JOURNAL OF ELECTRONIC MATERIALS
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2024
·Vol. 53
·期 5
·2263-2274
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IF 2.2
Shan Yiting,Yang Chengyu,Lu Yang,Li Junkai,Guo Xiang,Zhou Hongqing
The (1− x )CaO-B 2 O 3 -SiO 2 (CBS)/ x Mg 2 SiO 4 composites were prepared and the influence of CBS crystallizable glass content on the sintering properties, dielectric properties, and thermal properties of the composites were investigated. The primary crystal phases in the CBS/Mg 2 SiO 4 composites were CaMgSi 2 O 6 and CaB 2 O 4 . An appropriate amount of Mg 2 SiO 4 reduced the crystallization temperature of the composite. For x = 0.30, a sintering temperature of 890°C with a holding time of 60 min resulted in a bulk density of 2.645 g/cm 3 , dielectric constant of 6.6, and dielectric loss of 2.54 × 10 −3 at 10 kHz. Prolonged holding time promotes grain growth; when extended from 60 min to 180 min for x = 0.30, the permittivity increased from 6.5 to 6.6 and Q × f increased from 10,100 GHz to 15,700 GHz when x = 0.30 at 13 GHz. The CTE of 70 wt.% CBS + 30 wt.% Mg 2 SiO 4 composite was 9.5 ppm/°C, which approached the value of a circuit board for a ceramic ball grid array package (CBGA).
研究领域:
材料科学
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电子工程
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiuzhen Li,Yuanting Ni,Chenxi Liu,Shaohua Xu,Wei Shu,Hong Liang,Ming Chen
Background Homocysteine (Hcy) has been recognized as an independent risk factor for the development of cardiovascular disease. Many electrochemical detection methods have been developed for analyzing Hcy, however, the specificity of these methods is based on the interaction between the sulfhydryl groups in Hcy and the metallic materials, and thus the specificity of such detection is not satisfactory. Results In this study, an electrochemical sensor based on COFs and Pd@CuO was developed for the specific detection of Hcy. Firstly, COFs material was used as an immobilized carrier for cystathionine β-synthase (CBS), and the immobilized CBS as a recognition unit was able to catalyze the production of H 2 S from the substrates (Hcy and Cys) in a specific manner. Secondly, the Pd@CuO composite material has excellent conductivity and can catalyze H 2 S as a source of electrochemical signal generation. The electrochemical sensor constructed based on the above principles has a good electrochemical response to Hcy within the range of 5 μM to 100 μM, and the limit of detection can reach 0.11 μM (S/N = 3). In addition, the constructed sensor has good reproducibility, stability and specificity, and has good recoveries in the detection of real plasma samples, which provides a potential application for the diagnosis of homocysteinemia. Significance and Novelty The electrochemical sensor significantly increases the specificity of detection by utilizing immobilized Hcy specific catalytic enzymes CBS. In addition, Pd modified CuO@COFs electrode greatly increases the conductivity of the electrode, thereby increasing the sensitivity of detection. These characteristics make the electrochemical sensor have good application prospects for detecting the Hcy in clinic in the future.
研究领域:
生物医学工程
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分析化学
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临床诊断
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高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 31
·6082-6088
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IF 1.915
Gaiyan He,Ruixia Gao,Yi Hao,Xiaoshuang Tang
We have developed a novel flow-injection chemiluminescence analysis method to detect fluorescent whitening agent 2,2′-([1,1′-biphenyl]-4,4′-diyldi-2,1-ethenediyl)bis-benzenesulfonic acid disodium salt (CBS) for the first time. A luminol–urea peroxide chemiluminescence reaction system was adopted, and the instrumental and chemical parameters were respectively optimized. Under optimal conditions, the relationship between the relative chemiluminescence intensity and the concentration of CBS was fitted in linear mode, and satisfactory correlation coefficients were obtained (ΔI = 44.85CCBS + 165.39, CCBS, nmol L−1, r = 0.9978). This assay is linear from 1 to 600 nmol L−1, with limits of detection and quantification of 0.1 nmol L−1 and 0.4 nmol L−1, respectively. And the intra- and inter-day relative standard deviations are less than 1.5% and 4.5%. The proposed flow-injection chemiluminescence method had been successfully used in the determination of CBS in flour, and the recovery is 90.0–104.5%, which shows great potential in control of food safety. This method is sensitive and convenient, and could be comparable with others. Finally, a possible mechanism of chemiluminescence emission was also investigated.
