BMC CANCER
·
2022
·Vol. 22
·期 1
·1-12
·
IF 4.638
Chen Xueqin,Wang Yan,Liu Hancong,Zhang Jingjing,Wang Jie,Jin Xiaobao,Ma Yan
Background In our previous study, N end of the Circumsporozoite protein (CSP I-plus) modified recombinant human Endostatin (rEndostatin, endostar) (rES-CSP) was constructed, which had antiangiogenic capability and bound to hepatocellular carcinoma in vivo and in vitro. In this study, the inhibition of rES-CSP on hepatocellular carcinoma metastasis was verified in vivo and in vitro, and its possible mechanism was explored. Methods Firstly, the impact of rES-CSP on the migration, adhesion of hepatoma cell HCCLM3 was identified by wound healing, transwell, and on metastasis of orthotopic xenograft model was identified in nude mouse. Then the expression of metastasis-associated molecules (MMP2, E-cadherin, integrinβ1) and angiogenesis-related factors (VEGFA) in vitro and in vivo were detected by real-time PCR, western blotting, immunohistochemistry. Results Finally, we found that rES-CSP could inhibit the migration and invasion of HCCLM3, and decrease tumor metastasis and growth in nude mouse orthotopic xenograft models. The tumor inhibiting rates of rES-CSP and Endostar were 42.46 ± 5.39% and 11.1 ± 1.88%. The lung metastasis rates of the control, Endostar and rES-CSP were 71, 50, and 42.8%, respectively. Compared with Endostar, rES-CSP significantly down-regulated the expression of VEGFA and integrinβ1. Heparin, a competitive inhibitor of CSP I-plus, which can be bind to the highly-sulfated heparan sulfate proteoglycans (HSPGs) over-expressed in liver and hepatocellular carcinoma, alleviated the down-regulation of VEGFA and integrinβ1. Conclusions These indicate that rES-CSP may play a role in inhibiting tumor growth and metastasis by down-regulating the angiogenic factor VEGF and the metastasis-related molecules or by interfering with HSPGs-mediated tumor metastasis.
研究领域:
肿瘤学
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分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science Advances
·
2020
·Vol. 6
·期 45
·
IF 13.117
Jiang Yan,Qinglei Meng,Xiaojun Shen,Bingfeng Chen,Yang Sun,Junfeng Xiang,Huizhen Liu,Buxing Han
Phenol is an important commodity chemical in the industry, which is currently produced using fossil feedstocks. Here, we report a strategy to produce phenol from lignin by directly deconstructing C sp2 –C sp3 and C–O bonds under mild conditions. It was found that zeolite catalyst could efficiently catalyze both the direct C sp2 –C sp3 bond breakage to remove propyl structure and aliphatic β carbon–oxygen (C β– O) bond hydrolysis to form OH group on the aromatic ring. The yield of phenol could reach 10.9 weight % with a selectivity of 91.8%. In this unique route, water was the only reactant besides lignin. A scale-up experiment showed that 4.1 g of pure phenol could be obtained from 50.0 g of lignin. The reaction pathway was proposed by a combination of control experiments and density functional theory studies. This work opens the way for producing phenol from lignin by direct transformation of C sp2 –C sp3 and C–O bonds in lignin.
研究领域:
催化
·
绿色化学
·
生物质转化
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化学工程
·
有机化学
✓ 已验证
DOI
高影响因子
Microbiology Spectrum
·
2025
·
IF 3.8
Yue-yue Zhang,Ding-rong Zhang,Zhen-quan Yang,Lei Yuan,Ya Zhao,Yuan-song Zhang
Cronobacter sakazakii is an opportunistic pathogen that can contaminate infant formula, resulting in meningitis and neurological disorders in infants. Bacteriophages have been consistently identified as effective inhibitors of pathogenic bacteria. In this study, a lytic phage, Csp-D17, was isolated from environmental wastewater, and its characterization, genomics, and application were analyzed. Transmission electron microscopy revealed that the phage belonged to the order Caudoviricetes within the family Ackermannviridae. The phage Csp-D17 exhibited notable stability across varied thermal and pH conditions, with an optimal multiplicity of infection of 0.01 and a lysis capacity of 40 plaque-forming units per infected cell. Evaluation of its host range revealed lytic activity against 4 out of 11 tested strains of C. sakazakii (36.4%). The frequency of insensitive mutations in C. sakazakii D17 to phage Csp-D17 was 3.8 × 10−7. High-dose phage treatment was more effective in inhibiting the growth of C. sakazakii D17 in the powdered infant formula model, which was correlated with its short latency period, high lysis amount, and low frequency of insensitive mutations in the host bacteria. The results demonstrated that phage Csp-D17 was not lysogenic. Furthermore, the genomic study of phage Csp-D17 revealed that the genome structure of this phage was a double-stranded DNA molecule with a length of 143,081 base pairs, a guanine + cytosine content of 46.42%, 233 open reading frames (the average gene length is 553 base pairs), and it does not contain any genes related to virulence or lysogenicity. Furthermore, the genetic function, expression mechanism, and phylogenetic diversity of Csp-D17 were analyzed by genomic comparison. This study provides a theoretical basis for developing novel biocontrol agents based on phage control of C. sakazakii.
