Fan Ying,Gao Ruihan,Jiang Yangming,Bian Bing,Tao Zhu,Wei Gang,Xiao Xin
In this work, we report a conceptual strategy for preventing pesticide degradation using cucurbit[n]uril to encapsulate pyroquilon (Pyn). The host–guest inclusion complexes of pyroquilon (Pyn) with CB[7] and CB[8] were prepared and characterized using 1 H NMR spectroscopy, quadrupole-time of flight mass spectrometry (Q-TOF), isothermal titration calorimetry (ITC) and UV absorbance spectrophotometry. The experimental results revealed that the entire Pyn molecule resides within the cavities of CB[7] and CB[8], forming 1:1 inclusion complexes between CB[n] and Pyn. Our study on the release of Pyn demonstrated that CB[n]@Pyn exhibit sustained- and slow-release properties. Moreover, CB[8] can effectively shield UV irradiation and thereby protect the Pyn from photodegradation. Therefore, CB[7]@Pyn and CB[8]@Pyn have great potential to be used as slow-release agents.
Lingxia Qin,Xinxin Ren,Kaiyue Hu,Di Wu,Zhiyong Guo,Sui Wang,Linwen Jiang,Yufang Hu
Graphical An electrochemical biosensor based on the supramolecular host-guest recognition between cucurbit[7]uril (CB[7]) and L -phenylalanine-Cu(II) complex is reported for pyrophosphate (PPi) and alkaline phosphatase (ALP) analysis. We report an electrochemical biosensor based on the supramolecular host-guest recognition between cucurbit[7]uril (CB[7]) and L -phenylalanine-Cu(II) complex for pyrophosphate (PPi) and alkaline phosphatase (ALP) analysis. First, L -Phe-Cu(II) complex is simply synthesized by the complexation of Cu(II) (metal node) with L -Phe (bioorganic ligand), which can be immobilized onto CB[7] modified electrode via host-guest interaction of CB[7] and L -Phe. In this process, the signal of the complex-triggered electro-catalytic reduction of H 2 O 2 can be captured. Next, due to the strong chelation between PPi and Cu(II), a biosensing system of the model “PPi and Cu(II) premixing, then adding L -Phe” was designed and the platform was applied to PPi analysis by hampering the formation of L -Phe-Cu(II) complex. Along with ALP introduction, PPi can be hydrolyzed to orthophosphate (Pi), where abundant Cu(II) ions are released to form L -Phe-Cu(II) complex, which gives rise to the catalytic reaction of complex to H 2 O 2 reduction. The quantitative analysis of H 2 O 2 , PPi and ALP activity was achieved successfully and the detection of limits are 0.067 μM, 0.42 μM and 0.09 mU/mL ( S / N =3), respectively. With its high sensitivity and selectivity, cost-effectiveness, and simplicity, our analytical system has great potential to for use in diagnosis and treatment of ALP-related diseases.
危险品化学品经营许可证（带存储）