MOLECULES
·
2022
·Vol. 27
·期 14
·4578
·
IF 4.927
Zheng Wei,Xiaoping Cai,Weifeng Cui,Junping Zhang
A CA19-9 electrochemical immunosensor was constructed using a hybrid self-assembled membrane modified with a gold electrode and applied to detect real samples. Hybrid self-assembled membranes were selected for electrode modification and used to detect antigens. First, the pretreated working electrodes were placed in a 3-mercaptopropionic acid (MPA)/β-mercaptoethanol (ME) mixture for 24 h for self-assembly. The electrodes were then placed in an EDC/NHS mixture for 1 h. Layer modification was performed by stepwise dropwise addition of CA19-9 antibody, BSA, and antigen. Differential pulse voltammetry was used to characterize this immunosensor preparation process. The assembled electrochemical immunosensor enables linear detection in the concentration range of 0.05–500 U/mL of CA19-9, and the detection limit was calculated as 0.01 U/mL. The results of the specificity measurement test showed that the signal change of the interfering substance was much lower than the response value of the detected antigen, indicating that the sensor has good specificity and strong anti-interference ability. The repeatability test results showed that the relative standard deviations were less than 5%, showing good accuracy and precision. The CA19-9 electrochemical immunosensor was used for the actual sample detection, and the experimental results of the standard serum addition method showed that the RSD values of the test concentrations were all less than 10%. The recoveries were 102.4–115.0%, indicating that the assay has high precision, good accuracy, and high potential application value.Keywords:self-assembled membrane;gold electrode;electrochemical immunosensor;CA19-9;gastric cancer
研究领域:
分析化学
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生物传感器
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
·
2021
·Vol. 36
·期 2
·279-284
·
IF 4.023
Min Jiang,Zili Huang,Xin Zhao,Rui Liu,Yi Lv
Pancreatic cancer is a malignant tumour with high mortality. Surgery after diagnosis at the early stage is the most effective treatment. As the most effective biomarker of pancreatic cancer, CA19-9 has important clinical significance for both early diagnosis and surgical prediction. Herein, a single nanoparticle homogeneous immunoassay based on Au nanoparticle tagging was proposed for CA19-9 determination, with a dynamic range from 6.25 to 400 U mL−1 in intensity mode and 6.25 to 100 U mL−1 in frequency mode. The limits of detection were 0.72 U mL−1 (intensity mode) and 0.84 U mL−1 (frequency mode). Accurate results were obtained without any washing and separation procedure, and the matrix effect was avoided by monitoring both the intensity and frequency signal obtained from single nanoparticles. The method was successfully applied to the serological evaluation of patients. The results were well correlated with those measured by conventional clinical methods, demonstrating a promising foreground in the preoperative assessment of resectability and evaluation of disease recurrence.
研究领域:
肿瘤学
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医学诊断
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纳米技术
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生物化学
✓ 已验证
DOI
高影响因子
Alexandria Engineering Journal
·
2025
·
IF 6.8
Xiaohui Wang,Yu Liu,Bude Su,Jinhua Tan,Jianxin Shen,Qi Chen,Yu Gou,Ying Zhang
This study presents a novel electrochemical biosensor for the detection of CA19–9, a crucial pancreatic cancer biomarker, utilizing gold nanoparticles (AuNPs) biosynthesized from mint extract. The green synthesis approach yielded well-dispersed, spherical AuNPs with a characteristic surface plasmon resonance peak at 520 nm. Modification of glassy carbon electrodes with these AuNPs resulted in a significant enhancement of electrochemical properties, including a 50 % increase in peak current and a 66.7 % reduction in charge transfer resistance. The biosensor exhibited a wide linear range from 0.1 to 100 U/mL with excellent linearity (R² = 0.998) and achieved a remarkably low limit of detection of 0.05 U/mL. The sensor demonstrated high specificity against common interfering substances and maintained stability over 100 cycles with less than 5 % decrease in performance. Compared to traditional ELISA methods, this biosensor offers rapid detection, minimal sample preparation, and superior sensitivity. The integration of biosynthesized AuNPs not only enhanced the sensor's performance but also aligned with sustainable practices in nanomaterial synthesis. This innovative approach paves the way for the development of sensitive, eco-friendly, and cost-effective diagnostic tools for early pancreatic cancer detection and monitoring.
