MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Shiwei Xu,Guofeng Wang,Yueyue Feng,Liying Huang,Zhongyu Yang,Yu Zhang,Jiacheng Dong,Yajun Wang,Nannan Liu
Gastric cancer (GC) is one of the leading causes of cancer-related mortality worldwide. miRNA-106a is a small non-coding RNA that is significantly up-regulated in GC cells. CD44 protein serves as a cell adhesion molecule in GC, and its high expression is associated with tumor invasiveness and metastatic potential. Detection of these two biomarkers for GC can significantly improve the sensitivity and specificity of tests, thus identifying patients more accurately and facilitating early diagnosis and intervention. In this study, we proposed a bottom-up assembly method of silica nanowires (SiNWs) modified glass microtube to construct a glass microsensor based on the functionalization of SiNWs-dual probes (SiNWs@DGM), which can be used for the simultaneous detection of the two gastric cancer markers mentioned above in a highly sensitive and specific manner. The detection limit of the sensor is as low as 1 aM for the target miRNA-106a and 1 pg/mL for CD44 protein. The constructed SiNWs@DGM demonstrates excellent selectivity, high anti-interference ability, specificity, and good storage stability in sample analysis without any amplification. Therefore, our biosensing strategy can reduce false positive and false negative results, providing more reliable support for gastric cancer management and ultimately improving patient prognosis and survival rates.
研究领域:
生物医学工程
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分子诊断学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
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2021
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IF 12.479
Xianqi Zhu,Lin Li,Jin Tang,Chunyu Yang,Hao Yu,Kunpeng Liu,Ziyan Zheng,Xinggui Gu,Qingsong Yu,Fu-Jian Xu,Zhihua Gan
Metastasis has been widely recognized as the most lethal threats for cancer patients . Due to their special genetic and environmental context, cancer stem cells (CSCs) which are resistant to most cytotoxic drugs and radiation, are considered as the dominant culprit for metastasis. Thus, the efficient targeting and thorough elimination of CSCs are significantly urgent for the enhancement of therapeutic efficacy. Herein, we developed a facile and smart photothermal-chemo therapeutic nano-assembly system, of which the surface was modified by a sheddable PEG shell and acid-activatable pro-penetration peptide, to surmount the physiological barriers in targeting CSCs. A highly-efficient diradical-featured croconium-based photothermal agent and a natural cytotoxic heat shock protein (HSP) inhibitor were co-loaded in redox-sensitive chitosan matrices to realize the synergistic photothermal-chemo therapy. Within solid tumors, the PEG shell that prevents the nano-assembly from mononuclear phagocytic clearance could rapidly leave to expose the positively charged chitosan, and the detached iRGD could further actuate the tumor penetration of chitosan nanoparticles , and allow the CSCs targeting by selective recognition of CD44 protein. Owing to the HSP inhibition and chemo-sensitization, both the CSCs and non-CSCs could be thoroughly eliminated by the designed nano-assembly, largely inhibiting the tumor growth and metastasis. This work provides a potential strategy for CSCs-targeting drug delivery to solve the CSCs-related metastasis.
研究领域:
肿瘤学
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纳米技术
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药物递送系统
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癌症生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Pharmaceutics-X
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2024
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IF 5.2
Lan Cheng,Yanhua Zhang,Qian Xu,Zheng Li,Zulan Liu,Fangyin Dai
Systemic administration of methotrexate (MTX), widely regarded as one of the most effective treatments for psoriasis, poses significant challenges due to its high toxicity, limited solubility, and potential for adverse effects. Consequently, developing a topical form of MTX may offer a safer and more effective strategy for psoriasis management. Silk fibroin (SF), a protein-based biomacromolecule, has shown considerable promise as a nanocarrier for sustained and targeted drug delivery, owing to its exceptional physicochemical and biological properties. This study aimed to develop and characterize a novel drug delivery nanocarrier for MTX using SF nanoparticles modified with hyaluronic acid (HA) and to assess their potential for skin-targeted drug delivery with reduced transdermal permeation. The nanoparticles were thoroughly characterized, demonstrating a uniform particle size, high drug-loading capacity, pH sensitivity, and excellent slow-release properties. In vitro and in vivo experiments further indicated that these nanoparticles effectively reduced psoriasis-induced inflammatory responses, including erythema and scaling, by inhibiting keratinocyte proliferation and decreasing levels of pro-inflammatory cytokines. The inclusion of HA improved nanoparticle targeting, particularly through interactions with overexpressed CD44 proteins in psoriatic skin, resulting in enhanced methotrexate accumulation at the sites of inflammation and improved therapeutic efficacy. Our findings suggest that HA/SF nanoparticles loaded with MTX represent a promising, safe transdermal delivery system for the localized treatment of psoriasis.
