Journal of Tissue Engineering and Regenerative Medicine
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2015
·Vol. 11
·期 5
·1562-1573
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IF 5.199
Quan Liu,Yuchen Huang,Yong Lan,Qinhua Zuo,Chenghua Li,Yi Zhang,Rui Guo,Wei Xue
Two important issues in skin tissue engineering are the vascularization and regeneration of the dermis. Basic fibroblast growth factor (bFGF) is known to promote angiogenesis and accelerate wound healing. Direct delivery of bFGF to the wound area, however, would lead to a loss of bioactivity. To this end, bFGF-loaded alginate microspheres (Ms) were fabricated and incorporated into carboxymethyl chitosan (CMCS)–poly(vinyl alcohol) (PVA) to form a composite hydrogel. Scanning electron microscopy (SEM) results indicated that the incorporation of Ms does not significantly affect the inner structure of CMCS–PVA. In an in vitro study, the release of bFGF from Ms–CMCS–PVA in a sustained manner retained higher bioactivity over a 2-week period. Full-thickness burn wounds were created in the dorsal area of rats for in vivo evaluation of skin regeneration treated with CMCS–PVA hydrogel, with and without bFGF. Compared with the control, CMCS–PVA and bFGF–CMCS–PVA groups, the bFGF/Ms–CMCS–PVA group revealed significantly faster wound recovery rates, with re-epithelialization and regeneration of the dermis. Moreover, the bFGF/Ms–CMCS–PVA group had the highest density of newly formed and mature blood vessels during the 2 mweek treatment period. The ability of the bFGF/Ms–CMCS–PVA hydrogel to accelerate wound healing in a full-thickness burn model suggests its potential for use in dermal tissue regeneration. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
生物材料学
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组织工程学
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医学
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PubMed
高影响因子
CURRENT MICROBIOLOGY
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2023
·Vol. 80
·期 9
·1-9
·
IF 2.6
Jiao Yu Liang,Shen Pin Quan,Wang Shu Fang,Chen Jing,Zhou Xiang Hong,Ma Gui Zhen
Arginase has shown promising potential in treating cancers by arginine deprivation therapy; however, low enzymatic activity and stability of arginase are impeding its development. This study was aimed to improve the enzymological properties of a marine bacterial arginase by carboxymethyl chitosan (CMCS) conjugation. An arginase producing marine bacterium Priestia megaterium strain P6 was isolated and identified. The novel arginase PMA from the strain was heterologously expressed, purified, and then conjugated to CMCS by ionic gelation with calcium chloride as the crosslinking agent. Enzymological properties of both PMA and CMCS-PMA conjugate were determined. The optimum temperature for PMA and CMCS-PMA at pH 7 were 60 °C and 55 °C, respectively. The optimum pH for PMA and CMCS-PMA at 37 °C were pH 10 and 9, respectively. CMCS-PMA showed higher thermostability than PMA over 55–70 °C and higher pH stability over pH 4–11 with the highest pH stability at pH 7. At 37 °C and pH of 7, i.e., around the human blood temperature and pH, CMCS-PMA was higher than the free PMA in enzymatic activity and stability by 24% and 21%, respectively. CMCS conjugation not only changed the optimum temperature, optimum pH, and enzymatic activity of PMA, but also improved its pH stability and temperature stability, and thus made it more favorable for medical application.
