ELECTROPHORESIS
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2016
·Vol. 37
·期 14
·2097-2105
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IF 2.482
Ya-Jun Duan,Li-Yun Zhang,Xiao-Feng Guo,Hong Wang
A rapid and robust CE method using a long wavelength fluorescent reagent 1,7-dimethyl-3,5-distyryl-8-phenyl-(2-maleimide)difluoroboradiaza- s -indacene as the labeling reagent has been developed for the simultaneous determination of thiols, including glutathione, cysteine, homocysteine, N -acetylcysteine, cysteinylglycine, and penicillamine. The derivatization reaction was carried out in 14 mmol/L pH 8.5 borate buffer at 30°C for 6 min and the labeled thiols derivatives were separated with the running buffer containing 30 mmol/L pH 7.4 phosphate, 30% v/v acetonitrile and 8 mmol/L SDS within 12 min. Detection limits ranged from 0.4 to 2.4 nmol/L. To demonstrate the capability of this method, it was applied to the analysis of thiols in human urine with recoveries of 92.4–105.6%. The derivatization reaction was much faster at milder conditions, and the analysis was rapider. Moreover, with excitation wavelength at long wavelength region, background interference from samples was reduced effectively. The present method seems to be a potential choice for quantifying thiols in human urine.
研究领域:
分析化学
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生物化学
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DOI
PubMed
高影响因子
Food Bioscience
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2025
·
IF 5.9
Ziru Feng,Mengmeng Yan,Tengfei Li,Wenjun Zhang,Chao Zhu
Lactoferrin (Lf) is closely associated with the growth of infants and children, and its recognition is imperative due to its rising application as a prevalent nutritional fortification in infant formula. In this study, an ssDNA aptamer was screened out that enabled sandwich recognition of Lf by forming an aptamer pair with another available aptamer. During the process, the reported aptamer (SeqT1.1) was selected as the substrateto be linked onto pre-blocked microplates for capturing Lf, followed by the addition of the ssDNA library, resulting in the formation of the sandwich complex of the “SeqT1.1/Lf/ssDNA library” that was subsequently collected for screening. After seven rounds, we discovered a new ssDNA aptamer (Seq6T) through a comprehensive analysis involving sequence frequency, secondary structures, homology, and sequence truncation, which helped address the binding interference between aptamer pairs with each other’s binding. The Seq6T demonstrated good specificity and low-nanomolar affinity by ELONA (K D , 12.59 nM) and CE-LIF (K D , 22.44 nM) assays, which illustrated the potential of applying that in both homogeneous and non-homogeneous environments. The sandwich recognition of “SeqT1.1/Lf/Seq6T” was further verified by molecular docking and ELONA, with a good detection limit of 18.35 nM. The aptamer pairs provided a considerable basis for accelerating the sandwich recognition applications towards Lf.
研究领域:
生物技术
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分子生物学
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食品科学
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分析化学
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2021
·
IF 3.365
Xiao Yu,Wanxiang Yu,Xiaohui Zhang,Yongjie Wang,Shumei Wang,Haiyun Zhai
Based on advantages of capillary electrophoresis (CE), a new solid-phase extraction (SPE) coupled with CE has been developed for preconcentration , enrichment and determination of anthraquinones and flavonoids (rutin, emodin , quercetin , 1,8-dihydroxyanthraquinone) in honey. The environmental-friendly chitin activated after an easy processing is selected as the adsorbent to enrich analytes. Then, chitin was filled into the filter as the solid phase. To improve the extraction effect, some key parameters of extraction were optimized. Under the optional extraction conditions, the chitin showed excellent adsorption capacity and selectivity over rutin , emodin, quercetin, and 1,8-dihydroxyanthraquinone, with enrichment factors reaching 5 folds. The CE coupled with fluorescence detection was used for the detection. Results prove the method is simple, fast, and highly sensitive, with the limit of detection (LOD) is 3.00–200.0 ng/mL; the recovery is 90.0–107.0%, and relative standard deviation of (RSD) is 1.8–8.3%.
研究领域:
分析化学
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食品科学
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药物分析
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DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 24
·2964-2970
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IF 3.1
Shaoyan Zhang,Jinfeng Ning,Qingqing Wang,Wei Wang
Flavonoids are widely used in the treatment of various diseases due to their antioxidant, anti-inflammatory, anticancer and antiviral properties. Fluorescence detection is rarely applied for the determination of flavonoids because of their weak fluorescence. In this work, a method of fluorescence enhancement of flavonoids was firstly introduced by using sodium acetate for flavonoid derivatization. The study discovered that flavonoids, with a hydroxyl at the C3 position, had the ability to emit strong fluorescence after derivatization. Five flavonoids, kaempferide, galangin, isorhamnetin, kaempferol and quercetin, having a special structure, were selected, derivatized and analyzed by capillary electrophoresis with laser-induced fluorescence detection. Under the optimal conditions, the five flavonoids could be completely separated within 3 minutes. Good linear relationships were obtained for all analytes and the limits of detection for the five flavonoids were in the range of 1.18–4.67 × 10−7 mol L−1. Finally, the method was applied to the determination of flavonoids in five traditional Chinese medicines: aster, chamomile, galangal, tangerine peel and cacumen biotae. Flavonoids were successfully found in all these medicines by the developed method. The recoveries were in the range of 84.2–111%. The method developed in this study was fast, sensitive and reliable for the determination of flavonoids.
