CERAMICS INTERNATIONAL
·
2016
·
IF 2.758
Huan Zhou,Saisai Hou,Mingjie Zhang,Hong Chai,Yang Liu,Sarit B Bhaduri,Lei Yang,Linhong Deng
Biphasic calcium phosphate (BCP) compositions consisting of β-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) are potential biodegradable ceramics for bone regeneration. The present work demonstrates the formation of such dense ceramics by first preparing the precursors of nano-sized, amorphous , and equiaxed calcium pyrophosphate particles, and then sintering the precursors at 900 °C to transform them into desired BCP. However, if the complex of calcium tripolyphosphate was used, only CPP could be generated. It was also observed that the incorporation of Mg 2+ had several effects on the resulting products including: (1) promoting the generation of meso-porous precipitates; (2) favoring the formation of β-TCP instead of CPP; (3) reducing the grain size and increasing the density of the sintered ceramics, and (4) enhancing the negative electric charge of the BCP surface. Thus, the as-prepared BCP ceramics can serve as potential bone substitute materials in orthopedic applications.
研究领域:
材料科学
·
生物医学工程
·
骨科
✓ 已验证
DOI
高影响因子
POLYMER COMPOSITES
·
2022
·Vol. 43
·期 3
·1476-1484
·
IF 3.531
Zheng Li,Kexin Wang,Xudong Liu,Jiaoli Hu,Jie Zhang,Guorui Yang,Ruihang Xie,Jialiang Zhang,Yuhui Ao
To enhance the interfacial adhesion of short carbon fiber (SCF) reinforced polypropylene (PP) composites, a new modified chlorinated polypropylene resin (g-CPP) was synthesized and utilized to manufacture the sizing agent for SCF. The findings of thermogravimetric analysis and differential scanning calorimetry showed that g-CPP had high thermal characteristics, with its glass transition temperature (Tg) raised by 11°C.The g-CPP sizing agent that has been developed has increased storage stability (particle size was 109.8 nm). The contact angle of SCF was reduced from 81.52° to 60.04° after sizing, and the surface free energy was raised from 29.31 mN/m soared to 47.13 mN/m. The SCF/PP composites with 1 wt% sizing agent demonstrated the best interfacial adhesion, with improvements in flexural and tensile strengths of 54% and 39%, respectively. The interfacial reinforcement mechanism of sizing agent to improve interfacial adhesion was investigated. This work proposes an attractive way for the future preparation of high-performance SCF/PP composites.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 36
·21991-21999
·
IF 3.361
Kaixuan Zhang,Xu Peng,Can Cheng,Yang Zhao,Xixun Yu
In order to solve the problem of aseptic loosening of artificial joints resulting from the wear particles of artificial joint components in total joint replacement (TJR), we synthesized a new kind of metalo-organic particle (Sr/Zn-doped CPP/GNS) using spark plasma sintering (SPS) as a filler to enhance the comprehensive performance of UHMWPE. Sr/Zn-doped CPP/GNS was interfused evenly with UHMWPE particles and cured in a hot press instrument to prepare Sr/Zn-doped CPP/GNS/UHMWPE composites. FTIR and SEM were carried out to characterize Sr/Zn-doped CPP/GNS particles. EDS was carried out to characterize Sr/Zn-doped CPP/GNS/UHMWPE. The micro-structure, hardness, impact strength, tribology and bio-activities of Sr/Zn-doped CPP/GNS/UHMWPE composite materials were also investigated. The results confirmed the effectiveness of this method. The hardness, impact strength, and tribology of the composites were enhanced by adding homodispersed Sr/Zn-doped CPP/GNS particles into UHMWPE. In the meantime, Sr/Zn-doped CPP/GNS/UHMWPE composites could significantly promote the growth of osteoblasts due to the bio-activity of Sr/Zn-doped CPP/GNS. Furthermore, the addition of Sr/Zn-doped CPP/GNS particle-fillers into UHMWPE could promote the secretion of OPG from osteoblasts and inhibit the secretion of RANKL from osteoblasts, and thus increase the OPG/RANKL ratio. All the results above showed that Sr/Zn-doped CPP/GNS/UHMWPE composites with appropriate Sr/Zn-doped CPP/GNS content possessed superior physicochemical performances and bio-properties, and could be considered as promising materials to treat aseptic loosening in total joint replacement.
