PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
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2023
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IF 7.8
Ce Wang,Yuling Lü,Tianxu Ye,Feng Rong,Limin He
This paper demonstrates flotation separation for dispersed oil droplets and dissolved organics by preparing condensate bubble aphrons (CBA) by partially vaporizing condensate. The results show that the new CBA consists of a condensate membrane and a gas core, with a three-phase contact angle above 140° from the oil surface due to excellent wettability . At different temperatures and condensate compositions, bubbles are about 25–100 µm in size. The half-life time (140–230 s) of CBA and gas hold-up (10–25%) is much higher than nitrogen bubbles. It shows that the CBA has good stability and dispersibility . The flotation experiment of CBA found that the condensate group distribution ratio was optimally adapted to the temperature, and the optimum oil removal efficiency of up to 87% was obtained when the operating temperature was higher than the bubble point of 25–35 °C. The experimental experiment shown the recommended initial oil concentration (400 mg/L) and the mixing ratio of condensate and water (1.0%). Otherwise, CBA flotation efficiency was 10% higher than dissolved gas flotation. With the addition of flocculant , the CBA flotation efficiency can reach 97% of oil droplets and can achieve 50% removal of Acetic acid (HAc).
研究领域:
环境科学
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石油工程
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DOI
高影响因子
International Journal of Medical Sciences
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2025
·Vol. 22
·期 4
·775-789
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IF 3.2
Gong Xueqi,Gao Haitong,Wang Wenyuan,Xu Tonghui
Recombinant adeno-associated viral vectors (rAAVs) can effectively deliver transgene to the nervous system. The selection of AAV serotype and promoter significantly influences the dynamics of the transgene expression, including its strength and cell-specificity. Previous studies demonstrated that in neonatal mice, the intramuscular (IM) injection of the rAAV2-retro vector could efficiently deliver transgene to lower motor neurons (LMNs) of the brainstem and spinal cord. However, the best promoter for the expression of transgene in the central neural system (CNS) using rAAV2-retro remains undetermined. This study compared five commonly used promoters, including mouse phosphoglycerate kinase (mPGK), CMV early enhancer/chicken β-actin/short β-globulin intron (CAG), human cytomegalovirus (hCMV), chicken β-actin (CBA), and human synapsin (hSyn) promoters. The IM (unilateral gastrocnemius muscle) injection of rAAV2-retro vectors packaged with the reporter constructs containing each promoter was performed in the newborn C57BL/6J mice. The levels of gene expression and the types of cells were examined using the light-sheet illumination imaging technique and confocal microscopy. Our findings revealed that rAAV2-retro primarily targeted the brainstem and spinal cord within the CNS. Among the five promoters tested, CAG and hCMV showed the highest gene expression. Almost all the transduced cells were identified as LMNs. Additionally, gene expression driven by hCMV was found to be dependent of the inclusion of WPRE and β-globin intron elements. Importantly, none of the promoters induced hepatotoxicity, ensuring the safety of rAAV2-retro-mediated expression. This study provided valuable insights for optimizing the rAAV2-retro-mediated gene delivery system to LMNs in the brainstem and spinal cord, which might have potential implications for research on motor neuron-related diseases.
研究领域:
神经科学
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基因治疗
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分子生物学
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DOI
PubMed
高影响因子
Journal of Immunotoxicology
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2022
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IF 3.439
Wenhua Zhong,Penghuan Chang,Lianfang Gan,Lifan Zhong,Zhaoxin Yang
Most current methods to assess T-cell-dependent antibody responses (TDAR) are semi-quantitative and based on measures of antibody titer generated against a standard antigen like keyhole limpet hemocyanin (KLH). The precision, sensitivity, and convenience of TDAR assays might be improved by applying rapid, sensitive, specific cytometric bead assays (CBA). In the study here, KLH antigen was covalently coupled onto the surface of cytometric beads using immune microsphere technology, and IgM antibody capture spheres were prepared for use in pretreatment processing of samples. The working parameters associated with this novel TDAR-CBA system were optimized in orthogonal experiments. The optimal concentration of the KLH coating solution in this system was 160 μg/ml, that of the anti-KLH IgG capture spheres 6.0 × 105/ml, and the optimal dilution of fluorescein isothiocyanate (FITC)-conjugated Affini-Pure Goat Anti-Mouse IgG (H + L) was 60 μg/ml. Repeated tests indicated that this approach yielded good linearity (r2 = 0.9937) method, with a within-run precision of 3.1–4.9%, and a between-run precision of 4.4–4.9%. This new approach had a limit of detection of 113.43 ng/ml (linear range = 390.63–50 000), and an interference rate of just 0.04–3.51%. Based on these findings, it seems that a new mouse TDAR assay based on CBA can be developed that would appear to be more sensitive, accurate, and precise than the current TDAR assay approaches based on traditional ELISA.