研究领域:
生物化学
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生物技术
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药学
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癌症研究
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PubMed
高影响因子
International Journal of Nanomedicine
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2018
·
IF 4.37
Fei Tong,Suhuan Liu,Bing Yan,Xuejun Li,Shiwei Ruan,Shuyu Yang
Introduction Insulin has shown antioxidation and cytoprotective effects to decrease heart ischemia/reperfusion injury (HI/RI) in diabetes mellitus (DM), but the role of insulin/poly(ethylene glycol)-carboxymethyl chitosan (PEG-CMCS) on HI/RI in DM is not known. This research explored whether insulin/PEG-CMCS revealed a protective effect on HI/RI in DM through ornithine decarboxylase (ODC)/polyamine systems.Materials and methods Diabetes was induced via streptozotocin (STZ) in Sprague Dawley (SD) rats, which suffered from HI via blocking the left circumflex artery for 45 minutes, followed by 2 hours of reperfusion. α-Difluoromethylornithine-ethylglyoxal bis (guanylhydrazone) (DFMO-EGBG) and insulin/PEG-CMCS were administered to diabetic rats to explore their roles on severity of HI/RI.Results Insulin could be fleetly and efficiently loaded via the nanocarrier PEG-CMCS at pH =6, showing efficient loading and stable release. In addition, insulin/PEG-CMCS showed significant hypoglycemic activity in diabetic rats. On the other hand, ischemia/reperfusion obviously augmented the contents of creatine kinase (CK), lactic dehydrogenase (LDH), putrescine (Pu), myocardial infarct size, and NF-κB and spermidine/spermine N′-acetyltransferase (SSAT) expressions and decreased the levels of spermine (Sp), polyamine pools (PAs), heart rate (HR), coronary blood flow (CF), left ventricular developed pressure (LVDP), and ODC expression, compared with Sham. Administration of insulin and insulin/PEG-CMCS both reduced the contents of CK, LDH, Pu, myocardial infarct size, and NF-κB and SSAT expressions and increased the levels of Sp, PAs, HR, CF, LVDP, and ODC expression, while insulin/PEG-CMCS significantly indicated the protective results, and DFMO-EGBG showed the opposite effects.Conclusion The research showed that insulin/PEG-CMCS could play a protective effect on HR/RI in diabetic rats via its antioxidative, antiapoptotic, and anti-inflammatory roles and modulating ODC/polyamine systems.
研究领域:
药理学
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分子生物学
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心血管病学
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PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2022
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IF 3.141
YuLiang Jiao,YuYing Zhu,ShuMin Zeng,ShuFang Wang,Jing Chen,XiangHong Zhou,GuiZhen Ma
A novel uricase producing marine bacterium Priestia flexa alkaAU was isolated and identified. The 16S rDNA and the uricase coding gene were sequenced, analyzed and submitted to GenBank. The uricase from Priestia flexa alkaAU (PFU) was purified, determined to be 58.87 kDa, and conjugated with carboxymethyl chitosan (CMCS) by ionic gelation. CMCS conjugation had no effect on the optimum pH of PFU but decreased the optimum temperature by 10 °C. CMCS conjugation increased the specific activity of PFU by 53% at the human body temperature (37 °C) and small intestine’s pH (pH 6.8). Uricase thermostabilizing ability of CMCS was significant in the range of 37–80 °C but not at lower temperatures. For improvement of the pH stability of PFU, CMCS was more effective at pHs 3–5 than pHs 6–11. CMCS increased the half-life of PFU against artificial intestinal fluid by 1.5 folds, which demonstrated the potential capability of CMCS–PFU for oral administration.
研究领域:
海洋微生物学
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酶学
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生物技术
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药学
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PubMed
高影响因子
Frontiers in Sustainable Food Systems
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2023
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IF 4.7
Mo Feixu,Long Youhua,Li Ming,Ding Yi,Wu Shuai,Li Rongyu
To find a precise, efficient, and safe method to control rice sheath blight disease (caused by Rhizoctonia solani), this study used the natural product magnolol as the fungicide. Based on the characteristics of cell wall-degrading enzymes (CWDEs) released by R. solani during the early stages of infection, a Mag@CMCS@CPSG (magnolol@carboxymethyl chitosan@carboxymethyl cellulose, pectin, sodium alginate, and glycerol) film was constructed to control the release of magnolol in response to CWDEs. The Mag@CMCS@CPSG film increased magnolol toxicity against R. solani, decreased EC50 from 7.33 μg/mL to 4.37 μg/mL, and effectively blocked the invasion of mycelia. Under the stimulation of CWDEs released during R. solani infection, the cumulative release of magnolol by the Mag@CMCS@CPSG film reached 75.62% after 96 h, and the disease index of rice treated with Mag@CMCS@CPSG film significantly decreased. The maximum field control effect of Mag@CMCS@CPSG film on rice sheath blight disease was 72.47%, which was not significantly different from that of the commercial preparation jinggangmycin; however, the holding period was better. The Mag@CMCS@CPSG film reduced the loss of rice yield and quality caused by infection with R. solani and did not cause phytotoxicity or residue. In conclusion, the Mag@CMCS@CPSG film can achieve accurate and green control of rice sheath blight.