研究领域:
生物医学工程
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材料科学
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骨科医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2021
·
IF 4.384
Yuping Wei,Caiying Zhang,Man Zhang,Qionghong Niu,Fengli Hui,Zi Liu,Xia Xu
In our previous study, a novel cell penetrating peptide (CPP) R7 (Arg-Arg-Arg-Arg-Arg-Trp-Trp, RRRRRWW) has been developed to help cellular internalization of paclitaxel (PTX) through the non-covalent interaction with CPP. However, the facilitation mechanism of R7 mediated PTX translocation is not clear. Here the uptake pathways of R7 and R7-PTX were investigated by in vitro test and molecular simulations. In vitro experiments reveal that both R7 and R7-PTX complex translocate through the direct translocation and clathrin mediated endocytosis and associate with the macropinocytosis pathway at high CPP concentration. The translocation of R7(0.1 mM)-PTX complex further involves the lipid raft/caveolae mediated endocytosis. The simulation results show that the synergistic effect between R7 and PTX not only changes the penetration energy barrier but also activates the macropinocytosis and lipid raft/caveolae mediated pathway, resulting in the improvement in the translocation. The presence of heparin also improves the R7 and R7-PTX translocation. These studies provide a theoretical basis for understanding PTX delivery facilitated by the synergistic effect between CPP and cargo and paves a way for CPP design.
研究领域:
药学
·
分子生物学
·
细胞生物学
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生物物理学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2024
·
IF 11.2
Xiaomei Xia,Ziyang Liu,Hongshui Wang,Yazhou Hu,Werner E.G. Müller,Xiaohong Wang,Kun Qin,Jiantao Zheng,Huan Zhou,Lei Yang,Chunyong Liang
In neurosurgery procedures, cerebrospinal fluid leakage is a commonly encountered complication. Reconstructing skull base defects with patch materials can reduce the risk of cerebrospinal fluid leakage which can lead to serious issues such as infection, meningitis, arachnoiditis, and delayed wound healing. An ideal skull base reconstruction material should not only serve as a leak-proof barrier but also promote skull base bone regeneration. To fulfill this challenge, this research designed and fabricated a Janus orthogonal bilayer nanofiber membrane (OPCL/PG-PCPP). The aligned PCL (APCL) nanofibers were constituted as the top layer to resist cerebrospinal fluid leakage, while the perpendicular PCL/gelatin (APG) fibers with calcium polyphosphate encapsulated polydopamine nanoparticles (CPP@PDA, labeled as PCPP) were designed as the bottom layer (APG-PCPP) to facilitate osteoblast migration and osteogenic differentiation. Among these, APG-1%PCPP nanofibers demonstrated the most effective induction of osteogenic differentiation in bone marrow mesenchymal stem cells (rBMSCs). Subsequent in vivo animal experiments revealed that the bone surface area (BS), bone volume fraction (BV/TV), and number of trabeculae (Tb.N) in the APG-1%PCPP group were twice as high as those in the control group, which confirmed the good osteogenic potentials. Therefore, due to its unique leak-proof and osteoinductive properties, the OPCL/PG-PCPP membrane holds promise as an applicable skull base reconstruction material in the field of neurosurgery.
研究领域:
神经外科
·
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Yuping Wei,Man Zhang,Pengfei Jiao,Xin Zhang,Ganggang Yang,Xia Xu
In our pervious study, a dual-functional peptide R7 was developed to form a complex with paclitaxel (PTX) for enhancement of PTX translocation. However, because of the unstable noncovalent bond between R7 and PTX, PTX redistributed after the introduction of heparin, leading to R7-PTX complex dissociation, further causing less PTX penetration than expected. Thus, a novel positive CPP carrier of P9 was developed to improve CPP-PTX affinity via a double-proline (Pro, P) hairpin tail and enhance PTX translocation through the reduction of translocation energy barrier, confirmed by the MM-PBSA analysis and umbrella sampling simulation. Cellular uptake study reveals that P9 can quickly translocate into the HeLa cells within 1 min and exhibits no noticeable cytotoxicity. Compared to R7, P9 is able to help PTX translocation, leading to a remarkable increase in the intracellular concentration of PTX, eventually resulting in a significant loss in tumor cell viability. In vivo experiments demonstrate that a vein injection of P9-PTX complex dramatically inhibits tumor growth. Our study provides a novel perspective for designing CPP-facilitated drug carrier to enhance antitumor efficiency.
