FOOD HYDROCOLLOIDS
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2014
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IF 4.28
Sha Cai,Meng Zhao,Yapeng Fang,Katsuyoshi Nishinari,Glyn O. Phillips,Fatang Jiang
Emulsification/internal gelation is an effective encapsulation method to protect probiotics from adverse environment. In this paper, microcapsules of Lactobacillus acidophilus CGMCC1.2686 were prepared by emulsification and gelation of alginate solutions with slow release of Ca 2+ ions from calcium carbonate (CaCO 3 ) and calcium disodium ethylenediaminetetraacetate (Ca-EDTA). Physical characterization of the two types of microcapsules showed that alginate-Ca-EDTA microcapsule was more uniform in size than alginate-CaCO 3 , with a span factor of 0.96 and 1.20, respectively. Mechanical measurements demonstrated that alginate-CaCO 3 microcapsule was more robust and elastic, which was supported by its denser structure as observed by electron scanning microscopy. Bacteria encapsulation yields in the two microcapsules were similar, 37.9% for alginate-CaCO 3 and 36.9% for alginate-Ca-EDTA. However, alginate-CaCO 3 microcapsule exhibited much higher cell survivals in both simulated gastric juice and bile salts solution (22.2% and 2.6*10 −2 %, respectively) than alginate-Ca-EDTA (7.1% and 0%, respectively), indicating a more effective protection of L. acidophilus CGMCC1.2686. The protection efficiency was discussed in relation to the mechanical properties of the microcapsules and the calcium sources used.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2020
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IF 3.644
Shao Fei,Yu Lei,Zhang Yang,An Chuanfeng,Zhang Haoyue,Zhang Yujie,Xiong Yi,Wang Huanan
Microfluidics-based alginate microgels have shown great potential to encapsulate cells in a high-throughput and controllable manner. However, cell viability and biological functions are substantially compromised due to the harsh conditions for gelation, which remains a major challenge for cell encapsulation. Herein, we presented an efficient and biocompatible method by on-chip triggered gelation to generate microfluidic alginate microgels for single-cell encapsulation. Two calcium complexes of calcium–ethylenediaminetetraacetic acid (Ca-EDTA) and calcium–nitrilotriacetic (Ca-NTA) as crosslinkers for triggered gelation of alginate were compared and investigated for feasible application. By triggered release of Ca2+ ions from the calcium complex via adding acetic acid in the oil phase, the alginate precursor in the aqueous droplets can be crosslinked to form alginate microgels. Although using Ca-EDTA and Ca-NTA both achieved on-chip gelation, Ca-NTA led to significantly higher cell viability since the dissociation of Ca2+ ions from Ca-NTA can be obtained using less concentration of acid compared to Ca-EDTA. We further demonstrated the functionality of encapsulated mesenchymal stem cells (MSCs) in alginate microgels prepared using Ca-NTA, as evidenced by the osteogenesis of encapsulated MSCs upon inductive culture. In summary, our study provided a biocompatible strategy to prepare alginate microgels for single-cell encapsulation which can be further used for applications in tissue engineering and cell therapies.
研究领域:
生物技术
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组织工程
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细胞生物学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2020
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IF 7.053
Xin-Sheng Qin,Zhi-Gang Luo,Xiao-Lin Li
In this study, we demonstrated the role of W 1 /O/W 2 double emulsion with alginate-Ca-EDTA system on the gastrointestinal digestion viability of Lactobacillus Plantarum strain liquid. The W 1 /O/W 2 double emulsion was prepared by WPI-EGCG covalent conjugate nanoparticles with compartmented multiple-emulsion nature. The effects of preparation parameters containing polyglycerol polyricinoleate content, oil fraction, and WPI-EGCG covalent conjugate particle concentration on the formation of W 1 /O/W 2 double emulsions were systematically studied. Then, the rheological properties of alginate-Ca-EDTA system in the external aqueous phase of the W 1 /O/W 2 double emulsions as the pH changes were investigated. The W 1 /O/W 2 double emulsion maintained in the hydrogel state at pH below 4.0 because of Ca 2+ coupled with G residues of alginate . In contrast, the W 1 /O/W 2 double emulsion transformed to solution state at neutral pH condition, which caused the dissociation of Ca 2+ from the Ca-alginate hydrogel and resulted in Ca-EDTA chelation. A pH-sensitive carrier based on an alginate-Ca-EDTA system in a set-type W 1 /O/W 2 double emulsion model was exploited by regulating the release of Ca 2+ . Based on the pH-sensitive alginate-Ca-EDTA system and middle oil phase resistance, such a double emulsion supplied a colon-targeted release carrier for the Lactobacillus Plantarum strain liquid encapsulation into inner aqueous phase with the functional protection effect. The results offer a promising strategy for probiotics strain liquid encapsulation of double emulsion delivery systems in oral applications of foods (such as yogurt, fruit juice or cheese) and pharmaceuticals.
研究领域:
食品科学
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制药技术
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Yannan Song,Shuo Li,Huifang Chen,Xinyi Han,Gregory J. Duns,Wubliker Dessie,Wufei Tang,Yimin Tan,Zuodong Qin,Xiaofang Luo
There are several factors that contribute to the mortality of people who suffer from unmanageable bleeding. Therefore, the development of rapid hemostatic materials is necessary. Herein, novel rapid hemostatic composite sponges were developed by incorporation of kaolin (K) into carboxymethyl chitosan (CMCS)/sodium alginate (SA) via a combination of methods that includes ionic crosslinking, polyelectrolyte action, and freeze-drying. The CMCS/SA-K composite sponges were cross-linked with calcium ions provided by a sustained-release system consisting of D-gluconolactone (GDL) and Ca-EDTA, and the hemostatic ability of the sponges was enhanced by loading the inorganic hemostatic agent-kaolin (K). It was demonstrated that the CMCS/SA-K composite sponges had a good porous structure and water absorption properties, excellent mechanical properties, outstanding biodegradability , and biocompatibility. Simultaneously, they exhibited rapid hemostatic properties, both in vitro and in vivo . Significantly, the hemostatic time of the CMCS/SA-K 60 sponge was improved by 82.76 %, 191.82 %, and 153.05 %, compared with those of commercially available gelatin sponges in the rat tail amputation, femoral vein, and liver injury hemorrhage models respectively, indicating that its hemostatic ability was superior to that of commercially available hemostatic materials. Therefore, CMCS/SA-K composite sponges show great promise for rapid hemostasis .
研究领域:
材料科学
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生物材料
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止血
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）