研究领域:
植物病理学
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农业生物技术
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材料科学
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DOI
高影响因子
BIOELECTROCHEMISTRY
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2025
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IF 4.5
Jintao Liang,Shuaikang Dong,Xiaohong Tan,Junyan Gong,Xuyang Chen,Zhide Zhou,Guiyin Li
Cardiovascular disease (CVD) remains a significant worldwide health challenge, with mortality rates rising rapidly. Low-density lipoprotein (LDL) is a crucial serum biomarker for the early diagnosis of CVD, which can significantly improve outcomes and reduce mortality. Herein, a label-free electrochemical aptasensor for rapid detection of LDL was developed based on the titanium carbide–carboxymethyl chitosan–hemin (MXene-CMCS-Hemin) nanocomposites as the electrochemical signal probe. Firstly, gold nanoparticles (Au NPs) were electrodeposited onto a screen-printed carbon electrode (SPCE) to form a conductive substrate. Subsequently, the MXene-CMCS-Hemin nanocomposites were anchored onto the Au NPs/SPCE surface. Then LDL Apt was immobilized on the surface of MXene-CMCS-Hemin/Au NPs/SPCE to construct the electrochemical aptasensor. When LDL specifically bound with the LDL Apt to form LDL-LDL Apt complexes, hindering the electron transfer and reducing the Hemin oxidation current, LDL detection can be achieved via differential pulse voltammetry (DPV). Under optimal circumstances, the changes of Hemin's oxidation current showed a good linear response with LDL concentration in the range of 0.1–4.0 μmol/L with a detection limit of 0.095 μmol/L (S/N = 3). The aptasensor demonstrated good performance with the relative errors of 0.60 % to 6.58 % for the direct detection of LDL in human serum samples, which offers a novel tool for the clinical diagnosis of CVD.
研究领域:
生物医学工程
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分析化学
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临床诊断
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PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2018
·
IF 1.911
Feng Su,Yuandou Wang,Xue Liu,Xin Shen,Xingjian Zhang,Quansheng Xing,Lihong Wang,Yangsheng Chen
Carboxymethyl chitosan-graft-polylactide (CMCS-PLA) and carboxymethyl chitosan (CMCS) hydrogels were prepared by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) as crosslinking agent and catalyst at room temperature. The biocompatibility of the hydrogels was evaluated with the aim of assessing their potential as drug carrier. Various aspects of biocompatibility were considered, including MTT assay, agar diffusion test, release of lactate dehydrogenase (LDH), hemolytic test, plasma recalcification time (PRT), and dynamic clotting time. MTT assay showed that the cytotoxicity level of both hydrogels to L-929 cells was 0 or 1. The LDH release of CMCS and CMCS-PLA was 26 and 29%, respectively, which is slightly higher than that of the negative control (21%) and much lower than that of the negative control (87%). The hemolysis ratio of CMCS and CMCS-PLA was 1.4 and 1.7%, respectively, suggesting outstanding anti-hemolysis properties of both materials. The PRT value of CMCS and CMCS-PLA was higher by 77 and 99% than the value of the positive control. All the results revealed that the hydrogels present good cytocompatibility and hemocompatibility in vitro. In vivo degradation and tissue compatibility were evaluated by subcutaneous injection in the dorsal area of rats. CMCS and CMCS-PLA hydrogels were completely degraded and the inflammatory response also completely disappeared around hydrogels after 19 days in vivo. It is thus concluded that hydrogels formed of CMCS and CMCS-PLA with outstanding biocompatibility are promising as potential drug carrier.