研究领域:
药学
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生物技术
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癌症研究
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Xiaolong Wu,Mingnv Guo,Ziqi Wang,Ruiming Fang,Jiang He,Zhongqing Yang
For the purpose of further improve the efficiency of photocatalytic CO 2 reduction and realize cascade utilization of the full solar spectrum, Bi 2 MoO 6 quantum dots were in situ deposited on the surface of carbonized pomelo peel (CPP) to form Bi 2 MoO 6 @CPP 0D/3D heterojunction catalyst. With a diameter of less than 10 nm, the quantum-limited field effects confirm the successful construction of Bi 2 MoO 6 quantum dot. The band structure of Bi 2 MoO 6 @CPP 0D/3D is beneficial heterojunction , which is able to utilize not only the UV–Vis band to generate photogenerated carriers that reduce CO 2 to CO mainly, but also the IR band of the solar spectrum to increase the temperature of photothermal catalytic reaction. The experiment indicated that the photogenerated electrons generated by Bi 2 MoO 6 @CPP 0D/3D heterojunction catalysts flowed through the interface C-O-Bi bridge and migrated to the surface of CPP, forming an electron interface. The interface C-O-Bi electron bridge enhanced the separation ability of photogenerated carriers, thereby increasing the CO yield to 50.77 μmol/(g·h). Further experiment showed that the oxygen-containing groups’ concentration of CPP were ameliorated by thermal activation and acid-base activation, which increased the concentration of interface C-O-Bi electron bridge between Bi 2 MoO 6 and CPP, finally promoting the CO yield to 66.94 μmol/(g·h), which is 12.3 times greater than that of bulk Bi 2 MoO 6 .
研究领域:
材料科学
·
环境科学
·
光催化
✓ 已验证
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 18
·8476-8484
·
IF 7.394
Wenyu Li,Yajie Liu,Jianwei Du,Kefeng Ren,Youxiang Wang
Cell-penetrating peptides (CPP) have been widely developed as a strategy to enhance cell penetrating ability and transfection. In this work, octa-arginine modified dextran gene vector with pH-sensitivity was developed via host–guest interactions. α-Cyclodextrin was modified with octa-arginine (CDR), which had excellent cell penetrating ability. Dextran was selected as a backbone and modified with azobenzene as guest units by acid–labile imine bonds (Az-I-Dex). The supramolecular polymer CDR/Az-I-Dex with high a C/A molar ratio (molar ratio of CD on CDR to Az on Az-I-Dex) was unfavorable for DNA condensation. The dextran shell of CDR/Az-I-Dex/DNA polyplexes improved the stability under physiological conditions. However, once treated with acetate buffer (pH 5.4) for 3 h, large aggregates formed rapidly due to the cleavage of the dextran shell. As expected, the vector had cell viability of 80% even when the CDR concentration increased to 100 μg mL−1. Moreover, due to the effective cellular uptake efficiency, CDR/Az-I-Dex/DNA polyplexes had 6–300 times higher transfection efficiency than CDR/DNA polyplexes. It was even higher than high molecular weight PLL-based polyplexes of HEK293 T cells. Importantly, chloroquine as an endosomal escape agent could not improve the transfection of CDR/Az-I-Dex/DNA polyplexes, which indicated that the CDR/Az-I-Dex supramolecular polymer had its own ability for endosomal escape. These results suggested that the CPP-based polyplexes shelled with polysaccharide can be promising non-viral gene delivery carriers.
研究领域:
药学科学
·
分子生物学
·
基因治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Lei Sun,Xiang Zhang,Hao Yuan,Hongmin Cong,Yuxing Shi,Jialin Lu,Weilong Shi,Feng Guo
Converting waste biomass into solar evaporator foam has been recognized as an effective strategy for waste recycling and solving the problem of water shortage, but problems of salt deposition and mechanical stability seriously hinder their practical application. Herein, this work proposes a sustainable and high mechanical strength biomass foam (CDs/PPy-CPP) evaporator with zero-dimensional/one-dimensional (0D/1D) hierarchical structure by hydrophilic carbon dots (CDs) and tubular polypyrrole (PPy) are seamlessly integrated into the outer surface of the three-dimensional (3D) carbonized pomelo peel (CPP) biomass foam. The evaporator achieved a high-efficient evaporation rate of up to 2.46 kg m −2 h −1 in 3.5 wt% NaCl solution at 1 sun irradiation, and similarly exhibited outstanding salient evaporation efficiency and resistance to salt deposition in 25 wt% NaCl solution, as well as prominent swage purification ability. Excellent photothermal conversion efficiency is demonstrated attributed to the 0D/1D structure in 3D networks of the CDs/PPy-CPP evaporator, as well as superior mechanical and water transport properties based on the multilayer mesh structure and microscopic water transport pores. The research intends to offer a low-cost, energy-efficient, and environmentally friendly approach to obtain multifunctional photothermal biomass foams and demonstrate the potential applications in seawater desalinization and swage purification.