研究领域:
分析化学
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食品科学
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高影响因子
TOXICOLOGY
·
2021
·
IF 4.221
Bobo Yang,Wenjun Zhao,Changsheng Yin,Yu Bai,Suhua Wang,Guangwei Xing,Fang Li,Jinsong Bian,Michael Aschner,Jiyang Cai,Haifeng Shi,Rongzhu Lu
Hydrogen sulfide (H 2 S) as the third gasotransmitter molecule serves various biological regulatory roles in health and disease. Acrylonitrile (AN) is a common occupational toxicant and environmental pollutant, causing brain and liver damage in mammals. The biotransformation of AN is dependent-upon reduced glutathione (GSH), cysteine and other sulfur-containing compounds. However, the effects of AN on the endogenous H 2 S biosynthesis pathway have yet to be determined. Herein, we demonstrated that a single exposure to AN (at 25, 50, or 75 mg/kg for 1, 6 or 24 h) decreased the endogenous H 2 S content and H 2 S-producing capacity in a dose-dependent manner, both in the cerebral cortex and liver of rats in vivo . In addition, the inhibitory effects of AN (1, 2.5, 5, 10 mM for 12 h) on the H 2 S content and/or the expression of H 2 S-producing enzymes were also found both in primary rat astrocytes and rat liver cell line (BRL cells). Impairment in the H 2 S biosynthesis pathway was also assessed in primary rat astrocytes treated with AN. It was found that inhibition of the cystathionine-β-synthase (CBS)/3-mercaptopyruvate sulfurtransferase (3-MPST)-H 2 S pathway with the CBS inhibitor or 3-MPST-targeted siRNA significantly increased the AN-induced (5 mM for 12 h) cytotoxicity in astrocytes. In turn, CBS activation or 3-MPST overexpression as well as exogenous NaHS supplementation significantly attenuated AN-induced cytotoxicity. Taken together, endogenous H 2 S biosynthesis pathway was disrupted in rats acutely exposed to AN, which contributes to acute AN neurotoxicity in primary rat astrocytes.
研究领域:
毒理学
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神经科学
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药理学
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PubMed
高影响因子
ECS Journal of Solid State Science and Technology
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2019
·Vol. 8
·期 10
·N141
·
IF 1.795
Guangyue Shi,Yunpeng Qu,Guohua Fan,Yao Liu
The CNTs-CBs nano-units composed of carbon nanotubes (CNTs) and carbon black (CBs) have been prepared by electrostatic self-assembly. Herein, the CNTs-CBs/PS composites with different CNTs-CBs contents were prepared. The negative permittivity behavior was observed when three-dimensional conductive network was constructed by CNTs-CBs. Tunable and weakly negative permittivity originated from the low frequency plasmonic state which was collective electrons oscillation in CNTs-CBs network. The Jonscher's power law and Drude model were used to analyze the dielectric behavior and conductive mechanism changing. This work has broadened the applications of carbonaceous materials and polymers in the field of metamaterials.