研究领域:
生物材料
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药理学
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生物医学工程
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PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2020
·
IF 5.88
Tao Chen,Qingxia Zou,Chang Du,Chunren Wang,Yan Li,Bufang Fu
Three-dimensional (3D) printing technology has attracted considerable focus for preparing porous bone repair scaffolds to promote bone regeneration. Inspired by organic-inorganic components and the porous structure of natural bone, novel porous degradable scaffolds have been printed using hydroxyapatite (HA), carboxymethyl chitosan (CMCS), and polydopamine (PDA). The well-designed HA/CMCS/PDA scaffolds exhibited a porous structure with 60.5 ± 4.6% porosity and 415 ± 87 μm in mode pore diameter. The weight loss percentage (WL%) of the HA/CMCS/PDA scaffolds reached about 17% during a 10-week degradation in vitro . The degradation process between the CMCS and HA induced the release of calcium ions. Using commercial product as the contrast material, the osteogenic properties of the scaffolds were assessed in vivo . The implantation and degradation of HA/CMCS/PDA scaffolds had no adverse effects on the kidney and liver of rabbits with no inflammatory response in the implantation sites. The micro-CT and histology data suggested that the HA/CMCS/PDA scaffolds could effectively stimulate new bone formation within the femoral lacuna defect region of rabbits versus blank control at 12 weeks after implantation. Surface cortical bone was generated in the defect area in the HA/CMCS/PDA group; the defect in the blank group remained obvious. HA/CMCS/PDA scaffolds had excellent biodegradability matching the formation of new bone during implantation. In conclusion, 3D-printed HA/CMCS/PDA scaffolds have remarkable potential as a new material for repairing bone defects.
研究领域:
生物医学工程
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材料科学
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组织工程
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骨科
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2026
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IF 6.4
Wei Yang,Xiaoyu Du,Hailiang Nie,Yanqing Qu,Hongge Jia,Yongqi Sun,Elena Sashina,Jingyu Xu
CMCS@COF composite aerogels were prepared via chemical crosslinking. • Aerogel adsorbs MG/MB/CV via synergistic electrostatic, π-π, hydrogen bond. • Aerogels' 3D network significantly improves stability and repeatability. • COF introduction increased aerogel's surface area and adsorption performance. With the accelerated industrialization, dye wastewater poses severe threats to the ecological environment and human health. In this study, sulfonated COF was synthesized via a solvothermal method, then crosslinked with CMCS, and the CMCS@COF composite aerogel with a 3D network structure was fabricated via freeze drying. The maximum adsorption capacities of the as prepared material for MG, MB and CV reach 2062.95, 510.96 and 1523.21 mg·g −1 , respectively. This performance is far superior to that of pure CMCS aerogel (228.16, 167.87 and 193.96 mg·g −1 , respectively), and also significantly higher than that of most reported COF based composite adsorbents. The adsorption process conforms to the Langmuir isotherm model and the pseudo second order kinetic model, indicating that the adsorption is monolayer chemisorption, and the mechanism involves the synergistic effects of electrostatic attraction, π-π stacking and hydrogen bonding. Compared to traditional powdered covalent organic framework materials, the developed aerogel exhibits excellent structural stability and convenient solid liquid separation capability, addressing engineering bottlenecks such as powder aggregation and difficult recovery. After five consecutive adsorption desorption cycles, no significant pore collapse was observed in the structure, demonstrating excellent regeneration performance. This strategy provides a technically feasible solution for the practical application of COF based adsorbents in dye wastewater treatment, achieving a balance between high adsorption capacity and operational recyclability. Download: Download high-res image (380KB) Download: Download full-size image
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高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 6
·1236-1245
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IF 4.543
Qi Yang,Yue Zhu,Bin Luo,Fang Lan,Yao Wu,Zhongwei Gu
Glycoproteins are related to many biological activities and diseases and their effective capture and release from a complex mixture is of great significance in proteomics and diagnostics. However, to separate and target glycoproteins with high efficiency and selectivity is difficult, due to the interference of nanomaterials and other proteins in complicated samples. In this study, Fe3O4/carboxymethylated chitosan/poly(3-acrylaminophenylboronic acid) (Fe3O4/CMCS/PAAPBA) nanospheres with enhanced properties are prepared by a facile and economical method, and possess excellent morphology, uniform size (∼300 nm), high saturation magnetization (59 emu g−1), and high magnetic content (79%). Due to the introduction of PAAPBA, the Fe3O4/CMCS/PAAPBA nanospheres have been developed as a pH-responsive strategy to reversibly capture and release glycoproteins with high selectivity and recyclability in a pure protein, a model protein mixture and even a biological sample. Additionally, the Fe3O4/CMCS/PAAPBA nanospheres retained high separation efficiency (∼94%) in a pure protein system after being recycled five times, showing the great potential of the boronate functionalized, pH-stimuli-responsive, magnetic nanospheres in the biomedical field.
