Quick Release of Hydrogen Peroxide from Carbamide Peroxide Promotes Apoptosis of A549 Lung Cancer Cells
Graphical The present study aimed to explore new higher-H 2 O 2 -content and faster-H 2 O 2 -release rate peroxide compounds for treatment of lung cancer. The results showed that CP−B has extremely low side effects and potentially tumor treatment capabilities. Finally, it was found that CP−B up-regulate the INS-IGF2 gene and it was closely related to the PI3 K/AKT signaling pathway. Lung cancer is one of the malignant tumors with the leading morbidity and mortality worldwide. H 2 O 2 is an important oxidant, and lethal to tumor cells. The present study aimed to explore new higher-H 2 O 2 -content and faster-H 2 O 2 -release rate carbamide peroxide (CP) compounds for treatment of lung cancer. The CP added with benzoic acid termed CP−B was applied in the research. Fenton reaction was used to determine the content and rate of H 2 O 2 release by degrading organic small molecule dyes. The results showed that CP−B could be decolorized 200 mg/L RhB solution within 60 s. The phenotype was analyzed by vitro experiment the effects of the CP compound on A549 lung cancer cell line. The results showed that CP−B had a significant effect on pro-apoptosis, anti-proliferation and anti-migration of A549 cells. Using RNA seq, it was found that CP−B might up-regulate the INS-IGF2 gene and it was closely related to the PI3 K/AKT signaling pathway. Finally, in vivo experiments indicated that CP−B has extremely low side effects and potentially tumor treatment capabilities.
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