ChemistrySelect
·
2024
·Vol. 9
·期 24
·e202400499
·
IF 1.9
Jing Wang,Lu Ren,Chang Liu,Yanqi Liu,Yujie Shang,Yulu Zhang,Dawei Zhang
Graphical Two imidazole-based color-changing fluorescent probes 4-(4,5-bis(4-chlorophenyl)-1 H -imidazol-2-yl)- N , N -diphenylaniline (CIN) and 4-(4,5-di(furan-2-yl)-1 H -imidazol-2-yl)- N , N -diphenylaniline (FIN) were synthesized and characterized. The new probes CIN and FIN exhibit excellent selectivity and sensitivity towards Fe 3+ with relatively low detection limits and strong anti-interference properties. Both the probes CIN and FIN complexed 1 : 1 with Fe 3+ ion. Two imidazole-based color-changing fluorescent probes 4-(4,5-bis(4-chlorophenyl)-1 H -imidazol-2-yl)- N , N -diphenylaniline (CIN) and 4-(4,5-di(furan-2-yl)-1 H -imidazol-2-yl)- N , N -diphenylaniline (FIN) were synthesized and characterized. The new probes CIN and FIN exhibit excellent selectivity and sensitivity towards Fe 3+ with relatively low detection limits (0.26 μM and 0.10 μM) and strong anti-interference properties. Both the probes CIN and FIN complex 1 : 1 with Fe 3+ ion, and the complexation constants were 1.165×10 5 M −1 (CIN) and 1.899×10 5 M −1 (FIN), respectively. Moreover, CIN and FIN could be recycled by EDTA and could effectively monitor Fe 3+ ions at pH 4–10, which ensures the sensor‘s potential for detecting Fe 3+ in actual aqueous solutions. The probes were further applied to the detection of Fe 3+ in the actual reaction of Fe 2+ oxidation, and the filter paper and the probe solution are combined to make an indicator to detect Fe 3+ more conveniently and quickly.
研究领域:
化学传感器
·
分析化学
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产品关联
DOI
高影响因子
PLoS One
·
2016
·Vol. 11
·期 10
·e0163340
·
IF 3.057
Hong Zhou,Yapei Wang,Qiongying Lv,Juan Zhang,Qing Wang,Fei Gao,Haoli Hou,Hao Zhang,Wei Zhang,Lijia Li
The ribosomal RNA (rRNA) gene encodes rRNA for protein synthesis. Aberrant expression of the rRNA gene has been generally observed in tumor cells and levels of its promoter methylation as an epigenetic regulator affect rRNA gene transcription. The possible relationship between expression and promoter methylation of rDNA has not been examined in human clinical cervical cancer. Here we investigate rRNA gene expression by quantitative real time PCR, and promoter methylation levels by HpaII/MspI digestion and sodium bisulfite sequencing in the development of human cervical cancer. We find that indeed rRNA levels are elevated in most of cervical intraepithelial neoplasia (CIN) specimens as compared with non-cancer tissues. The rDNA promoter region in cervical intraepithelial neoplasia (CIN) tissues reveals significant hypomethylation at cytosines in the context of CpG dinucleotides, accompanied with rDNA chromatin decondensation. Furthermore treatment of HeLa cells with the methylation inhibitor drug 5-aza-2’-deoxycytidine (DAC) demonstrates the negative correlation between the expression of 45S rDNA and the methylation level in the rDNA promoter region. These data suggest that a decrease in rDNA promoter methylation levels can result in an increase of rRNA synthesis in the development of human cervical cancer.
研究领域:
分子生物学
·
肿瘤学
·
表观遗传学
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DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Zhi-Yuan Nie,Xue-Ying Li,Ying-Ying Chen,Yuan-Yuan Deng,Dan-Dan Kuang,Yu-Lin Sun,Huan-Huan Xiao,Qiang-Ming Li,Jian-Ping Luo,Xue-Qiang Zha
In this study, a novel self-assembled micelle (Myr-OSADS-CIn) based on OSA-debranched lotus root starch (OSADS) and cationic inulin (CIn) was successfully constructed to enhance the stability and bioavailability of myricetin (Myr). Firstly, lotus root starch and inulin were chemically modified. The results of Fourier transform infrared spectroscopy, X-ray diffraction, Nuclear magnetic resonance spectroscopy and Element analysis confirmed the successful synthesis of OSADS and CIn. When the optimal weight ratio of Myr/OSADS/CIn was 1:40:120, a stable Myr-OSADS-CIn composite micelle was successfully formed. The key characteristics of the micelle included a polydispersity index (PDI) of 0.321, a zeta-potential of +24.247 mV, and an encapsulation efficiency of 87.536%. Transmission electron microscopy analysis revealed unique core-shell structures within the Myr-OSADS-CIn micelle. The formation and stability of the composite micelle were primarily influenced by hydrogen bonds and electrostatic interactions. Notably, the Myr-OSADS-CIn micelle exhibited excellent biocompatibility, significant antioxidant activity and controlled release of Myr. Furthermore, in vivo bioavailability of Myr in the Myr-OSADS-CIn micelle was found to be 4.054 times higher compared to free Myr. In conclusion, the Myr-OSADS-CIn micelle shows promise as a delivery system for the effective use of Myr in the food industry.
研究领域:
药学
·
食品科学
·
材料科学
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DOI
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Xiong Jinfeng,Li Guannan,Mei Xinyu,Ding Jiahui,Shen Hui,Zhu Da,Wang Hui
The p53 gene has the highest mutation frequency in tumors, and its inactivation can lead to malignant transformation, such as cell cycle arrest and apoptotic inhibition. Persistent high-risk human papillomavirus (HR-HPV) infection is the leading cause of cervical cancer. P53 was inactivated by HPV oncoprotein E6, promoting abnormal cell proliferation and carcinogenesis. To study the treatment of cervical intraepithelial neoplasia (CIN) and cervical cancer by restoring p53 expression and inactivating HPV oncoprotein, and to verify the effectiveness of nano drugs based on nucleic acid delivery in cancer treatment, we developed poly (beta-amino ester)537, to form biocompatible and degradable nanoparticles with plasmids (expressing p53 and targeting E7). In vitro and in vivo experiments show that nanoparticles have low toxicity and high transfection efficiency. Nanoparticles inhibited the growth of xenograft tumors and successfully reversed HPV transgenic mice’s cervical intraepithelial neoplasia. Our work suggests that the restoration of p53 expression and the inactivation of HPV16 E7 are essential for blocking the development of cervical cancer. This study provides new insights into the precise treatment of HPV-related cervical lesions.
研究领域:
肿瘤学
·
分子生物学
·
纳米技术
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基因治疗
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
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