ANNALS OF AGRICULTURAL SCIENCE
·
2023
·
IF 5.9
Hong Hu,Liang Chen,Ying Huang,Kunping Wang,Xi Bai,Hongbin Pan
This work aimed to study the effect of phloretin on antioxidant profiles through nuclear factor erythroid 2–related 2 (Nrf2)-DNA interactions in the liver of heat-stressed broilers by Cleavage Under Targets and Tagmentation combined with high-throughput sequencing (CUT&Tag-seq). One hundred and sixty 22-day-old Arbor Acres broilers were divided into four groups: a normal temperature group (NT: 22.8–23.4 °C and 46–55.8 % humidity) was fed a basal diet and three high temperature groups (China Summer condition: 29.7–31.7 °C and 77.9–94.8 % humidity) were fed the basal diet supplemented with 0 (HT group), 100 mg/kg (LT group) and 200 mg/kg (PT group) phloretin. Liver samples were taken from 42-day-old broilers. High temperature condition increased ( P ≤ 0.05) the malondialdehyde (MDA) level, but decreased ( P ≤ 0.05) the activities and mRNA expression levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and Nrf2. Dietary 200 mg/kg phloretin decreased ( P ≤ 0.05) the MDA level, but increased ( P ≤ 0.05) the activities and mRNA expression levels of GSH-Px, SOD, CAT and Nrf2 in the liver of heat-stressed broilers. CUT&Tag-seq showed that 4042 DEPs were up-regulated, while 2322 DEPs were down-regulated in the PT group compared with the HT group. The Nrf2-binding gene of DEPs contained SOD3, CAT, SOD2, and p38 mitogen-activated protein kinase. Most TOP20 significant KEGG pathways were associated with “Environmental Information Processing”. Summary, phloretin could improve oxidative damage by Nrf2 and its downstream DNA-sites including genes and Nrf2-bingding non-coding RNAs in the liver of heat-stressed broilers.
研究领域:
动物科学
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分子生物学
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基因组学
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生物信息学
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DOI
高影响因子
Brain Sciences
·
2023
·Vol. 13
·期 2
·334
·
IF 3.3
Shanshan Lin,Chengrui Wang,Zhiwen Li,Xiu Qiu
The pathological mechanisms of neural tube defects (NTDs) are not yet fully understood. Although the dysregulation of histone modification in NTDs is recognized, it remains to be fully elucidated on a genome-wide level. We profiled genome-wide H3K27me3 and H3K27ac occupancy by CUT&Tag in neural tissues from ICR mouse embryos with benzo[a]pyrene (BaP)-induced NTDs (250 mg kg−1) at E9.5. Furthermore, we performed RNA sequencing (RNA-seq) to investigate the regulation of histone modifications on gene expressions. Gene ontology and KEGG analysis were conducted to predict pathways involved in the development of NTDs. Our analysis of histone 3 lysine 27 modification in BaP-NTD neural tissues compared to BaP-nonNTD revealed 6045 differentially trimethylated regions and 3104 acetylated regions throughout the genome, respectively. The functional analysis identified a number of pathways uniquely enriched for BaP-NTD embryos, including known neurodevelopment related pathways such as anterior/posterior pattern specification, ephrin receptor signaling pathway, neuron migration and neuron differentiation. RNA-seq identified 423 differentially expressed genes (DEGs) between BaP-NTD and BaP-nonNTD group. The combination analysis of CUT&Tag and RNA-seq found that 55 DEGs were modified by H3K27me3 and 25 by H3K27ac in BaP-NTD, respectively. In the transcriptional regulatory network, transcriptional factors including Srsf1, Ume6, Zbtb7b, and Cad were predicated to be involved in gene expression regulation. In conclusion, our results provide an overview of histone modifications during neural tube closure and demonstrate a key role of genome-wide alterations in H3K27me3 and H3K27ac in NTDs corresponding with changes in transcription profiles.Keywords:neural tube defects;CUT&Tag;RNA-seq;epigenetics
研究领域:
表观遗传学
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神经科学
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分子生物学
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基因组学
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DOI
PubMed
高影响因子
SOLID STATE SCIENCES
·
2018
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IF 1.861
Kun Chai,Hongyi Li,Mingyuan Zhu,Bin Dai
By the introduction of Rb + with large ionic radius and rigid SiO 4 unit into borate , a noncentrosymmetric crystal, RbBSi 2 O 6 polymorph has been obtained. Its structure was determined by single crystal X-ray diffraction technique , and it can be described as a three dimensional (3D) framework constructed by the Si/BO 4 tetrahedra via corner-sharing O atoms, the Rb atoms reside in the tunnels and large cavities of the 3D framework. In addition, the optical properties of RbBSi 2 O 6 and CsBSi 2 O 6 , including IR spectra , UV–Vis–NIR diffuse reflectance spectra, have been reported in this work. Both RbBSi 2 O 6 and CsBSi 2 O 6 reveal short deep ultraviolet (UV) cutoff edges (below 180 nm and 185 nm, respectively), so they may have potential application in deep UV fields. Furthermore, the first-principle theoretical studies on band structure and density of states have also been performed for RbBSi 2 O 6 .
