigen 15-3 (CA15-3), a specific breast cancer-related biomarker in serum, can provide direct information to monitor a patient's postoperative status and can predict recurrence and metastasis of breast cancer. The conventional Enzyme-Linked Immunosorbent Assay
(ELISA) is an effective approach for CA15-3 detection, but there is a high limit of detection which is more than 1 U mL-1. Therefore, a convenient, highly sensitive and specific CA15-3 detection method is needed for earlier prognoses of breast cancer. Objective: In this work, we aim to
develop a novel sensitive and selective immunofluorescence assay for the highly effective detection of CA15-3. Method: We pre-coat the antibody I onto the solid phase to capture antigen CA15-3. The A10254- labeled antibody II identified by electrophoresis and fluorescence spectra recognized
the antigen CA15-3 and formed the sandwich format. The fluorescence signal of A10254 directly relating to the amount of antigen could be detected and quantified by the microplate reader excited at 440nm. Results: The obtained method for CA15-3 detection showed lower limits of detection
(0.11 and 0.18 U mL-1) in a linear range of 0.25-14 U mL-1 in the buffer and 2% serum, respectively. This assay exhibited a good specificity and reproducibility in 2% serum with lower relative standard deviations in different tested concentrations. Conclusion: The developed immunofluorescence
assay enhanced the performance of the conventional CA15-3 ELISA kit. Moreover, the acceptable accuracy and good reproducibility in diluted serum indicated the assay has great potential for the diagnosis of breast cancer in clinical practice.
研究领域:
癌症诊断
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生物医学工程
·
临床化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yawen Wu,Xiaolong Chen,Xianfeng Wang,Mei Yang,Faliang Xu,Changjun Hou,Danqun Huo
The effective detection of CA15-3 is of great significance for the postoperative monitoring and early diagnosis of breast cancer. Here, a fluorescent biosensor based on the fluorescence resonance energy transfer (FRET) between the prismatic hollow Metal-polydopamine frameworks (PHMPF) and 6-carboxyfluorescein (FAM)-labeled protein aptamer (FAM-Apt) was established to detect CA15-3. Characterization of SEM, TEM, EDS, FT-IR, XRD and XPS confirmed that the synthesized PHMPF was a polyhedral prismatic hollow organometallic frame containing the elements of C, N, O, and Co. PHMPF can quench the fluorescent group modified on the aptamer efficiently. Under optimized conditions, the proposed fluorescent biosensor can detect CA15-3 low to 2.56 × 10 −5 U mL −1 . In addition, the excellent selectivity and spiked experiments indicate the proposed biosensor owns potential applications for the clinical diagnosis of tumor markers in body fluids.
研究领域:
肿瘤标志物检测
·
生物医学工程
·
临床诊断
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Dehao Jia,Luyang lv,Weijia Sun,Tian Tian,Binnan Shi,Nuo Zhang,Lei Yang,Qin Wei
CA15–3 serves as a tumor marker for breast cancer, and its sensitive detection is crucial for monitoring the disease and managing its treatment. In this study, an electrochemiluminescence (ECL) sensor for the detection of CA15–3 was prepared by using zirconium metal-organic frameworks (Zr-TCBPE) as an ECL emitter and Pt and Pb nanoparticles grown on graphene (rGO-PtPd) as substrate materials. The formation of a ligand bond between the 1,1,2,2-tetra(4-carboxylbiphenyl) ethylene (H 4 TCBPE) and Zr restricted the intramolecular motion of H 4 TCBPE, thereby suppressing nonradiative transitions and triggering a strong coordination-induced ECL enhancement effect. Simultaneous low-potential oxidation using N 2 H 4 as a co-reactant further significantly enhanced the ECL intensity. This method exhibited a wide detection range of 0.1 mU/mL to 500 U/mL, with a detection limit of 0.026 mU/mL. It demonstrated high accuracy and satisfactory reproducibility, indicating that the coordination-induced ECL sensing approach was highly promising for the analysis of disease biomarkers.