✓ 已验证
产品关联
DOI
高影响因子
Acta Pharmaceutica Sinica B
·
2017
·
IF 0
Zhili Yu,Junxiao Ye,Xing Pei,Lu Sun,Ergang Liu,Jianxin Wang,Yongzhuo Huang,Seung Jin Lee,Huining He
RNAi technology has aroused wide public interest due to its high efficiency and specificity to treat multiple types of diseases. However, the effective delivery of siRNA remains a challenge due to its large molecular weight and strong anionic charge. Considering their remarkable functions in vivo and features that are often desired in drug delivery carriers, biomimetic systems for siRNA delivery become an effective and promising strategy. Based on this, covalent attachment of synthetic cell penetrating peptides (CPP) to siRNA has become of great interest. We developed a monomeric covalent conjugate of low molecular weight protamine (LMWP, a well-established CPP) and siRNA via a cytosol-cleavable disulfide linkage using PEG as a crosslinker. Results showed that the conjugates didn't generate coagulation, and exhibited much better RNAi potency and intracellular delivery compared with the conventional charge-complexed CPP/siRNA aggregates. Three different synthetic and purification methods were compared in order to optimize synthesis efficiency and product yield. The methodology using hetero-bifunctional NHS–PEG–OPSS as a crosslinker to synthesize LMWP–siRNA simplified the synthesis and purification process and produced the highest yield. These results pave the way towards siRNA biomimetic delivery and future clinical translation.
研究领域:
药学
·
分子生物学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Qingxia Yuan,Rongyi Liang,Kunling Lv,Xiaohuo Shi,Jing Leng,Yonghong Liu,Jian Xiao,Lifeng Zhang,Longyan Zhao
Chlorella polysaccharides have been gaining increasing attention because of their high yield from dried Chlorella powder and their remarkable immunomodulatory activity. In this study, the major polysaccharide fraction, CPP-3a, in Chlorella pyrenoidosa , was isolated, and its detailed structure was investigated by analyzing the low-molecular-weight product prepared via free radical depolymerization. The results indicated that CPP-3a with a molecular weight of 195.2 kDa was formed by →2)-α-L-Ara f -(1→, →2)-α-D-Rha p -(1→, →5)-α-L-Ara f -(1→, →3)-β-D-Glc p- (1→, →4)-α-D-Glc p- (1→, →4)-α-D-Glc p A-(1→, →2,3)-α-D-Man p- (1→, →3,4)-α-D-Man p- (1→, →3,4)-β-D-Gal p- (1→, →3,6)-β-D-Gal p- (1→, and →2,3,6)-α-D-Gal p- (1→ residues, branched at C2, C3, C4, or C6 of α/β-D-Gal p and α-D-Man p , and terminated by α/β-L-Ara f , α-L-Ara p , α-D-Gal p , and β-D-Glc p . Biological assays showed that CPP-3a significantly altered the dendritic morphology of immature dendritic cells (DCs). Enhanced CD80, CD86, and MHC I expression on the cell surface and decreased phagocytic ability indicated that CPP-3a could induce the maturation of DCs. Furthermore, CPP-3a-stimulated DCs not only stimulated the proliferation of allogeneic naïve CD4 + T cells and the secretion of IFN-γ, but also directly stimulated the activation and proliferation of CD8 + T cells through cross-antigen presentation. These findings indicate that CPP-3a can promote human DC maturation and T-cell stimulation and may be a novel DC maturation inducer with potential developmental value in DC immunotherapy.
研究领域:
免疫学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Kang Wang,Zhi Li,Yiming Huang,YaotianTao,Xiao Liang,Xiaoqin Chu,Ning He,Shuangying Gui,Zhenbao Li
In this study, alkanes and sucrose esters are employed to investigate the influence of additives on lyotropic liquid crystal architecture. After molecular dynamic simulations and experiment characterization, we showed how the additives control the structure of LLCs. By controlling the polarity of additives, the phase behavior of LLCs can be engineered to form the required structure. Dissipative particle dynamics (DPD) is introduced for simulating the self-assembly of phytantriol (PT), providing intuitionistic images and structure information, which shows that additives with low-polarity complicate the internal structure of liquid crystal systems. Then the ternary phase diagrams of additives, PT, and water are constructed to systematically study the effects of additives on the phase behavior of LLCs. Consistent with DPD simulation results, there is a certain regularity in the effects of additives on the structure of liquid crystals. The difference in the structure of LLCs is due to the variability in the critical packing parameter (CPP) obtained by changing the polarity of additives. Our findings demonstrate that additives polarity is a key factor in LLCs structure, and may pave a promising avenue for novel LLCs development and translation, determining the self-assembly process and the resulting phase of LLCs.
研究领域:
材料科学
·
化学工程
·
胶体与表面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）