研究领域:
生物传感器技术
·
癌症诊断
·
纳米材料合成
✓ 已验证
产品关联
DOI
高影响因子
Alexandria Engineering Journal
·
2025
·
IF 6.8
Yinan Hu,Tao Liu,Bo Guo,Miao Ni,Yanrong Li
This study presents a novel surface-enhanced Raman spectroscopy (SERS) method for detecting CA19–9 in colorectal adenocarcinoma using 4-thiobenzoic acid (4-TBA) functionalized gold nanoparticles (nanospheres). The optimized SERS probes exhibited a maximum enhancement at pH 7 and 50 μM 4-TBA concentration, with stable signal achieved after 2 hours of incubation. The assay demonstrated a wide dynamic range of 1–100 U/mL, with a lower limit of quantification of 1.0 U/mL. Excellent selectivity was observed against common serum interferents, with normalized SERS intensities within 10 % of CA19–9 alone. Recovery studies in spiked serum samples yielded results ranging from 94.5 % to 105.2 %, indicating minimal matrix effects. Clinical validation using 70 serum samples (50 colorectal adenocarcinoma patients, 20 healthy volunteers) showed strong agreement with a commercial ELISA kit (Pearson's r = 0.987, p < 0.0001). Bland-Altman analysis revealed a mean bias of −0.37 U/mL between SERS and ELISA methods. The SERS-based approach offers advantages including rapid analysis (<1 hour), small sample volume requirements (5–10 μL), and potential for multiplexing. This method shows promise for early detection, disease monitoring, and treatment response evaluation in colorectal adenocarcinoma, with potential applications in both clinical and research settings.
研究领域:
肿瘤学
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医学诊断
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纳米技术
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光谱学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2023
·Vol. 148
·期 23
·5905-5914
·
IF 4.2
Tingting Zhang,Yan Ma,Yuzhong Zhang
In this work, label-free electrochemical immunosensing of the cancer biomarker carbohydrate antigen 19-9 (CA19-9) is reported using [Fe(CN)6]3−/4− as a signal probe and a copper organic framework (Cu-BTC) nanocomposite for the amplification of the signal. The immunosensor was fabricated by the following process. First, the Cu-BTC nanomaterial with a larger surface area and good biocompatibility was synthesized to improve the dispersion of gold nanoparticles (Au NPs). Then, nitrogen-doped graphene (N-GR) was combined with Cu-BTC to form the nanocomposite. The synthesized Cu-BTC@N-GR@AuNPs@CS nanocomposite was employed to modify the surface of the immunosensor to accelerate the electron transfer rate and improve the immobilization amount of CA19-9 antibodies (Ab). Various techniques, including TEM, SEM and XPS were used to characterize Cu-BTC and nanocomposites. Differential pulse voltammetry (DPV) was used to measure the electrochemical response of the immunosensor in [Fe(CN)6]3−/4−. The signal intensity of the immunosensor was linearly changed upon increasing the concentration of CA19-9 antigen from 10 μU mL−1 to 100 U mL−1, and a detection limit of 4.2 μU mL−1 was achieved. Furthermore, the immunosensor showed good stability, reproducibility and specificity, indicating its potential application in clinical analysis.
研究领域:
生物医学工程
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分析化学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·Vol. 17
·期 1
·84-91
·
IF 2.7
Miaowen Xu,Gaoyang Chen,Yong Huang,Hangyu Song,Zheng Wu,Fengjuan Jiang,Lei Fu,Caili Bi,Xiaowei Cao,Wei Wei
This paper presents functionalized magnetic nanoparticles (Fe3O4@Au MNPs) combined with the surface-enhanced Raman spectroscopy (SERS) technique for sensitive detection of colorectal cancer (CRC) protein biomarker carbohydrate antigen 19-9 (CA19-9). Fe3O4@Au MNPs were constructed by the PEI-mediated seed growth method. Then, the signal molecule 5-5′-dithiobis (succinimidyl-2-nitrobenzoic acid) was used as a bridging agent to link CA19-9 antibody, and the SERS sensor was prepared. Using this sensor, detection of CA19-9 can be realized with only a one step sampling reaction. Fe3O4@Au MNPs combine the advantages of magnetic materials and noble metal nanoparticles, effectively amplifying the signal by creating numerous “hot spots” within the Au particle gaps and enhancing magnetic enrichment. Consequently, this approach lowers the detection limit (LOD) and enhances detection sensitivity. The ratio of characteristic peak intensities I1392/I1069 was selected for calculation, and a linear equation was constructed with a LOD as low as 0.27 U mL−1 by quantitative detections of the standard antigen. Finally, the sensor was used to analyze the clinical serum samples from CRC patients and healthy individuals, and the detection results were consistent with the actual results. This method exhibits notable advantages, including simplicity, high sensitivity, specificity, stability and reproducibility. It is expected to provide a new promising analytical method for clinical CA19-9 immunoassay.
