High Voltage
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2024
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IF 4.4
Shifang Yang,Zexuan Zhang,Qianpeng Hou,Yunpeng Liu,Chi Gong
The authors explore the limitations of silicone rubber (SR) in tropical environments due to its lack of antimicrobial activity. The use of a 2-methylisothiazol-3(2H)-one compound with 5-chloro-2-methylisothiazol-3(2H)-one is proposed as an efficient and environmentally friendly antimicrobial agent to enhance the fungistatic properties of SR. The fungicide added specimen was obtained by directly adding a quantitative amount of isothiazolinone to the polymer system. Through an in-depth analysis of the material properties of MCMSR, the form in which 2-methyl-4-isothiazolin-3-one/5-chloro-2-methyl-4-isothiazolin-3-one (MIT/CMIT) exists in the molecular structure of the SR crosslink system was carefully explored. The accelerated hydrolysis process of polysiloxane, which caused fungi secretion, was proven to be suppressed due to the effects of MIT/CMIT. The antimicrobial mechanism of isothiazolinone in preventing microbial growth was interpreted as the apoptosis and necrosis procedures of Aspergillus niger cells, which were verified by flow cytometry results. By choosing the appropriate concentration of isothiazolinone, the original properties of the SR were successfully maintained while effectively preventing the aggregation of isothiazolinone. This opens up a new research direction and application prospect for the antimicrobial modification of SR.
研究领域:
材料科学
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微生物学
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DOI
高影响因子
IEEE SENSORS JOURNAL
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2024
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IF 4.3
Chen Ma,Dingnan Lu,Fangyuan Zhou,Jiayue Luo,Huihui Gan,Weidong He,Pradeep U. Kurup,David Z. Zhu
Due to the almost-complete dependence on clinical diagnosis, current Helicobacter pylori (H. pylori) epidemiological statistics suffer from fragmented geographical coverage and lagged data updating worldwide. Acquiring information based on the quantitative detection of biomarkers from domestic wastewater provides a promising way for the future H. pylori epidemiological study. Here, we report wastewater-adapted electrochemical competitive immunosensor (WECI). This method focuses explicitly on detecting H. pylori cytoplasmic ureB in wastewater and contains a series of designs to overcome the challenges brought by the complicated analytical matrix. The developed WECI achieved a practical detection range from 10 pg mL $^{-{1}}$ to 5.0 ng mL $^{-{1}}$ ( ${R}^{{2}}$ = 0.996) and showed the corresponding limit of detection (LoD) and limit of quantification (LoQ) of 0.37 and 1.4 pg mL $^{-{1}}$ , respectively. It can be prepared within 6 h and kept highly sensitive for at least six weeks. When testing real wastewater samples, WECI resulted in an average biomarkers recovery rate of 70.9%, which was remarkably higher than the average of 47.0% achieved using qPCR, indicating the excellent applicability toward actual wastewater detection. Our reported WECI is the first study to quantitatively detect H. pylori ureB from wastewater, opening up new vistas for H. pylori epidemiological study.
研究领域:
医学诊断
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环境科学
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微生物学
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Tao Zhou,Zhou Liu,Xudong Ma,Chaofeng Cen,Zhangwei Huang,Yi Lu,Tiantian Kong,Cheng Qi
The essence of compartmentalization in cells is the inspiration behind the engineering of synthetic counterparts, which has emerged as a significant engineering theme. Here, we report the formation of ultra-stable water-in-water (W/W) emulsion droplets. These W/W droplets demonstrate previously unattained stability across a broad pH spectrum and exhibit resilience at temperatures up to 80℃, overcoming the challenge of insufficient robustness in dispersed droplets of aqueous two-phase systems (ATPS). The exceptional robustness is attributed to the strong anchoring of micelle-like casein colloidal particles at the PEO/DEX interface, which maintains stability under varying environmental conditions. The increased surface hydrophobicity of these particles at high temperatures contributes to the formation of thermally-stable droplets, enduring temperatures as high as 80℃. Furthermore, our study illustrates the adaptable affinity of micelle-like casein colloidal particles towards the PEO/DEX-rich phase, enabling the formation of stable DEX-in-PEO emulsions at lower pH levels, and PEO-in-DEX emulsions as the pH rises above the isoelectric point. The robust nature of these W/W emulsions unlocks new possibilities for exploring various biochemical reactions within synthetic subcellular modules and lays a solid foundation for the development of novel biomimetic materials.
研究领域:
材料科学
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生物技术
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化学工程
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胶体科学
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生物材料
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
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IF 5.9
Jinping Xu,Quanshuang Li,Bingjie Lu,Mengyao Wang,Huanchen Hou,Wangli Bao,Junyu Xia,Shibao Li,Jing Chen,Liangwen Hao
One-pot scalable synthesis of phenylboronic acid-modified AuNPs at room temperature. • Rapid Fc-oriented antibody immobilization enabled by boronate-affinity interaction. • The oriented bio-interface enhances lateral flow immunoassay sensitivity by 16-fold. • The developed LFIA enables reliable assay of fecal occult blood in clinical samples. • This method offers a promising platform for colorectal cancer point-of-care testing. The analytical performance of lateral flow immunoassay (LFIA) is largely determined by the bio-interfacial orientation of antibodies immobilized on colloidal gold nanoparticles (AuNPs). Conventional citrate-capped AuNPs (Cit-AuNPs), however, mainly rely on random physical adsorption, which often produces unfavorable antibody orientations and poor accessibility of antigen-binding sites. In addition, Cit-AuNPs typically require harsh high-temperature reflux synthesis and specialized equipment for large-scale production, increasing industrialization costs. Here, we report a facile one-pot, room-temperature strategy for the large-scale synthesis of phenylboronic acid-modified AuNPs (PBA-AuNPs), achieving 5 L batch production within 10 min. The obtained PBA-AuNPs exhibit excellent monodisperse and a uniform size of 23.5 nm, and enable an oriented antibody immobilization via boronate-affinity interactions with the carbohydrate moieties of Fc fragments. When applied to fecal occult blood (FOB) detection, the PBA-AuNPs showed a remarkable 16-fold improvement in sensitivity compared with Cit-AuNP probes. Overall, this work provides a robust, scalable nanoplatform with strong clinical translational potential for high-sensitivity LFIA diagnostics.