研究领域:
免疫学
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
Materials Today Chemistry
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2024
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IF 6.7
Jinyu Wang,Qianqian Qiao,Yuqi Feng,Yuhao Guo,Tao Liao,Linwei Li,Ying Kuang,Bingbing Jiang,Ziqiang Xu,Cao Li
In cancer treatment, although prodrugs have been developed to overcome obstacles such as undesirable pharmacokinetic performance and serious off-target toxicity of most chemotherapeutics, the combination of prodrugs with nanotechnology could be used to overcome the shortcomings of conventional prodrug strategies and facilitate more efficient drug delivery and accumulation of anticancer prodrugs at specific sites/tissues. Phototherapy is a method that uses near-infrared (NIR) light to achieve local and non-invasive tumor therapy. The combination of chemotherapy and phototherapy has shown many therapeutic advantages, including synergistic therapeutic effects and the reduction of toxic and side effects of drugs through dose reduction. In this work, a hydrazone linkage was reported to conjugate Ti 3 C 2 T x MXene nanosheets with doxorubicin hydrochloride (DOX) and methoxy poly(ethylene glycol) benzaldehyde (mPEG-CHO) through a chemical surface modification to form a two-dimensional (2D) nano-prodrug Ti 3 C 2 T x -DOX/PEG (TDP) for synergistic chemo-photothermal therapy. First, hydrazide functionalized Ti 3 C 2 T x was obtained. Then, the carbonyl group of DOX and the aldehyde group of mPEG-CHO can form a pH-responsive hydrazone bond with hydrazide-functionalized Ti 3 C 2 T x . The high surface area of Ti 3 C 2 T x could overcome the reduced drug loading caused by conjugation. Aqueous stability and biocompatibility were enhanced by the introduction of PEG, making the material capable of prolonged circulation in the body's bloodstream. By achieving excellent antitumor results with this nano-prodrug, we have added another brick to the edifice of Ti 3 C 2 T x MXene as an anticancer drug carrier, and given new vitality to the study of anticancer prodrugs combined with nanotechnology.
研究领域:
肿瘤学
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纳米医学
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药物递送系统
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2017
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IF 4.233
Huan Lv,Xueqin Wang,Qiuxia Fu,Yang Si,Xia Yin,Xiaoran Li,Gang Sun,Jianyong Yu,Bin Ding
Construction ion-exchange membranes with superb biomolecules adsorption and purification performance plays a greatly important role in the fields of biotechnological and biopharmaceutical industry, yet still remains an extremely challenging. Herein, we in situ synthesized the cis-butenedioic anhydride grafted poly(vinyl alcohol) hydrogel nanofibrous membranes (CBA- g -PVA HNFM) by combining electrospinning technique with the grafting-copolymerization crosslinking. Taking advantages of the large specific surface area which could provide numerous sites available for functional groups and biomolecules binding, highly tortuous and interconnected porous channel for biomolecules transfer, and enhanced mechanical strength, the resultant CBA- g -PVA HNFM exhibited relatively high binding amount of 170 mg g −1 , rapid equilibrium time of 8 h towards the biomolecule template of lysozyme, and the performance could be tailored by regulating the buffer properties and protein concentrations. Additionally, the resultant functional HNFM also possessed superior acid resistance property, excellent reversibility and regeneration performance. More importantly, the obtained CBA- g -PVA HNFM could directly extract lysozyme from fresh chicken eggs with capacity of 125 mg g −1 , exhibiting excellent practical application properties. The fabrication of proposed CBA- g -PVA HNFM offers a feasible alternative for construction of ion-exchange chromatograph column for bio-separation and purification engineering.
研究领域:
生物技术
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生物制药
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材料科学
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化学工程
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DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 37
·19981-19995
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IF 11.9
Jinlong Cao,Zhen Zhang,Lin Ye,Xiaowen Zhao
Although electrode materials have been tremendously researched, there are few reports on the design of ordinary and biofriendly polymer electrolytes for developing high-performance actuators. Herein, in view of poly(vinyl alcohol) (PVA) with excellent biocompatibility and low cost, a superior ionic soft actuator based on a PVA-based ionogel electrolyte was successfully developed by constructing continuous ion transport channels. By the introduction of benzoic acid groups into a PVA chain (BPVA), strong hydrogen bonding enriched regions (BPVA-rich phase) formed via their spontaneous self-aggregations. Meanwhile, 1-ethyl-3-methylimidazolium chloride (EMIMCl) had good compatibility with hydroxyl groups on the PVA chain via weak hydrogen bonding to form an EMIMCl-rich phase, resulting in the formation of a bicontinuous phase structure in the ionogel system. Specifically, due to stronger dipole characteristics of the benzoic acid group compared with the hydroxyl group on the PVA chain, the ion–dipole interactions between –CO in benzoic acid groups and EMIM+ formed at the phase interface, which facilitated formation and migration of mobile ions, forming continuous ion transport channels. Besides, strong adhesion between the SWCNT electrode and PVA-based ionogels effectively reduced the interfacial transport resistance of mobile ions. The resulting PVA-based ionogels exhibited exceptional electrical properties with an ionic conductivity of 0.257 mS cm−1 and excellent mechanical stretchability (∼1600% strain). The obtained PVA-based ionic actuator displayed quasi-rectangular CV curves with improved areal capacitances, while the competitive performance of the actuator with a large bending strain of 0.52% at 0.1 Hz, a broad-band frequency response of 0.1–25 Hz, and excellent cycling stability with a bending displacement retention of almost 89% after 3100 cycles in air was exhibited. This work opens up new avenues for developing ionic actuator electrolytes based on biofriendly polymer electrolytes with great potential for artificial intelligence devices.
研究领域:
材料科学
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软体机器人
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生物医学工程
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DOI
高影响因子
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