研究领域:
生物医学工程
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肿瘤学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Siyu Wu,Hongyun Ma,Xiaoyan Chen,Wen Zhong,Yingying Gu,Yuqing Miao,Yarui An
Here, a label-free immunosensor was conducted using rGO/Collard-like Bi 2 S 3 @Au nanocomposite system to detect carbohydrate antigen 19-9 (CA19-9) as a diagnostic biomarker for Pancreatic cancer. The unique morphology of Collard-like Bi 2 S 3 increases its specific surface area, which allows it to immobilize more materials and biomolecules on its surface. To realize the covalent bonding of bismuth-based materials with biomolecules, bismuth-based materials were functionalized by Au NPs. Besides, the superior electrochemical activity and large specific surface area of Bi 2 S 3 @Au improved the stability of the sensor, which made it an ideal candidate for regulating the sensing interface in this study. Meanwhile, reduced graphene oxide (rGO) with remarkable electrical conductivity to improve the current response of the sensing interface, thus broadening the linear range of the immunosensor. Based on the above materials, a label free electrochemical immunosensor was constructed for detecting CA19-9. Under optimal conditions, it displays good linear relationship within the range of 0.01 U mL −1 to 100 U mL −1 , the detection limit being 0.0012 U mL −1 . Additionally, the immunosensor prepared in this work is characterized by superior interference immunity, reproducibility and storage stability, and has yielded satisfactory recovery rate in testing the serum samples of patients.
研究领域:
生物传感器
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癌症诊断
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纳米材料
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Shuo Tian,Jiaping Lin,Wei Chen,Dianping Tang,Yang Ou
The development of rapid and sensitive CA19–9 (carbohydrate antigen 19–9) analytical methods for early screening of potential patients, especially in clinical situations, is important for controlling the progression of pancreatic cancer. Herein, a new photoelectrochemical (PEC) immunoassay has been devised for the ultrasensitive detection of CA19–9 using gold nanoparticle-modified ultrathin ZnIn 2 S 4 nanosheets (Au-UZNS) as the photoactive substrate. Notably, the alkaline phosphatase (ALP) labeled on the detection antibody (ALP-dAb) serves as the signal amplifier that catalyzes the production of large amounts of ascorbic acid (AA) after the sandwiched immunoreaction. The generated AA is an outstanding electron donor, thereby improving the intensity of the photoactive substrate. Under the optimized parameters, the Au-UZNS-based PEC immunosensor displays an excellent response to CA19–9 ranging from 0.01 to 500 U mL −1 with a limit of detection (LOD) of 4.7 mU mL −1 . Generally, the CA19–9 levels in the serum of high-risk pancreatic cancer patients range from 120 to 400 U mL −1 , while the levels in normal humans range from 0 to 40 U mL −1 . Therefore, the exceptionally low LOD value of the proposed method is sufficient to meet the threshold for clinical detection and holds significant potential for further application.
研究领域:
分析化学
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生物分析化学
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纳米技术
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生物医学工程
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 5
·056520
·
IF 4.386
Yarui An,Lin Song,Xiaoyan Chen,Chenming Ni,Kuanzheng Mao,Leijing Zhu,Yingying Gu,Yuqing Miao,Bin Song,Hongyun Ma
This work took advantage of 3D biomimetic hydrangea-like bismuth oxychloride (BiOCl) and PtNi nanocubes to fabricate a facile electrochemical immunosensor for the detection of CA19–9. Herein, APTES functionalized BiOCl (APTES-BiOCl) with large specific surface areas can provide eco-friendly microenvironment for biomolecules. The PtNi nanocubes prepared by the classic hydrothermal method have good conductivity, which can facilitate electron transfer highly efficient. Meanwhile, PtNi nanocubes can immobilize the anti-CA19–9 via covalent Pt-N bonds to anchor a great number of biomolecules more firmly. In addition, reduced graphene oxide (rGO) with excellent conductivity was implemented as a matrix, which greatly promoted electron transfer at the sensing interface. Under the optimal conditions, the proposed immunosensor can detect CA19–9 sensitively, from which a linear range of 0.01–150 U·ml–1 was derived accompanied with a low detection limit of 0.0034 U·ml–1. The results illustrated that the designed immunosensor exhibited excellent specificity, good reproducibility, and satisfactory stability. Moreover, it can be applied in the detection of real samples, which indicated that the immunosensor has great potential for early screening and diagnosis of diseases.
