BIOSENSORS & BIOELECTRONICS
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2024
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IF 10.7
Chuanyu Yang,Caiyi Du,Feiyu Yuan,Peitong Yu,Boxu Wang,Changshun Su,Ruiqi Zou,Junyang Wang,Xu Yan,Chunyan Sun,Hongxia Li
Benefiting from specific target recognition and trans-cleavage capabilities, the CRISPR/Cas12a system has great application prospects in the design of highly sensitive and rapid fluorescence biosensors. The CRISPR/Cas12a-based fluorophore-quencher molecular beacons exhibit single-color emission and are easily exposed to interference from environmental factors. Herein, we design a CRISPR/Cas12a-derived ratiometric fluorescence sensor for Pb 2+ detection based on embedded carbon dots@zeolitic imidazolate framework-8 (CDs@ZIF-8) composites and DNAzyme. The functions of ZIF-8 about encapsulating red emissive CDs in the inner cavity and adsorbing DNA on the outer surface are integrated to establish dual fluorescence signals, thereby reducing the possibility of interference and improving sensing accuracy. The presence of Pb 2+ is converted into the change of activator by the GR5 DNAzyme to activate the CRISPR/Cas12a system, which provides signal amplification through multiple turnovers of side branch cutting, achieving highly sensitive detection of Pb 2+ with a low detection limit of 18 pM. This method has the advantages of simplicity, universality, and excellent quantitative ability, and has broad prospects in sensing applications.
研究领域:
生物传感
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分子生物学
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环境科学
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PubMed
高影响因子
RSC Advances
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2017
·Vol. 7
·期 50
·31365-31371
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IF 3.108
Rong-Mei Kong,Yan Zhao,Yiqun Zheng,Fengli Qu
Zeolitic imidazolate frameworks (ZIF) are attracting considerable attention for their applications in adsorbents/separation as well as catalysis. In this article, we proposed a simple strategy to fabricate semiconductor–ZIF core–shell nanocomposites, which exhibited remarkable coherent function for adsorption and photocatalytic degradation of organic pollutants. The current nanocomposites were composed of ZnO/CdS and ZIF-8, where spindle-like ZnO nanoparticles not only acted as the seeding material but also worked as the Zn2+ source for the formation of ZIF-8. The as-prepared ZnO/CdS@ZIF-8 core–shell nanocomposites displayed distinct photocatalytic activity for methylene blue (MB) and rhodamine B (RhB) owing to the unique pore structure and thus absorption effect of the ZIF-8 shell. To this end, the ZnO/CdS@ZIF-8 core–shell nanocomposites could find important applications in photocatalytic degradation of pollutants.
研究领域:
材料科学
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环境科学
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高影响因子
CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Miao Miao,Lan Mu,Shaomei Cao,Yuhuan Yang,Xin Feng
Tetracycline (TC) residues have been noted as an important class of emerging contaminants in the environment, thus fast, straightforward, highly sensitive detection method is becoming an issue that must be addressed. Herein, a novel CDs@ZIF-8 sensing material was fabricated by encapsulating luminescent carbon dots (CDs) into the metal-organic framework (ZIF-8) to achieve highly luminescent porous composites. Furthermore, to avoid the secondary contamination caused by powders, a shapable CDs@ZIF-8/CS film sensor as a portable disposable test strip with dual-function of TC detection and adsorption was designed by crosslinking CDs@ZIF-8 with chitosan (CS). The as-prepared CDs@ZIF-8/CS hybrid film exhibited the high sensitivity and selectivity for TC fluorescence detection and rapid decontaminate capability, as well as high optical transmittance and robust mechanical property, which were essential for the practical sensing application. This paper-based dual-functional luminescent sensor exhibits promising practicability for TC detection and contaminant removal in pharmaceutical analysis, food safety and water treatment.
研究领域:
环境科学
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分析化学
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材料科学
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高影响因子
Advanced Science
·
2025
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IF 14.1
Yongrui Ti,Ziqiang Qin,Xiaohong Guo,Zheng Wu,Qi Jiang,Beihua Dong,Olaniyi Amos Fawole,Di Wu,Su Chen,Xian Li,Kunsong Chen
Carbon dots (CDs) have elicited attention in photodynamic antimicrobial applications, but their susceptibility to aggregation limits their effectiveness. Herein, CDs@ZIF-8 nanocomposites are synthesized. The results reveal that they have superior dual photodynamic enhanced antimicrobial properties, specifically 7.63 log 10 CFU mL −1 (lg) elimination for Escherichia coli (only 3.12 lg with CDs and 4.18 lg with ZIF-8) and 7.27 lg elimination for Staphylococcus aureus (only 4.17 lg with CDs and 2.94 lg with ZIF-8). Optoelectronic property analysis and density functional theory calculations show that the enhanced photodynamic antimicrobial properties of CDs@ZIF-8 can be attributed to their narrow bandgap and higher binding affinity for O 2 , which facilitates the photo-generated e − –h + pairs separation and promotes the generation of 1 O 2 and •O 2 − . Furthermore, the CDs@ZIF-8/polycaprolactone/ethyl cellulose nanofibrous films are prepared by microfluidic-blow-spinning, which have prominent antimicrobial properties and extend the shelf life of strawberries from 6 to 12 d. Overall, this study provides a novel idea for designing synergistically enhanced photodynamic antimicrobial materials and photodynamic preservation of perishable fruits.
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高影响因子
CHEMICAL ENGINEERING SCIENCE
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2025
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IF 4.3
Meiling Xu,Linchun Nie,Jiawei Liao,Dejing Meng,Wenjing Liu,Haobo Yang,Ranhao Zhao,Qingxiang Zhou
A novel and visible ratiometric fluorescence sensor based on magnesium and nitrogen co-doped carbon dots (Mg, N-CDs) encapsulated in zeolitic imidazolate framework-8 (Mg, N-CDs@ZIF-8) was designed for effective detection of oxytetracycline (OTC), employing a “turn-off/turn-on” fluorescence sensing strategy. The blue-emission Mg, N-CDs@ZIF-8 was quenched owing to fluorescence resonance energy transfer by OTC, while the green fluorescence of OTC was significantly enhanced through the interaction of Mg 2+ and Zn 2+ with OTC, enhancing the visual tracking of OTC through a distinct blue-to-green color transition. This sensor displayed a wide linearity range (0.02 − 25 μg/mL), a low detection limit (14.2 ng/mL), and a fast response time (6 min). The validity of the sensor was successfully verified by detecting OTC in milk, honey, tap water, and lake water samples. Additionally, an expandable fluorescence sensor array was constructed for the discrimination of four tetracycline antibiotics (TCs) in real samples, indicating significant application in complex environmental samples.
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高影响因子
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