Haojie Xie,Lin Wang,Xiaomeng Yu,Tianci Zhou,Minghui Wang,Jie Yang,Tao Gao,Genxi Li
The construction of hybrid materials is significant for the exploration of functionalities in colorimetric biosensing due to its structural designability and synergy effects. In this work, a COF-on-MOF hybrid nanomaterial has been newly synthesized for colorimetric biosensing. Experimental results reveal that on-surface synthesis of COF on MOF brings nanoscale proximity between COF and MOF, which exhibits more than two folds of peroxidase-like activity as compared to single Fe-MOF. Therefore, by using the MCA@Fe-MOF nanomaterial with the assist of a specific acetyl-peptide, MCA@Fe-MOF can serve as an efficient signal reporter for colorimetric assay of histone deacetylase (HDAC), and the limit of detection (LOD) can be as low as 0.261 nM. Looking forward, the demand for diverse and promising COF-on-MOF nanomaterials with varied functionalities is anticipated, propelling further exploration of their role in colorimetric biosensing.
17β-estradiol (E2) is a crucial estrogen that significantly influences reproductive biological processes and the development of associated diseases, highlighting the need for effective detection methods. Here, we propose a magnetic hierarchical porous material (Mag MOF@COF-Apt) through the in situ growth of a TAPB-DMTP-COF layer on a magnetic Fe-MOF core for the selective recognition and surface-enhanced Raman scattering (SERS) analysis of E2. The Mag MOF@COF-Apt features the inherent SERS-enhancing properties of Mag MOFs. Furthermore, the COF layer establishes a gap between E2 and the Mag MOF, enhancing SERS activity through the on-shell-isolated effect. Additionally, the topological structure of the COF on the MOF provides a high surface area platform for more affinity sites, and its expanded porous structure facilitates size-exclusion of interferents. Finally, E2 aptamer functionalization equips this heterojunction with the capability for the selective capture of E2. Through these multiple synergistic effects, the Mag MOF@COF-Apt assisted E2 profiling features good sensitivity (detection limit of 110.14 nmol L −1 ), strong tolerance to protein (40 mg mL −1 BSA, 40 mg mL −1 HSA) and salt (40 mg mL −1 NaCl) interference, high signal reproducibility (RSD = 7.29 %) and reusability over four cycles. The effectiveness of Mag MOF@COF-Apt based profiling was validated by successful E2 detection in human serum. This work is anticipated to advance MOF/COF hybrid substrates for SERS, providing an effective tool for E2 analysis.
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