研究领域:
微生物学
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病毒学
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食品安全
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基因组学
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分子生物学
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噬菌体治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2023
·
IF 8.7
Azhar Abbas Khosa,Xinyue Han,C.Y. Zhao
CaCO 3 /CaO thermochemical energy storage (TCES) system has been widely studied on laboratory scale for its potential in concentrated solar power (CSP) plants. However, there is still need for the large-scale experiments based on which a detailed analysis can be obtained to improve this system for real-time applications. In this study, we developed a packed bed reactor capable of operating at high temperature and pressure providing the carbonation and decarbonation reactions of CaCO 3 /CaO reaction pair. The heat storage process is studied with the aspect of different decarbonation temperatures and different sample sizes (amount of sample in grams) placed in the reactor. Furthermore, we compared the heat storage performance of pure and composite samples. The heat release process is performed by varying the concentration of CO 2 and the sample size of the loaded material. The results showed that the heat storage rate and efficiency improve by increasing the temperature of the decarbonation, implying that this reaction must be carried out above 925 °C for better performance. The heat is released smoothly and at a good rate when the concentration of CO 2 is high in the reactor. Also, carbonation reaction (heat release process) depicts that CaCO 3 /CaO TCES system has a great potential to be used as a heat pump and is capable of upgrading the temperature of the released heat from lower to higher value.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Energy Materials
·
2025
·
IF 5.5
Ruiqing Zhang,Guanyu Li,Shiyi Chen,Wenguo Xiang
The successful commercialization of concentrated solar power (CSP) plants necessitates the development of efficient energy storage systems to ensure the on-demand power generation during solar transients. In recent years, the redox cycling of doped CaMnO3−δ has emerged as a promising approach for thermochemical energy storage (TCES) in CSP applications. This study aims to enhance the thermochemical energy storage capability of CaMnO3−δ by co-doping with Sr and Co at the A- and B-sites, respectively, forming (SrxCa1–x)(CoxMn1–x)O3–δ. Two doping levels (x = 0.05 and 0.10) were investigated. XRD and SEM–EDS analyses confirmed that Sr and Co were homogeneously incorporated into the perovskite lattice, yielding porous microstructures in both compositions. Notably, evaluation of the thermochemical energy storage (TCES) densities of these codoped perovskites under diverse thermal energy storage conditions revealed that although the two materials exhibited different heat storage processes, comparable TCES densities were ultimately achieved. In particular, under favorable thermochemical energy storage conditions (1100 °C, pO2 = 10–5 atm), the thermochemical energy storage densities of Sr0.05Ca0.95Co0.05Mn0.95O3–δ and Sr0.10Ca0.90Co0.10Mn0.90O3–δ achieved 459 kJ/kgABO3 and 470 kJ/kgABO3, respectively. However, in the subsequent redox cycle tests, Sr0.10Ca0.90Co0.10Mn0.90O3–δ demonstrated a stronger reoxidation capability compared to Sr0.05Ca0.95Co0.05Mn0.95O3–δ. Compared to the undoped material, both co-doped samples exhibited significant improvements in the TCES density and thermal stability. Moreover, the isothermal redox reaction rate measurements and the analysis revealed the rapid redox reaction rate in both materials, providing valuable insights for the development of advanced perovskite-based thermochemical energy storage systems.