研究领域:
材料科学
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电化学
·
生物传感器
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 7
·3044-3065
·
IF 3.094
Qiu Ren,Dai Jianmin,Meng Lingqiang,Gao Hongmin,Wu Mengdie,Qi Feifan,Feng Jing,Pan Hongzhi
Porous carbon sphere materials have a large variety of applications in several fields due to the large surface area, adaptable porosity, and good conductivity they possess. Obtaining a steady carbon sphere using the green synthesis method remains a significant challenge. In this experiment, covalent organic frameworks (COFs) were used as a precursor and Fe 3 O 4 NPs were integrated into the precursor in order to synthesize a porous carbon sphere material using the one-step pyrolysis method. COFs have an ordered porous structure, perpetual porosity, large surface area, and low density and display good environmental tolerance. These properties make them an excellent precursor for synthesizing porous carbon sphere, which maintains good morphology at high temperatures, and it is not involved in the removal of dangerous reagent and small size restrictions during the synthesis process. In addition to the formation of a porous carbon sphere, transition metal carbon material that contains N element can be an active catalyst. The composites exhibit better activity when Fe is doped into carbon materials containing N element than that of other doped transition metals including Mn and Co. In this situation, the integration of Fe 3 O 4 NPs and N element in the COF precursor exposed the active sites of the composites and the two substances synergistically improved the electrocatalytic properties, and the composites were named Fe 3 O 4 @NPCS. The constructed Fe 3 O 4 @NPCS/GCE immunosensor was applied as a means of detecting CA19-9 antigen and presented a wide linear range from 0.00001 to 10 U/mL with a low detection limit of 2.429 μU/mL (S/N = 3). In addition, the prepared immunosensor was utilized for detecting CA19-9 antigen in the real human serum, and the recovery rates were in the range from 95.24% to 106.38%. Therefore, a porous carbon sphere prepared by COFs as a precursor can be applied for the detection of CA19-9 antigen in real samples, which could be an excellent strategy for CA19-9 antigen detection and could potentially promote the development of COF materials in various electrochemical fields.
研究领域:
分析化学
·
材料科学
·
生物传感器
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jianping Li,Xionghui Ma,Mengxi Li,Yun Zhang
A novel glycoprotein imprinting strategy was proposed and was applied to the detection of the carbohydrate antigen 19-9. The glycosylated complex of glycoprotein was used as template to construct a glycosyl imprinted sensor. The eluted imprinted cavities showed good affinity for template glycosyls and glycoproteins carrying template glycosyl. The effect of template saccharide structure on glycosyl imprinted sensors is further discussed. More complex template structures can lead to better sensor performance including selectivity and sensitivity. As a result, the polysaccharide imprinted sensor showed preeminent linear response to CA19-9 in the range of 0.1–5 U/mL, with a detection limit of 0.028 U/mL (3δ/K), while the linear of the monosaccharide imprinted sensor was 1–60 U/mL and the detection limit was 0.17 U/mL. The complex structure on the template surface provides more possibilities for the recognition of the template molecules, consequently, led to the significant anti-interference capability of the polysaccharide imprinted sensor. Furthermore, recoveries ranging from 93.0% to 103.5% were achieved when human serum samples were assayed using the polysaccharide imprinted sensor.
研究领域:
生物化学
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医学诊断
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC CANCER
·
2018
·Vol. 18
·期 1
·1-11
·
IF 3.288
Liu Hai-Ning,Wu Hao,Tseng Yu-Jen,Chen Yan-Jie,Zhang Dan-Ying,Zhu Lin,Dong Ling,Shen Xi-Zhong,Liu Tao-Tao
Background Many novel diagnostic biomarkers have been developed for gastric cancer (GC) recently. We chose two methods with high diagnostic value, the detection of serum microRNAs and metabolomics based on gas chromatography/mass spectrometry (GC/MS), and aimed to establish appropriate models.Methods We reviewed the diagnostic accuracies of all microRNAs identified by previous diagnostic tests. Then appropriate microRNAs and their combinations were validated the diagnostic value. We included 80 patients with GC and 82 healthy controls (HCs) and detected the expression of the microRNAs. GC/MS analysis was conducted, and we used three multivariate statistical analyses to establish diagnostic models. The concentrations of carcinoembryonic antigen (CEA) and carbohydrate antigen 19–9 (CA19–9) were detected for comparison with the novel models.Results Sixty-seven published studies and 70 microRNAs were finally included in the systematic review. MiR-18a, miR-19a, miR-21, miR-92a, miR-199a and miR-421 were chosen to further validate their diagnostic efficiencies. Five of those microRNAs in GC patients had significantly different expression. The combination of miR-19a and miR-92a had the highest area under the curve (AUC) at 0.850 with a sensitivity of 91.3% and a specificity of 61.0%. The GC/MS analysis performed an excellent diagnostic value and the AUC reached 1.0.Conclusion There is a good potential for microRNAs and GC/MS analysis as new diagnostic methods in view of their high diagnostic value compared with traditional biomarkers.
研究领域:
肿瘤学
·
分子生物学
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医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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