研究领域:
分析化学
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生物化学
·
材料科学
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纳米技术
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肿瘤学
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Lihua Li,Wenzhi Zhang,Huichao Chen,Zhigang Zhao,Meifang Wang,Jiexia Chen
In this work, a method for the visual and electrochemical detection of the breast cancer marker CA15–3 was developed based on the etching reaction of gold/silver core-shell nanoparticles (Au@Ag NPs) by mercury ions (Hg 2+ ). Two kinds of nanocomplex of silicon dioxide nanospheres-antibodies-mercury ions (SiO 2 NSs-Ab-Hg 2+ ) and Au@Ag NPs-antibodies (Au@Ag NPs-Ab) were first prepared in the visual detection. In the presence of antigen, a sandwich structure of immuno-complex was formed through the specific recognition reaction of antigen and antibody. Then, after the addition of pH 4.6 CPBS, redox reaction occurred between Hg 2+ and silver shell. With the increase of the amount of antigen, the silver shell was etched thinner and thinner, and the color changed obviously. Visual detection of CA15–3 could be realized in the range of 30–300 U/mL. In addition, we adopted the DPV signals generated by Hg 2+ etching of silver shell to realize the electrochemical detection of CA15–3. The detection concentration range was 0.1–200 U/mL with a detection limit of 0.05 U/mL.
研究领域:
医学诊断
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纳米生物学
·
分析化学
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Chen Cui,Naxin Liu,Qiurui Nian,Yuntao Xia,Liuchuang Zhao,Leqian Hu,Hongjuan Bai,Dechen Jiang
Improvement in the sensitivity and accuracy of ECL-based biosensors, to a great extent, depends on the fabrication of high-performance electrochemiluminescence (ECL) sensors. Herein, polyethyleneimine (PEI)-grafted bimetallic ZnNi metal-organic framework (ZnNi-MOF/PEI) nanospheres were synthesized to prepare a L012-based ECL immunosensor for detecting carbohydrate antigen 15-3 (CA15-3). The introduced PEI was utilized not only as a bridging element for L012 luminophore and detection antibody (Ab 2 ) immobilization but also as a co-reactant for generating the self-enhanced ZnNi-MOF/PEI-L012@Ab 2 ECL signal probe. Furthermore, Pt nanoparticle-functionalized CeO 2 nanorods (CeO 2 -Pt) featuring high-efficiency electrocatalytic properties, as a co-reaction accelerator, could catalyze H 2 O 2 to generate abundant reactive oxygen species (ROS), further augmenting the ECL signal of ZnNi-MOF/PEI-L012@Ab 2 . In the presence of CA15-3 antigen, the capture antibody (Ab 1 )-conjugated CeO 2 -Pt complex (CeO 2 -Pt@Ab 1 ) and ZnNi-MOF/PEI-L012@Ab 2 would get closer to each other, with the formation of a "signal-on" sandwich-type ECL immunosensor. The ZnNi-MOF-assisted ECL biosensor achieved a detection limit of down to 5.75✕10 -5 U mL -1 . This work shows distinguished potential for measuring low levels of biomarkers that may help in the clinical diagnosis of breast cancer.
研究领域:
分析化学
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生物化学
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生物医学工程
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-8
·
IF 5.3
Han Lei,Cai Shuanglong,Chen Xiaogeng
The development of an ultrasensitive and precise measurement of a breast cancer biomarker (cancer antigen 15-3; CA15-3) in complex human serum is essential for the early diagnosis of cancer in groups of healthy populations and the treatment of patients. However, currently available testing technologies suffer from insufficient sensitivity toward CA15-3, which severely limits early large-scale screening of breast cancer patients. We report a versatile electrochemical immunoassay method based on atomically cobalt-dispersed nitrogen-doped carbon (Co-NC)–modified disposable screen-printed carbon electrode (SPCE) with alkaline phosphatase (ALP) and its metabolite, ascorbic acid 2-phosphate (AAP), as the electrochemical labeling and redox signaling unit for sensitive detection of low-abundance CA15-3. During electrochemical detection by differential pulse voltammetry (DPV), it was found that the Co-NC-SPCE electrode did not have a current signal response to the AAP substrate; however, it had an extremely favorable response current to ascorbic acid (AA). Based on the above principle, the target CA15-3-triggered immunoassay enriched ALP-catalyzed AAP produces a large amount of AA, resulting in a significant change in the system current signal, thereby realizing the highly sensitive detection of CA15-3. Under the optimal AAP substrate concentration and ALP catalysis time, the Co-NC-SPCE-based electrochemical immunoassay demonstrated a good DPV current for CA15-3 in the assay interval of 1.0 mU/mL to 10,000 mU/mL, with a calculated limit of detection of 0.38 mU/mL. Since Co-NC-SPCE has an excellent DPV current response to AA and employs split-type scheme, the constructed electrochemical immunoassay has the merits of high preciseness and anti-interference, and its clinical diagnostic results are comparable to those of commercial kits. Graphical abstract