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DOI
高影响因子
Gels
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2023
·Vol. 9
·期 2
·158
·
IF 4.6
Shilei Zhu,Dongdong Yan,Lin Chen,Yan Wang,Fengbo Zhu,Yanan Ye,Yong Zheng,Wenwen Yu,Qiang Zheng
The Kirigami approach is an effective way to realize controllable deformation of intelligent materials via introducing cuts into bulk materials. For materials ranging from ordinary stiff materials such as glass, ceramics, and metals to soft materials, including ordinary hydrogels and elastomers, all of them are all sensitive to the presence of cuts, which usually act as defects to deteriorate mechanical properties. Herein, we study the influence of the cuts on the mechanical properties by introducing “dispersed macro-scale cuts” into a model tough double network (DN) hydrogel (named D-cut gel), which consists of a rigid and brittle first network and a ductile stretchable second network. For comparison, DN gels with “continuous cuts” having the same number of interconnected cuts (named C-cut gel) were chosen. The fracture tests of D-cut gel and C-cut gel with different cut patterns were performed. The fracture observation revealed that crack blunting occurred at each cut tip, and a large wrinkle-like zone was formed where the wrinkles were parallel to the propagation direction of the cut. By utilizing homemade circular polarizing optical systems, we found that introducing dispersed cuts increases the rupture force by homogenizing the stress around the crack tip surrounding every cut, which reduces stress concentration in one certain cut. We believe this work reveals the fracture mechanism of tough soft materials with a kirigami cut structure, which should guide the design of advanced soft and tough materials along this line.Keywords:double-network hydrogels;cut dispersion;fracture;toughness
研究领域:
材料科学
·
力学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SAFETY
·
2026
·Vol. 46
·期 3
·e70071
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IF 2.7
Yifei Wang,Pan Li,Liyao Gu,Jun Ma,Lin Ye,Joanna Trafiałek,Rowaida K. S. Khalil,Shuo Zhang,Shoukui He
Eugenol, a major bioactive compound present in various essential oils with substantial antibacterial activity, has limited application in food industries due to its low water solubility. To address this limitation, a microemulsion system was developed utilizing Tween 80 as a surfactant and lactic acid as a co-surfactant to facilitate the delivery of eugenol. The resulting microemulsion underwent physicochemical characterization, antibacterial evaluation, and sanitation trials on fresh-cut lettuce. Phase analysis revealed three distinct types of microemulsions with stability and dispersibility: water-in-oil with 0%–20% water, bicontinuous with 30%–60% water, and oil-in-water with 70%–90% water. Antibacterial assays indicated minimum inhibitory concentrations of 0.5 mg/mL for Staphylococcus aureus and 0.25 mg/mL for Escherichia coli , along with minimum bactericidal concentrations of 1 and 0.5 mg/mL, respectively. Furthermore, the microemulsion at 1 mg/mL exhibited rapid bactericidal effects against both bacterial strains, achieving a population reduction of over 6-log units within 10 min. In refrigerated fresh-cut lettuce sanitation experiments, microemulsion concentrations of 0.25, 0.5, and 1 mg/mL effectively suppressed the number of total microbes, mold, and yeast, with 0.5 mg/mL treatment being most effective in maintaining lettuce quality and freshness. Additionally, these treatments contributed to the preservation of vitamin C content, chlorophyll level, and color stability. Overall, the results demonstrate that eugenol microemulsions hold strong potential as green sanitizers for vegetables, offering a promising avenue to enhance food safety and quality.