研究领域:
药学
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纳米技术
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皮肤病学
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药物递送系统
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炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2024
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IF 5.6
Shanshan Sun,Meng Su,Han Xiao,Xiaoshuang Yin,Ying Liu,Wenzhong Yang,Yun Chen
In this study, a self-powered biosensor based on an enzymatic biofuel cell was proposed for the first time for the ultrasensitive detection of soluble CD44 protein. The as-prepared biosensor was composed of the co-exist aptamer and glucose oxidase bioanode and bilirubin oxidase modified biocathode. Initially, the electron transfer from bioanode to biocathode was hindered due to the presence of the aptamer with high insulation, generating a low open-circuit voltage (E OCV ). Once the target CD44 protein was present, it was recognized and captured by the aptamer at the bioanode, thus the interaction between the target CD44 protein and the immobilized aptamer caused the structural change at the surface of the electrode, which facilitated the transfer of electrons. The E OCV showed a good linear relationship with the logarithm of the CD44 protein concentrations in the range of 0.5–1000 ng mL −1 and the detection limit was 0.052 ng mL −1 (S/N = 3). The sensing platform showed excellent anti-interference performance and outstanding stability that maintained over 97% of original E OCV after 15 days. In addition, the relative standard deviation (1.40–1.96%) and recovery (100.23–101.31%) obtained from detecting CD44 protein in real-life blood samples without special pre-treatment indicated that the constructed biosensor had great potential for early cancer diagnosis.
研究领域:
生物化学
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生物传感器
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癌症诊断
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
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2021
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IF 15.881
Bin Liu,Yulong Bian,Shuang Liang,Meng Yuan,Shuming Dong,Fei He,Shili Gai,Piaoping Yang,Ziyong Cheng,Jun Lin
Recently, various metal peroxide nanomaterials have drawn increasing attention as an efficient hydrogen peroxide (H2O2) self-supplying agent for enhanced tumor therapy. However, a single kind of metal peroxide is insufficient to achieve more effective antitumor performance. Here, a hyaluronic acid modified calcium and copper peroxides nanocomposite has been synthesized by a simple one-step strategy. After effective accumulation at the tumor site due to the enhanced permeability and retention (EPR) effect and specific recognition of hyaluronate acid with CD44 protein on the surface of tumor cells, plenty of Ca2+, Cu2+, and H2O2 can be simultaneously released in acid and hyaluronidase overexpressed tumor microenvironment (TME), generating abundant hydroxyl radical through enhanced Fenton-type reaction between Cu2+ and self-supplying H2O2 with the assistance of glutathione depletion. Overloaded Ca2+ can lead to mitochondria injury and thus enhance the oxidative stress in tumor cells. Moreover, an unbalanced calcium transport channel caused by oxidative stress can further promote tumor calcification and necrosis, which is generally defined as ion-interference therapy. As a result, the synergistic effect of Fenton-like reaction by Cu2+ and mitochondria dysfunction by Ca2+ in ROS generation is performed. Therefore, a TME-responsive calcium and copper peroxides nanocomposite based on one-step integration has been successfully established and exhibits a more satisfactory antitumor efficiency than any single kind of metal peroxide.
研究领域:
癌症治疗
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纳米技术
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）