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PubMed
高影响因子
TALANTA
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2023
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IF 6.1
Tao Peng,Lianhua Dong,Xiaoli Feng,Yi Yang,Xia Wang,Chunyan Niu,Zhanwei Liang,Wang Qu,Qingcui Zou,Xinhua Dai,Minghua Li,Xiang Fang
More than forty antigen testing kits have been approved to response the prevalence of SARS-CoV-2 and its variant strains. However, the approved antigen testing kits are not capable of quantitative detection. Here, we successfully developed a lateral flow immunoassay based on colloidal gold nanoparticles (CGNP-based LFIA) for nucleocapsid (N) protein of SARS-CoV-2 quantitative detection. Delta strain (NMDC60042793) of SARS-CoV-2 have been cultured and analyzed by our developed digital PCR and LFIA methods to explore the relationship between N protein amount and N gene level. It indicated that the linear relationship (y = 47 ×) between N protein molecule number and N gene copy number exhibited very well (R 2 = 0.995), the virus titers and N protein amount can be roughly estimated according to nucleic acid testing. Additionally, detection limits (LODs) of nine approved antigen testing kits also have been evaluated according to the Guidelines for the registration review of 2019-nCoV antigen testing reagents. Only three antigen testing kits had LODs as stated in the instructions, the LODs of Kits have been converted into the N gene and N protein levels, according to the established relationships among virus titer vers. N gene and antigen. Results demonstrated that the sensitivity of nucleic acid testing is at least 1835 times higher than that of antigen testing. We expect that the relationship investigation and testing kits evaluation have the important directive significance to precise epidemic prevention.
研究领域:
病毒学
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2023
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IF 4.1
Hao Chi,Sheng Tian,Xiu Li,Yuchu Chen,Qiumin Xu,Qixiao Wang,Wenwan Shi,Michael Adu-Frimpong,Shanshan Tong
In order to improve the separation process of affinity chromatography that has silica as the main carrier material, we sought to construct Lipid [email protected] 4B affinity chromatography model. We extracted the lipid rafts from U251 cells with a descaler method and sucrose density gradient centrifugation. Afterwards, it was discovered via immunofluorescence that the lipid rafts contain a large amount of tropomyosin-related kinase A (TrkA) protein. Also, agarose powder in the lyophilised state was pretreated, before the lipid rafts were coupled to the agarose gel in a coupling buffer of alkaline pH. CNBr-Sepharose 4B affinity gel packing was characterised using UV spectrophotometric, immunofluorescence and scanning electron microscopic techniques, wherein and the results showed that the lipid rafts were successfully coupled to the agarose gels. Three compounds were used to verify the specific sorption of Sepharose 4B and CNBr-Sepharose 4B, which showed no specific sorption on the materials. Of note, the prepared Lipid [email protected] 4B agarose gels packed with TrkA-rich target proteins could be successfully validated for the active drug gefitinib with high affinity sorption efficiency and eluted with good recovery and reproducibility. This study broadens the range of affinity chromatography carrier materials and provides a reference for research in active drug screening.
研究领域:
生物化学
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药学
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生物技术
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分析化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2023
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IF 7.4
Xuwen Gao,Xiaoxuan Ren,Yaojia Ai,Mengwei Li,Bin Zhang,Guizheng Zou
Coreactant-free electrochemiluminescence (ECL) is promising for removing the exogenous effects of coreactant and simplify the operation procedures and setups of commercialized ECL bioassays. Herein, an electrosterically involved strategy for achieving a low-triggering-potential (+0.21 V vs Ag/AgCl) and coreactant-free ECL from dual-stabilizer-capped CdTe nanocrystals (NCs) is proposed with mercaptopropionic acid (MPA) and hexametaphosphate (HMP) as the capping agents of luminophores. Upon employing the CdTe NCs as the ECL tag for the immunoassay, all the tags in the bioconjugates of the CdTe NCs and the secondary antibody (Ab2|CdTe) as well as in the final achieved sandwich-type immunocomplexes can exhibit efficient coreactant-free ECL with an electrosterically involved triggering potential nature. The bioconjugates of Ab2|CdTe with Ab2 no more than 30 kDa, such as the thyroid stimulating hormone (30 kDa) and the recombinant pro-gastrin releasing peptide (ProGRP, 14 kDa), merely exhibit coreactant-free ECL around +0.24 V, while bioconjugates of Ab2|CdTe with an Ab2 beyond 30 kDa only give off coreactant-free ECL around +0.82 V. Due to the further enhanced electrosteric effect in sandwich-type immunocomplexes, only the ECL immunosensor with ProGRP as the target can give off coreactant-free ECL around +0.24 V. The electrosterically involved and coreactant-free ECL of CdTe NCs is consequently utilized to sensitively and selectively determine the molecular protein ProGRP, which demonstrates a wide linearity range from 0.1 to 2000 pg/mL and a low limit of detection at 0.05 pg/mL (S/N = 3). This low-triggering-potential and coreactant-free combined ECL platform indicates that engineering the surface of CdTe NCs with a protein can improve the performance of ECL tags in a protein-weight-involved electrosterical way.
研究领域:
分析化学
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生物化学
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纳米技术
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生物医学工程
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DOI
PubMed
高影响因子
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