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
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2022
·
IF 7.425
Ting Du,Xiang Li,ShaoChi Wang,Zehui Su,Hao sun,Jianlong Wang,Wentao Zhang
Nature-derived chemicals have recently gained increased attention to settle down the challenges in the food industry. Quercetin has long been used as a natural medicine but its photoactivity has been neglected. In this work, by combining photodynamic bacteria inactivation (PDI) with an edible coating (Pectin/Quercetin) derived from FDA-approved chemicals, extend shelf-life and protected commercial quality of fresh-cut apples were achieved. Firstly, the potential photoactivated antibacterial performance of Quercetin (a natural plant flavonoid) was clarified with the treatment of a simulated sunlight lamp, realizing antibacterial efficacy of 100 % towards S. aureus (50 min) and L. monocytogenes (80 min) with light treatment. To develop safe and effective preservation of fresh-cut apples, Pectin/Quercetin edible coatings with 100 μmol/L quercetin were adopted. The results showed that the prepared edible coatings form a protective barrier over the surface of apples, effectively resisting bacterial infection and extending shelf life to 10 days while maintaining good commercial quality (including preferable color, keeping 100 % hardness, 80 % sugar content and 17.3 % weightlessness rate). Therefore, the prepared light-driven Pectin/Quercetin in this work has the potential to develop as fresh-cut fruit preservation technology.
研究领域:
食品科学
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植物化学
·
采后技术
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DOI
PubMed
高影响因子
CELLULAR SIGNALLING
·
2024
·
IF 4.4
Jingyi Li,Ziyu Chen,Mingming Jin,Xuefeng Gu,Yuhan Wang,Gang Huang,Weiming Zhao,Changlian Lu
Lactylation, a newly identified post-translational modification, is uncertain in its implication in triple-negative breast cancer (TNBC). In this study, we analyzed 60 TNBC samples using immunohistochemical staining and revealed elevated levels of pan-lactylated proteins and specific histone H4K12 lactylation in tumor tissues, correlating with TNBC progression. Lactate exposure in TNBC cell lines significantly induced lysine lactylation at the H4K12 site, leading to alterations in gene profiles and reduced apoptosis. These effects were attenuated by DCA or sodium Oxamate, inhibitors of endogenous lactate production. Gene sequencing showed an increase in Schlafen 5 (SLFN5) expression in TNBC cells treated with Oxamate, contrasting with the effects of lactate exposure. Analysis of TNBC tissues showed a negative correlation between H4K12 lactylation and SLFN5 protein levels. Overexpression of SLFN5 countered the effects of lactate on apoptosis and tumor growth, highlighting its pivotal role in TNBC malignancy. CUT&Tag sequencing indicated that lactylated H4K12 potentially binds to the SLFN5 promoter region. Luciferase reporter assays further verified that lactate-induced suppression of SLFN5 promoter activity is mediated by wild-type H4K12, but not by its R or A mutants, verified by both in vitro and in vivo apoptosis detection in response to lactate and Oxamate stimulation. These results establish that H4K12 lactylation, induced by lactate in TNBC cells, specifically suppresses SLFN5 expression, contributing to TNBC malignancy. Our findings illuminate a critical histone lactylation-dependent carcinogenic pathway in TNBC.
研究领域:
肿瘤学
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分子生物学
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癌症研究
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DOI
PubMed
高影响因子
MATERIALS LETTERS
·
2019
·
IF 3.019
Na Li,Xuefang Lu,Qun Jing,Ping Wei,Lu Qi,Zhongqi Jiang,Zhaohui Chen
Phosphates are another new family of exploring deep-ultraviolet nonlinear optical materials. As one material, the simpler the composition is the more favorable synthesis and application is. In this work, a simple alkaline earth metal phosphate β -Ba(PO 3 ) 2 was synthesized by high temperature solid phase method. It is worth mentioning that it has short cut-off edge (<190 nm) and relatively large birefringence (Δn = 0.032 @ 1064 nm) in phosphates and the optical anisotropy and birefringence can be enhanced by optimizing the basic anion arrangement. Combining with theoretical analysis, the relationship between structure and performance is further elaborated. In additional, thermal properties, linear optical and nonlinear optical properties for β -Ba(PO 3 ) 2 were also investigated.