研究领域:
分析化学
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生物化学
·
电化学
·
癌症诊断
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 18
·4334
·
IF 4.2
Jun Xing,Hongxin Wang,Fei Yan
Vertically ordered mesoporous silica films (VMSF) are a class of porous materials composed of ultrasmall pores and ultrathin perpendicular nanochannels, which are attractive in the areas of electroanalytical sensors and molecular separation. However, VMSF easily falls off from the carbonaceous electrodes and thereby impacts their broad applications. Herein, carbon nitride nanosheets (CNNS) were served as an adhesive layer for stable growth of VMSF on the glassy carbon electrode (GCE). CNNS bearing plentiful oxygen-containing groups can covalently bind with silanol groups of VMSF, effectively promoting the stability of VMSF on the GCE surface. Benefiting from numerous open nanopores of VMSF, modification of VMSF’s external surface with carbohydrate antigen 15-3 (CA15-3)-specific antibody allows the target-controlled transport of electrochemical probes through the internal silica nanochannels, yielding sensitive quantitative detection of CA15-3 with a broad detection range of 1 mU/mL to 1000 U/mL and a low limit of detection of 0.47 mU/mL. Furthermore, the proposed VMSF/CNNS/GCE immunosensor is capable of highly selective and accurate determination of CA15-3 in spiked serum samples, which offers a simple and effective electrochemical strategy for detection of various practical biomarkers in complicated biological specimens.
研究领域:
材料科学
·
分析化学
·
生物医学工程
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电化学
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Rui Han,Guixiang Wang,Zhenying Xu,Leyao Zhang,Qun Li,Yinfeng Han,Xiliang Luo
An antifouling electrochemical biosensing platform was constructed based on conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) planted with designed peptides. The designed peptides containing doping and antifouling sequences were anchored to an electrode surface , followed by the electrochemical polymerization of PEDOT. The negatively charged doping sequence of the peptide was gradually doped into the PEDOT during the polymerization process, and by controlling the polymerization time, it was able to exactly dope the whole doping sequence into the PEDOT film, leaving the antifouling sequence of the peptide stretched out of the PEDOT surface. Therefore, an excellent conducting and antifouling platform was constructed just like planting a peptide tree in the PEDOT soil. With antibodies immobilized on the peptide, an antifouling electrochemical biosensor for the detection of a typical biomarker CA15-3 was developed. Owing to the unique properties of the conducting polymer PEDOT and the antifouling peptide, the electrochemical biosensor exhibited high sensitivity and long-term stability, and it was capable of detecting CA15-3 in serum of breast cancer patients without suffering from biofouling. The strategy of planting designed antifouling peptides in conducting polymers offered an effective way to develop electrochemical sensors for practical biomarkers assaying in complex biological samples.
研究领域:
生物医学工程
·
材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Shenna Chen,Qian Zhao,Lingyang Zhang,Linqian Wang,Yunlong Zeng,Haowen Huang
In this study, a combined detection assay for determination of cancer antigen 15-3 (CA15-3) and copper level in breast cancer serum was developed by use of gold nanorod (GNR)-based plasmonic sensor. Rapid detection of tumor marker (CA15-3) can be accomplished through a simple transduction of optical signal induced by immune-reaction between CA15-3 and GNR@CA15-3 antibody. On the other hand, the copper-induced change of localized surface plasmon resonance of GNRs results in a direct readout of optical signal. Therefore, practical application for combined determination of CA15-3 and copper levels of human serum samples of breast cancer was performed. The results are close agreement with those given by the hospital or other standard approach, confirming this assay with high accuracy. The combined detection strategy provides potential applications for early diagnosing and monitoring the progress of malignant tumor.
