Materials Today Bio
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2023
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IF 8.2
Yue Zhao,Lutong Song,Mengchen Li,Haoran Peng,Xinyi Qiu,Yuyang Li,Bijun Zhu,Chao Liu,Shuangshuang Ren,Leiying Miao
Vital pulp preservation, which is a clinical challenge of aseptic or iatrogenic accidental exposure of the pulp, in cases direct pulp capping is the main technology. Human dental pulp stem cells (hDPSCs) play a critical role in pulp tissue repair, but their differentiative ability could be inhibited by the potential infection and inflammatory response of the exposed pulp. Therefore, inflammatory regulation and differentiated promotion of hDPSCs are both essential for preserving living pulp teeth. In this study, we constructed a functional dental pulp-capping hydrogel by loading cerium oxide nanoparticles (CNPs) and dentin matrix protein-1 (DMP1) into an injectable Fmoc–triphenylalanine hydrogel (Fmoc-phe3 hydrogel) as CNPs/DMP1/Hydrogel for in situ drugs delivery. With a view to long-term storage and release of CNPs (anti-inflammatory and antioxidant) to regulate the local inflammatory environment and DMP1 to promote the regeneration of dentin. Results of CCK-8, LDH release, hemolysis, and Live/Dead assessment of cells demonstrated the good biocompatibility of CNPs/DMP1/Hydrogel. The levels of alkaline phosphatase activity, quantification of the mineralized nodules, expressions of osteogenic genes and proteins demonstrated CNPs/DMP1/Hydrogel could protect the activity of hDPSCs’ osteogenic/dentinogenic differentiation by reducing the inflammation response via releasing CNPs. The therapy effects were further confirmed in rat models, CNPs/DMP1/Hydrogel reduced the necrosis rate of damaged pulp and promoted injured pulp repair and reparative dentin formation with preserved vital pulps. In summary, the CNPs/DMP1/Hydrogel composite is an up-and-coming pulp-capping material candidate to induce reparative dentin formation, as well as provide a theoretical and experimental basis for developing pulp-capping materials.
研究领域:
牙科学
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生物材料
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干细胞研究
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组织工程
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
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IF 15.1
Jin-Zheng Wang,Cheng-Kun Wu,Xin-Ying Wang,Yuan-Zhi Xu,Richard Ansah Herman,Sheng Sheng,Shuai You,Jun Wang,Fu-An Wu
Edible insects are rich in oil and protein resources, and has it become increasingly important to develop them reasonably when resources are increasingly scarce. However, due to the challenges in recovering enzymes in the enzyme-assisted aqueous extraction technique and the remaining organic solvents in the extraction process based on organic solvent, it is difficult to achieve simultaneous utilization of oil and protein from edible insects. Hence, an improved enzyme-assisted aqueous extraction process based on the assistance of chitosan cross-linked Fe 3 O 4 nanoparticles (CNPs) immobilized enzymes and divalent copper ions chelation, [email protected] aqueous extraction technique, was first proposed. The hydrolysis activity was significantly increased by 6.16 times, and the value of K cat / K m was increased by 1.7 times for the results of the formation of the hydrophilic microenvironment. Furthermore, the extraction ratio of silkworm pupae oil extracted via [email protected] aqueous extraction technique could be increased to 93%, and the oil extraction rate could be maintained above 65% after 10 times reused. Improvement of enzyme-assisted aqueous extraction under the addition of divalent copper ions could achieve an ideal effect of oil extracted from silkworm pupae with less time. Additionally, the quality of silkworm pupae oil was significantly improved and the silkworm pupae protein hydrolysate also showed improved biological activity. Therefore, the enzyme-assisted aqueous extraction enhancement by trypsin immobilization and divalent copper ion chelation is a promising strategy for the green and sustainable exploration of edible insect oil and protein without the use of organic solvents.
研究领域:
食品科学
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生物化学
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纳米技术
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酶学
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可持续资源利用
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
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IF 8.5
Jianing Fu,Shaobo Li,Meizhen Xu,Ling Liu,Li Chen,Dequan Zhang
Soup is an important presence in diet, but its absorption and transport mechanism by the human body remains unclear. In this study, Caco-2 intact cell and monolayer cell models were constructed to simulate small intestine absorption on colloidal nanoparticles (CNPs) isolated from lamb soup. The intracellular localization of CNPs was viewed by laser confocal microscopy (LSCM). CNPs uptake and release pathways were explored by differences in CNPs concentrations in 5 endocytosis inhibitor models and 4 exocytosis inhibitor models. Results indicated that CNPs endocytosis by Caco-2 cells was restrained by Nystatin and Cytochalasin D, with exocytosis being inhibited by Nocodazole and Monensin. Therefore, the major absorption pathways for CNPs were caveolin-dependent endocytosis, macropinocytosis and phagocytosis. The major transport pathways were microtubule-vesicle-mediated protein transport to the membrane and transportation between the Golgi apparatus and membrane. This study may provide theoretical support for the transport mechanism of soup products in the small intestine.