研究领域:
材料科学
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电磁学
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高影响因子
Toxicology Research
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2022
·Vol. 11
·期 2
·374-384
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IF 2.68
Yang Bobo,Yin Changsheng,Zhang Yu,Xing Guangwei,Wang Suhua,Li Fang,Aschner Michael,Lu Rongzhu
BackgroundHydrogen sulfide (H2S), as the third gasotransmitter participates in both cellular physiological and pathological processes, including chemical-induced injuries. We recently reported acute acrylonitrile (AN) treatment inhibited endogenous H2S biosynthesis pathway in rat and astrocyte models. However, there is still no evidence to address the correlation between endogenous H2S and sub-chronic AN exposure.ObjectivesThis study aims to explore the modulatory effects of prolonged AN exposure on endogenous H2S levels and its biosynthetic enzymes in rat blood, brain and liver.MethodsA total of 50 male Sprague-Dawley rats were randomly divided into 5 groups, including the control group and AN-treated groups at dosages of 6.25, 12.5, 25 or 50 mg/kg. Rats received one exposure/day, 5 days/week, for 4 consecutive weeks. The rat bodyweight and brain/liver organ coefficient were detected, along with liver cytochrome P450 2E1(CYP2E1) expression. In addition, the H2S contents in rat serum and plasma, and in cerebral cortex and liver tissues were measured by methylene blue method. The expression of H2S-generating enzymes, including cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MPST) was also measured with Western blot both in rat cerebral cortex and liver.ResultsSubchronic exposure to AN significantly inhibited bodyweight-gain and increased the liver CYP2E1 expression compared with the control. In addition, AN significantly increased H2S levels in rat plasma and serum, but not in liver. The endogenous H2S level in rat cerebral cortex was also significantly increased upon AN treatment, when expression of the major H2S-generating enzymes, CBS and 3-MPST were significantly enhanced. However, hepatic protein levels of CBS and CSE were significantly increased, whereas hepatic levels of 3-MPST were significantly decreased.ConclusionThis study showed that sub-chronic AN exposure increased endogenous H2S contents in rat blood and brain tissues, but not liver, which may be resulted from the distinct expression profile of H2S-producing enzymes in response to AN. The blood H2S contents may be applied as a potential novel biomarker for surveillance of chronically AN-exposed populations.HighlightsSubchronic intraperitoneal exposure to acrylonitrile increased H2S content in rat blood and cerebral cortex, but not in liver.Distinct tissue expression profiles of H2S-producing enzymes contribute to the acrylonitrile-induced differential effects on the H2S level.Blood H2S level may be a biomarker for subchronic exposure to acrylonitrile.
研究领域:
毒理学
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药理学
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生物化学
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PubMed
高影响因子
NEUROENDOCRINOLOGY LETTERS
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2023
·Vol. 44
·期 4
·193-198
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IF 0.7
Cheng X,Ding M,Wei C,Xiao F,Liao J,Li M,Xiao Z,Liu X,Luo Y
Objective Research has shown that hydrogen sulfide (H2S) plays a protective role in many diseases of the nervous system. The aim of this study is to investigate the effect of hypoxia on endogenous H2S concentration in the cerebral cortex of Alzheimer's disease (AD) transgenic mice and its mechanism. Methods AD transgenic mice were raised in closed boxes and pure nitrogen was introduced to reduce the oxygen concentration to 8%-10%, establishing an animal model of hypoxia. Oxygen partial pressure was measured with an oxygen meter. The expression of cystathionine-β-synthase (CBS) in cerebral cortex tissue was determined by Western blot, and H2S concentration was measured by a modified methylene blue method. Results (1) Hypoxia down-regulated CBS expression in cerebral cortex tissue of AD transgenic mice (p < 0.05). (2) The concentration of H2S in the cerebral cortex tissue of the hypoxic transgenic group was significantly lower than that of the Control group (p < 0.01). (3) Overexpression of CBS reversed the hypoxia-induced decrease of H2S concentration in the cerebral cortex tissue of AD transgenic mice (p < 0.01). Conclusions Hypoxia decreased the concentration of endogenous H2S in the cerebral cortex tissue of AD transgenic mice by down-regulating the expression of CBS.
研究领域:
神经科学
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药理学
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分子生物学
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PubMed
高影响因子
Scientific Reports
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2015
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IF 5.578
Ailin Xiao,Hongjuan Wang,Xin Lu,Jianchun Zhu,Di Huang,Tonghui Xu,Jianqiang Guo,Chuanyong Liu,Jingxin Li
H2S is produced mainly by two enzymes:cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), using L-cysteine (L-Cys) as the substrate. In this study, we investigated the role of H2S in gastric accommodation using CBS(+/-) mice, immunohistochemistry, immunoblot, methylene blue assay, intragastric pressure (IGP) recording and electrical field stimulation (EFS). Mouse gastric fundus expressed H2S-generating enzymes (CBS and CSE) and generated detectable amounts of H2S. The H2S donor, NaHS or L-Cys, caused a relaxation in either gastric fundus or body. The gastric compliance was significantly increased in the presence of L-Cys (1 mM). On the contrary, AOAA, an inhibitor for CBS, largely inhibited gastric compliance. Consistently, CBS(+/-) mice shows a lower gastric compliance. However, PAG, a CSE inhibitor, had no effect on gastric compliances. L-Cys enhances the non-adrenergic, non-cholinergic (NANC) relaxation of fundus strips, but AOAA reduces the magnitude of relaxations to EFS. Notably, the expression level of CBS but not CSE protein was elevated after feeding. Consistently, the production of H2S was also increased after feeding in mice gastric fundus. In addition, AOAA largely reduced food intake and body weight in mice. Furthermore, a metabolic aberration of H2S was found in patients with functional dyspepsia (FD). In conclusion, endogenous H2S, a novel gasotransmitter, involves in gastric accommodation.