研究领域:
生物医学工程
·
癌症诊断
·
纳米技术
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产品关联
DOI
高影响因子
SENSORS
·
2025
·Vol. 25
·期 24
·7462
·
IF 3.5
Yuanyue Lu,Yong Mei,Yingying Gu,Ye Tao,Yuhan Yang,Jiao Yu,Yang Zhang,Lin Liu,Xin Li
An electrochemical immunosensor was fabricated to identify CA15-3, a biomarker for breast cancer (BC). A composite sensor substrate made of “zeolitic imidazolate framework-8” (ZIF-8) and “reduced graphene oxide” (rGO) was chosen and its conductivity was further improved by the addition of chitosan (CS)-doped gold nanoparticles (AuNPs). The CS@AuNPs are able to conjugate with antibodies via the strong Au-S interaction, which offers multiple active sites for antibody immobilization and enhances the sensor performance. This immunosensor is capable of ultrasensitive detection of CA15-3 by specific antigen–antibody –interactions. In healthy people, normal serum CA15-3 is up to 25 U/mL. Under optimized experimental conditions, the alteration in the signal intensity measured by the sensor was related to the CA15-3 activity. The quantitative relationship was linear over 0.001–400 U/mL with a limit of detection (LOD) of 0.0031 U/mL at a “signal-to-noise ratio” (S/N) of 3 and a “correlation coefficient” (r2) of 0.9983. The developed immunosensor showed great accuracy, stability, and selectivity, and was able to detect CA15-3 in human serum samples. These results validate its potential as a reliable analytical platform for BC diagnosis and early clinical screening.
研究领域:
生物传感器
·
肿瘤学
·
电化学
·
纳米材料
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Xiaofang Liu,Guangli Luo,Fan Shi,Qun Wang,Fei Deng,Jingzhou Hou,Danqun Huo,Changjun Hou
DNAzyme-driven DNA walker combined with bimetallic nanozyme for synergistic signal amplification in an electrochemical biosensor. • Enzyme-free and Zn 2+ -dependent operation, which simplifies the assay workflow and enhances robustness. • Quantitative release of nanozymes triggered by target-induced DNA cleavage, enabling highly sensitive and specific detection. Early and accurate detection of breast cancer is essential for improving patient prognosis. Carbohydrate antigen 15-3 (CA15-3) is a key serum biomarker used in clinical monitoring, yet its low abundance in early stages requires highly sensitive detection methods. Herein, we report an electrochemical biosensor that synergistically integrates a DNAzyme-driven DNA walker with bimetallic MnAu nanozymes for CA15-3 detection. Target recognition activates the DNAzyme walker, which progressively cleaves substrate strands and releases MnAuNP signal probes from the electrode surface. This process attenuates the nanozyme-catalyzed electrochemical signal for quantitative analysis. The core-shell MnAuNP provides robust oxidase-like activity, while the DNA walker enables efficient enzyme-free signal amplification. This synergistic amplification strategy achieves a wide linear range (0.05 U/mL–200 U/mL) and a low limit of detection (LOD) of 0.0017 U/mL with excellent selectivity. Satisfactory recovery rates (97.58–105.35%) in human serum confirm clinical potential. This work presents a novel strategy for breast cancer biomarker detection by combining DNA walker with nanozyme catalysis amplification, demonstrating promising potential for point-of-care breast cancer diagnostics. Download: Download high-res image (133KB) Download: Download full-size image
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DOI
高影响因子
BIOMACROMOLECULES
·
2026
·
IF 5.9
Zehui Wei,Shuxian Ge,Yukai He,Lina Mu,Shuangxian Yan,Yu Cheng,Tiantian Gao,Kefan Xie,Miaomiao Zhang,Suhong Wang,Mei Tian
Carbohydrate antigen 15-3 (CA15-3) is an important biomarker for early breast cancer diagnosis and therapeutic monitoring. This work presents an ultrasensitive fluorescent probe based on a dual-ligand peptides/UiO-66-NH2@AYG composite for CA15-3 detection. Dual-ligand peptides (GTTFSNYW and MHYLEYPF) target distinct CA15-3 epitopes, enhancing the binding affinity and specificity. The peptides are immobilized on UiO-66-NH2, whose high surface area supports efficient loading of the fluorescent reporter Acridine Yellow G (AYG). This probe demonstrates exceptional performance with a linear detection range of 0.05–1.5 pg/mL (R2 = 0.9981) and an ultralow detection limit of 0.004147 pg/mL. Practical validation in spiked mouse serum shows recovery rates of 91.57–103.7%, while clinical testing with serum samples from 10 cancer patients reveals a strong correlation with ELISA results (r = 0.9944, P < 0.001). This robust platform offers significant potential for cost-effective, early cancer screening in primary healthcare settings.
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DOI
PubMed
高影响因子
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