研究领域:
营养学
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食品科学
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细胞生物学
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药学
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产品关联
DOI
PubMed
高影响因子
COMPOSITES SCIENCE AND TECHNOLOGY
·
2026
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IF 9.9
Tiantian Li,Weipeng Huang,Yi Li,Lijuan Zhu,Ce Liang
Homologous interfacial engineering enables atomic anchoring of P-CNPs onto C-Ti 3 C 2 T x via Ti-O-C covalent bonds. • Hierarchical architecture acts as wave confinement walls, inducing Maxwell-Wagner polarization and extended dissipation paths. • EMI SE reaches 75.44 dB at 4 wt% loading with absorption as the dominant shielding mechanism. • Composite achieves 215.4 MPa tensile strength, overcoming conductivity–mechanical integrity trade-off. Achieving a synergistic balance between electromagnetic interference (EMI) shielding and mechanical robustness in carbon fiber-reinforced polymers (CFRPs) remains a fundamental challenge due to the long-standing trade-off between electrical conductivity and mechanical integrity. Here, we report a homologous interfacial reinforcement strategy to engineer a hierarchical architecture via the atomic-scale anchoring of C-Ti 3 C 2 T X and polyimide/polydopamine-derived carbon nanoparticles (P-CNPs). The formation of Ti-O-C covalent bonds yields a continuous hybrid network that effectively suppresses MXene restacking. This hierarchical configuration acts as electromagnetic-wave confinement walls, inducing pronounced Maxwell-Wagner polarization and extending energy-dissipation pathways through multiple internal reflections. The composite achieves a high EMI shielding effectiveness (SE) of 75.44 dB at a low filler loading of 4 wt%, while maintaining an excellent tensile strength of 215.4 MPa. The homologous interfacial engineering strategy presented here offers a transformative route for simultaneously integrating mechanical robustness with absorption-enhanced EMI functionality, providing new design insights for integrated materials in aerospace and microelectronics. Download: Download high-res image (324KB) Download: Download full-size image
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DOI
高影响因子
Biomaterials Research
·
2023
·Vol. 27
·期 1
·1-21
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IF 11.3
Zhang Ying,Lei Hengyu,Wang Pengchong,Zhou Qinyuan,Yu Jie,Leng Xue,Ma Ruirui,Wang Danyang,Dong Kai,Xing Jianfeng,Dong Yalin
Background Reactive oxygen species (ROS) overproduction and excessive hypoxia play pivotal roles in the initiation and progression of ulcerative colitis (UC). Synergistic ROS scavenging and generating O2 could be a promising strategy for UC treatment.Methods Ceria nanozymes (PEG-CNPs) are fabricated using a modified reverse micelle method. We investigate hypoxia attenuating and ROS scavenging of PEG-CNPs in intestinal epithelial cells and RAW 264.7 macrophages and their effects on pro-inflammatory macrophages activation. Subsequently, we investigate the biodistribution, pharmacokinetic properties and long-term toxicity of PEG-CNPs in mice. PEG-CNPs are administered intravenously to mice with 2,4,6-trinitrobenzenesulfonic acid-induced colitis to test their colonic tissue targeting and assess their anti-inflammatory activity and mucosal healing properties in UC.Results PEG-CNPs exhibit multi-enzymatic activity that can scavenge ROS and generate O2, promote intestinal epithelial cell healing and inhibit pro-inflammatory macrophage activation, and have good biocompatibility. After intravenous administration of PEG-CNPs to colitis mice, they can enrich at the site of colonic inflammation, and reduce hypoxia-induced factor-1α expression in intestinal epithelial cells by scavenging ROS to generate O2, thus further promoting disrupted intestinal mucosal barrier restoration. Meanwhile, PEG-CNPs can effectively scavenge ROS in impaired colon tissues and relieve colonic macrophage hypoxia to suppress the pro-inflammatory macrophages activation, thereby preventing UC occurrence and development.Conclusion This study has provided a paradigm to utilize metallic nanozymes, and suggests that further materials engineering investigations could yield a facile method based on the pathological characteristics of UC for clinically managing UC.Graphical Abstract
研究领域:
纳米技术
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药理学
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炎症
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胃肠病学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 13
·2998-3004
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IF 6.331
Jia An,Yongqin Hu,Guoyi Liu,Meizhu Chen,Rubing Chen,Ying Lyu,Mengdi Yuan,Mengfei Luo,Yufei Liu
Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years. In this study, for the first time, we reported a one-pot method in situ synthesis of carbon-containing nanoparticles (CNPs) via p-aminophenol (AP) and diethylenetriamine (DETA). The CNP solution exhibits yellow and light blue fluorescence under UV-light. Moreover, the CNPs exhibited excellent photoluminescence stability even under extreme conditions. Inspired by the alkaline phosphatase (ALP)-triggered specific catalytic reaction, we constructed an ultrasensitive fluorescence and colorimetric two-channel strategy for monitoring the ALP activity. By optimizing the detection parameters, the detection limits for both fluorometric and colorimetric were 0.05 mU mL−1. Moreover, the strategy showed high specificity and was successfully applied to monitor the ALP activity level in human serum samples. The analytical strategy opened a new window for the detection of the ALP activity, screening of the ALP inhibitor, and disease diagnosis.
研究领域:
分析化学
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生物传感器
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纳米技术
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医学诊断
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）