研究领域:
分析化学
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药学
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植物化学
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DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2012
·Vol. 34
·期 3
·409-416
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IF 3.303
Juan Qiao,Li Qi,Huijuan Yan,Yaping Li,Xiaoyu Mu
l -Asparaginase ( l -Asnase) can suppress the growth of malignant cells by rapid depletion of two essential amino acids, l -glutamine ( l -Gln) and l -asparagine ( l -Asn). To study the cytotoxic effect and the secondary complications of l -Asnase in the treatment of acute lymphoblastic leukemia, the development of a novel enzyme reactor of l -Asnase for the hydrolysis of l -Gln, employing the enzyme-gold nanoparticle conjugates in capillary, was reported in this work. First, a microchip CE (MCE)-LIF was established for the separation of l -amino acids ( l -Gln and l -glutamic acid) and studying the hydrolysis of l -Gln by using l -Asnase enzyme reactor. Then, using l -Gln as target analyte, the enzyme kinetics of l -Asnase in free solution, enzyme-gold nanoparticle conjugates (E-GNP), and the enzyme-gold nanoparticle conjugates immobilized in capillary (E-GNP-C) were investigated in detail with the proposed MCE-LIF method. Moreover, for optimizing the enzymatic reaction efficiency, three important parameters, including the length of capillary, the enzyme concentration reacted with gold nanoparticle and the amount of l -Asnase immobilized on the gold nanoparticle, have been studied. Owing to the high specific activity, the E-GNP-C enzyme reactor exhibited the best performance for the hydrolysis of l -Gln.
研究领域:
生物化学
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医学
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纳米技术
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jingtong Xia,Yanxia Zhang,Tao Wang,Jiaying Ran,Hongli Li,Lei Zhou,Qiaosheng Pu
Abnormal glucose metabolism has been identified as a key characteristic of tumorigenesis. Visualizing the levels of monosaccharides in the lesion tissues and bodies of cancer patients is conducive to uncovering patterns of glucose metabolic reprogramming and assisting in the early diagnosis of cancer. Consequently, based on 7-(diethylamino)coumarin-3-carbohydrazide (DCCH), which has mass spectrometry/optical dual-signal enhancement functionality, both an on-tissue derivatization strategy suitable for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) analysis and an in-solution derivatization strategy for capillary electrophoresis/laser-induced fluorescence (CE-LIF) analysis have been developed. During the on-tissue derivatization reaction, the nonvolatile MALDI matrix, 2,5-dihydroxybenzoic acid, was innovatively adopted as a proton donor. This approach not only efficiently catalyzes the derivatization process but also significantly minimizes analyte redistribution, and a low-cost airbrush application can achieve satisfying in situ derivatization. For further addressing the issue of the coexistence of multiple conformational isomers in biosamples, a rapid and efficient CE-LIF method was proposed for the separation of five DCCH-labeled monosaccharides (including three conformational isomers) within 8 min. By focusing on endometrial cancer (EC), the synergy of DCCH-mediated MALDI-MSI and CE-LIF analysis successfully achieved high-resolution in situ visualization of an obvious depletion difference in monosaccharides across the focal areas (tumor tissues, peritumoral tissues, and paracancerous tissues), and a comparative analysis was conducted on the serum monosaccharide profiles between patients with EC and those with benign uterine diseases. These analyses yielded novel molecular-level evidence, thereby facilitating a deeper understanding of the monosaccharide metabolic abnormalities associated with EC.
研究领域:
癌症代谢
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分析化学
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分子成像
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临床诊断
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糖组学
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质谱分析
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电泳技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2018
·
IF 0.827
Liwei Cao,Tian Wei,Yihui Shi,Xiaofang Tan,Jianxin Meng
Two rapid and sensitive analytical methods are developed for the determination of D-penicillamine (D-PEN) and tiopronin (TP) through high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). A boron-dipyrrolemethene (BODIPY) fluorescence labeling reagent, 1,3,5,7-tetramethyl-8-bromomethyl-difluoroboradiaza-s-indacene (TMMB-Br) was successfully applied to derivatize these two thiol drugs. Fluorescein was used as the internal standard (IS) for the quantification of D-PEN and TP in CE-LIF. The derivatization and separation conditions were optimized carefully. Under the optimum conditions, the HPLC and CE separation of D-PEN and TP could be achieved within 12 min. The limits of detection were as low as 2.0 nmol/L for HPLC-FLD and 0.47 nmol/L for CE-LIF. The drugs in human urine and serum samples were determined successfully, and the recoveries were 95.0–06.7% and 95.2–104.3%, respectively.
研究领域:
分析化学
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药物分析
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生物医学
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DOI
高影响因子
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