研究领域:
材料科学
·
能源存储技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2016
·Vol. 128
·期 3
·1783-1792
·
IF 1.781
Zhang Zhaoli,Yuan Yanping,Ouyang Liping,Sun Qinrong,Cao Xiaoling,Alelyani Sami
Nanofluids of Li 2 CO 3 –Na 2 CO 3 –K 2 CO 3 improved by three nano-Al 2 O 3 samples are firstly prepared by means of two-step aqueous method to enhance thermal properties for high-temperature heat transfer, when used as heat transfer fluids and thermal energy systems for concentrating solar power systems. Specific heat of ternary carbonates containing Al 2 O 3 of 0.2, 0.4, 0.8, 1.0, 1.4 and 2.0 mass% is measured, and nanofluids with 1.0 mass% of 20-nm Al 2 O 3 , 1.0 mass% of 50-nm Al 2 O 3 and 0.8 mass% of 80-nm Al 2 O 3 are selected as superior candidates. The maximum enhancement of specific heat is 18.5% in solid and 33.0% in liquid, 17.9% in solid and 22.7% in liquid, 13.2% in solid and 17.5% in liquid for nanofluids containing 20-, 50- and 80-nm Al 2 O 3 . Thermal conductivity is, respectively, improved by 23.3, 28.5 and 30.9% under the addition of Al 2 O 3 . New chemical bonds and crystals are scarcely formed in composites through FT-IR and XRD determination. SEM images certify that nano-Al 2 O 3 are homogeneously mixed into nanofluids and this structure may be a critical incentive for enhancing thermal properties. There are no significant changes with respect to the heat flow, melting/freezing point and latent heat after the 30 circles of determination. Briefly, it can be speculated that these nanofluids will exhibit tremendous potential in the coming applications of heat transfer and thermal storage for concentrating solar power systems.
研究领域:
材料科学
·
能源工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2023
·Vol. 106
·期 7
·4116-4127
·
IF 3.9
Shuangli Dong,Yanqi Jia,Yongbao Xiao,Qinyuan Zhang
Despite the significant technological applications of laser glasses, the quantitative prediction and optimization of their properties, particularly the spectroscopic properties, remain challenging. Here we address this problem by regarding the nearest-neighboring congruently melting compounds as the “component and structural motifs” based on the phase diagram approach. The composition–structure–property (CSP) relationships of Nd 3+ -doped ternary phosphate glasses are investigated. Spectroscopic properties are quantitatively predicted with an error of less than 10% compared to the experimental results. In particular, the fluorescence lifetime ( τ m ) can be accurately calculated using the phase diagram approach, which is difficult to be predicted previously. Furthermore, the CSP databases with over 1000 compositions are established for the optimization of commercial laser glasses. This study enriches the CSP database of traditional laser glass and provides guiding significance for future research.
研究领域:
材料科学
·
光学
·
化学
✓ 已验证
DOI
高影响因子
Insects
·
2024
·Vol. 15
·期 7
·501
·
IF 2.7
Ningna Yin,Dan Shen,Yinlan Liang,Pengfei Wang,Yonghe Li,Naiyong Liu
Simple SummaryThe swallowtail butterflyPapilio xuthusis a specialized herbivorous insect, with its larvae solely utilizing the Rutaceae plants as hosts. The host seeking and orientation of this butterfly mainly rely on chemosensory-related genes expressed in the antennae, including chemosensory proteins (CSPs). Here, we characterized the putative roles of a female-antenna-biased PxutCSP19 inP. xuthusassociated with the perception of host volatiles and the sequestering of insecticides. This study examined the key functions of PxutCSP19 in the interactions of six host-derived odorants and eight insecticides. Notably, an extended N-terminus of PxutCSP19 did not significantly affect the binding specificities of this protein to ligands with high affinities. Our findings provide insight into the binding mechanisms of PxutCSP19 inP. xuthusto host volatiles and insecticides.AbstractChemosensory protein (CSP) genes significantly enriched in the female antennae are potential molecular candidates for mediating female oviposition behaviors. In this study, we presented the interaction mechanisms of a female-antenna-biased PxutCSP19 inPapilio xuthusto 47 host volatiles, four biopesticides and 24 synthetic insecticides. Using a bioinformatics-based homology search, 22 genes orthologous to PxutCSP19 were identified from 22 otherPapiliobutterflies with high sequence identities to each other (73.20~98.72%). Multiple alignment analyses revealed a particularly extended N-terminus ofPapilioCSP19s (an average of 154 residues) compared to insects’ typical CSPs (approximately 120 residues). The expression profiles indicated that PxutCSP19 was