ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Xudong Jing,Xinyue Zhang,Jing Huang,Yuquan Gao,Huannuo Tao,Jiayu Yuan,Zhongxue Lin,Jiao Yang,Danfeng Dong,Tianyi Liu,Biao Kong,Feng Xu,Lei Xie
Rational integration of autonomous motion and multifunctional labels into immunosensors represents a highly effective strategy for markedly enhancing the antigen capture efficiency and improving the detection sensitivity. Here, smart asymmetric porous nanomotors (FPSP) are designed and fabricated through a selective interfacial assembly strategy, demonstrating magnetic, photothermal, porous, and catalytic activity. Upon near-infrared irradiation, the mesoporous dopamine shell on the FPSP establishes a localized thermal gradient that propels the nanomotor through thermophoretic forces. FPSP nanomotor labels overcome the low sensitivity of immunosensors in early stage detection caused by low analyte concentrations and complex sample matrices. The carbohydrate antigen 153 (CA153) for breast cancer is chosen as an example. This motion enhances the collision probability between FPSP-antibody and CA153, thereby increasing the capture efficiency compared to the static label 95.8%. FPSP exhibits superb colorimetric signal brightness, fast motion, high peroxidase-mimicking activity, and magnetic separation. Integration of FPSP into a dual-antibody sandwich immunosensor enabled ultrasensitive CA153 quantification, achieving the detection limit of 0.0021 U mL–1, which is approximately 316.5-fold higher than that of static label-based immunosensors. This novel immunosensor significantly advances biosensing capabilities, offering promising applications in cancer diagnostics, environmental monitoring, and other areas requiring high-sensitivity detection.
研究领域:
纳米技术
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生物传感器
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癌症诊断
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
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2017
·Vol. 9
·期 47
·18711-18722
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IF 7.367
Bo Zhang,Wenjuan Ma,Fuxin Li,Weichen Gao,Qian Zhao,Weipan Peng,Jiafang Piao,Xiaoli Wu,Hanjie Wang,Xiaoqun Gong,Jin Chang
Recently, immunochromatography test strips (ICTS) have been fully developed for point-of-care testing (POCT). However, the intrinsic limitations including non-quantitative detection of colloidal gold ICTS and low sensitivity of fluorescence ICTS (FICTS) significantly restrict their further application in clinical diagnosis. Taking advantages of rapid colorimetric qualitative detection and fluorescence quantitation, we designed a kind of sensitive and dual-mode magnetic FICTS (mFICTS) based on PLGA@Fe3O4 super-paramagnetic nanosphere (SPMN) probes quenching multiplex fluorescer on the test line through sandwich immunoreactions. Owing to the large number of Fe3O4 nanoparticles (about 47) encapsulated in one SPMN, about 2680 Cy5 molecules were quenched by one SPMN on the test line such that to significantly improve the analytical sensitivity as well as the detection of whole blood samples via magnetic separation. Moreover, the aggregation of black SPMN on the test line enabled a quick naked-eye screening in 3 min. For high accuracy breast cancer diagnosis, combined determination of carcinoembryonic antigen (CEA) and carbohydrate antigen (CA153) was performed on one mFICTS with the limits of detection of about 0.06 ng mL−1 and 0.09 U mL−1, respectively. Then, more than 50 clinical serum samples were investigated for high-resolution screening by mFICTS, and the results were coincident with those obtained by electrochemiluminescence immunoassay (ECLIA). Thus, the designed mFICTS is suitable for point-of-care diagnostics.
研究领域:
生物医学工程
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分析化学
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临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2018
·
IF 4.244
Zhihua Zheng,Lei Wu,Lang Li,Shenfei Zong,Zhuyuan Wang,Yiping Cui
Accurate and quantitative analysis of breast cancer biomarkers is a particularly requisite in the early diagnosis of breast cancers, especially in real samples. Here, we develop a surface enhanced Raman scattering (SERS) immunoassay biosensor based on a microfluidic chip for the simultaneous detection of multiple breast cancer biomarkers in real samples. In such an immunoassay, immuno-Ag aggregates are labeled with different Raman reporters to detect multiple breast cancer biomarkers in real samples. Moreover, to better enhance the Raman signals, self-assembled silver nanoparticles are introduced on the bottom of the microfluidic channels to obtain the SERS active immune-substrate. In order to avoid the crosstalk of SERS signals, we designed a spatially separated detection biosensor by using multi-channels in a microfluidic chip. In the experiments, CA153, CA125 and CEA are selected as the models of breast cancer biomarkers, which can be simultaneously detected of in human serum with an excellently specificity and a high sensitivity. Furthermore, the proposed SERS microfluidic biosensor can be applied to detect the biomarkers in real samples using a linear regression method for the statistical analysis. Our detection results are in agreement with those obtained using commercial ELISA kits, which proves the reliability of our SERS-based microfluidic immunoassay.
研究领域:
生物医学工程
·
分析化学
·
癌症研究
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）