研究领域:
材料科学
·
光学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2020
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IF 7.182
Hangyue Zhao,Lei Wang,Tarun Belwal,Yunhong Jiang,Dong Li,Yanqun Xu,Zisheng Luo,Li Li
This work was designed to develop the chitosan-based melatonin layer-by-layer assembly (CMLLA) via the inclusion method. The structural characterizations and interaction present in CMLLA were investigated by the scanning electron microscope (SEM), X-ray diffraction (XRD) and Fourier Transform-Infrared spectroscopy (FTIR). The ratio of chitosan (CH) to carboxymethylcellulose (CMC) greatly influenced the mechanical properties, including the tensile strength, moisture content and color performance. Results showed that both antioxidant and antimicrobial properties of CMLLA were enhanced with the addition of melatonin (MLT). Furthermore, it was demonstrated that the CMLLA with 1.2 % (w/v) CH, 0.8 % (w/v) CMC and 50 mg/L MLT better contributed to the delay of chlorophyll degradation and the maintenance of shelf-life quality. Results from this study might open up new insights into the approaches of quality improvement of postharvest fresh products by incorporating the natural antioxidant compounds into natural polymers.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Food Science & Nutrition
·
2023
·
IF 3.9
Guoqin Li,Xinxin Wang,Hongmei Zhu,Guifeng Li,Junjie Du,Xiaoqing Song, Erihemu
Fresh-cut potato browning is a severe problem in the potato processing industry. Ascorbic acid, L-cysteine, hydrogen sulfide (H 2 S), and nitric oxide (NO) have been reported to reduce the browning in fresh-cut vegetables and fruits. We compared the effect of each food additive at its commonly used concentration on fresh-cut potato browning in order to choose a highly efficient treatment and explore its mechanism. Fresh-cut potato slices were immersed in 0.3 mmol L −1 ascorbic acid, 0.7 mmol L −1 L-cysteine, 0.7 mmol L −1 H 2 S, or 2.0 mmol L −1 NO for 10 min and stored at 4°C until the measurements finished. Results showed that the ascorbic acid and L-cysteine treatments showed less browning than the control treatment, while the H 2 S and NO treatments did not. Ascorbic acid increased total phenolic content, polyphenol oxidase (PPO) and peroxidase (POD) activities, while L-cysteine decreased PPO and POD activities with no change in total phenolic content. In addition, these two treatments did not influence respiration rate, weight loss, or rot index. In conclusion, ascorbic acid (0.3 mmol L −1 ) and L-cysteine (0.7 mmol L −1 ) can be valuable means to control fresh-cut potato browning. Ascorbic acid inhibits the browning mainly by reducing quinones back to phenolic compounds, but L-cysteine inhibits the browning mainly by decreasing PPO and POD activities.
研究领域:
食品科学
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采后技术
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植物生理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 1
·566-575
·
IF 3.713
Shuang Guo,Yue Ma,Hao Liang,Xiaoyan Zhao,Dan Wang
Yellowing is a main discoloration in fresh-cut yam, which reduces consumer acceptance and, hence, the commodity value. Bisdemethoxycurcumin has been demonstrated to play important roles in fresh-cut yam yellowing. Considering the pharmacological properties and application in fruits and vegetables of diphenyliodonium iodide (DPI), the effects and mechanism of DPI treatment on bisdemethoxycurcumin accumulation in fresh-cut yam were analysed. The results showed that total bisdemethoxycurcumin in fresh-cut yam treated with DPI was markedly inhibited due to the significantly weakened phenylpropanoid pathway and bisdemethoxycurcumin biosynthesis. DPI was found to suppress the activities of major enzymes (phenylalanine ammonia lyase, PAL, cinnamate-4 hydroxylase, C4H; 4-coumarate coenzyme A ligase, 4CL), limit the levels of PAL, C4H and 4CL transcription and lower the contents of related metabolites in fresh-cut yam. Moreover, treatment with DPI inhibitor restrained the downstream transcription level of curcumin synthase (CURS), which had a significant impact on bisdemethoxycurcumin synthesis. Thus, our results confirmed that DPI was essential for inhibiting bisdemethoxycurcumin accumulation in fresh-cut yam through suppressed phenylpropanoid pathway and bisdemethoxycurcumin biosynthesis.
研究领域:
植物科学
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食品科学
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生物化学
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Yiming Zhao,Yue Chen,Xiaoyu Song,Yuan Li,Limin Zhang,Yeqiao Meng,Xiaolai Zhang,Xiaoyong Qiu,Jun Huang
Many soft animals like starfish have developed armors to protect their soft bodies in order to survive in harsh environment. Inspired by this fact, special hydrogels with self-growing protective armors are developed by allowing sodium acetate to crystallize on hydrogel surface. Poly(acrylic acid) chains limit the crystalline region to the surface of the hydrogel by decreasing the pH value and limiting the movement of ions, and then creating a set of tough armor. This armor-protected hydrogel is able to withstand a high pressure (> 78 MPa) under cutting and prevent the penetration of sharp objects. Interestingly, the unique stimulated-precipitation mechanism allows the armor to repair itself after damage. Besides, the surface of hydrogel changes from “sticky” to “slippery”, which also helps to improve its protective ability. Moreover, the armor helps to retrain water in hydrogel network, and significantly improve the mechanical properties of hydrogels (maximum compressive stress >18 MPa, compressive strain > 90%). In addition, the hydrogel can keep soft and work durably at extreme temperatures (−50 °C and 80 °C) due to the high salt concentration. This study provides an innovative approach for designing armor-protected hydrogels with great potential in engineering applications such as actuators and sensors for harsh environments.
研究领域:
材料科学
·
仿生学
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机械工程
·
化学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）