significantly enriched in the female antennae, with a 31.81-fold difference relative to the male antennae. In ligand-binding assays, PxutCSP19 could strongly bind six host odorants with high affinities, ranging from dissociation constant (Ki) values of 20.44 ± 0.64 μM to 22.71 ± 0.73 μM. Notably, this protein was tuned to a monoterpenoid alcohol, linalool, which generally existed in the Rutaceae plants and elicited electrophysiological and behavioral activities of the swallowtail butterfly. On the other hand, PxutCSP19 was also capable of binding eight insecticides with stronger binding abilities (Ki< 12 μM) compared to host odorants. When an extended N-terminal region of PxutCSP19 was truncated into two different proteins, they did not significantly affect the binding of PxutCSP19 to ligands with high affinities, suggesting that this extended N-terminal sequences were not involved in the specificity of ligand recognition. Altogether, our study sheds light on the putative roles of PxutCSP19 enriched in the female antennae ofP. xuthusin the perception of host volatiles and the sequestering of insecticides, and it complements the knowledge of butterfly CSPs in olfaction and insecticide resistance.
研究领域:
昆虫学
·
分子生物学
·
化学生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2025
·
IF 3.5
Xin Yu,Zhengyi Zhao,Guofei Zhang,Sirong Li,Songnan Qin,Yanzhi Yang,Zhiyong Yan,Yuhan Yang,Xuechun Xiao
This study systematically investigates the relationship between the solvent used, the crystal morphology of MnCo 2 O 4.5 , and its thermocatalytic activity using the solvent thermal method. By tweaking the urea content in the solvothermal process and the ethanol/glycol ratio in the reaction solvents, the shape and size of the MnCo 2 O 4.5 precursor were controlled by altering the crystal growth direction and rate. The MnCo 2 O 4.5 material with “litchi” core-shell structure with maximum specific surface area and abundant pore structure is prepared when the addition amount of urea is 27 mmol and the solvent is ethanol/glycol (20:40 mL). This material reduces the high thermal decomposition temperature ( T HTD ) of ammonium perchlorate (AP) from 473.48 °C to 274.86 °C and increases the exothermic amount from 888.26 J g −1 to 1654.03 J g −1 . Compared to pure AP (296.8 kJ mol −1 ), the activation energy ( E a ) of AP + 2.0 wt% MnCo 2 O 4.5 catalytic material decreases to 61.1 kJ mol −1 . Applying the “litchi” core-shell structured MnCo 2 O 4.5 to the hydroxyl-terminated polybutadiene composite solid propellant (HTPB-CSP) enhances combustion intensity, reducing the time to peak combustion from 390 ms for pure AP to 265 ms and ignition delay from 35 ms to 21 ms. The experimental results indicate that the “litchi” MnCo 2 O 4.5 boosts AP thermal decomposition and improves HTPB-CSP combustion.
研究领域:
材料科学
·
燃烧与推进
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2026
·
IF 4.1
Mao Shuai,An Zhoujian,Du Xiaoze,Ding Yong,Zhang Dong,Fu Jian
Nanofluids obtained by the incorporation of nanoparticles into ternary carbonates be capable of enhancing the low heat transfer and the capacity for thermal storage as well as the poor fluidity of carbonate. In this study, 31.5 mass% Na 2 CO 3 -31.5 mass% Li 2 CO 3 -37 mass% K 2 CO 3 was used as the ternary carbonate. Nanofluids were prepared utilize the high-temperature melting technique by dispersing nanoparticles of different particle sizes, concentrations, and types in carbonate solution. The thermophysical properties of nanofluids were measured employing X-ray diffractometer, viscosity analyzer, scanning electron microscopy, etc. The findings indicated that among the selected nanoparticles, SiO 2 containing 1.0 mass% magnitude of particles of 30 nm was the most effective in enhancing the heat transfer, heat storage, and flow properties of the ternary carbonate. From the fitted curves, the incorporation of SiO 2 increased the solid state specific heat capacity with 6.25%, the liquid form by 6.0%. The liquid phase exhibited a 27.8% improvement of thermal conductivity, and the viscosity of nanofluids was below 10 cp with increasing temperature, and the viscosity was less than 3 cp at 500 °C. After undergoing 100 h of high-temperature insulation and 100 cycles of thermal shock with large temperature difference, nanofluids exhibited a melting temperature change of only 1.39% and 1.19%, demonstrating excellent initial stability. Subsequent testing will involve extended periods of high-temperature insulation and thermal shock cycles with large temperature differences to ensure this nanofluid truly meets practical application requirements.
