APPLIED SURFACE SCIENCE
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2021
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IF 6.707
You Wu,Yangyang Duan,Juan Qiu,Xiang Gao,Houyi Ma
The aim of this work is to develop an eco-friendly pH-responsive intelligent coating with long-term corrosion protection by a convenient method. Herein, an eco-friendly and long-term pH-responsive intelligent anti-corrosion coating (BTA-CaCO 3 @TA coating) is fabricated through the addition of a hydrophilic pH-responsive release material (BTA-CaCO 3 @TA microspheres) into a waterborne epoxy resin coating. The electrochemical impedance spectroscopy results indicated that BTA-CaCO 3 @TA microspheres could endow the coating with long-term corrosion protection. The |Z| f =0.01Hz values of the BTA-CaCO 3 @TA coating after 130 days of immersion were still higher than those of the pure waterborne epoxy resin coating at the beginning of immersion. The excellent long-term protection performance was related to the good dispersibility of BTA-CaCO 3 @TA microspheres in the waterborne coatings, the TA conversion film and the BTA inhibitor protection film, which formed on the steel surface in situ. This synergistic strategy demonstrated its future application for protecting metals from corrosion.
研究领域:
材料科学
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腐蚀与防护
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS RESEARCH
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2020
·Vol. 35
·期 18
·2427-2440
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IF 2.502
Tingyu Yang,Yu Wu,Xiaoqing Yue,Cuiyan Wang,Jianbin Zhang
The synthesis of antibacterial biomaterial with specific functions responsive to specific bacterial growth environments is of significant importance to achieve effective sterilization and reduce the resistant bacteria. Herein, inspired by biomineralization, we develop a one-pot, threonine (Thr)-mediated biomineralization method using a CO2 bubbling procedure to green, simply and quickly prepare vaterite CaCO3 microspheres as a platform for antibacterial Sanguinarine (SAN) delivery. The loading capacity of vaterite CaCO3 microspheres for SAN drugs reached 159.8 mg/g, corresponding to the loading efficiency of 83.7%. And for the first time, a novel Sanguinarine@calcium carbonate (SAN@CaCO3) organic–inorganic hybrid antibacterial biofilm was constructed by using vaterite CaCO3 microspheres with pH-responsive and high SAN drug-loading. Importantly, the film showed bacteria-triggered, pH-responsive SAN release properties and strong bactericidal ability (96.19%) for Staphylococcus aureus (S. aureus). Meanwhile, it also had antibacterial capabilities in real environments. In 7 days, it can significantly inhibit the adhesion and growth of bacteria in the air. The biomineralized synthetic vaterite CaCO3 microspheres and the application in the construction of pH-responsive antibacterial biofilm have bright future in resisting bacterial infections and reducing the production of resistant bacteria.
研究领域:
材料科学
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生物技术
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药学
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生物医学工程
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产品关联
DOI
高影响因子
Biomaterials Science
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2019
·Vol. 7
·期 9
·3614-3626
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IF 5.251
Yihong Gong,Yiling Zhang,Zhinan Cao,Feng Ye,Zefeng Lin,Yan Li
Injectable scaffolds have attracted much attention because of their minimum surgical invasiveness. However, limited osteogenic induction property and low mechanical properties hampered their application in bone tissue engineering. CaCO3 microspheres, which possess osteoinductivity, rough surfaces and specific binding sites for BMP-2, were first fabricated; after BMP-2 uploading, microspheres were further entrapped in fibrin-glue hydrogel. CaCO3 microspheres were co-functionalized with casein and heparin. To obtain a high encapsulation of heparin and thus BMP-2 uploading, along with controlled release and simultaneous maintenance of the presence of vaterite which had osteogenic induction property, fabrication parameters were optimized and microspheres were characterized using XRD, FITR and SEM. The formed CaCO3 had a microsphere morphology of ∼1 μm. Both vaterite and calcite phases were present and the relative amount of calcite phase increased with the amount of heparin. Sample 25 mM_4-1Hep with the highest loading amount of heparin was selected as carrier for BMP-2 and BMP-2 loaded CaCO3 microspheres were further entrapped in fibrin-glue hydrogel (FC-B). For the as-prepared composite hydrogel, mechanical properties were characterized and the presence of CaCO3 significantly elevated the tensile strength; controlled release of BMP-2 was sustained until day 21. Based on ALP activity, alizarin red staining and RT-PCR, in vitro osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was found to be significantly enhanced under induction of FC-B. Rabbit tibia bone defect model was applied to evaluate its in vivo performance. After implantation for 4 weeks, presence of composite hydrogel was observed in defects. After 8 weeks, bone defects of FC-B group were nearly completely healed. Using the fact that autologous scaffolds can be derived based on fibrin-glue hydrogel, the well-designed BMP-2 loaded fibrin-glue composite hydrogel demonstrated good potential in bone tissue engineering.
研究领域:
组织工程
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生物材料
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骨再生
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药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2023
·
IF 3.9
Peng-Cheng Chen,Yao-Dan Zhang,Pu Zheng,Ruo-Xi Sun,Dan Wu
The efficiency of multi-enzyme immobilization is often influenced by the carrier and immobilization techniques employed. In this work, we proposed a ternary enzyme cascade reaction system composed of β-glucosidase (β-G), glucose oxidase (GOD) and horseradish peroxidase (HRP). CaCO 3 microspheres served as a template for in situ immobilization, while poly (allylamine hydrochloride) (PAH) and sodium poly (4-styrene sulfonate) (PSS) were utilized to create PAH-CaCO 3 and PSS-CaCO 3 templates for layer-by-layer multi-enzyme immobilization. The effects of template structure, glutaraldehyde (GA) addition, ethylenediaminetetraacetic acid (EDTA) concentration, and the structure of layer-by-layer assembly were systematically investigated. Our results indicated that an assembly of PSS-CaCO 3 --PAH--β-G--HRP--GOD presented simultaneous high enzyme loading and relative activity. The loadings of β-G, GOD and HRP were 75.2 ± 2.0%, 87.7 ± 4.4% and 85.5 ± 3.2%, respectively, while the relative activities were 81.8 ± 2.7%, 93.6 ± 4.7% and 90.6 ± 3.1%, respectively. Kinetic parameters confirmed that the reaction rate of the immobilized ternary enzymes in this assembly was close to that of the free enzymes. This work provides insights for constructing other multi-enzyme cascade reaction systems using immobilization techniques.
研究领域:
生物技术
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酶工程
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材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）