研究领域:
生物化学
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纳米医学
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蛋白质组学
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PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 137
·期 6
·48358
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IF 2.188
Long Chen,Xinhua Zhou,Guanquan Lin,Huayao Chen,Li Hao,Hongjun Zhou
In order to improve the utilization rate of avermectin (AVM), a complex was prepared by electrostatic self-assembly using isolated soy protein (ISP) and carboxymethyl chitosan (CMCS) for loading AVM to obtain ISP/CMCS@AVM microspheres. The encapsulation efficiency (EE), sustained release property, ultraviolet (UV) protective ability, and toxicity of the microspheres were evaluated, and the release kinetics of AVM from the microsphere at different pHs were investigated. The results demonstrated that the average particle size of ISP/CMCS@AVM was 283.95 nm, and the EE reached 88.42% for AVM after denaturation. After 70 h of exposure to UV light, the residual rate of AVM in ISP/CMCS@AVM was 78.12%, which was significantly higher than 35.18% in the AVM emulsion. Moreover, the formulation imparted pH sensitivity and sustained-release property to AVM and was consistent with the Korsmeyer–Peppas model, controlled by Fick diffusion. Finally, the insecticidal toxicity of ISP/CMCS@AVM did not differ significantly from that of unmodified AVM. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137 , 48358.
研究领域:
材料科学
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农药化学
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生物医学工程
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DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2019
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IF 3.074
Chuang Zhou,Ziming Yang,Li Zhang,Enming Dong,Zuyu He,Xianwu Liu,Chao Wang,Yan Yang,Jing Jiao,Yunhao Liu,Yu Chen,Puwang Li
In this work, carboxymethyl chitosan-graft-eleostearic acid (CMCS-g-EA) was synthesized via the amide reaction between the amino groups of carboxymethyl chitosan and the carboxyl group of eleostearic acid, and then mPEG-NH 2 was grafted to CMCS-g-EA to prepare amphiphilic polymers (mPEG-CMCS-g-EA). The chemical structures of the above conjugates were characterized by FT-IR and 1 H NMR. Both CMCS-g-EA and mPEG-CMCS-g-EA based nano-vesicles were prepared by ultrasonic self-assembly method and they exhibited a low critical aggregation concentration (CAC) of 14.97 μg/mL, 16.82 μg/mL, respectively. The spinosad-loaded mPEG-CMCS-g-EA nano-vesicles (SSD@mPEG-CMCS-g-EA NVs) were spherical in shape with an average diameter of 502.8 nm and the zeta potential of −25.60 mV. The encapsulation efficiency (EE) and drug loading content (LC) of SSD@mPEG-CMCS-g-EA nano-vesicles were 42.00%, 23.07%, respectively. In vitro release revealed that the SSD@mPEG-CMCS-g-EA nano-vesicles exhibited a sustained and pH-responsive drug release property, and could significantly enhance the photostability of spinosad . Furthermore, the toxicological tests demonstrated that the SSD@mPEG-CMCS-g-EA nano-vesicles could efficiently inhibit the growth and development of Helicoverpa armigera . These results indicated that the SSD@mPEG-CMCS-g-EA nano-vesicles were highly potential for the treatment of Helicoverpa armigera .
研究领域:
农药科学
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生物材料学
·
昆虫学
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DOI
高影响因子
危险品化学品经营许可证（带存储）