✓ 已验证
产品关联
DOI
高影响因子
Energy Storage Materials
·
2019
·
IF 0
Liang Teng,Yimin Xuan,Yun Da,Xianglei Liu,Yulong Ding
The thermochemical energy storage based on Calcium looping (CaL) process shows great potential for the application in the 3 rd generation Concentrated Solar Power (CSP) compared to other high-Temperature heat storage schemes. However, due to the inherent low solar absorptance of CaCO 3 , the surface heating mode is widely adopted in the conventional CaL-CSP system, which causes large thermal resistance and severe radiative heat loss . Herein, we propose achieving direct solar absorption in the CaL-CSP system through enhancing the CaCO 3 ’s ability to capture thermal energy from the concentrated solar irradiation . Efforts are devoted to design and fabricate a modified CaL material by doping CaCO 3 with some materials with high solar absorptance . In order to obtain a highly stable CaL, the cyclic performance of the composite material is investigated and optimized. The calcium gluconate ((Ca(C 6 H 11 O 7 ) 2 )) was used as the precursor while fabricating the porous CaCO 3 , and the Mn–Fe oxides were doped into CaCO 3 through two different doping processes. The experimental results indicate that the proposed material obtains the solar absorptance of ∼90%. The heat release efficiency (equal to carbonation activity) remains over 92% in 60 cycles, which is much higher than that of the commercial CaCO 3 powder (20% after 60 cycles). The proposed calcium-based thermochemical energy storage material is expected to dramatically improve both the solar utilization efficiency and cyclic stability of the integrated CaL-CSP system.
研究领域:
储能技术
·
太阳能
·
材料科学
·
化学工程
·
可再生能源
✓ 已验证
产品关联
DOI
MOLECULAR GENETICS AND GENOMICS
·
2022
·Vol. 297
·期 3
·801-815
·
IF 2.98
Zhang Yonglei,Gao Shanshan,Zhang Ping,Sun Haidi,Lu Ruixue,Yu Runnan,Li Yanxiao,Zhang Kunpeng,Li Bin
Eugenol, a plant-derived component possessing small side effects, has an insecticidal activity to Tribolium castaneum ; however, the underlying molecular mechanisms of eugenol acting on T. castaneum are currently unclear. Here, a nerve conduction carboxylesterase and a detoxifying glutathione S-transferase were significantly inhibited after eugenol exposure, resulting in the paralysis or death of beetles. Then, RNA-sequencing of eugenol-exposed and control samples identified 362 differentially expressed genes (DEGs), containing 206 up-regulated and 156 down-regulated genes. RNA-seq data were validated further by qRT-PCR. GO analysis revealed that DEGs were associated with 1308 GO terms of which the most enriched GO terms were catalytic activity, and integral component of membrane; KEGG pathway analysis showed that these DEGs were distributed in 151 different pathways, of which some pathways associated with metabolism of xenobiotics or drug were significantly enriched, which indicated that eugenol most likely disturbed the processes of metabolism, and detoxication. Moreover, several DEGs including Hexokinase type 2 , Isocitrate dehydrogenase , and Cytochrome b-related protein , might participate in the respiratory metabolism of eugenol-exposed beetles. Some DEGs encoding CYP , UGT , GST , OBP , CSP , and ABC transporter were involved in the xenobiotic or drug metabolism pathway, which suggested that these genes of T. castaneum participated in the response to eugenol exposure. Additionally, TcOBPC11/ TcGSTs7 , detected by qRT-PCR and RNA-interference against these genes, significantly increased the mortality of eugenol-treated T. castaneum , providing further evidence for the involvement of OBP/GST in eugenol metabolic detoxification in T. castaneum . These results aid eugenol insecticidal mechanisms and provide the basis of insect control.
研究领域:
昆虫学
·
分子生物学
·
基因组学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）