研究领域:
胃肠病学
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药理学
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生理学
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PubMed
高影响因子
BIOORGANIC CHEMISTRY
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2026
·
IF 5.1
Wenyu Xie,Ke Wu,Lin Zhang,Xiyu Feng,Shangshen Yang,Siyu Jia,Yutong Li,Xiaoming Wang
Hydrogen sulfide (H₂S) plays a crucial neuroprotective role in Parkinson's disease (PD). Cystathionine-β-synthase (CBS), a key enzyme involved in H₂S biosynthesis, exhibits expression deficiencies that are closely linked to PD progression. This suggests that enhancing the CBS–H₂S signaling axis to restore H₂S homeostasis may be a critical approach for preventing and treating PD. In the present study, the H₂S donor sulforaphane (SFN) was investigated to elucidate its neuroprotective mechanisms through activation of the CBS–H₂S axis. siRNA-mediated silencing of Nrf2 and CBS was employed to clarify the role of each in SFN's effects. Our findings demonstrate that SFN promotes CBS expression and H₂S synthesis, activates mitophagy to clear damaged mitochondria, reduces mitochondrial-derived reactive oxygen species (mtROS) levels, and inhibits the activation of NLRP3 inflammasomes and caspase-1. In the MPTP-induced PD mouse model, SFN improved motor performance, increased the survival rate of tyrosine hydroxylase (TH)-positive dopaminergic neurons in the substantia nigra, and restored dopamine metabolism in the striatum, normalizing the DOPAC/DA and 5-HIAA/5-HT ratios. Electron microscopy revealed that SFN facilitated the clearance of damaged mitochondria through autophagosomes and blocked mtROS-mediated NLRP3 inflammasome activation. In the MPP + -induced BV-2 microglial cell model, SFN upregulated CBS expression, enhanced H₂S synthesis, increased the LC3-II/I ratio, and inhibited p62 degradation, thereby promoting the recovery of mitochondrial membrane potential and reducing ROS release. These effects were still observed under Nrf2 silencing conditions, indicating that SFN's neuroprotective effects are mediated through the CBS–H₂S axis independently of Nrf2 signaling. Collectively, these findings indicate that SFN reshapes the CBS–H₂S signaling axis, activates mitochondrial autophagy, and suppresses inflammation, offering novel insights into multi-target therapeutic approaches for PD and underscoring the essential role of H₂S in neuroprotection.
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高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Pengcheng Hu,Yongxin Li,Luchen Zhang,Xiaopeng Lan,Xiaochuan Ren,Wenlong Liang,Shen Wang,Yizhen Wang,Yanhui Zhao,Yanhui Zhang,Chunzhao Liu,Chunlei Liu
Cuproptosis is a newly recognized copper-dependent nonapoptotic form of cell death, which stimulate studies exploring copper-based nanomaterials to treat cancer through distinct mechanistic action. However, it remains a challenge to completely eradicate tumors via monotherapy. Herein, a copper-doped BiSe x (CBS) nanozyme was developed to boost αPD-L1-mediated immune checkpoint blocking (ICB) via synergetic apoptosis/cuproptosis-induced immunogenic cell death (ICD). The defect-engineered CBS nanozyme exhibits strong peroxidase-mimicking activities and generates abundant reactive oxygen species (ROS) production causing cell apoptosis, which could be further augmented by NIR photoirradiation. Meanwhile, the CBS could cause mitochondrial lipoylated protein aggregation, leading to cell cuproptosis. The photothermal/catalytic/cuproprosis synergistic therapy triggered by CBS nanozyme combined with αPD-L1 antibody effectively inhibits the growth of primary tumors and distant tumors in prostate cancer model. Furthermore, CBS nanozyme exhibited significant germicidal effect in wound infection. Collectively, this work provides a new insight into cancer treatment of copper-based nanozyme based on cuproptosis/apoptosis-related combined therapy.
研究领域:
癌症治疗
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纳米技术
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免疫疗法
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材料科学
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生物医学工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）